Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,912,885	A→G	I256T (ATC→ACC)	relA ←	(p)ppGpp synthetase I/GTP pyrophosphokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,912,885	0	A	G	100.0%	212.5 / NA	57	I256T (ATC→ACC)	relA	(p)ppGpp synthetase I/GTP pyrophosphokinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/35);  total (22/35)

GGCAGGTGGCGATAGTGAGTGTGCACTATCCCCAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGG  >  NC_000913/2912741‑2913030                                                                                                                                                 |                                                                                                                                                  ggCAGGTGGCGATAGTGAGTGTGCACTATCCCCAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGtt                                                                                                                                             <  1:870951/151‑1 (MQ=255)  gCAGGTGGCGATAGTGAGTGTGCACTATCCCCAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGttt                                                                                                                                            <  1:631317/151‑1 (MQ=255)  gCAGGTGGCGATAGTGAGTGTGCACTATCCCCAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGttt                                                                                                                                            >  1:477658/1‑151 (MQ=255)  gCAGGTGGCGATAGTGAGTGTGCACTATCCCCAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGttt                                                                                                                                            <  1:438102/151‑1 (MQ=255)   cAGGTGGCGATAGTGAGTGTGCACTATCCCCAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTc                                                                                                                                           <  2:569437/151‑1 (MQ=255)    aGGTGGCGATAGTGAGTGTGCACTATCCCCAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCg                                                                                                                                          >  1:111510/1‑151 (MQ=255)     ggTGGCGATAGTGAGTGTGCACTATCCCCAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCgg                                                                                                                                         <  2:987340/151‑1 (MQ=255)     ggTGGCGATAGTGAGTGTGCACTATCCCCAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCgg                                                                                                                                         >  2:460292/1‑151 (MQ=255)     ggTGGCGATAGTGAGTGTGCACTATCCCCAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCgg                                                                                                                                         >  2:267744/1‑151 (MQ=255)        ggCGATAGTGAGTGTGCACTATCCCCAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGgacg                                                                                                                                      <  1:93688/151‑1 (MQ=255)              agagagtgTGCACTTTCCCCAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCAt                                                                                                                                 >  2:652559/4‑150 (MQ=255)                  agtgTGCACTATCCCCAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACt                                                                                                                            <  2:203800/151‑1 (MQ=255)                      tgCACTATCCCCAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCg                                                                                                                        >  1:883824/1‑151 (MQ=255)                       gCACTATCCCCAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCg                                                                                                                        <  1:795985/150‑1 (MQ=255)                       gCACTATCCCCAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGc                                                                                                                       >  1:854186/1‑151 (MQ=255)                           tATCCCCAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCttt                                                                                                                    >  2:192829/1‑150 (MQ=255)                            aTCCCCAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTa                                                                                                                   <  2:5246/150‑1 (MQ=255)                               cccAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGc                                                                                                               <  2:227618/151‑1 (MQ=255)                                     gCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCa                                                                                                          <  1:280659/150‑1 (MQ=255)                                     gCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAg                                                                                                         <  2:376812/151‑1 (MQ=255)                                         cATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCttt                                                                                                     >  2:1047323/1‑151 (MQ=255)                                          aTAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTc                                                                                                    >  1:643532/1‑151 (MQ=255)                                            aGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCa                                                                                                   <  2:111506/150‑1 (MQ=255)                                             gCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCAt                                                                                                  >  1:667259/1‑150 (MQ=255)                                               aaTCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCAtctc                                                                                               <  1:393084/151‑1 (MQ=255)                                                aTCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCa                                                                                              <  2:584926/151‑1 (MQ=255)                                                  ccTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAg                                                                                             <  1:1030933/150‑1 (MQ=255)                                                    ggTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTGTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCGGGCATCTCAgcgc                                                                                          <  1:326357/150‑1 (MQ=255)                                                     gTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAgcgc                                                                                          <  2:203030/150‑1 (MQ=255)                                                       aaaCGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa                                                                                       <  1:352773/151‑1 (MQ=255)                                                         aCGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCGAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAgcgcgc                                                                                        >  1:867933/1‑148 (MQ=255)                                                              cGGCGACAATACGTACCTCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGa                                                                                  <  2:425793/149‑1 (MQ=255)                                                              cGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGAcc                                                                                >  2:512068/1‑151 (MQ=255)                                                               ggCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCa                                                                               <  1:376417/151‑1 (MQ=255)                                                                     aaTACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGatc                                                                         <  2:574713/151‑3 (MQ=255)                                                                     aaTACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAAc                                                                         >  1:468177/1‑151 (MQ=255)                                                                         cGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCtt                                                                     >  1:845058/1‑151 (MQ=255)                                                                          gTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTc                                                                    <  2:1041067/151‑1 (MQ=255)                                                                               gcacgcacATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGt                                                               >  2:1041942/1‑151 (MQ=255)                                                                                    cacaTCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAgtgt                                                          <  1:257506/151‑1 (MQ=255)                                                                                      caTCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTc                                                        <  2:836827/151‑1 (MQ=255)                                                                                            aCAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTc                                                  <  2:658353/151‑1 (MQ=255)                                                                                                  catcaAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGAc                                             <  2:8710/150‑1 (MQ=255)                                                                                                  catcaAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTACGGACGACCATACACTTCAGCGTAAAAGAATGAAAATTGAATAGAAGAGAGAAAATGACAAACGAAATCGGCGATGTAGTGTTCGCGGTAGAGAc                                             <  1:1064571/150‑1 (MQ=255)                                                                                                     caAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGcc                                          <  1:261147/150‑1 (MQ=255)                                                                                                         ggCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTc                                      >  1:439810/1‑150 (MQ=255)                                                                                                          gCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCAt                                    <  1:805045/151‑1 (MQ=255)                                                                                                           cGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATg                                   >  1:1475/1‑151 (MQ=255)                                                                                                            gAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATgc                                  <  1:967200/151‑1 (MQ=255)                                                                                                             aGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTATGTGTGTTTCGGACGACCATACCCTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATgca                                 <  2:150459/151‑1 (MQ=255)                                                                                                                  cTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGt                            >  1:596057/1‑151 (MQ=255)                                                                                                                    ttttCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGt                            <  1:436608/149‑1 (MQ=255)                                                                                                                        cTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGc                       >  1:129163/1‑150 (MQ=255)                                                                                                                            aTTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTc                  <  1:456802/151‑1 (MQ=255)                                                                                                                                       gATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCg       >  2:1018253/1‑151 (MQ=255)                                                                                                                                          gCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGt     <  1:127133/150‑1 (MQ=255)                                                                                                                                             gTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTtgg  >  1:591735/1‑150 (MQ=255)                                                                                                                                                 |                                                                                                                                                  GGCAGGTGGCGATAGTGAGTGTGCACTATCCCCAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGG  >  NC_000913/2912741‑2913030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑