Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	646,747	0	T	G	45.5%	99.4 / 10.3	66	T255P (ACC→CCC)	citG	2‑(5''‑triphosphoribosyl)‑3'‑dephosphocoenzyme‑A synthase
Reads supporting (aligned to +/- strand):  ref base T (8/28);  new base G (30/0);  total (38/28)
Fisher's exact test for biased strand distribution p-value = 1.40e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAA‑T‑G‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCC  >  NC_000913/646600‑646897                                                                                                                                                    |                                                                                                                                                            atatTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑g                                                                                                                                                         <  2:587285/151‑1 (MQ=255)   atTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑Gaa                                                                                                                                                       <  2:980401/151‑1 (MQ=255)   atTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑Gaa                                                                                                                                                       <  1:782220/151‑1 (MQ=255)     ttCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAA‑t                                                                                                                                                     <  1:576444/150‑1 (MQ=255)      tCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑Gaa                                                                                                                                                       >  2:637306/1‑148 (MQ=255)          aaTTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTC‑GGA‑T‑G‑ccccc                                                                                                                                             >  2:304390/1‑151 (MQ=255)          aaTTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTC‑GAA‑T‑G‑ccccc                                                                                                                                             >  1:635176/1‑151 (MQ=255)               aTCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAA‑T‑G‑CCCCCtttt                                                                                                                                         <  1:149576/150‑1 (MQ=255)                 cAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTT‑GAA‑T‑G‑GCCCCctttt                                                                                                                                        >  1:669735/1‑149 (MQ=255)                  aaGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAA‑T‑G‑CCCCCTTTTTGCa                                                                                                                                     <  1:1000042/151‑1 (MQ=255)                   aGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTC‑GAA‑T‑G‑CCCCCctttt                                                                                                                                        >  1:930997/1‑147 (MQ=255)                    gTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAA‑T‑G‑CCCCCTTTTTGCaat                                                                                                                                   <  2:698266/151‑1 (MQ=255)                      gCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTC‑GCA‑T‑T‑CCCCCCTTTTTCtata                                                                                                                                  >  1:752246/1‑150 (MQ=255)                         tAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTTGTCTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCTTCGGGGGGTC‑GAA‑T‑G‑cgccccctttcgaaga                                                                                                                                  >  2:181745/1‑136 (MQ=255)                         tAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAA‑T‑G‑CCCCCTTTTTGCaataat                                                                                                                                <  1:747746/149‑1 (MQ=255)                          aaaTAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAA‑T‑G‑CCCCCTTTTTGCAATAATGtt                                                                                                                             <  2:883852/151‑1 (MQ=255)                          aaaTAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAA‑T‑G‑CCCCCTTTTTGCAATAATGt                                                                                                                              <  1:472574/150‑1 (MQ=255)                           aataatTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTT‑GAA‑T‑G‑GCCCCCTTTTTTTATAATGttt                                                                                                                            >  1:311563/1‑151 (MQ=255)                              aatTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTC‑GAA‑A‑G‑GCCCCCTTTTTCaataaatttt                                                                                                                            >  1:15683/1‑143 (MQ=255)                               atTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTG‑GGA‑A‑G‑CCCCCTTTTTTCCATTATATTTtcgc                                                                                                                        >  1:195734/1‑151 (MQ=255)                                 tAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAA‑T‑G‑CCCCCTTTTTGCAATAATGTTTGCGcc                                                                                                                       <  2:970534/150‑1 (MQ=255)                                 tAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTT‑GGA‑A‑G‑CCCCCTTTTTTtaataaaattt                                                                                                                            >  1:258674/1‑140 (MQ=255)                                   aaTCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTT‑GGA‑A‑G‑ACCCCTTTTTTCaaatatatttttgcccc                                                                                                                     >  1:34873/1‑135 (MQ=255)                                       tgtgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAA‑T‑G‑GCCCCTTTTTTCAAAAATGTTTGGGCCCcgcg                                                                                                                  >  2:111569/1‑149 (MQ=255)                                       tgtgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAA‑A‑G‑CCCCCCTTTTGTAATAAATTTTTCGCCTccc                                                                                                                   >  1:364903/1‑146 (MQ=255)                                         tgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTC‑GCA‑T‑G‑CCCCCCTTTTTTAATAATTTTTTGGCCTCGCGCTg                                                                                                               >  2:152729/1‑150 (MQ=255)                                            tAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTT‑GAA‑T‑G‑CCCCCCTTTTTTAAAAAAATTTTTGCCTcgc                                                                                                                   >  2:657881/1‑143 (MQ=255)                                              aaaaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTT‑GAA‑T‑A‑CCCCCCTTTTTCAATAAAGTTTTCGCCCcg                                                                                                                    >  1:590086/1‑140 (MQ=255)                                                aaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTC‑GAA‑T‑G‑CCCCCCTTTTTTAATAATTTTTTTGCCTCCCGCCGTATCCa                                                                                                         >  2:820074/1‑149 (MQ=255)                                                 aCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAA‑T‑G‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAgcg                                                                                                      <  2:540099/151‑1 (MQ=255)                                                 aCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTT‑GAA‑T‑G‑GCCCCCTTTTTTTATAATTTTTTTGCCTCGCGCTggtgcccgc                                                                                                       >  2:208275/1‑142 (MQ=255)                                                   cAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTT‑GGA‑T‑G‑CCCCCCTTTTTTAATAATTTTTTGGCCCCGCGCTGGAg                                                                                                            >  2:671636/1‑143 (MQ=255)                                                   cAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGAA‑G‑C‑CCCCCTTTTTCCATAAATGTTTGGGccc                                                                                                                      >  2:803888/1‑133 (MQ=255)                                                        aaGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTT‑GGA‑T‑G‑CCCCCTTTTTTCCATAATATTTGCGCCCCGCCCTGGCGCCCGCGCAGGccc                                                                                               >  1:524161/1‑151 (MQ=255)                                                              cAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAA‑T‑G‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCCGTAGCCAGCGCAGGCCCCCCTc                                                                                          <  2:722407/150‑1 (MQ=255)                                                               aGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTT‑GAA‑T‑T‑CCCCCTTTTTTTAATAATGTTTTTGCCCCCCGCTGTGGCCAGAGCAGGCCCCCCTc                                                                                          >  1:318188/1‑149 (MQ=255)                                                                  aGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAA‑T‑G‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGccg                                                                                      <  2:11227/150‑2 (MQ=255)                                                                      cAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTC‑GAA‑T‑GCCCCCCTTTTTGC‑ATAATGTTTTCGCCCCGCGCTGGAGGCAACGCAGGCCCCCCCCGCcccc                                                                                    >  1:825/1‑148 (MQ=255)                                                                           cTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTC‑GAA‑A‑G‑CCCCCCTTTTTCAATAATTTTTTCGCCCCGCGCTGTAGCCAGCGCAGGCCCCCCCCCCCAccccccggtg                                                                            >  1:451761/1‑143 (MQ=255)                                                                             gccgccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTC‑GGA‑T‑G‑GCCCCTTTTTTCAATAATTTTTTCGCCTCGCGCCGTAGCCAGCGCCGGCCCCCCCCCCCAcccccc                                                                                >  1:158012/1‑141 (MQ=255)                                                                                 ccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAA‑T‑G‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGAAGGCCCCCCTCGCCTCCGCGCGATGCAA‑c                                                                       <  2:964261/149‑1 (MQ=255)                                                                                  cTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTC‑GAA‑T‑G‑CCCCCTTTTTTTAATAATGTTTTCGCCCCGCGCCGTTGCCAGCGCAGGCCCCCCCCCCCCCCGCGCGATGCAA‑Cggt                                                                    >  1:37401/1‑149 (MQ=255)                                                                                         gagaTTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAA‑T‑G‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCg                                                             <  1:625254/151‑1 (MQ=255)                                                                                         gagaTTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAA‑T‑G‑CCCCCCTTTTTCAATAATGTTTGTGCCCCCCGCTGTAGCCAGCCCCGGCCCCCCCCCCCACCCCCCGGTGCAA‑Cgct                                                                    >  2:797217/1‑142 (MQ=255)                                                                                          agaTTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAA‑T‑G‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGc                                                            <  1:1049326/151‑1 (MQ=255)                                                                                            aTTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTC‑GGA‑T‑G‑CCCCCCTTTTTCAAAAATTTTTTCGCCCCGCGCTGTAGCCAGCGCCGGCCCCCCCCCCCCCCCCCCGGTGCCA‑CGTTGGTGTCGcc                                                           >  1:419705/1‑150 (MQ=255)                                                                                               tcgttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTT‑GGA‑T‑G‑CCCCCCTTTTTCAATAAATTTTTTGCCCCCCGCCGTAGCCAGCGCCGGCCCCCCCCCCCCCCCCCCGCTGCAA‑CGTTGGTGTTGcc                                                           >  2:579758/1‑147 (MQ=255)                                                                                                cgttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAA‑T‑G‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGa                                                      <  1:1047495/151‑1 (MQ=255)                                                                                                  ttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTC‑GAA‑T‑G‑CCCCCTTTTTTCAATAATGTTTGCCCCTCCCGCTGTAGCCAGCGCACGCCCCCCCCCCCCCCCCGCGATGCAA‑CAGTGGGATCGcccgtggtc                                                    >  1:745541/1‑144 (MQ=255)                                                                                                    cgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAA‑T‑G‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCg                                                   <  2:889763/150‑1 (MQ=255)                                                                                                    cgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAA‑T‑G‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGc                                                  <  2:126736/151‑1 (MQ=255)                                                                                                     gATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAA‑T‑G‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGcc                                                 <  1:374223/151‑1 (MQ=255)                                                                                                     gATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTC‑GAA‑T‑G‑ACCCCCTTTTGTCATAAATTTTGTGCCCCCCGCTGGAGCCAGCGCCGGCCCCCCTCCCCCCCCCGCGGTGCAA‑CGTTGGTATTGACGGTGATTGcc                                                 >  1:219938/1‑151 (MQ=255)                                                                                                        acacTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAA‑T‑G‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCAt                                               <  1:168007/150‑1 (MQ=255)                                                                                                                 gTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAA‑T‑G‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCATCAGTAGGAg                                      <  1:458157/150‑1 (MQ=255)                                                                                                                   ctaaCTGCCGGAGATAATCGAGATCGGCGGGGGGTC‑GAA‑A‑G‑CCCCCCTTTTTCAATAAAGTTTTCGCCTCCCGCTGGAGCCAGCGCAGGCCCCCCCCCCCCCCCCCCGATGGAA‑AGTTGGTATTGCCGGTGGTCGCCCTCAGTAGGAg                                      >  1:210795/3‑148 (MQ=255)                                                                                                                   cGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GGA‑T‑G‑CCCCCCTTTTGCAATAATGTTTTCGCCCCCCGCTGTAGCCAGCGCCGGCCCCCCCCCCCCCCCCGCGGTGCAA‑CGGTGGTATTGCCGGTGATTGCCATCCGGAgg                                        >  1:337538/1‑146 (MQ=255)                                                                                                                         gCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAA‑T‑G‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTAt                              <  1:562767/150‑1 (MQ=255)                                                                                                                         gCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAA‑T‑G‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTAt                              <  1:1018035/150‑1 (MQ=255)                                                                                                                             gagaTAATCGAGATCGGCGGGGGGTC‑GAA‑T‑G‑TCCCCCCTTTTTAATAATATTTGGGCCTCGCGCTGTAGGCAGCGCAGGCCCCCCCCCCCACCCCGCGAGGCAA‑AGGTGGTATAGCCCTTTTACTCCATCAGCAGGaaccaaggatc                             >  2:259207/1‑137 (MQ=255)                                                                                                                              agaTAATCGAGATCGGCGGGGGGTC‑GAA‑TGG‑CCCCCTTTTTCAAAAAATTTTTG‑GCCCCCCGGCGGAGCCAGCCAAGGCCCCCCCCCCCACCCCCCGCTGCAACCGTTGG‑ATTCCCCTTGATCGCCCTCCGGAgggggcagggaacgag                          >  2:957936/1‑135 (MQ=255)                                                                                                                                aTAATCGAGATCGGCGGGGGTTC‑GAA‑T‑G‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAAt                      <  1:985290/151‑1 (MQ=255)                                                                                                                                     cGAGATCGGCGGGGGGTC‑GAAGG‑G‑CCCCCTTTTTG‑AATAATTTTTTGGCCGCGCCCTGTAGCCAGCGCAGGCCCCCCCCCCCACCCCCCGCTGCAA‑CGTTGGTGTCGCCGTTGGTTGCCCTCAGTAGGAGCAAGGTATCGAGCAATGcc                   >  2:1038837/1‑149 (MQ=255)                                                                                                                                       agaTCGGCGGGGGTTC‑GAA‑T‑G‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGtt               <  1:1034376/151‑1 (MQ=255)                                                                                                                                        gaTCGGCGGGGGTTC‑GAA‑T‑G‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTc              <  2:961092/151‑1 (MQ=255)                                                                                                                                                   gTTC‑GAA‑T‑G‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAAcc   >  1:254283/1‑151 (MQ=255)                                                                                                                                                    ttC‑GAA‑T‑G‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAAccc  >  2:1010145/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                            ATATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAA‑T‑G‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCC  >  NC_000913/646600‑646897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑