Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,376,927 A→G L165P (CTG→CCG)  sspA ← stringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,376,9270AG98.5% 242.6 / ‑4.1 67L165P (CTG→CCG) sspAstringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (32/34);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

TTAACTCCGGCCCAGACGCATTTCACGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGAT  >  NC_000913/3376782‑3377069
                                                                                                                                                 |                                                                                                                                              
ttAACTCCGGCCCAGACGCATTTCACGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACggcagc                                                                                                                                           >  2:743459/1‑151 (MQ=255)
 tAACTCCGGCCCAGACGCATTTCACGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACggcagc                                                                                                                                           <  2:587153/150‑1 (MQ=255)
         gCCCAGACGCATTTCACGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGa                                                                                                                                  <  2:509585/151‑1 (MQ=255)
           ccAGACGCATTTCACGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGAt                                                                                                                                 >  1:950592/1‑150 (MQ=255)
           ccAGACGCATTTCACGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGAt                                                                                                                                 >  1:301353/1‑150 (MQ=255)
                      tCACGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGAcc                                                                                                                      >  1:367879/1‑150 (MQ=255)
                         cGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGc                                                                                                                  <  2:420691/151‑1 (MQ=255)
                          gTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCt                                                                                                                 >  2:696756/1‑151 (MQ=255)
                          gTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCt                                                                                                                 <  1:588305/151‑1 (MQ=255)
                             cTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGa                                                                                                               >  2:746528/1‑150 (MQ=255)
                             cTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCCTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGa                                                                                                               >  2:874801/1‑150 (MQ=255)
                               gCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAAc                                                                                                             <  2:463327/150‑1 (MQ=255)
                               gCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACt                                                                                                            <  1:325078/151‑1 (MQ=255)
                                   cAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTc                                                                                                           <  2:1029769/148‑1 (MQ=255)
                                   cAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATc                                                                                                        >  1:62691/1‑151 (MQ=255)
                                      ttAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCt                                                                                                     >  1:656021/1‑151 (MQ=255)
                                        aaaGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTc                                                                                                    >  2:691028/1‑150 (MQ=255)
                                             agCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAg                                                                                              <  1:951203/151‑1 (MQ=255)
                                              gCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAg                                                                                              >  2:310776/1‑150 (MQ=255)
                                              gCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATAGACCAGGCTGAACTCATCGCTCAGGAAg                                                                                              >  1:274502/1‑150 (MQ=255)
                                               cAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGt                                                                                             >  1:433236/1‑150 (MQ=255)
                                                aaGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTAc                                                                                           <  2:304491/151‑1 (MQ=255)
                                                    aaaGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCt                                                                                       <  1:654247/151‑1 (MQ=255)
                                                     aaGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCtt                                                                                      >  2:394353/1‑151 (MQ=255)
                                                                tCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGa                                                                              >  2:614667/1‑148 (MQ=255)
                                                                 cAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAg                                                                            <  1:194348/149‑1 (MQ=255)
                                                                 cAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGAc                                                                          <  2:821962/151‑1 (MQ=255)
                                                                    aGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCgg                                                                       >  1:605639/1‑151 (MQ=255)
                                                                       cgcgGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGgcgc                                                                    >  1:279188/1‑151 (MQ=255)
                                                                        gcgGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGgcgc                                                                    >  2:405535/1‑150 (MQ=255)
                                                                        gcgGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGgcgc                                                                    >  2:979701/1‑150 (MQ=255)
                                                                        gcgGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCa                                                                   <  1:868414/151‑1 (MQ=255)
                                                                              cATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGcc                                                             >  1:732515/1‑151 (MQ=255)
                                                                              cATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGcc                                                             <  1:422366/151‑1 (MQ=255)
                                                                                     ccTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGtt                                                      >  2:285517/1‑151 (MQ=255)
                                                                                       tttCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGttct                                                    >  2:73645/1‑151 (MQ=255)
                                                                                          cAGCTCTTTCGCCCCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTcg                                                 <  2:238909/151‑1 (MQ=255)
                                                                                          cAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGTAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTcg                                                 >  1:844253/1‑151 (MQ=255)
                                                                                           aGCTCTTTCGCACCCGGGTCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGCAGACCGGCGCAATCGCCAGCAGTTCTTcgc                                                >  2:436351/1‑151 (MQ=255)
                                                                                            gCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTcgcg                                               <  1:798053/151‑1 (MQ=255)
                                                                                            gCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTcgcg                                               <  2:965768/151‑1 (MQ=255)
                                                                                                tttCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGAACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGt                                           >  2:162327/1‑151 (MQ=255)
                                                                                                 ttCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAg                                            <  2:989788/149‑1 (MQ=255)
                                                                                                  tCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGtt                                          <  1:1012287/150‑1 (MQ=255)
                                                                                                  tCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTg                                         >  1:1048523/1‑151 (MQ=255)
                                                                                                  tCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTg                                         >  2:988035/1‑151 (MQ=255)
                                                                                                    gCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCt                                       >  2:814726/1‑151 (MQ=255)
                                                                                                    gCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCt                                       <  1:631423/151‑1 (MQ=255)
                                                                                                      aCCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCtt                                      >  2:133130/1‑150 (MQ=255)
                                                                                                       cccGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTAc                                    >  2:756223/1‑151 (MQ=255)
                                                                                                         cGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTat                                    <  2:209966/149‑2 (MQ=255)
                                                                                                            gCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCg                               <  2:1031069/151‑1 (MQ=255)
                                                                                                             ccGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCgg                              <  2:35455/151‑1 (MQ=255)
                                                                                                                cTGAACTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCAt                           >  1:319527/1‑151 (MQ=255)
                                                                                                                 tGAACTCGATGCCCAGTGGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGc                             <  2:363002/148‑1 (MQ=255)
                                                                                                                     cTCGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGc                       >  2:998190/1‑150 (MQ=255)
                                                                                                                       cGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCtt                     <  1:195242/150‑1 (MQ=255)
                                                                                                                       cGATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCt                      <  1:352105/149‑1 (MQ=255)
                                                                                                                        gATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCa                   <  2:1052764/151‑1 (MQ=255)
                                                                                                                        gATGCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCa                   <  2:99456/151‑1 (MQ=255)
                                                                                                                           gCCCAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGTCCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGaa                <  1:574285/151‑1 (MQ=255)
                                                                                                                            cccAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAg               <  1:370401/151‑1 (MQ=255)
                                                                                                                              cAGTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCt             >  1:687897/1‑151 (MQ=255)
                                                                                                                                gTTGCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGa           <  2:575342/151‑1 (MQ=255)
                                                                                                                                   gCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAAcc        <  1:438506/151‑1 (MQ=255)
                                                                                                                                   gCGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAAcc        <  2:541815/151‑1 (MQ=255)
                                                                                                                                    cGGCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCg       >  2:945210/1‑151 (MQ=255)
                                                                                                                                      gCAGACGCCACGGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGtt     <  2:35008/151‑1 (MQ=255)
                                                                                                                                         gACGCCACGGCGGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTGGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTtgat  <  1:61781/151‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
TTAACTCCGGCCCAGACGCATTTCACGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGAT  >  NC_000913/3376782‑3377069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: