Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,343,858 T→C V160A (GTA→GCA)  lptA → lipopolysaccharide export ABC transporter periplasmic binding protein; Lipid A binding protein; LPS export and assembly protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,343,8580TC98.6% 267.5 / ‑3.7 73V160A (GTA→GCA) lptAlipopolysaccharide export ABC transporter periplasmic binding protein; Lipid A binding protein; LPS export and assembly protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (41/31);  total (42/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

CAGATGCACTACGAACTGGCAAAAGATTTTGTCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGA  >  NC_000913/3343710‑3344002
                                                                                                                                                    |                                                                                                                                                
cAGATGCACTACGAACTGGCAAAAGATTTTGTCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCaa                                                                                                                                                <  2:305525/151‑1 (MQ=255)
  gATGCACTACGAACTGGCAAAAGATTTTGTCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCaaca                                                                                                                                              <  1:491396/151‑1 (MQ=255)
           cGAACTGGCAAAAGATTTTGTCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTg                                                                                                                                     >  2:1005641/1‑151 (MQ=255)
              aCTGGCAAAAGATTTTGTCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTg                                                                                                                                  >  2:351935/1‑151 (MQ=255)
                    aaaaGATTTTGTCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCg                                                                                                                            >  2:872653/1‑151 (MQ=255)
                     aaaGATTTTGTCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGc                                                                                                                           >  1:333262/1‑151 (MQ=255)
                      aaGATTTTGTCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCa                                                                                                                          <  2:407092/151‑1 (MQ=255)
                        gATTTTGTCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGc                                                                                                                        >  2:1242/1‑150 (MQ=255)
                          ttttGTCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGTGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTg                                                                                                                      >  2:236485/1‑151 (MQ=255)
                             tGTCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGc                                                                                                                     >  2:217405/1‑149 (MQ=255)
                              gTCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAgg                                                                                                                  <  2:343195/151‑1 (MQ=255)
                               tCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAgg                                                                                                                  >  2:7182/1‑150 (MQ=255)
                                  ttCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACa                                                                                                               >  2:656528/1‑150 (MQ=255)
                                      gACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACaaaaa                                                                                                           <  1:932352/150‑1 (MQ=255)
                                      gACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAaac                                                                                                          >  1:324162/1‑151 (MQ=255)
                                       aCGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAaac                                                                                                          >  1:183078/1‑150 (MQ=255)
                                         gggTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAaacaac                                                                                                       >  1:571885/1‑151 (MQ=255)
                                         gggTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAaacaac                                                                                                       >  2:614616/1‑151 (MQ=255)
                                          ggTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAaacaac                                                                                                       >  2:365937/1‑150 (MQ=255)
                                          ggTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAacaaca                                                                                                      <  1:771540/151‑1 (MQ=255)
                                            tAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAAcaacaa                                                                                                     >  2:451114/1‑150 (MQ=255)
                                            tAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAAcaacaa                                                                                                     >  1:971754/1‑150 (MQ=255)
                                              aTGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAgg                                                                                                  <  1:573398/151‑1 (MQ=255)
                                              aTGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAgg                                                                                                  <  2:814010/151‑1 (MQ=255)
                                                 cTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACACAGGCAAGCGCGCAACAACCGTTCTGGCGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGcc                                                                                                >  2:215333/1‑150 (MQ=255)
                                                  ttATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGcc                                                                                                >  2:41006/1‑149 (MQ=255)
                                                    aTCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGa                                                                                             <  1:496862/150‑1 (MQ=255)
                                                       tGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGAcccc                                                                                         >  2:860647/1‑151 (MQ=255)
                                                         cagcagGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCgg                                                                                       >  2:339760/1‑151 (MQ=255)
                                                         cagcagGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCgg                                                                                       <  2:720936/151‑1 (MQ=255)
                                                         cagcagGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCgg                                                                                       <  1:732213/151‑1 (MQ=255)
                                                          agcagGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGc                                                                                      <  2:866490/151‑1 (MQ=255)
                                                           gcagGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGca                                                                                     <  1:839422/151‑1 (MQ=255)
                                                             agGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGcaca                                                                                   <  2:198131/151‑1 (MQ=255)
                                                             agGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGcac                                                                                    >  2:331829/1‑150 (MQ=255)
                                                               gTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGcac                                                                                    >  1:96206/1‑148 (MQ=255)
                                                               gTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACaga                                                                                 <  1:214094/151‑1 (MQ=255)
                                                                tCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACag                                                                                  >  2:846493/1‑149 (MQ=255)
                                                                   aTAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACagaag                                                                               >  1:564210/1‑149 (MQ=255)
                                                                     aGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGaagaag                                                                            <  1:159556/150‑1 (MQ=255)
                                                                     aGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAgg                                                                           >  2:650298/1‑151 (MQ=255)
                                                                        aaCATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGt                                                                         >  2:972055/1‑150 (MQ=255)
                                                                         aCATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGta                                                                        >  1:222035/1‑150 (MQ=255)
                                                                          cATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGtaat                                                                      >  2:467366/1‑151 (MQ=255)
                                                                           aTTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGtaatt                                                                     >  2:66421/1‑151 (MQ=255)
                                                                              aaGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGtaattaat                                                                  >  2:122922/1‑151 (MQ=255)
                                                                              aaGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGtaattaat                                                                  <  1:811188/151‑1 (MQ=255)
                                                                                       aaGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATgg                                                         >  2:813402/1‑151 (MQ=255)
                                                                                        aGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGc                                                        <  2:943342/151‑1 (MQ=255)
                                                                                                  ccTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACt                                              >  1:721937/1‑151 (MQ=255)
                                                                                                    tGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGc                                            >  1:232022/1‑151 (MQ=255)
                                                                                                    tGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGc                                            >  1:419721/1‑151 (MQ=255)
                                                                                                     ggTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGc                                            <  2:667291/150‑1 (MQ=255)
                                                                                                     ggTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCa                                           <  2:993487/151‑1 (MQ=255)
                                                                                                        gAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCaaa                                         <  1:493789/150‑1 (MQ=255)
                                                                                                        gAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAg                                        <  2:204900/151‑1 (MQ=255)
                                                                                                         aaaGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCaa                                          >  1:883087/1‑148 (MQ=255)
                                                                                                             agCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCt                                   <  1:168065/151‑1 (MQ=255)
                                                                                                              gCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCtt                                  <  2:691716/151‑1 (MQ=255)
                                                                                                                   aaaTGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCaaa                             <  1:230605/151‑1 (MQ=255)
                                                                                                                    aaTGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCaaaa                            <  1:768207/151‑1 (MQ=255)
                                                                                                                             tttCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATaa                    >  1:965519/1‑150 (MQ=255)
                                                                                                                                cAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACATAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATAAAgg                 <  2:930683/150‑1 (MQ=255)
                                                                                                                                 aGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATAAAGGcc               >  2:580246/1‑151 (MQ=255)
                                                                                                                                 aGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATAAAGGcc               >  2:361628/1‑151 (MQ=255)
                                                                                                                                     aCAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTcg           >  2:522041/1‑151 (MQ=255)
                                                                                                                                       aaaGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTcgcg         <  1:1031226/151‑1 (MQ=255)
                                                                                                                                          ggCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTgg      <  2:947075/151‑1 (MQ=255)
                                                                                                                                           gCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTgg      >  2:582319/1‑150 (MQ=255)
                                                                                                                                            cAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCCGACCCCGGCACAGAAGAATGGTAATTAATTCGTTATGGCAACATTACCTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTa    <  2:805170/151‑1 (MQ=255)
                                                                                                                                             aaGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGt     >  2:582200/1‑149 (MQ=255)
                                                                                                                                             aaGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTag   <  2:960195/151‑1 (MQ=255)
                                                                                                                                                cgcgCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTaga  >  1:948323/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CAGATGCACTACGAACTGGCAAAAGATTTTGTCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGA  >  NC_000913/3343710‑3344002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: