Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 231,852 T→C V244A (GTA→GCA)  yafD → endo/exonuclease/phosphatase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913231,8520TC100.0% 336.0 / NA 87V244A (GTA→GCA) yafDendo/exonuclease/phosphatase family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/45);  total (42/45)

AGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGAGCGCGGTGGTGGCGTATGAC  >  NC_000913/231704‑231997
                                                                                                                                                    |                                                                                                                                                 
aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCAc                                                                                                                                                 <  1:882725/151‑1 (MQ=255)
  ccGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTg                                                                                                                                               <  1:578124/151‑1 (MQ=255)
   cGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTgg                                                                                                                                              <  1:69722/151‑1 (MQ=255)
   cGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTgg                                                                                                                                              <  1:350830/151‑1 (MQ=255)
    gTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGTGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGt                                                                                                                                             >  1:4728/1‑151 (MQ=255)
    gTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGt                                                                                                                                             <  2:285080/151‑1 (MQ=255)
      aGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGtt                                                                                                                                            <  2:872781/150‑1 (MQ=255)
        aaGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTAcg                                                                                                                                         >  2:840691/1‑151 (MQ=255)
        aaGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTAcg                                                                                                                                         <  2:701352/151‑1 (MQ=255)
         aGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTAcgc                                                                                                                                        <  1:372631/151‑1 (MQ=255)
            aTGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTAcgcgcg                                                                                                                                     <  2:509932/151‑1 (MQ=255)
              gAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACgcgcgc                                                                                                                                    <  1:992240/150‑1 (MQ=255)
              gAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCt                                                                                                                                   >  1:147829/1‑151 (MQ=255)
                  gcgTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCg                                                                                                                               <  1:665505/151‑1 (MQ=255)
                  gcgTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCg                                                                                                                               >  1:533285/1‑151 (MQ=255)
                   cgTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCg                                                                                                                               >  1:1007195/1‑150 (MQ=255)
                       atatCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATcac                                                                                                                          >  2:25902/1‑151 (MQ=255)
                       atatCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATca                                                                                                                           <  1:971863/150‑1 (MQ=255)
                       atatCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATca                                                                                                                           <  1:689660/150‑1 (MQ=255)
                         atCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATcaca                                                                                                                         >  1:392878/1‑150 (MQ=255)
                            gCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATcc                                                                                                                     <  1:104685/151‑1 (MQ=255)
                                   gcgGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTc                                                                                                              >  1:575917/1‑151 (MQ=255)
                                    cgGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCTGATCACAATCCGCTACTCg                                                                                                             >  2:454042/1‑151 (MQ=255)
                                     gggAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGt                                                                                                            >  1:609012/1‑151 (MQ=255)
                                      ggAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGtt                                                                                                           <  1:883794/151‑1 (MQ=255)
                                      ggAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGtt                                                                                                           >  2:476020/1‑151 (MQ=255)
                                      ggAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGtt                                                                                                           >  2:574052/1‑151 (MQ=255)
                                         aaTGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGa                                                                                                         >  1:519392/1‑150 (MQ=255)
                                            gTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAAtt                                                                                                      <  2:835423/150‑1 (MQ=255)
                                              cGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTc                                                                                                     >  2:678192/1‑149 (MQ=255)
                                              cGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAg                                                                                                   <  2:350685/151‑1 (MQ=255)
                                                cTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTc                                                                                                 >  1:1004200/1‑151 (MQ=255)
                                                   cgcCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCg                                                                                              >  1:693549/1‑151 (MQ=255)
                                                    gcCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCgg                                                                                             <  1:511220/151‑1 (MQ=255)
                                                      cAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCa                                                                                           <  1:283606/151‑1 (MQ=255)
                                                       aGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCaa                                                                                          <  2:596/151‑1 (MQ=255)
                                                                  aCCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa                                                                                <  2:1004159/150‑1 (MQ=255)
                                                                    cGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaataa                                                                             <  2:1033223/151‑1 (MQ=255)
                                                                    cGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaataa                                                                             <  2:952044/151‑1 (MQ=255)
                                                                      aagatCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAgg                                                                           >  1:245887/3‑151 (MQ=255)
                                                                         atCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTa                                                                         <  2:1026108/150‑1 (MQ=255)
                                                                           cAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATg                                                                       <  1:402171/150‑1 (MQ=255)
                                                                            aGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTc                                                                     >  1:252448/1‑151 (MQ=255)
                                                                             gcgcCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTa                                                                         >  1:992520/1‑146 (MQ=255)
                                                                              cgcCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAg                                                                   <  2:147824/151‑1 (MQ=255)
                                                                               gcCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAgg                                                                  <  2:901505/151‑1 (MQ=255)
                                                                                ccGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGt                                                                 <  1:738127/151‑1 (MQ=255)
                                                                                 cGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTc                                                                >  1:845139/1‑151 (MQ=255)
                                                                                    cGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTg                                                              <  1:706490/150‑1 (MQ=255)
                                                                                    cGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGc                                                             >  1:641421/1‑151 (MQ=255)
                                                                                     gggCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGcc                                                            >  1:717449/1‑151 (MQ=255)
                                                                                            tGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAggg                                                      <  1:776040/150‑1 (MQ=255)
                                                                                             ggTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGc                                                     >  2:698855/1‑150 (MQ=255)
                                                                                              gTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGc                                                     >  2:381133/1‑149 (MQ=255)
                                                                                               tCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGCTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGc                                                     >  1:754852/1‑148 (MQ=255)
                                                                                                 gCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTAAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGAcc                                                >  1:1026947/1‑151 (MQ=255)
                                                                                                  cccGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGAcc                                                >  2:530597/1‑150 (MQ=255)
                                                                                                   ccGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCaa                                              <  1:256200/151‑1 (MQ=255)
                                                                                                       tCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGtt                                          >  1:909272/1‑151 (MQ=255)
                                                                                                        cGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGtt                                          <  2:873218/150‑1 (MQ=255)
                                                                                                         gATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGttt                                         >  1:246759/1‑150 (MQ=255)
                                                                                                         gATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTg                                        >  1:883702/1‑151 (MQ=255)
                                                                                                            tttGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCATCGTTTGgc                                      <  2:338537/150‑1 (MQ=255)
                                                                                                            tttGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGgcg                                     >  1:392805/1‑151 (MQ=255)
                                                                                                             ttGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGgcg                                     <  2:707007/150‑1 (MQ=255)
                                                                                                              tGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGgcgc                                    <  1:591413/150‑1 (MQ=255)
                                                                                                              tGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCt                                   >  1:10741/1‑151 (MQ=255)
                                                                                                                   tCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTgcg                                <  1:792868/149‑1 (MQ=255)
                                                                                                                    cTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCa                              >  2:24431/1‑150 (MQ=255)
                                                                                                                     tACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCaa                             >  1:124876/1‑150 (MQ=255)
                                                                                                                      aCCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCaaaa                           <  1:625936/151‑1 (MQ=255)
                                                                                                                       ccGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAAc                          <  2:812987/151‑1 (MQ=255)
                                                                                                                        cGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACg                         >  1:434039/1‑151 (MQ=255)
                                                                                                                                 aaCGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGAGCGCgg                <  1:504346/151‑1 (MQ=255)
                                                                                                                                  aCGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGAGCGCggt               <  1:63333/151‑1 (MQ=255)
                                                                                                                                   cGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGAGCGCggtg              <  2:170177/151‑1 (MQ=255)
                                                                                                                                         tGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGAGCGCGGTGGTGGCg        >  1:841831/1‑151 (MQ=255)
                                                                                                                                         tGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGGATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGAGCGCGGtggggg          >  2:764323/1‑149 (MQ=255)
                                                                                                                                           aaGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTGCTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAGACGTGAGCGCGGTGGTGGCGt       <  2:460023/150‑1 (MQ=255)
                                                                                                                                           aaGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGAGCGCGGTGGTGGCGt       >  2:252142/1‑150 (MQ=255)
                                                                                                                                           aaGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGAGCGCGGTGGTGGCGt       >  2:498391/1‑150 (MQ=255)
                                                                                                                                             gCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGAGCGCGGTGGTGGCGTATg    <  2:1000345/151‑1 (MQ=255)
                                                                                                                                              cTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGAGCGCGGTGGTGGCGTATg    >  2:999937/1‑150 (MQ=255)
                                                                                                                                               ttCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGAGCGCGGTGGTGGCGTATGAc  >  2:865590/1‑151 (MQ=255)
                                                                                                                                               ttCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGAGCGCGGTGGTGGCGTATGAc  >  1:199210/1‑151 (MQ=255)
                                                                                                                                                tCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGAGCGCGGTGGTGGCGTATGAc  <  2:1063079/150‑1 (MQ=255)
                                                                                                                                                tCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGAGCGCGGTGGTGGCGTATGAc  <  1:289165/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
AGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGAGCGCGGTGGTGGCGTATGAC  >  NC_000913/231704‑231997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: