Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,326,450 | 0 | T | G | 46.4% | 95.9 / 12.8 | 56 | G114G (GGT→GGG) | atoB | acetyl‑CoA acetyltransferase |
Reads supporting (aligned to +/- strand): ref base T (1/29); new base G (26/0); total (27/29) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.06e-15 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTACCGCCGA > NC_000913/2326301‑2326596 | ccGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTa < 1:99657/151‑1 (MQ=255) cgcgTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATgg > 2:616964/1‑150 (MQ=255) cAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAata < 2:992820/151‑1 (MQ=255) cAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAat < 1:97330/150‑1 (MQ=255) aGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAata > 1:1011822/1‑150 (MQ=255) ggCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATAT‑g < 1:477574/151‑1 (MQ=255) ggCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATAT‑g < 2:302064/151‑1 (MQ=255) cACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATAT‑GAg < 2:163077/151‑1 (MQ=255) tAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATAT‑GAGTTTAg < 2:955954/150‑1 (MQ=255) aaaaGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATAT‑GAGTTTAGccc < 1:49609/151‑1 (MQ=255) aaaaGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGAAAATAT‑GAGGTTAGccc > 2:81755/1‑151 (MQ=255) aaaGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATAT‑GAGTTTAGcccc < 1:727606/151‑1 (MQ=255) aGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGAAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGGAAAAATGGAGTTATGcccc > 2:647690/1‑150 (MQ=255) gCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATAT‑GAGTTTAGCCCCCta < 1:851768/151‑1 (MQ=255) tGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGAGGAAAATAT‑GAGTTTATCCCCCtcctt > 2:750417/1‑148 (MQ=255) cAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAGAATAT‑GAGTTTTGGCCCCttcttgct > 1:814215/1‑148 (MQ=255) aGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATAT‑GAGTTTAGCCCCCTACTTACTCGAt < 1:388904/151‑1 (MQ=255) aGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATAT‑TGGTTTTGCCCCCTACCTACTc > 1:476888/1‑148 (MQ=255) aGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATAT‑GAGTTTAGCCCCCTACTTACTCg > 1:397956/1‑149 (MQ=255) gAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATAT‑GAGTTTAGCCCCCTACTTACTCGATg < 1:1063272/151‑1 (MQ=255) ggTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATAT‑GAGTTTAGCCCCCTCCTTACTCGATGCaaa > 1:860559/1‑150 (MQ=255) tgtgCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGGAAATAT‑GAGGTTAACCCCCCACTTACTCGATACaaaa > 2:1013899/1‑149 (MQ=255) tgtgCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATTGAAAATAT‑GAGATTAGCCCCCTACTTCAACGAGGCaaaaac > 2:437083/1‑149 (MQ=255) gtgCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAAAT‑GAGTTTAGCCCCCTACTTACTCGATGCaaaaac > 1:441565/1‑148 (MQ=255) gCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCAc > 2:57585/1‑150 (MQ=255) cGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGc < 2:953865/151‑1 (MQ=255) gATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGctc < 1:726622/151‑1 (MQ=255) cACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGt < 1:590965/151‑1 (MQ=255) tAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATAT‑GAGTTTAGCCCCCCACTTTCTTGATGCAAAAAAACGCCCTg > 2:736354/1‑140 (MQ=255) aaaGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTg < 1:137826/151‑1 (MQ=255) aaaGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATAT‑GAGTTTTGCCCCCCACTTACTCGATGCAAAAACACGCTCTGGGTATCGTCtt > 2:846380/1‑150 (MQ=255) aaGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTgg < 2:1011781/151‑1 (MQ=255) aaGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTgg < 2:762320/151‑1 (MQ=255) aTGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGgaga < 2:639050/150‑1 (MQ=255) tgtgGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAAAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCAAGCTCTGGTTATCGTCTTgg > 1:934998/1‑146 (MQ=255) gtgGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACgg < 1:909359/151‑1 (MQ=255) gTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATAT‑GAGTTTAGCCCCCCACTTACTCGATGCAAAAAAACGCCCTGGTTATCGTCTTGGGGACgg > 2:711115/1‑148 (MQ=255) ttCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATAT‑GAGTTTTGCCCCCTACTTACTCGATGCAAAAACAAGCTCTGGTTATTGTCTTGGGGACgg > 2:367674/1‑147 (MQ=255) cGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATAT‑GAGTTTAGCCCCCCACTTACTCGATGCAAAAACACGCTCTGGTTATCGTCTTGGAGACGGACAgg > 1:59777/1‑150 (MQ=255) gcTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTgcgc < 2:791005/151‑1 (MQ=255) gcTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTgcgc < 2:870717/151‑1 (MQ=255) cTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAAAT‑GAGTTTAGCCCCCTACTTAATCGATGCAAAAACACGCCCTGGTTATCGTCTTGGGGACGGACAGGTTTATGAAGTAATCCTgcg > 1:875608/1‑149 (MQ=255) tGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGcgcg < 1:785430/149‑1 (MQ=255) gccgccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATg > 1:493657/1‑151 (MQ=255) cATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATAT‑GAGGTTAGCCCCCTACTTACTCGATGCAAAAGAACGCTCTGGTTATCGTCTTTGAGACGGACAGGTTTATGACGTAATCCTGCGCGGTGGCCTGAtgtg > 2:533881/1‑150 (MQ=255) tCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGGGACGGCCAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGGGCGcc > 1:296101/1‑151 (MQ=255) aggTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGAAAAAAT‑GAGTTTAACCCCCAACCTTCTCGCTGTCAAAACACGCGCCGGTTATTGTCTTTGGGACGGGACGGGTTATGACGTAATCCTTCGCGATGGCCTGCTGTGCGcccaccac > 2:931398/1‑145 (MQ=255) ggTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCAt < 2:501078/150‑1 (MQ=255) tCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGGTATCGTCTTGGAGACGGACAGGGTTATGACGTAATCCTGCGCGGTGGCCTGGTGTGCGCCACCCATgg > 1:917907/1‑150 (MQ=255) gcAGAGCATTGTGGCGGGGGGGATGGAAAATAT‑TAGTTTTTCCCCCTACTTTCTTGATGGAAAAGAACGCTCTGGGTAATGTCTTGGAGACGGGCAGGGTTTTGACGTAATCCCGCGGGAAGGGCTGATGTGCGGCACCCATgggttt > 2:345142/1‑144 (MQ=255) agagCATTGTGGCGGGGGGTATGGAAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCatat < 2:30833/151‑1 (MQ=255) gagCATTGTGGCGGGGGGTATGGAAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATg < 1:632974/151‑1 (MQ=255) gCATTGTGGCGGGGGGGATGGAAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCCCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATggg > 2:51308/1‑151 (MQ=255) aTTGTGGCGGGGGGTATGGAAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGa < 1:423929/151‑1 (MQ=255) gtgGCGGGGGGTATGGAAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGAtt < 1:942028/150‑1 (MQ=255) ggggTATGGAAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTACCGCCGa < 2:786868/151‑1 (MQ=255) | CCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTACCGCCGA > NC_000913/2326301‑2326596 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |