Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4350CT47.3% 39.6 / 33.1 55intergenic (‑179/‑28)ttdR/ttdAtranscriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (5/24);  new base T (26/0);  total (31/24)
Fisher's exact test for biased strand distribution p-value = 7.81e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG  >  NC_000913/3206289‑3206585
                                                                                                                                                   |                                                                                                                                                         
gtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCTCATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAg                                                                                                                                                        <  1:913319/150‑1 (MQ=255)
 tATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGa                                                                                                                                                       <  2:976456/150‑1 (MQ=255)
   tttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAc                                                                                                                                                      >  1:237541/1‑149 (MQ=255)
      cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATa                                                                                                                                                 <  1:470513/151‑1 (MQ=255)
       gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACaaaaa                                                                                                                                                 >  1:921007/1‑148 (MQ=255)
        cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACa                                                                                                                                               <  1:168942/151‑1 (MQ=255)
         aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaa                                                                                                                                              <  2:444915/151‑1 (MQ=255)
                       cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGc                                                                                                                                <  2:764460/150‑1 (MQ=255)
                         cTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAACTGGAGTTGTCa                                                                                                                              >  1:855/1‑150 (MQ=255)
                          tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACCAAAACAAAAAATGGAGTTGCCa                                                                                                                              >  2:752971/1‑149 (MQ=255)
                              ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATGTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatgat                                                                                                                          <  2:848333/149‑1 (MQ=255)
                                     aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAg                                                                                                                 <  2:992048/151‑1 (MQ=255)
                                      atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAAAAAAAAATGGAGTTGGCATGATGAGCGaaa                                                                                                                  >  2:518699/1‑149 (MQ=255)
                                        attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGta                                                                                                              <  1:550600/150‑1 (MQ=255)
                                           cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaata                                                                                                           <  2:535976/150‑1 (MQ=255)
                                                ttATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAAAAAAAAAAAAAAAACAGGGGTTGCATGATTAGCGAAAAGAAAAAGCaa                                                                                                      >  2:352393/1‑150 (MQ=255)
                                                     ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCCAAAAAAACAAAAAACGGGGTTGTCCTGATGAGGGGAAGgaataaaaaaa                                                                                                     >  1:750670/1‑141 (MQ=255)
                                                        gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGTTGCCCTGATGAGCGAAAGGAATAAGCAACAGGCAGt                                                                                              >  1:346526/1‑150 (MQ=255)
                                                           ttGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGAAAAAAACAAAAACTGGAGGTGGCATTATGGGGGAAATGAAAAAacaaca                                                                                                    >  2:989979/1‑141 (MQ=255)
                                                           ttGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAATTTTTTCAGAAAAATAAAAAAACTTGGGGTGGCCTTGTGGGCGAAAATAAAAAGCAAAAAGCAgataa                                                                                           >  1:396872/1‑146 (MQ=255)
                                                              aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTCAACAAAAACAAAAAATGGGGGTGCCATGGTGAGGGAAAAAAATAAACAAAAAGCAGGGAATaa                                                                                        >  2:652666/1‑150 (MQ=255)
                                                              aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAg                                                                                       <  1:87528/151‑1 (MQ=255)
                                                              aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAg                                                                                       <  2:473738/151‑1 (MQ=255)
                                                                 cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTg                                                                                    <  1:768066/151‑1 (MQ=255)
                                                                    gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACa                                                                                 <  1:611596/151‑1 (MQ=255)
                                                                    gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAACAAAAAATGGAGGTGCCCTGATGAGCGGAAGTAATAAGCAACAGGCAGGGAATAAgat                                                                                     >  1:7557/1‑145 (MQ=255)
                                                                         gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                                             <  2:890708/150‑1 (MQ=255)
                                                                             aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCGACAAAAATAAAAAATGGAGTTGCCATGATGAGCGAAAAGAATAAACAAAAAGCAGGGAAAAAAATGAAAGAGAtt                                                                           >  1:276448/1‑148 (MQ=255)
                                                                              ttACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTCCAAACAAAAACAAAAAATGGGGTTTCCCTGATGAGGGAAAGTAAAAAGCAACAGGCAGTGAATAAAATGACAaagaattt                                                                         >  1:879982/1‑145 (MQ=255)
                                                                               tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAAAAAAAAAGGAGTTTCCATGATGAGGGGAAGTAAAAAAAAAAAAGCAGAGAATAAGTTTACAGAGAATGt                                                                         >  2:554676/1‑148 (MQ=255)
                                                                                 cATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAAAAAAAAATGGAGGTGGCCTGATGAGCGGAAGTAATAAACAAAAGGCAGTGAATAAGTTGACAGAGATTGTCGCt                                                                     >  2:42577/1‑150 (MQ=255)
                                                                                    atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAAAAAAAAAAAAAAATGGAGGTTCCCTTATGGGGGGAAAGAATAAACAAAAAGCAAGGAAAAAATTTACAGAGATTTTCGCTaa                                                                  >  2:873831/1‑149 (MQ=255)
                                                                                     ttCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAACTGGAGTTGCCATGATGAGGGGAAGGAATAAACAACAAGCAGGGAATAAAGTGACAGAGATTGTCGCTAACtt                                                               >  1:836589/1‑151 (MQ=255)
                                                                                      tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACtt                                                               <  2:1051845/150‑1 (MQ=255)
                                                                                            tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAAAAAAAAAGGAGTTGGCATGATGAGGGGAAAGAATAAGAAACAAGCAGGGGAAAAATTGACAGAGATTGTTGCTAACTTTACCGcc                                                        >  1:439113/1‑151 (MQ=255)
                                                                                             ggCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAAAGGAGTTGTCCTGATGAGCGAAAATAAAAAACAAAAAGCAGTGGATAAGTTTAAAGAGATTGTTGCTAACTTTACcccc                                                        >  1:183934/1‑150 (MQ=255)
                                                                                               cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGAAAAAAAAAAAAAATTGAGATTGCATGATGGGAGGAAGAAAATAATCACCAGCAGGGGAAAAATTGAAAGAGAATTTCGCTAAACTTTAc                                                            >  2:546950/1‑145 (MQ=255)
                                                                                                  aaaaTATAAAACCACATTTTTAGTGGTATTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCAATGATGAGCGAAAGTAATAACCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTACCTTTACCGCCATGAtt                                                  <  2:715868/151‑1 (MQ=255)
                                                                                                  aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAAAAAAAAAAATGGAGGTGCCATGATGAGGGAAAGGAATAAACAACAAGCAGGGAATAAGTTGACAGAGATTGTCGCTAACTTTACCCCCCTGAtt                                                  >  1:379580/1‑151 (MQ=255)
                                                                                                     atatAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTGATTTTTTCAAACAAAAACAAAAAATGGAGTTGTCATTATGTGGGGAAGTAAAAAACAACAAGCAGTGAATAAATTTTCAAAGAATTTTGCTAAATTTTCCCCCCTGtttttt                                               >  2:430557/1‑149 (MQ=255)
                                                                                                              cacaTTTTTAGTGGTAGTTTGTGGCGGTTTATTTTTTCCGACAAATAAAAAAAATTGAGGTGGCATGATGAGCGAAAGAAATAAACAACAAGCAGTGAATAAATTGACAGAGATTTTCGCTAAATTTACCCCCATAATTTTTACCCCaa                                        >  1:806689/1‑149 (MQ=255)
                                                                                                              cacaTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAAAGGAGGTGGCATGATGAGGGGAAGTAATAAAAAAAAAGCAGTGAAAAAAATGACAGAGAATGTCGCTAAATTTAACGCCATGATTTTTACCCGaa                                        >  2:908900/1‑149 (MQ=255)
                                                                                                                 aTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAACAAAAACTGGAGTTGCCCTGATGAGCGGAAATAAAAAACAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTTCCGCCATGATTTCTACCCGAAAGcc                                    >  1:537569/1‑150 (MQ=255)
                                                                                                                  tttttAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAATACAAAAAATGGAGGTGCCATGATGAGGGAAAGTAATAAACAAAAAGCAATGAATAAGTTTACAGAGATTGTCGCTAACTTTTACGCCATGATTTTTAACCGAATGCCtg                                  >  1:347990/1‑151 (MQ=255)
                                                                                                                  tttttAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCt                                   <  2:320653/150‑1 (MQ=255)
                                                                                                                          ggTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg                          <  1:683710/151‑1 (MQ=255)
                                                                                                                          ggTAGTTTGTGGCGGTAAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgt                           <  2:143002/150‑1 (MQ=255)
                                                                                                                               tttGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtggtg                       <  2:868074/149‑1 (MQ=255)
                                                                                                                                  gtgGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGAt                    <  1:124090/149‑1 (MQ=255)
                                                                                                                                    ggCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAAc                >  1:798950/1‑151 (MQ=255)
                                                                                                                                    ggCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAAc                <  1:790903/151‑1 (MQ=255)
                                                                                                                                      cGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACt               >  1:804723/1‑150 (MQ=255)
                                                                                                                                       ggTAAATTTTTCCAGAAAAAAAAAAAACCAGGATTGGCAATGAGGAGCAAAAGAAATAACCAACAGGCAGTGAATAAATTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTTGATa                   <  2:670667/145‑1 (MQ=255)
                                                                                                                                                tttCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt      >  1:1020783/1‑150 (MQ=255)
                                                                                                                                                  tCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa    >  2:384251/1‑150 (MQ=255)
                                                                                                                                                   ccAGACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAAAAAGCAACAGGCAGTGAATAAGTTGACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAg  <  2:743298/151‑1 (MQ=255)
                                                                                                                                                   ccAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa    <  2:514522/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                         
GTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG  >  NC_000913/3206289‑3206585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: