Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913504,6400CT43.8% 59.7 / 32.7 65K19K (AAG→AAAfsrputative fosmidomycin efflux system protein
Reads supporting (aligned to +/- strand):  ref base C (28/8);  new base T (0/28);  total (28/37)
Fisher's exact test for biased strand distribution p-value = 4.82e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGTTATCATGCCAATCTGCATAAATGTCAGAGAAAATTCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAG  >  NC_000913/504498‑504786
                                                                                                                                               |                                                                                                                                                    
gggTTATCATGCCAATCTGCATAAATGTCAGAGAAAATTCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGt                                                                                                                                            <  2:615384/151‑1 (MQ=255)
gggTTATCATGCCAATCTGCATAAATGTCAGAGAAAATTCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGt                                                                                                                                            <  1:779122/151‑1 (MQ=255)
   ttATCATGCCAATCTGCATAAATGTCAGAGAAAATTCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTg                                                                                                                                          >  2:471001/1‑150 (MQ=255)
   ttATCATGCCAATCTGCATAAATGTCAGAGAAAATTCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTg                                                                                                                                          >  1:240579/1‑150 (MQ=255)
   ttATCATGCCAATCTGCATAAATGTCAGAGAAAATTCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTg                                                                                                                                          >  2:802981/1‑150 (MQ=255)
           ccAATCTGCATAAATGTCAGAGAAAATTCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCcg                                                                                                                                 >  1:376776/1‑151 (MQ=255)
              aTCTGCATAAATGTCAGAGAAAATTCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCgcgc                                                                                                                              >  2:618333/1‑151 (MQ=255)
               tCTGCATAAATGTCAGAGAAAATTCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGcgcc                                                                                                                             >  2:961068/1‑151 (MQ=255)
                   cATAAATGTCAGAGAAAATTCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGcgcccgcc                                                                                                                         >  2:108711/1‑151 (MQ=255)
                    aTAAATGTCAGAGAAAATTCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGcgcccgcc                                                                                                                         >  1:226574/1‑150 (MQ=255)
                                gaAAATTCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGgtt                                                                                                            >  1:695378/1‑151 (MQ=255)
                                 aaaaTTCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGgttg                                                                                                           >  1:306157/1‑151 (MQ=255)
                                     ttCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTc                                                                                                        >  1:590774/1‑150 (MQ=255)
                                      tCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCAc                                                                                                      >  2:840074/1‑151 (MQ=255)
                                      tCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCAc                                                                                                      >  2:855795/1‑151 (MQ=255)
                                          actgaAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCAt                                                                                                  >  1:874779/1‑151 (MQ=255)
                                          acggaAGCAGCGAATAAATCGCAAGAAGCGGCTATTGGTTCATGTCGTTCAGCAGATGTGAGGGGGTAATAGCACCAAAAACACCAAACGATGTGCGGGTTTTGGTCGTGGACCCGGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCAt                                                                                                  <  2:1043705/148‑1 (MQ=255)
                                           cggaAGCGGCGGATAAATCGCAAGAATCAGCGTTTGGATCATGTCGTCCGGCAGATGTGGGGGGCTGAAAGCACCTAAAATCCCAAACGATGTGGGGTCTTTGGTCGTTGACGCGGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCAtt                                                                                                 <  2:188111/149‑1 (MQ=255)
                                              agggggggaaaaaaCCGCCAAAATCAGCGTTTGGATCAGGTCTTTCGGCAGTTGTGGGGGGTTGATAGCCCAAAAAATCCAAACGGTTGTGGGGCCTTTGGTCGTTGCCGCGGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTg                                                                                                <  2:404105/138‑1 (MQ=255)
                                              aaccaggGGATAAACCGCAAAAATCAGCGTTTGGATCATGTCGTTAAGAAGATGTGAGAGGCTGATACCCCTAAAAATCCCAAACGAGGTTCGGTCTTTGGTCTTTGCCGCCGCCGCCCCCGCCACAGGCTGGGGTTGTTCACTCAtt                                                                                                 <  1:553581/145‑1 (MQ=255)
                                                   gcGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAgg                                                                                         >  2:672342/1‑151 (MQ=255)
                                                   gcGGAAAAACCGCAAGAATCAGCGATTGGATCATGTCGTCCAGCAGATGTGAGGGGCTGATAGCCCCTAAAATCCCAAACGATGTTGGGGCTTTGTTCGTGGCCGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCa                                                                                             <  1:413472/147‑1 (MQ=255)
                                                       aaaaaCCGCCAAAATCAGCGATTGAATCATGTCGTCCAGCAGATGGGAGAGGCTGATACCACAAAAAAACCAAAACGTTGTGGGGGCTTTGGTCGTGGACGCGGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGaa                                                                                       <  1:711310/147‑1 (MQ=255)
                                                         aaaCCGCGAGAATCAGCGATTGGATCATGTCGTCCAGCAGAGGGGAGGGGCTGAAACCACAAAAAATCCAAAACGTTTTTGGGGCTTGGTTGGTGGACGCCGCCGCACCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTc                                                                                   <  1:868966/151‑1 (MQ=255)
                                                          aaTCGCAAAAACCAGCGATTGGATCATGTCGTTCAGCAGTGGGGAGGGGCTAAAAGCACCAAAAATCCAAAACGTTTTGGGGTCTTTGGGCATTAGCCCGCCCCCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCa                                                                                  <  1:443250/151‑1 (MQ=255)
                                                           aTCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCAc                                                                                 >  1:717395/1‑151 (MQ=255)
                                                                 aaaaTCAGGGTTGGGATCTTGTCGTGCAGAAGGTGGGGGGGGCTGATACCACCAAAAATACAAAACGTTTTTGGGGTTTGGTTCTTGGACGCGGCCCCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACttttt                                                                            <  2:870041/148‑1 (MQ=255)
                                                                 aGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTc                                                                           >  1:778761/1‑151 (MQ=255)
                                                                 aGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTc                                                                           >  2:84383/1‑151 (MQ=255)
                                                                 aGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCAAAAATCCCAAACGATGTTGGGGCTTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACtttt                                                                             <  1:742458/149‑1 (MQ=255)
                                                                 aGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCAAAAATCCAAAACGATGTTCGGGCTTTGGTGGTGGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACtttt                                                                             <  1:599661/149‑1 (MQ=255)
                                                                       agtgattgattCATGTCGTTCGGCAGGTGGGGGGGGCTGATACCCCAAAAAAACCCAAACGGTGTGGGGGTTTTGTTCGTTGCCGCGCCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAggg                                                                       <  2:594006/146‑1 (MQ=255)
                                                                          gATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCg                                                                  >  2:233243/1‑151 (MQ=255)
                                                                            ttGGATCATGTCGTTCAGCAGATGGGAGAGGCTAATAGCCCCAAAAATCCAAAACGTTTTTCGGGCTTTGGTTTTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCg                                                                  <  2:902531/149‑1 (MQ=255)
                                                                            ttGGATCATGTCGTTCAGCAGAGGTGAGAGGCTGATAGCACCAAAAATCCCAAACGATGTTGGGGCTTTGGTCTTTGACGCAGCCGCCCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGc                                                                   <  1:897670/148‑1 (MQ=255)
                                                                              ggATCATGTCGTTCAGCAGATGTGAGAGGCTGAGAGCCCCTAAAATACCAAACGATGTTGGGGCCTTGGTCGTTGACGCGGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGAt                                                                <  1:698880/149‑1 (MQ=255)
                                                                              gaataatttCGTTCAGAAGATGGGAGGGGCTGAACGCACCAAAAATACCAAGCGTGTTTCGGTCTTGGTTTTTTGCCGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATg                                                               <  2:663647/142‑1 (MQ=255)
                                                                                 tCATTTCGTTCAGCAGATGTGAGGGGTGGATACCCCTAAAAACACAAAACGGTTTTCGGCCTTTGTTCGTTGCCGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTaa                                                            <  2:394381/150‑1 (MQ=255)
                                                                                     gtggtgcagcagTTGGGGGAGGATAAAACCACAAAAAATACAAACCGATGTGGGGGCTTGGGTGGTTGACGCACCGGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTaa                                                            <  1:372059/140‑1 (MQ=255)
                                                                                         ttCAGCAGAGGTGAGGGGCTGATAGCACCAAAAATACCAAACGATGTTCGGGCTTTGGTCGTGGACGCGGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTaaa                                                           <  1:743812/143‑1 (MQ=255)
                                                                                         tacagcagGTGTGAGAGGCTTATACCCCCAAAAACCCAAAACTATGTTGGGCCTTTTGTCTTTGACGCACCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATg                                                      <  2:923785/146‑1 (MQ=255)
                                                                                              cagATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTAtt                                              >  2:870151/1‑151 (MQ=255)
                                                                                                gATGTGAGTGGCTGATACCCCTAAAAATACCAAAGGTTGTGGGGCCCTTGGTCGCTGACGCCGCGGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCtt                                                 <  1:130220/146‑1 (MQ=255)
                                                                                                   gtggggggCTGATAGCCCAAAAAATACAAAACGTTGTTGGGCCTTTGGTCGTTGACGCGGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTAttt                                             <  1:725394/141‑1 (MQ=255)
                                                                                                   gggagaGGCTGATAGCACCTAAAATACCAAACGATGTGGGGGCCTTGGTCGTTGACGCGGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCtt                                                 <  1:1006379/141‑1 (MQ=255)
                                                                                                   gggagaGGCTAACAGCACCTAATCACCAAATCGATGGGGGGGCTTTGATAGTTGACACAGCGACCCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTGAtt                                              <  2:203684/144‑1 (MQ=255)
                                                                                                     gagaGGCTGATAGCACCAAAAATACCAAACGATGTTGGGGCTTTGGTCGTTGACGCGGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGa                                         <  1:86593/149‑1 (MQ=255)
                                                                                                       gagGCGGAACGCACTAAAAATCCCAACCGTTGTTCGGGCTTTGGTGGTTGACGCGGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGatta                                      <  1:362203/150‑1 (MQ=255)
                                                                                                          gCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTAc                                  >  2:854630/1‑151 (MQ=255)
                                                                                                           cTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTAcc                                 >  2:644154/1‑151 (MQ=255)
                                                                                                              aTAGCACCAAAAATACCAAACGATGTTGGGGCTTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTAcc                                 <  1:672360/148‑1 (MQ=255)
                                                                                                                aGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCACCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACAAGACt                            >  2:503909/1‑151 (MQ=255)
                                                                                                                     cTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACa                        >  1:391724/1‑150 (MQ=255)
                                                                                                                       aaaaTCCCAAACGATGTTGGGGCTTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACAt                       <  1:79227/149‑1 (MQ=255)
                                                                                                                          aTACCAAACGATGTTCAGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTg                  >  1:56411/1‑151 (MQ=255)
                                                                                                                            aacAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGACACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTg                  >  2:1062596/3‑149 (MQ=255)
                                                                                                                             ccAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTAt               >  1:700536/1‑151 (MQ=255)
                                                                                                                               aaaCGTTTTTCGGGCGTTGGTCTTTGCCGCACCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGt                 <  1:991719/147‑1 (MQ=255)
                                                                                                                               aaaCGTTGGTGGGGTTTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGc             <  2:1018861/151‑1 (MQ=255)
                                                                                                                               aaaCGATGTGGGGGCTTTGTTCGTTGCCGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGc             <  2:231630/151‑1 (MQ=255)
                                                                                                                                aaagggtgtgggggCTTGGGTGGTGGCCGCGGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGt                 <  2:201219/136‑1 (MQ=255)
                                                                                                                                 aCGTTGTTCGGGCCTTGGTCGTGGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGcgt           <  1:837190/151‑1 (MQ=255)
                                                                                                                                 aCGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGcgt           >  1:506150/1‑151 (MQ=255)
                                                                                                                                        tgcGGGCTTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCt      <  1:1018679/148‑1 (MQ=255)
                                                                                                                                           gggCTTTGGTCGTGGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTgaag  <  1:318951/151‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
GGGTTATCATGCCAATCTGCATAAATGTCAGAGAAAATTCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAG  >  NC_000913/504498‑504786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: