Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,704,016	A→G	T16T (ACT→ACC)	rnc ←	RNase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,704,016	0	A	G	100.0%	118.7 / NA	32	T16T (ACT→ACC)	rnc	RNase III
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/29);  total (3/29)

CATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAAGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGACCTGATATACATGGTTCTGCTAACTGCTT  >  NC_000913/2703871‑2704139                                                                                                                                                  |                                                                                                                            cATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCACCAGTTCCTGATGATTAAAGgtgt                                                                                                                         <  2:24291/150‑1 (MQ=255) cATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGgtgt                                                                                                                         <  2:642252/150‑1 (MQ=255)                 aCGGTGATAAAGTGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGa                                                                                                        <  2:436912/151‑1 (MQ=255)                         aaaGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGat                                                                                                 <  2:502765/150‑1 (MQ=255)                         aaaGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGat                                                                                                 <  1:735328/150‑1 (MQ=255)                          aaGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGatta                                                                                               <  1:168333/151‑1 (MQ=255)                                    gCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATgg                                                                                     <  2:639020/151‑1 (MQ=255)                                         aaCGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTc                                                                                <  1:600996/151‑1 (MQ=255)                                            gTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCAt                                                                              <  2:30594/150‑1 (MQ=255)                                              aGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGc                                                                            <  2:83751/150‑1 (MQ=255)                                                   aGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATAcc                                                                      <  1:597127/151‑1 (MQ=255)                                                   aGAATAGAGTCGCCTAAAAATTCTAAACACTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATAcc                                                                      >  2:539613/1‑151 (MQ=255)                                                          agTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCaataaat                                                               <  2:370884/151‑1 (MQ=255)                                                             cGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAataaataa                                                             <  1:467285/150‑1 (MQ=255)                                                             cGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAataaataa                                                             <  1:243433/150‑1 (MQ=255)                                                                    aaaTTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTca                                                     <  1:118646/151‑1 (MQ=255)                                                                            aaCGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCa                                             <  1:106637/151‑1 (MQ=255)                                                                              cGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGc                                           <  2:228141/151‑1 (MQ=255)                                                                              cGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGc                                           <  2:279480/151‑1 (MQ=255)                                                                                    ttATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACg                                      >  1:310105/1‑150 (MQ=255)                                                                                        gTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACa                                 <  1:591793/151‑1 (MQ=255)                                                                                        gTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACa                                 <  1:101241/151‑1 (MQ=255)                                                                                           tACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGa                               <  1:273417/150‑1 (MQ=255)                                                                                            aCTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGAcc                             <  1:29601/151‑1 (MQ=255)                                                                                                ctgGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGACGCCGATTAATTACGATGGGGTTCTTGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACCAAACAGACCTGa                          <  2:207671/150‑1 (MQ=255)                                                                                                      cTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGACCTGATATACa                    <  2:271527/150‑1 (MQ=255)                                                                                                         cGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGACCTGATATACATGGt                <  2:315146/151‑1 (MQ=255)                                                                                                                 tAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGACCTGATATACATGGTTCTGCTa         <  2:80702/150‑1 (MQ=255)                                                                                                                  aaTGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGACCTGATATACATGGTTCTGCTaa        <  1:539641/150‑1 (MQ=255)                                                                                                                  aaTGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGACCTGATATACATGGTTCTGCTaa        <  1:103230/150‑1 (MQ=255)                                                                                                                    tGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGACCTGATATACATGGTTCTGCTAACTg     >  1:511332/1‑151 (MQ=255)                                                                                                                         gctgcAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGACCTGATATACATGGTTCTGCTAACTGCtt  <  2:526/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                            CATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAAGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGACCTGATATACATGGTTCTGCTAACTGCTT  >  NC_000913/2703871‑2704139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑