Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 73,581 | 0 | A | C | 43.1% | 92.6 / 17.7 | 58 | V314G (GTA→GGA) | thiP | thiamine/thiamine pyrophosphate ABC transporter permease |
Reads supporting (aligned to +/- strand): ref base A (27/6); new base C (0/25); total (27/31) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.74e-11 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAA‑TACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTC > NC_000913/73432‑73730 | tCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAA‑TACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTa < 1:956509/150‑1 (MQ=255) tCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAA‑TACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAc > 1:61295/1‑151 (MQ=255) tGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAA‑TACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAccc > 1:842131/1‑151 (MQ=255) gcgcCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAA‑TACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATc > 1:23047/1‑151 (MQ=255) cccGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTGCGCACAATACACCTGCCGCCAGCGCAA‑TACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATcgac < 2:595660/151‑1 (MQ=255) gTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAA‑TACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCAc > 1:325565/1‑151 (MQ=255) gTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAA‑TACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCAc > 2:732186/1‑151 (MQ=255) gACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAA‑TACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAg < 2:493729/151‑1 (MQ=255) agcagcATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAA‑TACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGgc > 1:594311/1‑150 (MQ=255) atcatGTCCAGCACCCCCCACAATCCCCCCGCCGCC‑GCCCAATTCCGAAAGGAGGTCCACCGCCCCTCCCACACCCCCGTTGGCGCCGCCACTCCCGCCAACTGGCGATTTACCCCACCGACGACCCCCGCCAGTAACGGTGGCAGCaa < 2:919931/149‑1 (MQ=255) atcatGGCCAGCCCTCCGCACAACCCCCCGGCCGCCAGCGCAA‑TACGAAACGGGCCCCACAGCCCCTCCCACAGCCCCGGTTGTGCCAGCCCTCCCGGAACCGGGCGATTTCCCCCATCGCCAATCACCGCCAGTAACGGTGgcagca < 2:986151/148‑1 (MQ=255) tcatGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAA‑TACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAAc > 1:17233/1‑149 (MQ=255) tACGCACAATACACCTGCCGCCAGCGCAA‑TACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGc > 2:28803/1‑151 (MQ=255) aCGCACAATACACCTGCCGCCAGCGCAA‑TACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGc > 2:281768/1‑150 (MQ=255) cacaATACACCTGCCGCCAGCGCAA‑TACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGcaca > 2:553681/1‑150 (MQ=255) aacacccccccccccAGCACAA‑TACACAACGAGCCCCACAGCGCCGCCCACAGCCCCGGTTGTGCCGGCTCTTCCGGCAACTGGCGATTTCCCCCACCGACAATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAAtt < 2:628786/137‑1 (MQ=255) aaTCCACCTGCCGCAAGCGCAA‑TCCCCACCGAGCCCCACGCCCCCGGCCACACCACCGGTTGGGCCAGCATTTCCGGAAACGGGCGTTTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAAtt < 1:28268/150‑1 (MQ=255) tACACCTGCCGCCAGCGCAA‑TACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTa > 2:720444/1‑149 (MQ=255) tACACCTGCCGCCAGCGCAA‑TACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAac > 2:626074/1‑151 (MQ=255) acacCTGCCGCCAGCGCAA‑TACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAac > 1:897761/1‑150 (MQ=255) cccccccccccAGCGCAA‑TACGCAACGGGCCCCACCGCGCCTGCCACAGCCCGGGTTGGGCCAGCACTTCCGGCAACGGGCGTTTTCCCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAac < 2:459466/140‑1 (MQ=255) cgccAGCGCAA‑TACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCg > 1:663953/1‑151 (MQ=255) gccAGCGCAA‑TACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGCGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGc > 2:493051/1‑151 (MQ=255) cgccaggcaaaaacccAACGGGGCCCACACCCCCTCCCACAGCACCGTTTGTGCCGCCACTCCCGGAACCGGGCTATTTCCCCCACCGACAATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCg < 2:1026845/136‑1 (MQ=255) ccggcgcAA‑ACCACAACGGGCCCCACCGCCCCCCCCACACCCCGGGTTGTGCCGCCACTCCCGGCAACGGGCGATTTCCCCCACCGCCGATCCCCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACAcc < 2:686648/140‑1 (MQ=255) gcgcAA‑TCCCCAACGAGCCCCACCGCCCCCCCCACGCCCCCGGTTGGCCCAGCACTTCCGGCACCGGGGGTTTTCCCCCATCGACATTCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCaa < 2:244284/149‑1 (MQ=255) gcaaaccGCAACGGGGTCCAAAGCCCCTGCCACACCACCGGTGGTGCCAGCCCTCCCGGCAACTGGGGATTTCCCCCATCGACGAACACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCaaa < 1:997449/142‑1 (MQ=255) aa‑TACGCAACGAGGCCCACAGCGCCTGCCACAGCCCGGGTGGGGCCAGCACTCCCGGCAACGGGCGATTTCCCCCACCGCCGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGc < 1:169098/149‑1 (MQ=255) ccGCACCGAGGCCCACGCCGCCTGCCACAGCCCCGGTTGGCCCAGCCCTCCCGGCATCTGGCGTTTTCCCCCATCGACGATCTCCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCt < 1:659665/149‑1 (MQ=255) cAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGc > 2:465176/1‑151 (MQ=255) ggggcccacgcccccTCCCACAGCCCCGGTTGGGCCAGCACTCCCGGCAACGGGCGATTCCCCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGa < 2:247557/137‑1 (MQ=255) ggcccAGAGCGCCTGCCACAGCCCGGTTTGCGCCAGCACTTCCGGCAACGGGCGATTTCCCCCACCGACGTTCTCCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGa < 2:194807/148‑1 (MQ=255) ccccggccccTGCCACAGCCCGGTTTGTGCCAGCACTTCCGACAGTTGGCGTTTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGa < 2:963839/140‑1 (MQ=255) ccACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCg > 1:574888/1‑151 (MQ=255) cggcgcCTCCCACAGCACCGGTTGGGCCAGCCCTCCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTcc < 1:567293/149‑1 (MQ=255) gccccTGCCACAGCACCGGTTGGGCCAGCTCTCCCGGCAACTGGCGATTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCg < 1:1061638/147‑1 (MQ=255) cgcCTGCCACAGCCCCGGTTGCGCCAGCCCTCCCGGCAACTGGAGATTTCCCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCg < 2:891717/149‑1 (MQ=255) gcCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGt > 1:608890/1‑151 (MQ=255) cTGCCCCAGCCCCGTTTGTGCCAGCACTTCCGGCAACTGGCGATTCCCCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCggg < 1:908124/148‑1 (MQ=255) ccACAGCCCGGTTTGCGCCAGCATTCCCGGCAACGGGCGATTTCCCCCAGCGACGAACACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTcc < 2:33313/142‑1 (MQ=255) cccgggtggggCCACCACTCCCGGCACGGGGGGATTTCCCCCATCGACGACCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGGTATGCAGACGATCGTCCGGGTCGCGCCa < 1:161922/137‑1 (MQ=255) aCCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCtt > 2:232904/1‑151 (MQ=255) tgcgCCAGCCCTCCCGCCAACTGGCGTTTTCCCCCATCGCCGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGc < 1:664789/139‑1 (MQ=255) tgCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCagcagc > 1:494403/1‑151 (MQ=255) gCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGCGGCAGCAACAGCAGCAGCGCCAGCACACTTAACACCGGGTCGCAAATGCGACTAAGCAGACGATCGTCCGGGTCGCGCCAGCCTTgcagcaac > 2:445103/1‑148 (MQ=255) ccaccccTTCCGGCAACTGGGATTTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTgcagca < 1:858667/141‑1 (MQ=255) cAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCgtg > 2:136526/1‑151 (MQ=255) aGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCgtgg > 1:373419/1‑151 (MQ=255) gCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCgtggt > 1:58265/1‑151 (MQ=255) cTTCCGGAAACTGGCGATTTCCCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCgtg < 1:661213/146‑1 (MQ=255) tCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCgg > 2:1002135/1‑151 (MQ=255) ccGGCACCTGGCGATTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCggg < 1:980578/151‑1 (MQ=255) ggCAACTGGCGATTTCCCCCATCGACGACCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCggg < 2:663935/149‑1 (MQ=255) ggCAACGGGCGATTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGgc < 2:494389/150‑1 (MQ=255) ggaccgggCGATTTCCCCCTTCGACAATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGcc < 1:748701/139‑1 (MQ=255) ggCGTTTTCCCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGc < 1:606893/151‑1 (MQ=255) gCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGcc > 1:800779/1‑151 (MQ=255) aTTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCtt > 1:183964/1‑150 (MQ=255) tttACCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATgg < 1:1054363/145‑1 (MQ=255) aCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTc > 1:101419/1‑150 (MQ=255) | TCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAA‑TACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTC > NC_000913/73432‑73730 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |