Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,622,455 T→C S1088S (AGT→AGC rhsB → Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,622,4550TC96.2% 108.5 / NA 52S1088S (AGT→AGCrhsBRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (29/21);  minor base G (0/2);  total (29/23)
Fisher's exact test for biased strand distribution p-value = 1.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

GGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAG  >  NC_000913/3622313‑3622572
                                                                                                                                              |                                                                                                                     
ggAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTGCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGgtgt                                                                                                                <  2:194936/150‑1 (MQ=255)
 gAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTc                                                                                                              >  2:710555/1‑151 (MQ=38)
   gCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTccc                                                                                                            <  1:127746/151‑1 (MQ=37)
           cAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGc                                                                                                    >  2:533209/1‑151 (MQ=25)
                gATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTg                                                                                               >  2:81326/1‑151 (MQ=25)
                 aTTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGc                                                                                              >  1:866404/1‑151 (MQ=25)
                  tttATCAGCGGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGc                                                                                              <  2:458026/150‑1 (MQ=25)
                    tATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAGAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGc                                                                                              >  2:273440/1‑148 (MQ=25)
                       cAGGCGGGGAGCTTCACGCCTCTCATCAGGGTCGAAACTGCCGCCGGTGAGCTGGCGAAAACGCAGCCCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTTTTCCCGCCGGTGCTGGTGCAGATg                                                                                         <  1:370629/150‑1 (MQ=11)
                       cAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGc                                                                                        >  2:544147/1‑151 (MQ=25)
                          ccGGGGAGCTCCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGGGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGCCCGGCGGGGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCt                                                                                       <  2:500567/149‑1 (MQ=255)
                               gAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCgg                                                                                <  1:992278/151‑1 (MQ=25)
                                  cTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTg                                                                             >  2:785383/1‑151 (MQ=25)
                                    tCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTgg                                                                            >  1:64235/1‑150 (MQ=25)
                                    tCACGCCACTCATCAGGGTCGAAACTGCCACCGGCGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTgg                                                                            >  2:84432/1‑150 (MQ=25)
                                     cACGCCACTCAGCAGGGTCGAACCTGCCACCGGTGGGCGGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCGGTCGGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTg                                                                             <  2:214440/148‑1 (MQ=11)
                                       cGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGaaa                                                                         <  1:200396/150‑1 (MQ=25)
                                        gCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGGGAAAACGCAGCGCCGCAGCGTGGCGGATGCTCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGGTCCCGCCGGTGCTGGTGCAGATGCGCGACCGGCTGGAAAg                                                                        >  2:100972/1‑150 (MQ=11)
                                         ccACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGt                                                                       <  2:563647/150‑1 (MQ=25)
                                            cTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGaa                                                                    >  1:996446/1‑150 (MQ=25)
                                                tcaGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAAt                                                                  <  2:1026667/148‑1 (MQ=25)
                                                tcaGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCt                                                               >  1:707905/1‑151 (MQ=25)
                                                  aGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTgg                                                             >  2:9210/1‑151 (MQ=25)
                                                    ggTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCt                                                           >  1:571112/1‑151 (MQ=25)
                                                        gAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGAcc                                                       >  2:970208/1‑151 (MQ=18)
                                                          accggCCACGGGTGAGCGGGCGAAACCGCGGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGGGGCGAAGAGGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCgg                                                     <  2:721667/147‑1 (MQ=11)
                                                              gCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGgtg                                                  >  1:1026735/1‑150 (MQ=14)
                                                               ccACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGGGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGgtg                                                  >  1:335918/1‑149 (MQ=14)
                                                                cACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGgtgag                                                >  2:682950/1‑150 (MQ=14)
                                                                    ggTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAgg                                           >  1:847103/1‑151 (MQ=14)
                                                                         gcgggCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGt                                                                       <  2:950570/115‑1 (MQ=17)
                                                                         gCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGt                                                                       >  1:950610/1‑118 (MQ=17)
                                                                         gCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAg                                       <  1:964093/150‑1 (MQ=14)
                                                                          cTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAgcc                                     >  1:53206/1‑151 (MQ=14)
                                                                            ggCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAgccg                                    <  2:380766/150‑1 (MQ=14)
                                                                            ggCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGCCCGGCGGCGAAGACGGTGGCAGCGTGGTGTCCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAg                                       <  2:682511/147‑1 (MQ=9)
                                                                              cGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGccgccg                                 >  2:291116/1‑151 (MQ=14)
                                                                               gAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCcgccgc                                >  1:889080/1‑151 (MQ=14)
                                                                                 aaccacggcgcccaggCCTGGGGGATGCCCTTCACGAGCCCGGCGCGGAAGGGGGGGGCAGGGTGGTGTCCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCgctg                              <  1:121615/136‑1 (MQ=11)
                                                                                                gCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCgg               >  2:972254/1‑151 (MQ=14)
                                                                                                 ccgggCGGATGCCCTTCAGCAGTCCGGCGGCGAAGCCGGTGGCAGCGGGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCgg               <  2:642718/147‑1 (MQ=9)
                                                                                                   tGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCt            >  1:575701/1‑151 (MQ=14)
                                                                                                    gggggATGCCCTTCAGCATGCCGGCGGCGAGGAGGGTGGCGGGGTGGTGTCCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCt            <  2:236242/147‑1 (MQ=11)
                                                                                                    ggCGGATGCCCTTCAGCAGCCCGGCGGCGAAGACGGGGGCAGCGGGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCt            <  2:282495/150‑1 (MQ=9)
                                                                                                     gCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTg           >  1:1012013/1‑150 (MQ=14)
                                                                                                      cGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGAc         >  2:641312/1‑151 (MQ=14)
                                                                                                       ggATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGAcc        <  1:81327/151‑1 (MQ=14)
                                                                                                         aTGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGt      <  1:310525/151‑1 (MQ=14)
                                                                                                            cccTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTgg    >  1:534183/1‑150 (MQ=14)
                                                                                                            cccTACAGCAGCCCGGGGGAGAAGACGTTGGAAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGa   <  2:722696/151‑1 (MQ=11)
                                                                                                             ccTTCAGCGGTCGGGGGGCGAAGAGGGTGGCGGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAg  <  2:7761/151‑1 (MQ=11)
                                                                                                             ccTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGCGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTgg    >  1:176696/1‑149 (MQ=11)
                                                                                                                                              |                                                                                                                     
GGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAG  >  NC_000913/3622313‑3622572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: