Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,206,435 | 0 | C | T | 47.3% | 39.6 / 33.1 | 55 | intergenic (‑179/‑28) | ttdR/ttdA | transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit |
Reads supporting (aligned to +/- strand): ref base C (5/24); new base T (26/0); total (31/24) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.81e-11 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGACAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGAAAG‑TAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG > NC_000913/3206289‑3206585 | gtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCTCATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAg < 1:913319/150‑1 (MQ=255) tATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGa < 2:976456/150‑1 (MQ=255) tttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTCAGAc > 1:237541/1‑149 (MQ=255) cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGACAAATa < 1:470513/151‑1 (MQ=255) gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTCAGACaaaaa > 1:921007/1‑148 (MQ=255) cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGACAAATACa < 1:168942/151‑1 (MQ=255) aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGACAAATACaa < 2:444915/151‑1 (MQ=255) cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGACAAATACAAAAAC‑TGGAGTTGc < 2:764460/150‑1 (MQ=255) cTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTCAGACAAAAACAAAAAC‑TGGAGTTGTCa > 1:855/1‑150 (MQ=255) tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTCAGACCAAAACAAAAAA‑TGGAGTTGCCa > 2:752971/1‑149 (MQ=255) ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATGTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGACAAATACAAAAAC‑TGGAGTTGCCatgat < 2:848333/149‑1 (MQ=255) aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGACAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGAAAg < 2:992048/151‑1 (MQ=255) atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTCAGAAAAAAAAAAAAAA‑TGGAGTTGGCATGATGAGCGaaa > 2:518699/1‑149 (MQ=255) attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGACAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGAAAG‑ta < 1:550600/150‑1 (MQ=255) cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGACAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGAAAG‑taata < 2:535976/150‑1 (MQ=255) ttATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAAAAAAAAAAAAAAAAC‑AGGGGTTG‑CATGATTAGCGAAAA‑GAAAAAGCaa > 2:352393/1‑150 (MQ=255) ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑ATTTTTCCCAAAAAAACAAAAAA‑CGGGGTTGTCCTGATGAGGGGAAG‑gaataaaaaaa > 1:750670/1‑141 (MQ=255) gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTCAGACAAAAACAAAAAA‑TGGAGTTGCCCTGATGAGCGAAAG‑GAATAAGCAACAGGCAGt > 1:346526/1‑150 (MQ=255) ttGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑ATTTTTCAGAAAAAAACAAAAAC‑TGGAGGTGGCATTATGGGGGAAAT‑GAAAAAacaaca > 2:989979/1‑141 (MQ=255) ttGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAA‑TTTTTTCAGAAAAATAAAAAAACTTGGGGTGGCCTTG‑TGGGCGAAAA‑TAAAAAGCAAAAAGCAgataa > 1:396872/1‑146 (MQ=255) aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTTCAACAAAAACAAAAAA‑TGGGGGTGCCATGGTGAGGGAAAA‑AAATAAACAAAAAGCAGGGAATaa > 2:652666/1‑150 (MQ=255) aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGACAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGAAAG‑TAATAAGCAACAGGCAGTGAATAAg < 1:87528/151‑1 (MQ=255) aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGACAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGAAAG‑TAATAAGCAACAGGCAGTGAATAAg < 2:473738/151‑1 (MQ=255) cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGACAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGAAAG‑TAATAAGCAACAGGCAGTGAATAAGTTg < 1:768066/151‑1 (MQ=255) gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGACAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGAAAG‑TAATAAGCAACAGGCAGTGAATAAGTTGACa < 1:611596/151‑1 (MQ=255) gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑ATTTTTCAGACAAAAACAAAAAA‑TGGAGGTGCCCTGATGAGCGGAAG‑TAATAAGCAACAGGCAGGGAATAAgat > 1:7557/1‑145 (MQ=255) gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGACAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGAAAG‑TAATAAGCAACAGGCAGTGAATAAGTTGACAgaga < 2:890708/150‑1 (MQ=255) aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑ATTTTTCCGACAAAAATAAAAAA‑TGGAGTTGCCATGATGAGCGAAAA‑GAATAAACAAAAAGCAGGGAAAAAAATGAAAGAGAtt > 1:276448/1‑148 (MQ=255) ttACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGA‑TTTTTCCAAACAAAAACAAAAAA‑TGGGGTTTCCCTGATGAGGGAAAG‑TAAAAAGCAACAGGCAGTGAATAAAATGACAaagaattt > 1:879982/1‑145 (MQ=255) tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTCAAACAAAAAAAAAAAA‑AGGAGTTTCCATGATGAGGGGAAG‑TAAAAAAAAAAAAGCAGAGAATAAGTTTACAGAGAATGt > 2:554676/1‑148 (MQ=255) cATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑ATTTTTCAAACAAAAAAAAAAAA‑TGGAGGTGGCCTGATGAGCGGAAG‑TAATAAACAAAAGGCAGTGAATAAGTTGACAGAGATTGTCGCt > 2:42577/1‑150 (MQ=255) atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTCAAAAAAAAAAAAAAAA‑TGGAGGTTCCCTTATGGGGGGAAA‑GAATAAACAAAAAGCAAGGAAAAAATTTACAGAGATTTTCGCT‑aa > 2:873831/1‑149 (MQ=255) ttCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTCAGACAAAAACAAAAAC‑TGGAGTTGCCATGATGAGGGGAAG‑GAATAAACAACAAGCAGGGAATAAAGTGACAGAGATTGTCGCT‑AACtt > 1:836589/1‑151 (MQ=255) tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGACAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGAAAG‑TAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACtt < 2:1051845/150‑1 (MQ=255) tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑ATTTTTCAGACAAAAAAAAAAAA‑AGGAGTTGGCATGATGAGGGGAAA‑GAATAAGAAACAAGCAGGGGAAAAATTGACAGAGATTGTTGCT‑AACTTTACCGcc > 1:439113/1‑151 (MQ=255) ggCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTCAGACAAAAACAAAAAA‑AGGAGTTGTCCTGATGAGCGAAAA‑TAAAAAACAAAAAGCAGTGGATAAGTTTAAAGAGATTGTTGCT‑AACTTTACcccc > 1:183934/1‑150 (MQ=255) cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑ATTTTTCAGAAAAAAAAAAAAAA‑TTGAGATTGCATGATGGGAGGAAGAAAATAATCACCA‑GCAGGGGAAAAATTGAAAGAGAATTTCGCTAAACTTTAc > 2:546950/1‑145 (MQ=255) aaaaTATAAAACCACATTTTTAGTGGTATTTTGTGGCGGTGAA‑TTTTTCCAGACAAATACAAAAAC‑TGGAGTTGCAATGATGAGCGAAAG‑TAATAACCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑ACCTTTACCGCCATGAtt < 2:715868/151‑1 (MQ=255) aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGA‑TTTTTTCAGACAAAAAAAAAAAA‑TGGAGGTGCCATGATGAGGGAAAG‑GAATAAACAACAAGCAGGGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCCCCCTGAtt > 1:379580/1‑151 (MQ=255) atatAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTGA‑TTTTTTCAAACAAAAACAAAAAA‑TGGAGTTGTCATTATGTGGGGAAG‑TAAAAAACAACAAGCAGTGAATAAATTTTCAAAGAATTTTGCT‑AAATTTTCCCCCCTGtttttt > 2:430557/1‑149 (MQ=255) cacaTTTTTAGTGGTAGTTTGTGGCGGTTTA‑TTTTTTCCGACAAATAAAAAAAA‑TTGAGGTGGCATGATGAGCGAAAG‑AAATAAACAACAAGCAGTGAATAAATTGACAGAGATTTTCGCT‑AAATTTACCCCCATAATTTTTACCCCaa > 1:806689/1‑149 (MQ=255) cacaTTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTCAGACAAAAAAAAAAAA‑AGGAGGTGGCATGATGAGGGGAAG‑TAATAAAAAAAAAGCAGTGAAAAAAATGACAGAGAATGTCGCT‑AAATTTAACGCCATGATTTTTACCCGaa > 2:908900/1‑149 (MQ=255) aTTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTCCGACAAAAACAAAAAC‑TGGAGTTGCCCTGATGAGCGGAAA‑TAAAAAACAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTTCCGCCATGATTTCTACCCGAAAGcc > 1:537569/1‑150 (MQ=255) tttttAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTCAAACAAATACAAAAAA‑TGGAGGTGCCATGATGAGGGAAAG‑TAATAAACAAAAAGCAATGAATAAGTTTACAGAGATTGTCGCT‑AACTTTTACGCCATGATTTTTAACCGAATGCCtg > 1:347990/1‑151 (MQ=255) tttttAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGACAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGAAAG‑TAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCt < 2:320653/150‑1 (MQ=255) ggTAGTTTGTGGCGGTGAA‑TTTTTCCAGACAAATACAAAAAC‑GGGAGTTGCCATGATGAGCGAAAG‑TAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg < 1:683710/151‑1 (MQ=255) ggTAGTTTGTGGCGGTAAA‑TTTTTCCAGACAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGAAAG‑TAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgt < 2:143002/150‑1 (MQ=255) tttGTGGCGGTGAA‑TTTTTCCAGACAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGAAAG‑TAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtggtg < 2:868074/149‑1 (MQ=255) gtgGCGGTGAA‑TTTTTCCAGACAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGAAAG‑TAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGAt < 1:124090/149‑1 (MQ=255) ggCGGTGAA‑TTTTTCCAGACAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGAAAG‑TAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAAc > 1:798950/1‑151 (MQ=255) ggCGGTGAA‑TTTTTCCAGACAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGAAAG‑TAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAAc < 1:790903/151‑1 (MQ=255) cGGTGAA‑TTTTTCCAGACAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGAAAG‑TAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACt > 1:804723/1‑150 (MQ=255) ggTAAA‑TTTTTCCAGAAAAAAAAAAAACC‑AGGATTGGCAATGAGGAGCAAAAG‑AAATAACCAACAGGCAGTGAATAAATTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTTGATa < 2:670667/145‑1 (MQ=255) tttCCAGACAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGAAAG‑TAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt > 1:1020783/1‑150 (MQ=255) tCCAGACAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGAAAG‑TAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa > 2:384251/1‑150 (MQ=255) ccAGACAAATACAAAAAC‑GGGAGTTGCCATGATGAGCGAAAG‑TAAAAAGCAACAGGCAGTGAATAAGTTGACAGAGTTTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAg < 2:743298/151‑1 (MQ=255) ccAGACAAATAAAAAAAC‑TGGAGTTGCCATGATGAGCGAAAG‑TAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa < 2:514522/149‑1 (MQ=255) | GTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGACAAATACAAAAAC‑TGGAGTTGCCATGATGAGCGAAAG‑TAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG > NC_000913/3206289‑3206585 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |