Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,919,512	G→A	A412V (GCC→GTC)	gudX ←	glucarate dehydratase‑related protein, substrate unknown

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,919,512	0	G	A	100.0%	232.9 / NA	61	A412V (GCC→GTC)	gudX	glucarate dehydratase‑related protein, substrate unknown
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/34);  total (27/34)

CGTAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCAAATCCAGTGGGTATCGAT  >  NC_000913/2919368‑2919657                                                                                                                                                 |                                                                                                                                                  cgtAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCAt                                                                                                                                             >  1:706461/1‑151 (MQ=255) cgtAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCAt                                                                                                                                             >  2:211472/1‑151 (MQ=255)          agaACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCCCCGCCAGGCAGACGTTTATAGACCTCATGTGCCtttt                                                                                                                                    <  1:322876/151‑1 (MQ=255)              cTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTAc                                                                                                                                >  2:1046456/1‑151 (MQ=255)                cATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCt                                                                                                                              <  1:993415/151‑1 (MQ=255)                       aTGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTccc                                                                                                                        >  2:209785/1‑150 (MQ=255)                       aTGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTccc                                                                                                                        <  1:267534/150‑1 (MQ=255)                           ccTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCccagtcc                                                                                                                   >  1:749133/1‑151 (MQ=255)                           ccTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCccagtcc                                                                                                                   >  2:755383/1‑151 (MQ=255)                           ccTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCccagtcc                                                                                                                   >  2:867424/1‑151 (MQ=255)                               tttCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCccagtccagt                                                                                                                <  2:548575/150‑1 (MQ=255)                                ttCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGCACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTc                                                                                                              >  1:709442/1‑151 (MQ=255)                                   aGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCAc                                                                                                           <  2:492472/151‑1 (MQ=255)                                   aGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCAc                                                                                                           >  1:1056713/1‑151 (MQ=255)                                     aaTCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGc                                                                                                         >  2:727941/1‑151 (MQ=255)                                     aaTCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGc                                                                                                         >  1:52599/1‑151 (MQ=255)                                      aTCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGcc                                                                                                        <  2:552094/151‑1 (MQ=255)                                      aTCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGcc                                                                                                        >  2:27308/1‑151 (MQ=255)                                      aTCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGcc                                                                                                        >  1:10812/1‑151 (MQ=255)                                        cAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCa                                                                                                      <  2:752664/151‑1 (MQ=255)                                          aTGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGccc                                                                                                       >  2:837279/1‑148 (MQ=255)                                               ggCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCggg                                                                                               >  1:140782/1‑151 (MQ=255)                                                   gAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGgcgc                                                                                            >  1:897949/1‑150 (MQ=255)                                                   gAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCa                                                                                           <  1:507441/151‑1 (MQ=255)                                                     aaaCGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATc                                                                                         <  2:604533/151‑1 (MQ=255)                                                         gggACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGa                                                                                     <  2:43/151‑1 (MQ=255)                                                           gACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAAc                                                                                   <  1:836898/151‑1 (MQ=255)                                                            aCGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACg                                                                                  <  1:58724/151‑1 (MQ=255)                                                                 tACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCaa                                                                              <  2:25376/150‑1 (MQ=255)                                                                  aCGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAAt                                                                             <  2:549775/150‑1 (MQ=255)                                                                       cAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTcc                                                                       >  2:963368/1‑151 (MQ=255)                                                                        aaaGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTcc                                                                       <  2:376680/150‑1 (MQ=255)                                                                        aaaGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCg                                                                      <  2:209030/151‑1 (MQ=255)                                                                           ggTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGttt                                                                   >  1:543662/1‑151 (MQ=255)                                                                           ggTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGttt                                                                   <  2:576051/151‑1 (MQ=255)                                                                             tCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGttttt                                                                 >  1:1044851/1‑151 (MQ=255)                                                                               cAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTaa                                                               <  1:35418/151‑1 (MQ=255)                                                                                   cGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAAtctc                                                           <  1:46073/151‑1 (MQ=255)                                                                                     gggATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCa                                                         <  2:658271/151‑1 (MQ=255)                                                                                          cAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTTCCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCgg                                                     <  2:226181/150‑1 (MQ=255)                                                                                           aGGTACTGCATCGGACCTGCGTCGTTACGCGCCCCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAt                                                   <  2:1040147/151‑1 (MQ=255)                                                                                               aCTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtttt                                                <  2:232921/150‑1 (MQ=255)                                                                                                      cGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAgg                                        <  1:87000/151‑1 (MQ=255)                                                                                                        gACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCg                                      <  2:103145/151‑1 (MQ=255)                                                                                                           cTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGAc                                    >  1:514496/1‑150 (MQ=255)                                                                                                             gCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACa                                   <  1:239872/149‑1 (MQ=255)                                                                                                             gCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACa                                   <  2:814903/149‑1 (MQ=255)                                                                                                             gCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAAt                                 <  2:140777/151‑1 (MQ=255)                                                                                                               gtcgtTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCg                               >  2:542903/1‑151 (MQ=255)                                                                                                                 cgtTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGc                              <  1:502625/150‑1 (MQ=255)                                                                                                                  gtTACGCGCACCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGccc                            <  1:799561/151‑1 (MQ=255)                                                                                                                          ccccGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAAATTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCa                    <  1:1023266/149‑1 (MQ=255)                                                                                                                           aCCGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCa                    >  2:670557/1‑150 (MQ=255)                                                                                                                            ccGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCaaa                  <  2:336162/151‑1 (MQ=255)                                                                                                                             cGCCAGGCAGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCAAAt                 >  1:720295/1‑151 (MQ=255)                                                                                                                                     aGACGTTTATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCAAATCCAGTggg         <  1:342055/151‑1 (MQ=255)                                                                                                                                          tttATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCAAATCCAGTGGGTATCg    >  2:544719/1‑151 (MQ=255)                                                                                                                                           ttATAGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCAAATCCAGTGGGTATCg    <  1:45140/150‑1 (MQ=255)                                                                                                                                            tataGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCAAATCCAGTGGGTATCGAt  >  1:430076/1‑151 (MQ=255)                                                                                                                                            tataGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCAAATCCAGTGGGTATCGAt  >  1:853753/1‑151 (MQ=255)                                                                                                                                             ataGACCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCAAATCCAGTGGGTATCGAt  >  1:913420/1‑150 (MQ=255)                                                                                                                                                 |                                                                                                                                                  CGTAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCAAATCCAGTGGGTATCGAT  >  NC_000913/2919368‑2919657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑