Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,061,828	G→A	E327K (GAA→AAA)	scpA →	methylmalonyl‑CoA mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,061,828	0	G	A	98.6%	272.2 / ‑2.9	71	coding (979/2145 nt)	scpA	methylmalonyl‑CoA mutase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (29/41);  minor base . (1/0);  total (30/41)
Fisher's exact test for biased strand distribution p-value = 4.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

GGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACAC  >  NC_000913/3061681‑3061973                                                                                                                                                    |                                                                                                                                                  ggcatcggcatGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAAc                                                                                                                                                <  2:299109/151‑1 (MQ=255) ggcatcggcatGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAAc                                                                                                                                                >  2:1032538/1‑151 (MQ=255) ggcatcggcatGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAAc                                                                                                                                                >  1:742234/1‑151 (MQ=255)   catcggcatGGAACTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAAc                                                                                                                                                >  1:793658/1‑149 (MQ=255)    atcggcatGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAgg                                                                                                                                             <  2:212552/151‑1 (MQ=255)      cggcatGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGAt                                                                                                                                           <  1:896790/151‑1 (MQ=255)      cggcatGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGAt                                                                                                                                           <  1:665017/151‑1 (MQ=255)         catGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCg                                                                                                                                        <  1:471716/151‑1 (MQ=255)            ggATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGta                                                                                                                                      <  2:211366/150‑1 (MQ=255)              aTCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGtata                                                                                                                                    >  1:798665/1‑150 (MQ=255)                 tGTTTATGAACGTAGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATaaca                                                                                                                                 >  1:97102/1‑150 (MQ=255)                  gTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATaaca                                                                                                                                 <  1:722475/149‑1 (MQ=255)                     tATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGt                                                                                                                             <  2:933879/150‑1 (MQ=255)                        gAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATc                                                                                                                         <  1:1059646/151‑1 (MQ=255)                        gAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATc                                                                                                                         >  2:154067/1‑151 (MQ=255)                         aaCGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATcc                                                                                                                        >  1:770526/1‑151 (MQ=255)                         aaCGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGCCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGCATAACAACGTTATcc                                                                                                                        >  1:796557/1‑151 (MQ=255)                               gCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGcacc                                                                                                                   >  1:522549/1‑150 (MQ=255)                                ccATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGcaccac                                                                                                                 >  1:448294/1‑151 (MQ=255)                                ccATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGcaccac                                                                                                                 <  1:535524/151‑1 (MQ=255)                                    gTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCAt                                                                                                              <  2:671866/150‑1 (MQ=255)                                       gcgtgcgGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGa                                                                                                           <  2:780070/150‑1 (MQ=255)                                        cgtgcgGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGa                                                                                                           >  2:184580/1‑149 (MQ=255)                                        cgtgcgGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAg                                                                                                         <  2:669728/151‑1 (MQ=255)                                        cgtgcgGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAg                                                                                                         <  2:671496/151‑1 (MQ=255)                                          tgcgGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAgcg                                                                                                       >  1:963835/1‑151 (MQ=255)                                           gcgGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAgcgc                                                                                                      <  1:454007/151‑1 (MQ=255)                                              gCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCgctgg                                                                                                   <  1:995615/151‑1 (MQ=255)                                                  gTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGc                                                                                               <  2:579459/151‑1 (MQ=255)                                                   ttatttatGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCg                                                                                              >  1:305877/1‑151 (MQ=255)                                                   ttatttatGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCCGTGTACACACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGGTATCCGCACCACCACTGAAGCgctggct                                                                                                 <  2:327106/148‑1 (MQ=255)                                                    tatttatGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGca                                                                                              <  2:727302/150‑2 (MQ=255)                                                              gCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCgg                                                                                   <  1:143235/151‑1 (MQ=255)                                                                      gTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGt                                                                           <  2:578770/151‑1 (MQ=255)                                                                      gTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGt                                                                           >  1:223676/1‑151 (MQ=255)                                                                          gTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACt                                                                       <  1:211081/151‑1 (MQ=255)                                                                          gTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACt                                                                       >  2:1014295/1‑151 (MQ=255)                                                                            ggATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTg                                                                      <  2:172484/150‑1 (MQ=255)                                                                            ggATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGc                                                                     <  1:323142/151‑1 (MQ=255)                                                                            ggATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGc                                                                     >  1:562299/1‑151 (MQ=255)                                                                             gATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGc                                                                     >  2:264962/1‑150 (MQ=255)                                                                              aTTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCAt                                                                   >  2:770704/1‑151 (MQ=255)                                                                                 tGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATAcc                                                                >  1:391301/1‑151 (MQ=255)                                                                                      cacaGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGc                                                           >  2:917704/1‑151 (MQ=255)                                                                                        caGGACCCGGAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCt                                                         <  2:25262/151‑1 (MQ=255)                                                                                        caGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCt                                                         >  2:164709/1‑151 (MQ=255)                                                                                        caGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCt                                                         >  2:58428/1‑151 (MQ=255)                                                                                           gACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTg                                                      <  2:1024162/151‑1 (MQ=255)                                                                                              ccGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACg                                                   <  1:407675/151‑1 (MQ=255)                                                                                                     cACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAgcgc                                             <  2:503075/150‑1 (MQ=255)                                                                                                     cACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCt                                            <  1:184587/151‑1 (MQ=255)                                                                                                     cACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCt                                            >  1:648832/1‑151 (MQ=255)                                                                                                      aCTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCtt                                           <  2:209602/151‑1 (MQ=255)                                                                                                      aCTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCtt                                           <  2:739401/151‑1 (MQ=255)                                                                                                         ggCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTgg                                         <  1:303035/150‑1 (MQ=255)                                                                                                               gCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGc                                    <  2:611576/149‑1 (MQ=255)                                                                                                                cGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCt                                  >  1:893198/1‑150 (MQ=255)                                                                                                                cGTACCCACTGCCACACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCt                                  >  2:458532/1‑150 (MQ=255)                                                                                                                  tACCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTAcc                               >  2:372946/1‑151 (MQ=255)                                                                                                                   aCCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTAcc                               <  1:1032580/150‑1 (MQ=255)                                                                                                                   aCCCACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTAcc                               <  2:1010240/150‑1 (MQ=255)                                                                                                                    cccACTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCACACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCg                              >  1:382986/1‑150 (MQ=255)                                                                                                                       aCTGCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGAttt                          <  2:174267/151‑1 (MQ=255)                                                                                                                         tGCCAGACCTCAGGCTGGAGCCTGACT‑AACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTtctc                       >  2:615680/1‑151 (MQ=255)                                                                                                                          gCCAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTtct                        <  2:133829/150‑1 (MQ=255)                                                                                                                            cAGACCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAg                     <  1:689620/151‑1 (MQ=255)                                                                                                                               aCCTCAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCAc                  >  1:288867/1‑151 (MQ=255)                                                                                                                                   cAGGCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGc                <  2:666821/149‑1 (MQ=255)                                                                                                                                     ggCTGGAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTg            <  1:106162/151‑1 (MQ=255)                                                                                                                                          gAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGc       >  2:295825/1‑151 (MQ=255)                                                                                                                                          gAGCCTGACTAAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGc       <  1:737380/151‑1 (MQ=255)                                                                                                                                                gactaaACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAacac  <  2:221688/150‑1 (MQ=255)                                                                                                                                                gactaaACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAaca   <  2:1040596/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  GGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACAC  >  NC_000913/3061681‑3061973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑