Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,856,193	G→A	L39F (CTT→TTT)	yidH ←	DUF202 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,856,193	0	G	A	100.0%	266.7 / NA	71	L39F (CTT→TTT)	yidH	DUF202 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/34);  total (37/34)

GCAGGTCCTCTTTCAGACGCATCGCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTA  >  NC_000913/3856046‑3856339                                                                                                                                                    |                                                                                                                                                   gCAGGTCCTCTTTCAGACGCATCGCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAAccc                                                                                                                                                 <  1:633092/151‑1 (MQ=255)      tCCTCTTTCAGACGCATCGCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAaacccaaccc                                                                                                                                            >  2:45734/1‑151 (MQ=255)      tCCTCTTTCAGACGCATCGCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAaacccaaccc                                                                                                                                            >  2:830310/1‑151 (MQ=255)       ccTCTTTCAGACGCATCGCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAAcccc                                                                                                                                           >  1:332430/1‑151 (MQ=255)       ccTCTTTCAGACGCATCGCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACACAAcccc                                                                                                                                           >  2:338798/1‑151 (MQ=255)       ccTCTTTCAGACGCACCGCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAAcct                                                                                                                                            <  2:1040123/150‑2 (MQ=255)          ctTTCAGACGCATCGCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCccgc                                                                                                                                        >  2:349378/1‑151 (MQ=255)          ctTTCAGACGCATCGCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCccgc                                                                                                                                        >  2:184051/1‑151 (MQ=255)             tCAGACGCATCGCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCcgccgc                                                                                                                                     >  2:689021/1‑151 (MQ=255)                  cGCATCGCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGaa                                                                                                                                <  2:219721/151‑1 (MQ=255)                  cGCATCGCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGa                                                                                                                                 >  1:518016/1‑150 (MQ=255)                      tCGCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATcc                                                                                                                            >  1:284915/1‑151 (MQ=255)                           ttttCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGt                                                                                                                        <  1:1053683/150‑1 (MQ=255)                              tCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCa                                                                                                                     <  1:348103/150‑1 (MQ=255)                               cATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAg                                                                                                                    <  2:928554/150‑1 (MQ=255)                                  tGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACg                                                                                                                >  2:341231/1‑151 (MQ=255)                                   gCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACgg                                                                                                               <  2:475023/151‑1 (MQ=255)                                    cGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACgg                                                                                                               >  1:678696/1‑150 (MQ=255)                                    cGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGa                                                                                                              <  2:484984/151‑1 (MQ=255)                                     gTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGAt                                                                                                             >  2:910336/1‑151 (MQ=255)                                       aaCCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATcc                                                                                                           >  1:816796/1‑151 (MQ=255)                                          cAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCa                                                                                                          <  2:529471/149‑1 (MQ=255)                                                aaGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGaa                                                                                                  <  1:730389/151‑1 (MQ=255)                                                aaGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGa                                                                                                   >  2:194385/1‑150 (MQ=255)                                                aaGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGa                                                                                                   >  1:341196/1‑150 (MQ=255)                                                 aGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGaaa                                                                                                 <  1:329391/151‑1 (MQ=255)                                                     gCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATATGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGt                                                                                              >  2:79565/1‑150 (MQ=255)                                                      ccATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTAc                                                                                            >  1:334135/1‑151 (MQ=255)                                                         tACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGc                                                                                          >  2:143965/1‑150 (MQ=255)                                                          aCATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGc                                                                                          <  2:568905/149‑1 (MQ=255)                                                          aCATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTc                                                                                        >  1:466960/1‑151 (MQ=255)                                                           cATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCa                                                                                       <  1:194393/151‑1 (MQ=255)                                                                 tAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTa                                                                                    <  1:5545/148‑1 (MQ=255)                                                                   aCCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAg                                                                               <  2:128078/151‑1 (MQ=255)                                                                      cgccgGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCg                                                                             >  2:525521/1‑150 (MQ=255)                                                                         cgGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCgaga                                                                          >  2:206813/1‑150 (MQ=255)                                                                           gAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGc                                                                       >  1:964990/1‑151 (MQ=255)                                                                           gAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGc                                                                       >  2:622146/1‑151 (MQ=255)                                                                            aaaaCAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCg                                                                      >  1:921198/1‑151 (MQ=255)                                                                                cAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTa                                                                   >  1:683851/1‑150 (MQ=255)                                                                                  gACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAAt                                                                 <  2:588815/150‑1 (MQ=255)                                                                                         caacaacGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCACCCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCt                                                         <  1:124416/151‑1 (MQ=255)                                                                                          aacaacGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCtt                                                        <  2:367424/151‑1 (MQ=255)                                                                                            caacGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTtct                                                      <  1:921967/151‑1 (MQ=255)                                                                                            caacGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTtct                                                      >  2:781420/1‑151 (MQ=255)                                                                                              acGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTcc                                                    >  2:25921/1‑151 (MQ=255)                                                                                              acGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTcc                                                    <  2:907223/151‑1 (MQ=255)                                                                                                gCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGa                                                  <  1:427719/151‑1 (MQ=255)                                                                                                gCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGa                                                  <  1:842685/151‑1 (MQ=255)                                                                                                    gcagTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGc                                               <  2:999165/150‑1 (MQ=255)                                                                                                    gcagTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCg                                              <  2:770884/151‑1 (MQ=255)                                                                                                    gcagTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCg                                              <  1:801636/151‑1 (MQ=255)                                                                                                     cagTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCgg                                             <  2:770706/151‑1 (MQ=255)                                                                                                         tCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGaa                                          <  1:373660/150‑1 (MQ=255)                                                                                                         tCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGaa                                          >  2:100008/1‑150 (MQ=255)                                                                                                          cgcgAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGaaa                                         <  2:652673/150‑1 (MQ=255)                                                                                                          cgcgAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAAt                                        <  1:59332/151‑1 (MQ=255)                                                                                                            cgAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAAttt                                      >  1:508673/1‑151 (MQ=255)                                                                                                              aaTGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTc                                    <  2:850867/151‑1 (MQ=255)                                                                                                                  aCGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCAtt                                 >  1:15567/1‑150 (MQ=255)                                                                                                                  aCGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCAtt                                 >  2:170644/1‑150 (MQ=255)                                                                                                                        gTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTc                          >  2:574980/1‑151 (MQ=255)                                                                                                                          ggCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTcc                         <  2:470049/150‑1 (MQ=255)                                                                                                                          ggCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCt                        >  1:95527/1‑151 (MQ=255)                                                                                                                            cGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCttt                      >  1:455644/1‑151 (MQ=255)                                                                                                                             gAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTg                     <  1:258247/151‑1 (MQ=255)                                                                                                                                    ggCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCaa                  <  1:574995/147‑1 (MQ=255)                                                                                                                                         aaGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTTATTGTTGCCCCTTTGCAAttt               >  2:639300/1‑145 (MQ=255)                                                                                                                                            ttGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGc      >  2:727443/1‑151 (MQ=255)                                                                                                                                            ttGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGc      >  1:637228/1‑151 (MQ=255)                                                                                                                                                tCAAACCCAACACCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCCCATTATCCAGCGAGAAGCGGTAATCAGGCGCATCTCCGAGGCGGGAAAATTTCATTGGTGATCCTTTGTAATTTTACTAAAActct   >  1:517952/1‑150 (MQ=255)                                                                                                                                                 cAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGctct   >  1:523234/1‑149 (MQ=255)                                                                                                                                                 cAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTa  <  2:753139/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GCAGGTCCTCTTTCAGACGCATCGCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTA  >  NC_000913/3856046‑3856339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑