Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,041,565	A→G	I85V (ATT→GTT)	ygfZ →	iron‑sulfur cluster repair protein, plumbagin resistance

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,565	0	A	G	100.0%	255.4 / NA	65	I85V (ATT→GTT)	ygfZ	iron‑sulfur cluster repair protein, plumbagin resistance
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/33);  total (32/33)

CAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCT  >  NC_000913/3041420‑3041707                                                                                                                                                  |                                                                                                                                               cAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGa                                                                                                                                            >  2:237792/1‑150 (MQ=255)     gAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGgc                                                                                                                                       >  2:705469/1‑151 (MQ=255)     gAAAAATATATGCAGGGTCAGGTGACAGCAGATGGCTGCCAGATGGTGGGAGATCAGCACCTGCTCGCCGCCCATTGCGTCGCCAAAGGTATAATGTGGAGCAATTTACGTCTATTGCGCGACGTCGATGGCTTTGCATGGGTTGAACGgc                                                                                                                                       <  2:159216/151‑1 (MQ=255)         aaTATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGTTTTGCATGGGTTGAACGgcgc                                                                                                                                     >  1:284574/1‑149 (MQ=255)         aaTATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGgcgc                                                                                                                                     >  1:863600/1‑149 (MQ=255)          atatatGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAgc                                                                                                                                  >  1:569968/1‑151 (MQ=255)             tatGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAgcgtg                                                                                                                               >  2:771203/1‑151 (MQ=255)                 cAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGcgtgcgtg                                                                                                                           >  2:364814/1‑151 (MQ=255)                 cAGGCTCAGCTGACAGCAGATGTCAGCCAGATGGCAAAAGATCGGCACCGGCTCCCCGCCCATTGCGACGCCGAAGGTAAAATGGGGAGCAATTTACGTCTGGTCCGCGACGGCGATGGCTTTGCATGGGTTGAACCGCGCAtcgtgtg                                                                                                                             <  2:819598/149‑3 (MQ=255)                  aGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGcgtgcgtg                                                                                                                           >  2:460950/1‑150 (MQ=255)                  aGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGcgtgcgtg                                                                                                                           >  1:191232/1‑150 (MQ=255)                       cAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCg                                                                                                                      <  1:62005/150‑1 (MQ=255)                       cAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGc                                                                                                                     <  2:973888/151‑1 (MQ=255)                         ggTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGc                                                                                                                     >  1:377413/1‑149 (MQ=255)                                 cagATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGctgactga                                                                                                           <  2:900522/151‑1 (MQ=255)                                 cagATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGctgact                                                                                                             >  1:816166/1‑149 (MQ=255)                                  agATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGctgactga                                                                                                           >  1:1053349/1‑150 (MQ=255)                                   gATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTCACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGactgaac                                                                                                         >  1:155862/1‑151 (MQ=255)                                      gTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGactgaactg                                                                                                       <  2:312827/150‑1 (MQ=255)                                      gTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGactgaactg                                                                                                       <  2:233882/150‑1 (MQ=255)                                      gTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGactgaactg                                                                                                       <  1:958005/150‑1 (MQ=255)                                              gATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAata                                                                                               <  2:265723/150‑1 (MQ=255)                                               aTGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATg                                                                                             >  2:720840/1‑151 (MQ=255)                                                tGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGc                                                                                            >  2:643441/1‑151 (MQ=255)                                                 ggCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGc                                                                                            <  2:1025798/150‑1 (MQ=255)                                                 ggCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGc                                                                                            <  2:96377/150‑1 (MQ=255)                                                 ggCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGc                                                                                            <  1:442681/150‑1 (MQ=255)                                                   cAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGt                                                                                         >  2:161430/1‑151 (MQ=255)                                                        gaTCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATtct                                                                                    <  1:1058229/151‑1 (MQ=255)                                                           cAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTctct                                                                                  <  2:825506/150‑1 (MQ=255)                                                           cAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTa                                                                                 <  1:387733/151‑1 (MQ=255)                                                            aGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTa                                                                                 >  1:11290/1‑150 (MQ=255)                                                                ccTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTg                                                                            <  2:371657/151‑1 (MQ=255)                                                                ccTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTg                                                                            >  1:931125/1‑151 (MQ=255)                                                                   gCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGa                                                                           <  1:573860/149‑1 (MQ=255)                                                                   gCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGa                                                                           <  1:247261/149‑1 (MQ=255)                                                                      cgccgcCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCa                                                                        <  2:120403/149‑1 (MQ=255)                                                                       gccgccCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATcg                                                                     >  1:823455/1‑151 (MQ=255)                                                                          gccCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCgcgc                                                                  <  2:902851/151‑1 (MQ=255)                                                                           cccATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGcc                                                                 >  1:676749/1‑151 (MQ=255)                                                                             cATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAg                                                               <  1:681160/151‑1 (MQ=255)                                                                               ttGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAgac                                                             >  1:415847/1‑151 (MQ=255)                                                                                 gCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAgacga                                                           <  2:720020/151‑1 (MQ=255)                                                                                  cGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAgacga                                                           >  2:672841/1‑150 (MQ=255)                                                                                   gACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAgacgac                                                          <  1:987534/150‑1 (MQ=255)                                                                                   gACGCCAAAGGTAAAATGTGGAGCAATTTACGGCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAGCTGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTAGTCTCTAAAGTGACCATCGCGCCAgacg                                                            <  2:541729/148‑1 (MQ=255)                                                                                      gCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAg                                                       <  1:472563/150‑1 (MQ=255)                                                                                             gTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGtgct                                               >  2:968164/1‑151 (MQ=255)                                                                                               aaaaTGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGtgctg                                              <  2:132866/150‑1 (MQ=255)                                                                                                  aTGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTg                                          <  1:872052/151‑1 (MQ=255)                                                                                                            aTTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCgg                                <  2:332023/151‑1 (MQ=255)                                                                                                              ttACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGt                               <  2:801447/150‑1 (MQ=255)                                                                                                               tACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGttt                             >  1:419240/1‑151 (MQ=255)                                                                                                                  gTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTc                           >  1:180171/1‑150 (MQ=255)                                                                                                                  gTCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTc                           >  1:753430/1‑150 (MQ=255)                                                                                                                   tCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTc                           >  1:841200/1‑149 (MQ=255)                                                                                                                   tCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTc                           >  1:739975/1‑149 (MQ=255)                                                                                                                   tCTGTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTc                           >  1:767012/1‑149 (MQ=255)                                                                                                                      gTTCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcg                      <  2:105367/151‑1 (MQ=255)                                                                                                                       ttCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcgc                     >  2:859983/1‑151 (MQ=255)                                                                                                                              aCGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGcgcgccgc              <  2:34951/151‑1 (MQ=255)                                                                                                                               cGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGcgcgccgcg             >  2:1013338/1‑151 (MQ=255)                                                                                                                               cGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGcgcgccgcg             >  1:69772/1‑151 (MQ=255)                                                                                                                                   gATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTg          <  2:765604/150‑1 (MQ=255)                                                                                                                                           tGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtct  <  2:484476/150‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                               CAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCT  >  NC_000913/3041420‑3041707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑