Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,715,479	G→A	T19T (ACG→ACA)	eamB →	cysteine and O‑acetylserine exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,715,479	0	G	A	100.0%	221.6 / NA	58	T19T (ACG→ACA)	eamB	cysteine and O‑acetylserine exporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/23);  total (35/23)

ATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGCATTTCATTTTCACTGGCAGTGATT  >  NC_000913/2715333‑2715623                                                                                                                                                   |                                                                                                                                                 aTCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACAcc                                                                                                                                                >  1:262907/1‑149 (MQ=255) aTCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAg                                                                                                                                              <  2:378010/151‑1 (MQ=255)    gAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGAc                                                                                                                                           <  2:577931/151‑1 (MQ=255)     aaaaTATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGAcc                                                                                                                                          <  1:497282/151‑1 (MQ=255)       aaTATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCg                                                                                                                                         >  1:949610/1‑150 (MQ=255)         tatCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAAc                                                                                                                                      >  2:592/1‑151 (MQ=255)                  tAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATtctc                                                                                                                             >  1:223538/1‑151 (MQ=255)                   aGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATtctc                                                                                                                             <  2:444354/150‑1 (MQ=255)                   aGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATtc                                                                                                                               <  2:5440/148‑1 (MQ=255)                     aaTATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGcc                                                                                                                          >  1:1022595/1‑151 (MQ=255)                     aaTATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGcc                                                                                                                          >  1:104150/1‑151 (MQ=255)                              aCATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGctc                                                                                                                  >  2:469257/1‑150 (MQ=255)                               cattcattTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTg                                                                                                                <  2:218891/151‑1 (MQ=255)                               cattcattTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTg                                                                                                                >  2:676050/1‑151 (MQ=255)                                attcattTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGc                                                                                                               >  1:425505/1‑151 (MQ=255)                                 ttcattTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCt                                                                                                              >  2:526803/1‑151 (MQ=255)                                  tcattTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCt                                                                                                              >  2:441843/1‑150 (MQ=255)                                   cattTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTAc                                                                                                            <  1:776410/151‑1 (MQ=255)                                        ttttAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGc                                                                                                       >  2:1003825/1‑151 (MQ=255)                                        ttttAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGc                                                                                                       >  1:445750/1‑151 (MQ=255)                                          ttAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCa                                                                                                      <  2:371255/150‑1 (MQ=255)                                           tAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCAt                                                                                                     <  1:481836/150‑1 (MQ=255)                                            aaaTGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATgg                                                                                                   <  2:190553/151‑1 (MQ=255)                                              aTGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGa                                                                                                  >  1:1020494/1‑150 (MQ=255)                                                   ccGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGt                                                                                            >  1:580177/1‑151 (MQ=255)                                                   ccGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGt                                                                                            >  1:145449/1‑151 (MQ=255)                                                   ccGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAATGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGt                                                                                            <  2:668764/151‑1 (MQ=255)                                                       gtCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCaa                                                                                         >  2:48258/1‑150 (MQ=255)                                                             tACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTAccc                                                                                  >  1:69602/1‑151 (MQ=255)                                                                  tCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTg                                                                             >  1:454684/1‑151 (MQ=255)                                                                         aaaCAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAg                                                                      <  1:30437/151‑1 (MQ=255)                                                                             agagGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGAt                                                                  >  2:982982/1‑151 (MQ=255)                                                                              gaggagATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATg                                                                 <  2:875525/151‑1 (MQ=255)                                                                              gaggagATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATg                                                                 >  2:795776/1‑151 (MQ=255)                                                                                  agaTAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGCCCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGa                                                                >  2:202607/1‑148 (MQ=255)                                                                                   gaTAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCt                                                            <  1:243110/151‑1 (MQ=255)                                                                                     tAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTgg                                                          >  2:909169/1‑151 (MQ=255)                                                                                        cAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGAt                                                       <  2:466011/151‑1 (MQ=255)                                                                                          aGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGAttt                                                     >  1:344666/1‑151 (MQ=255)                                                                                          aGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGAttt                                                     >  1:775741/1‑151 (MQ=255)                                                                                           gTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCCATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGAtttt                                                    <  2:160808/151‑1 (MQ=255)                                                                                             gACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGAttttt                                                   <  1:203522/150‑1 (MQ=255)                                                                                             gACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGAttttt                                                   <  1:312368/150‑1 (MQ=255)                                                                                                 ccGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGAtt                                               >  2:794496/1‑150 (MQ=255)                                                                                                      ccTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATg                                         <  1:467371/151‑1 (MQ=255)                                                                                                          ttAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTa                                      >  1:263203/1‑150 (MQ=255)                                                                                                                  tttttGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACtgtgtg                               <  1:312944/149‑1 (MQ=255)                                                                                                                   ttttGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCgg                            >  1:267146/1‑151 (MQ=255)                                                                                                                    tttGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCggg                           >  2:1053832/1‑151 (MQ=255)                                                                                                                     ttGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGc                          >  2:183568/1‑151 (MQ=255)                                                                                                                       ggACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGcat                        >  1:485623/1‑151 (MQ=255)                                                                                                                         aCTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGcattt                      >  1:317865/1‑151 (MQ=255)                                                                                                                            tACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGcatttca                    <  1:821123/150‑1 (MQ=255)                                                                                                                                 ccTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGCATTTCATTTTc               >  1:412953/1‑150 (MQ=255)                                                                                                                                 ccTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGCATTTCATTTTc               >  1:842628/1‑150 (MQ=255)                                                                                                                                    gATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGCATTTCATTTTCACt            <  1:876956/150‑1 (MQ=255)                                                                                                                                          cGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGCATTTCATTTTCACTGGCAGTg     <  2:225041/151‑1 (MQ=255)                                                                                                                                             tATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGCATTTCATTTTCACTGGCAGTGAtt  >  2:700742/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                 ATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGCATTTCATTTTCACTGGCAGTGATT  >  NC_000913/2715333‑2715623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑