Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,097,218	(G)7→8	intergenic (+389/+347)	ycdU → / ← serX	putative inner membrane protein/tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,097,211	1	.	G	100.0%	12.7 / NA	8	intergenic (+382/+354)	ycdU/serX	putative inner membrane protein/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (2/6);  total (2/6)

GGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCC  >  NC_000913/1097063‑1097303                                                                                                                                                      |                                                                                             ggCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑gg                                                                                            <  1:650576/151‑1 (MQ=33)   cAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑gggg                                                                                          <  2:728921/151‑1 (MQ=33)     gCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGATA‑gggggg                                                                                        >  1:463847/1‑151 (MQ=18)        cGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCCACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTCCTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑ggg                                                                                           <  2:475958/145‑1 (MQ=18)          cTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑gggggggg                                                                                      >  1:228993/1‑147 (MQ=18)            cACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCggggg‑ggggggggggtg                                                                                  >  1:514235/1‑145 (MQ=255)                gCGTTCTCGGTCTGTCCATCTGGTTGTCGCCAGTTGTCGACCCCAGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGAc                                                                                 <  2:791942/148‑1 (MQ=11)                gCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCAGGTGGGGGCGTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtcg                                                                              <  2:481753/151‑1 (MQ=255)                 cGTTCACGGCCTGCCCATCTGGTTGTCGACAGTTGCCCACCCCAGCTGGGGGGTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑gggggggg                                                                                      <  2:45798/141‑2 (MQ=11)                    tCACGGTCTGTCCAACTGGCTGTCGACAGTTCTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtcgctt                                                                           <  2:1057681/150‑1 (MQ=255)                     cACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑gggggggggatgaacc                                                                              >  1:131458/1‑136 (MQ=16)                        ggTCTGTCCAACTGGCTGACGCGAGTTGTCGGCCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTtcgctc                                                                      <  1:727226/151‑1 (MQ=255)                        ggTCTATCCAACTGGCGGTCGCCAGATGCCGCCCCCCGGTCGGGTTGTCTCGTCCACCCGGTTTGTGCAATACAAGAAAAAAAAGCCCGTACTTTCGGACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtcgcttcg                                                                         <  2:421408/148‑1 (MQ=11)                                              cAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCAc                                                <  1:692980/151‑1 (MQ=18)                                                                                          gCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTGAACCCCCCCCCTCGCCCCGCGGGCAGCCCCCCCACTGCGTTCCCGGTCTGTCCCACTCGGCGTCGCCCCTTTTCGAAcc    >  1:434995/1‑151 (MQ=2)                                                                                            aaTACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGAATCCCTTCGCCCCCCCCGCGGGCAGCCCCCTCCCTGTGTTCACGGTCTTTCCAACTGGGTGTCCCCCGTTGTCGGAcccc  >  1:207984/1‑151 (MQ=11)                                                                                                                                                      |                                                                                             GGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCC  >  NC_000913/1097063‑1097303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑