Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,280,131	A→G	E406E (GAA→GAG)	kbaZ →	tagatose 6‑phosphate aldolase 1, kbaZ subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,280,131	0	A	G	100.0%	287.4 / NA	76	E406E (GAA→GAG)	kbaZ	tagatose 6‑phosphate aldolase 1, kbaZ subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/43);  total (33/43)

CCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTT  >  NC_000913/3279988‑3280276                                                                                                                                                |                                                                                                                                                  ccGGACAGCCAGATTGATGGGGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGAGCAGCCAGTATCTGCCGCGGCAGTACGTGAAAATTCGCTCCGGCGAGCTGCAGCCAACGCCCCGGGAGCTCATTa                                                                                                                                            <  2:912326/151‑1 (MQ=255)   ggACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATc                                                                                                                                          <  1:483081/151‑1 (MQ=255)   ggACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATc                                                                                                                                          <  2:554495/151‑1 (MQ=255)   ggACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATc                                                                                                                                          <  2:729559/151‑1 (MQ=255)         ccAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCat                                                                                                                                    <  2:928538/151‑1 (MQ=255)           aGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCatat                                                                                                                                  >  2:738440/1‑151 (MQ=255)           aGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCatat                                                                                                                                  >  1:363190/1‑151 (MQ=255)             aTTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTc                                                                                                                                >  2:659413/1‑151 (MQ=255)               tgatgaCGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCa                                                                                                                               <  2:984337/150‑1 (MQ=255)               tgatgaCGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCa                                                                                                                               <  1:872147/150‑1 (MQ=255)                gatgaCGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAgg                                                                                                                             >  1:1047363/1‑151 (MQ=255)                gatgaCGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAg                                                                                                                              <  2:848944/150‑1 (MQ=255)                   gaCGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGAc                                                                                                                           <  2:934814/150‑1 (MQ=255)                        tttCGCTCATCTGATACGTAATATGGCGGATTCACCAATTCCGCTGCCGCTGATGAGCCAGTATCAGCCGCTGCAGAACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTg                                                                                                                     >  2:379541/1‑151 (MQ=255)                          tCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGgc                                                                                                                   >  2:1048581/1‑151 (MQ=255)                          tCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGgc                                                                                                                   >  2:531139/1‑151 (MQ=255)                           cGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGgcg                                                                                                                  >  2:1049417/1‑151 (MQ=255)                              acATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGgcgc                                                                                                                 >  2:963786/2‑149 (MQ=255)                               cATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCa                                                                                                                <  1:895651/149‑1 (MQ=255)                               cATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGt                                                                                                              >  1:902144/1‑151 (MQ=255)                               cATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGt                                                                                                              <  1:939916/151‑1 (MQ=255)                                aTCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGt                                                                                                              >  2:375762/1‑150 (MQ=255)                                    ggTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACcac                                                                                                         <  1:1055270/151‑1 (MQ=255)                                       aCGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACcacaca                                                                                                      <  2:189839/151‑1 (MQ=255)                                                ggCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCtgtg                                                                                               <  1:423321/149‑1 (MQ=255)                                                 gCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGTTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAg                                                                                            <  1:349208/151‑1 (MQ=255)                                                        cACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATa                                                                                     <  2:1011843/151‑1 (MQ=255)                                                         aCCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATaa                                                                                    <  1:830780/151‑1 (MQ=255)                                                         aCCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATa                                                                                     >  2:108706/1‑150 (MQ=255)                                                         aCCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATa                                                                                     >  2:976696/1‑150 (MQ=255)                                                         aCCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATa                                                                                     >  1:1053659/1‑150 (MQ=255)                                                          ccAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATaa                                                                                    <  2:483290/150‑1 (MQ=255)                                                             aTTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCaa                                                                                <  1:414562/151‑1 (MQ=255)                                                               tCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCaaa                                                                               <  2:649093/150‑1 (MQ=255)                                                                       cgctgATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAgg                                                                      >  1:99111/1‑151 (MQ=255)                                                                       cgctgATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAgg                                                                      >  1:319697/1‑151 (MQ=255)                                                                        gctgATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAgg                                                                      <  1:47641/150‑1 (MQ=255)                                                                         ctgATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGa                                                                     >  1:673657/1‑150 (MQ=255)                                                                           gATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAac                                                                   <  2:454102/150‑1 (MQ=255)                                                                           gATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAac                                                                   <  1:375772/150‑1 (MQ=255)                                                                            aTCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAaca                                                                  >  2:441608/1‑150 (MQ=255)                                                                              cAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGc                                                               <  2:777599/151‑1 (MQ=255)                                                                                 ccAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTa                                                             <  1:632791/150‑1 (MQ=255)                                                                                      aTCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCa                                                        <  2:551034/150‑1 (MQ=255)                                                                                        cTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAat                                                     >  1:901074/1‑151 (MQ=255)                                                                                         tGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAat                                                     >  2:217531/1‑150 (MQ=255)                                                                                         tGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAat                                                     >  1:651388/1‑150 (MQ=255)                                                                                           ccGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATAtt                                                  <  2:212826/151‑1 (MQ=255)                                                                                                gCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGtt                                               <  2:932349/149‑1 (MQ=255)                                                                                                  aGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTaa                                           <  2:927746/151‑1 (MQ=255)                                                                                                     aCGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGcc                                        >  1:222563/1‑151 (MQ=255)                                                                                                      cGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCg                                       >  1:478151/1‑151 (MQ=255)                                                                                                       gTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCgg                                      >  1:382258/1‑151 (MQ=255)                                                                                                         gAAAGGTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGa                                     <  1:938054/150‑1 (MQ=255)                                                                                                          aaaGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGAtt                                   >  1:270445/1‑151 (MQ=255)                                                                                                           aaGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATtg                                  <  2:986909/151‑1 (MQ=255)                                                                                                           aaGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATtg                                  <  1:751469/151‑1 (MQ=255)                                                                                                             gTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATtgat                                <  2:682819/151‑1 (MQ=255)                                                                                                             gTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATtgat                                >  2:15945/1‑151 (MQ=255)                                                                                                                cGCTCCGGCGAGCTGCAGCTAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATtgatga                              <  2:405590/150‑1 (MQ=255)                                                                                                                   tCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGc                          >  1:992729/1‑151 (MQ=255)                                                                                                                    ccGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACg                           <  1:348980/149‑1 (MQ=255)                                                                                                                    ccGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGc                          <  2:538751/150‑1 (MQ=255)                                                                                                                     cGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCtg                        <  2:536943/151‑2 (MQ=255)                                                                                                                      ggCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTg                       >  1:354292/1‑151 (MQ=255)                                                                                                                        cGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGAt                     <  2:608177/151‑1 (MQ=255)                                                                                                                        cGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGAt                     <  1:481711/151‑1 (MQ=255)                                                                                                                               cAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGt              <  1:544358/151‑1 (MQ=255)                                                                                                                                  ccAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTc             >  1:141605/1‑149 (MQ=255)                                                                                                                                   cAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAgg          <  2:499340/151‑1 (MQ=255)                                                                                                                                      cGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCg       <  2:888943/151‑1 (MQ=255)                                                                                                                                      cGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCg       >  2:758014/1‑151 (MQ=255)                                                                                                                                        ccACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGc     <  1:685592/151‑1 (MQ=255)                                                                                                                                         cACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCg    >  1:329295/1‑151 (MQ=255)                                                                                                                                          aCGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGt   >  2:589117/1‑151 (MQ=255)                                                                                                                                           tgggAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGtt  >  2:379530/2‑151 (MQ=255)                                                                                                                                                |                                                                                                                                                  CCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTT  >  NC_000913/3279988‑3280276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑