Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,448,157 | T→C | N95S (AAC→AGC) | prmB ← | N5‑glutamine methyltransferase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,448,157 | 0 | T | C | 98.3% | 222.4 / ‑6.8 | 59 | N95S (AAC→AGC) | prmB | N5‑glutamine methyltransferase |
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (22/36); total (22/37) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCGCTACCAGTACACATATCTAAAATATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCT > NC_000913/2448010‑2448301 | ccGCTACCAGTACACATATCTAAAATATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTgg < 2:145102/151‑1 (MQ=255) aCCAGTACACATATCTAAAATATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCa < 1:564788/149‑1 (MQ=255) aCCAGTACACATATCTAAAATATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCa < 2:133895/149‑1 (MQ=255) cAGTACACATATCTAAAATATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGt > 1:480556/1‑150 (MQ=255) cAGTACACATATCTAAAATATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTa < 2:729980/151‑1 (MQ=255) aGTACACATATCTAAAATATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTa > 1:325456/1‑150 (MQ=255) acaTATCTAAAATATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCAc > 2:523635/1‑151 (MQ=255) atatCTAAAATATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCTCGCTTTTCTGGTCAGGTAAGCCACCg < 2:566699/151‑1 (MQ=255) aaaaTATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATg < 1:752999/150‑1 (MQ=255) aaaaTATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGc > 1:232303/1‑151 (MQ=255) aaaaTATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGc > 2:442750/1‑151 (MQ=255) gcGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTg < 2:664535/149‑1 (MQ=255) cGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCTCCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGa < 1:763971/149‑1 (MQ=255) cGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGAcg > 1:375645/1‑151 (MQ=255) ttgcttgcTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACgcgg > 2:271193/1‑151 (MQ=255) gcttgctGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACgcggc < 1:560128/150‑1 (MQ=255) ttgctGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGcggcgg > 1:225988/1‑150 (MQ=255) aaGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCAcgcg > 2:761956/1‑150 (MQ=255) aGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGtt < 2:740653/151‑1 (MQ=255) cGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTcaac < 2:112054/151‑1 (MQ=255) aTTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACg < 1:393321/151‑1 (MQ=255) tttATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACgg > 2:381234/1‑151 (MQ=255) tATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGgt < 2:365135/150‑1 (MQ=255) aTTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGgtgt < 2:592717/151‑1 (MQ=255) gttgATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGtttt > 1:599277/1‑151 (MQ=255) ttgATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGtttt < 2:603045/150‑1 (MQ=255) tgATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGttttt < 2:198880/150‑1 (MQ=255) gATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCg < 1:459266/151‑1 (MQ=255) cAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTg < 2:61363/151‑1 (MQ=255) gTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGa < 1:279505/151‑1 (MQ=255) tCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTgggg < 1:672677/150‑3 (MQ=255) cACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGt > 1:91269/1‑151 (MQ=255) cAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAg < 1:2843/151‑1 (MQ=255) cAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCAACGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAg < 2:646028/151‑1 (MQ=255) aTCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGa > 1:353702/1‑150 (MQ=255) ggCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGAcgcg < 1:673473/151‑1 (MQ=255) aGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGt < 1:503679/151‑1 (MQ=255) cgGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATCGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTgcgc > 1:353572/1‑151 (MQ=255) aCCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCatat < 2:221015/151‑1 (MQ=255) cAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCt < 1:350014/151‑1 (MQ=255) cgcgTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCgg < 1:523659/151‑1 (MQ=255) cgTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGATTGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGaa < 2:17593/151‑1 (MQ=255) cATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATc < 1:465907/151‑1 (MQ=255) aCGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCg < 2:483767/151‑1 (MQ=255) aaTTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAgg > 1:282680/1‑150 (MQ=255) aaTTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGt < 1:675448/151‑1 (MQ=255) ttCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAgg > 1:205610/1‑148 (MQ=255) ttCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGt > 2:433466/1‑149 (MQ=255) tCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGTGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTag < 1:79589/150‑1 (MQ=255) tCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCTAGCGGCAGGTa > 2:228756/1‑149 (MQ=255) ccGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGa > 1:268060/1‑149 (MQ=255) gCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGc < 2:645699/151‑1 (MQ=255) gCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGc < 1:270168/151‑1 (MQ=255) cAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGc > 1:414965/1‑150 (MQ=255) aCCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAacac > 1:407238/1‑151 (MQ=255) ccACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATCGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACAcc < 2:353558/151‑1 (MQ=255) cACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCa < 1:459203/151‑1 (MQ=255) aCGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACAcc > 1:117275/1‑149 (MQ=255) gCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCt < 1:164328/151‑1 (MQ=255) | CCGCTACCAGTACACATATCTAAAATATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCT > NC_000913/2448010‑2448301 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |