Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,555,942	T→C	A153A (GCA→GCG)	eutL ←	putative ethanol utilization carboxysome structural protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,555,942	0	T	C	100.0%	177.9 / NA	50	A153A (GCA→GCG)	eutL	putative ethanol utilization carboxysome structural protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/26);  total (24/26)

CTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGTGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAGGAATGCCGTATCTTGCGCGTCATTAGCCCACTGGAAAGCTGCGCCATTTTCAATATGCG  >  NC_000913/2555806‑2556087                                                                                                                                         |                                                                                                                                                  ctTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGc                                                                                                                                     >  2:676483/1‑151 (MQ=255)   ttACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGcc                                                                                                                                    <  1:274474/150‑1 (MQ=255)       acGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCTATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCgg                                                                                                                               <  1:662116/151‑1 (MQ=255)            gCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGc                                                                                                                          >  2:752146/1‑151 (MQ=255)              cTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGcc                                                                                                                         >  1:197875/1‑150 (MQ=255)                   tACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCg                                                                                                                    >  1:181533/1‑150 (MQ=255)                     ccGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGa                                                                                                                 <  2:40343/151‑1 (MQ=255)                       ggTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGTCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGAt                                                                                                                <  1:308235/150‑1 (MQ=255)                       ggTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATc                                                                                                               <  2:409756/151‑1 (MQ=255)                          tAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCcg                                                                                                            >  2:645094/1‑151 (MQ=255)                          tAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCcg                                                                                                            >  2:538943/1‑151 (MQ=255)                                 gctgcCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTtg                                                                                                     >  1:234614/1‑151 (MQ=255)                                    gcCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTtga                                                                                                    <  1:281744/149‑1 (MQ=255)                                    gcCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTtga                                                                                                    <  2:597678/149‑1 (MQ=255)                                     ccGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATCCGCCATCGGATCGCCAAGCGTGATCCCGGCGGGtgat                                                                                                   >  1:69027/1‑149 (MQ=255)                                        agtagtTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATgagag                                                                                              <  1:617931/151‑1 (MQ=255)                                           agtTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATa                                                                                           <  1:663148/151‑1 (MQ=255)                                            gtTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATaa                                                                                          <  1:676578/151‑1 (MQ=255)                                                gTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAAc                                                                                      <  1:216992/151‑1 (MQ=255)                                                   tCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCg                                                                                    <  1:498278/150‑1 (MQ=255)                                                   tCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCg                                                                                    <  2:50223/150‑1 (MQ=255)                                                    cAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCgg                                                                                   >  1:600167/1‑150 (MQ=255)                                                    cAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGt                                                                                  >  1:271949/1‑151 (MQ=255)                                                      gACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCATCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTAc                                                                                <  2:645496/151‑1 (MQ=255)                                                            ggCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGaa                                                                           <  1:5532/150‑1 (MQ=255)                                                             gCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCTCGGCGGTTGTAAAGAGGAAAAAACCGGTACGCGAAAc                                                                         >  2:405626/1‑151 (MQ=255)                                                               gggACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACt                                                                        >  2:377499/1‑150 (MQ=255)                                                                  aCAGAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACa                                                                     <  2:156443/150‑1 (MQ=255)                                                                    aTAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACAtgt                                                                  >  1:331631/1‑151 (MQ=255)                                                                        gTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCa                                                              <  1:720009/151‑1 (MQ=255)                                                                        gTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCa                                                              <  2:152600/151‑1 (MQ=255)                                                                        gTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCa                                                              <  1:387977/151‑1 (MQ=255)                                                                         tCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGcc                                                               >  2:481926/1‑149 (MQ=255)                                                                          cGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAgg                                                            >  1:104483/1‑151 (MQ=255)                                                                          cGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAgg                                                            >  1:748313/1‑151 (MQ=255)                                                                            ccAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAGGaa                                                          <  2:240374/151‑1 (MQ=255)                                                                                    aCGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAGGAATGCCGTa                                                   <  2:364691/150‑1 (MQ=255)                                                                                    aCGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAGGAATGCCGTAt                                                  >  1:117983/1‑151 (MQ=255)                                                                                      gTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCCGGAATGCCGGATc                                                 >  2:320889/1‑150 (MQ=255)                                                                                         ggCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAGGAATGCCGTATCTTgc                                             <  1:341825/151‑1 (MQ=255)                                                                                          gCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAGGAATGCCGTATCTTgcg                                            <  2:23251/151‑1 (MQ=255)                                                                                                    gggCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAGGAATGCCGTATCTTGCGCGTCATTAGc                                  <  1:676524/151‑1 (MQ=255)                                                                                                         gCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAGGAATGCCGTATCTTGCGCGTCATTAGCCCACt                             >  1:674845/1‑151 (MQ=255)                                                                                                          cATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGGTAAGAACCGGTACTCGGAACTACATGTGCCAGGAATGCTGTATCTTTCGCGTCATTAGCCCACTg                            >  1:12442/1‑151 (MQ=255)                                                                                                          cATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAGGAATGCCGTATCTTGCGCGTCATTAGCCCACTg                            <  2:662896/151‑1 (MQ=255)                                                                                                            tCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAGGAATGCCGTATCTTGCGCGTCATTAGCCCACTgg                           >  1:6169/1‑150 (MQ=255)                                                                                                              aaTGCCTTAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGGGATAAGAACCGGTACGCGAAACTAAATTGGCAAGGTATTCCGTATCTTGCGCGTCCTTAACCCACTGGa                          >  1:418538/1‑149 (MQ=255)                                                                                                                     tAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCCAGCGCGATCCCGGCGGTTGATGGGAGAGAAGAACCGGGACGCGAAACTACATGTGCCAGGAATGCCGGATCTTGCGGGTCATTAACCCACTGGAAAGCTg                    >  2:49137/1‑148 (MQ=255)                                                                                                                        gTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAGGAATGCCGTATCTTGCGCGTCATTAGCCCACTGGAAAGCTGCGCCAt              <  1:741001/151‑1 (MQ=255)                                                                                                                                 aGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAGGAATGCCGTATCTTGCGCGTCATTAGCCCACTGGAAAGCTGCGCCCTTTTCAatat     >  2:435860/1‑151 (MQ=255)                                                                                                                                  gcggcggcgCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAGGAATGCCGTATCTTGCGCGTCATTAGCCCACTGGAAAGCTGCGCCATTTTCAatat     <  1:509242/150‑1 (MQ=255)                                                                                                                                    ggcggcGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAGGAATGCCGTATCTTGCGCGTCATTAGCCCACTGGAAAGCTGCGCCATTTTCAATATgcg  >  1:557417/1‑151 (MQ=255)                                                                                                                                         |                                                                                                                                                  CTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGTGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAGGAATGCCGTATCTTGCGCGTCATTAGCCCACTGGAAAGCTGCGCCATTTTCAATATGCG  >  NC_000913/2555806‑2556087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑