Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,288	0	T	G	41.7%	115.8 / 11.3	61	V528G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (3/32);  new base G (25/0);  total (29/32)
Fisher's exact test for biased strand distribution p-value = 6.31e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGG  >  NC_000913/354148‑354434                                                                                                                                             |                                                                                                                                                    ccGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGG‑GAAGAt                                                                                                                                             >  1:615485/1‑148 (MQ=255)  cGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGG‑AAAGAAgcg                                                                                                                                          >  2:1026729/1‑150 (MQ=255)         cTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGG‑GAAGATGCGCTggcagg                                                                                                                                  >  2:926330/1‑146 (MQ=255)           gACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAggg                                                                                                                                 <  1:723968/150‑1 (MQ=255)            aCGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTCGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGTCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGc                                                                                                                                <  2:382173/150‑1 (MQ=255)              ggTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGCAgg                                                                                                                             <  2:957693/151‑1 (MQ=255)              ggTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGG‑AAAGATGCGCTGAAAGGGCAgg                                                                                                                             >  1:684777/1‑151 (MQ=255)                   tCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGTAAGGATG‑GCTGAAAGGGCAgggggcg                                                                                                                        >  2:646624/1‑151 (MQ=255)                      cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGCAggtggcggt                                                                                                                      <  1:177210/150‑1 (MQ=255)                      cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGCAggtggcggt                                                                                                                      <  1:96401/150‑1 (MQ=255)                        ttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGG‑GAAGAAGCGCTGGAAGGGGAgggggcgggggg                                                                                                                   >  2:1009676/1‑150 (MQ=255)                         tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGCAggtggcggtggc                                                                                                                   <  1:184864/150‑1 (MQ=255)                         tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGCAggtggcggtggc                                                                                                                   <  2:1029922/150‑1 (MQ=255)                              tcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGG‑AAAGAAGCGCTGGAAGGGCAgggggcggtgggggtt                                                                                                               >  2:771470/1‑144 (MQ=255)                               cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTg                                                                                                              <  1:878986/149‑1 (MQ=255)                               cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTg                                                                                                              <  2:2797/149‑1 (MQ=255)                               cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTg                                                                                                              <  1:326782/149‑1 (MQ=255)                                  gcgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCAtt                                                                                                         <  2:632020/151‑1 (MQ=255)                                     cACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTcct                                                                                                      <  2:405168/151‑2 (MQ=255)                                     cACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCg                                                                                                      <  2:581913/151‑1 (MQ=255)                                         gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGG‑GAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGGTTGTCATTCCGaa                                                                                                    >  1:319739/1‑149 (MQ=255)                                          aCGATGTAATTAACGTTGCCGGGCATCGGATGGGGACGCGCGAGAGTGAAGAGAGTATCTACAGCGATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGaaa                                                                                                   <  2:955915/149‑1 (MQ=255)                                          aCGATGTAATTAACGTTGCCGGGCACCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTGCAGTGATCCGGGCGTTGGCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGGGGAAAGAGGAGGTGGCGGTGGCGTTTGTCATTCCGag                                                                                                    <  2:848125/148‑2 (MQ=255)                                           cGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAagag                                                                                                <  1:10592/151‑1 (MQ=255)                                             aTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGG‑GAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTTTCATTCCGAAaga                                                                                                 >  2:287372/1‑148 (MQ=255)                                              tGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGG‑GAAGAAGCGCTGGAAGGGGAGGGGGGGGGGGGGTTTGTCTTTTCGGAagagag                                                                                              >  2:153183/1‑150 (MQ=255)                                               gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGG‑AAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGTTTGTCATTTCGAAAGAGAGc                                                                                             >  1:408397/1‑150 (MQ=255)                                               gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGG‑AAAAATGCGCTGAAAGGGCAGGTGGGGGGGGGGTTTGTCATTCCGAAagag                                                                                                >  1:306300/1‑147 (MQ=255)                                                    tAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAg                                                                                        <  2:1005012/150‑1 (MQ=255)                                                          tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGG‑AAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGTTTGTCATTTCGAAAGAGAGCGACAGTCTgg                                                                                   >  1:565076/1‑149 (MQ=255)                                                           gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGaa                                                                                 <  1:7043/150‑1 (MQ=255)                                                                gCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGt                                                                           <  2:227451/151‑1 (MQ=255)                                                                 cATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTTGGGGG‑AAAAAAGCGCCGGAAGGGCAGGTGGCGGGGGCGGTTTTTCTTCCGAAAGAGAGCGACAGTCTGGAAGATCGTg                                                                          >  1:481089/1‑151 (MQ=255)                                                                     ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGG‑AAAGAAGCGCTGGAAGGGCAGGGGGCGGGGGCGGTTGTCATTCCGCAAGAGAGGGAAAGTCTGGAAGaaagcg                                                                          >  2:726506/1‑142 (MQ=255)                                                                        tGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGG‑AAAGATGCGCTGAAAGGGCAGGGGGGGGGGGGGTTTGTCATTTCGAAAGAGAGGGACAGTCTGGAAGATCGGGGTGTgg                                                                    >  1:73230/1‑150 (MQ=255)                                                                         ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTgg                                                                    <  2:632713/149‑1 (MQ=255)                                                                         ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGG‑GAAAAAGCCCTGAAAGGGGCGGGGGGGGGGGCGGTTGTCATTCTGAAAGAGAGCGACAGGCTTGAAGAGCGTGAagt                                                                      >  2:187386/1‑147 (MQ=255)                                                                           ggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGG‑AAAGATGCGCTGAAAGGGCAGGGGGGGGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGGCTGGAAGATCGTGATGTGgcg                                                                  >  1:699014/1‑149 (MQ=255)                                                                           ggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGAG‑GAAGACGCGCTGAAAGGGCAGGGGGAGGCGGCGGTTGTGTTTCCGCAAGAGAGCGAGAGGCTGGGAGATCGTGCAGTGgggc                                                                 >  2:129151/1‑150 (MQ=255)                                                                              cgcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTc                                                             <  1:1044955/151‑1 (MQ=255)                                                                                cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGG‑GAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTTTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCg                                                            >  2:263940/1‑150 (MQ=255)                                                                                    agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAAGGCAGGTGGCGGTGGCGTTTGTCATTTCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCa                                                          >  1:457325/1‑148 (MQ=255)                                                                                    agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGG‑GAAGATGCGCTGGAAGGGCAGGTGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTc                                                             >  2:428110/1‑145 (MQ=255)                                                                                     gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAagag                                                      <  1:211686/151‑1 (MQ=255)                                                                                         gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAgg                                                  <  1:887973/151‑1 (MQ=255)                                                                                           agagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGG‑GAAGATGCGCTGAAAGGGCAGGGGGGGGGGGGGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCg                                                >  1:520172/1‑151 (MQ=255)                                                                                               agTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGG‑TAAGATGCGCTGAAAGGGCAGGTGGGGGTGGCGTTTGTCATTCCGAAAGAGAGCGGCAGTCTGGAAGATCGGGGTGTGGCGCACTCGCAAGAGAAGGCGAtt                                             >  2:239914/1‑150 (MQ=255)                                                                                                  aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATg                                          <  1:365216/150‑1 (MQ=255)                                                                                                    ctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGG‑GAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGATGTGGCGCACTCGCAAGAGAAGGCGATTATgg                                         >  1:671251/1‑149 (MQ=255)                                                                                                       cAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGG‑AAAAATGCCCCGAAAGGGGAGGGGGCGGGGGCGGTTGTTATTCCGAAAAAGAGGGGCAGTCTGGGAGAACGGGGGGTGGGGGCCCCGCAAAAGGAAGGGGTTATGGGGCt                                     >  2:316431/1‑150 (MQ=255)                                                                                                         gCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggt                                  <  2:348144/151‑1 (MQ=255)                                                                                                           cATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggtgg                                <  2:469149/151‑1 (MQ=255)                                                                                                              ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGAc                              <  2:652102/150‑1 (MQ=255)                                                                                                              ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACa                             <  1:617796/151‑1 (MQ=255)                                                                                                                      tGCCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGAt                      <  2:1027139/150‑1 (MQ=255)                                                                                                                       gCCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGAtt                     <  2:91138/150‑1 (MQ=255)                                                                                                                         cGAAGTGGCGGTGGTTGGGGGG‑GAAAATGGGGTGAAAGGGCAGGGGGCGGGGGCGTTTGTCATTTCGAAAGAGAGCGACAGTCTGGAAGATCGAGATGTGGGGCACTCGCCAGAGAAAGCGAATATGGGGCTGGGGGAAAGCCAGATTgg                   >  2:719232/1‑150 (MQ=255)                                                                                                                          gAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCa                 <  1:855459/151‑1 (MQ=255)                                                                                                                                 cGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTgg          >  2:40156/1‑151 (MQ=255)                                                                                                                                     ggtTGGGGTG‑AAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccg       <  2:684758/150‑1 (MQ=255)                                                                                                                                         ggggTG‑AAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCgg  >  1:896477/1‑151 (MQ=255)                                                                                                                                             |                                                                                                                                                    CCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTG‑AAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGG  >  NC_000913/354148‑354434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑