Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,474,212	A→G	T61A (ACT→GCT)	yfdQ →	CPS‑53 (KpLE1) prophage; uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,474,212	0	A	G	100.0%	38.9 / NA	12	T61A (ACT→GCT)	yfdQ	CPS‑53 (KpLE1) prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/2);  total (10/2)

TATCAGTAAGATTGGTGCCGATGCTGTGGCATTGCCTGAGAATTTCCGCATTCATGATCTGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCACTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAAC  >  NC_000913/2474106‑2474352                                                                                                           |                                                                                                                                             tATCAGTAAGATTGGTGCCGATGCTGTGGCATTGCCTGAGAATTTCCGCATTCATGATCTGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGc                                                                                                   >  2:98892/1‑150 (MQ=255)  aTCAGTAAGATTGGTGCCGATGCTGTGGCATTGCCTGAGAATTTCCGCATTCATGATCTGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCa                                                                                                  <  1:369815/150‑1 (MQ=255)            ttGGTGCCGATGCTGTGGCATTGCCTGAGAATTTCCGCATTCATGATCTGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCAc                                                                                       >  2:382401/1‑151 (MQ=255)                                           tttCCGCATTCATGATCTGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGc                                                          <  1:477573/149‑1 (MQ=255)                                           tttCCGCATTCATGATCTGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGc                                                          >  2:477550/1‑149 (MQ=255)                                                                         tttaaATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTAtt                          >  2:505574/1‑151 (MQ=255)                                                                              aTCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATtgatg                      >  2:356851/1‑150 (MQ=255)                                                                                 gCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAAcc                  >  1:624031/1‑151 (MQ=255)                                                                                   ttccgtttccgtGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAAcc                  >  2:170559/1‑149 (MQ=255)                                                                                          tccgtGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACg          >  2:624798/1‑150 (MQ=255)                                                                                             gtggtgCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGc         >  1:568369/1‑148 (MQ=255)                                                                                                  gcgcTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAac  >  1:678711/1‑150 (MQ=255)                                                                                                           |                                                                                                                                             TATCAGTAAGATTGGTGCCGATGCTGTGGCATTGCCTGAGAATTTCCGCATTCATGATCTGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCACTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAAC  >  NC_000913/2474106‑2474352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑