Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,899,764	C→T	G272S (GGC→AGC)	yieL ←	putative xylanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,899,764	0	C	T	100.0%	262.8 / NA	65	G272S (GGC→AGC)	yieL	putative xylanase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/51);  total (14/51)

GTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCT  >  NC_000913/3899618‑3899907                                                                                                                                                   |                                                                                                                                                gTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCg                                                                                                                                             >  1:1052943/1‑151 (MQ=255)    cAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGc                                                                                                                                           <  2:236378/150‑1 (MQ=255)     aGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCAt                                                                                                                                         <  1:1019489/151‑1 (MQ=255)     aGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCAt                                                                                                                                         <  2:220529/151‑1 (MQ=255)        cGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCtt                                                                                                                                      <  1:1001171/151‑1 (MQ=255)              gcgACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGa                                                                                                                                <  1:192239/151‑1 (MQ=255)               cgACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGAc                                                                                                                               <  2:647334/151‑1 (MQ=255)                    ccTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTaa                                                                                                                          <  2:797698/151‑1 (MQ=255)                           ccGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCtt                                                                                                                   <  1:797420/151‑1 (MQ=255)                             ggTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCt                                                                                                                    >  1:485722/1‑148 (MQ=255)                                aCGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTaa                                                                                                              <  1:479642/151‑1 (MQ=255)                                        cGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCggg                                                                                                      >  2:173772/1‑151 (MQ=255)                                        cGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCgg                                                                                                       <  2:585195/150‑1 (MQ=255)                                            gTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGataa                                                                                                  <  2:153551/151‑1 (MQ=255)                                             tAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGataat                                                                                                 >  2:551448/1‑151 (MQ=255)                                                   cacTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGtt                                                                                           <  1:907052/151‑1 (MQ=255)                                                    acTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTtc                                                                                          <  2:412855/151‑1 (MQ=255)                                                       gAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTtcatc                                                                                       <  1:702032/151‑1 (MQ=255)                                                       gAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTtcatc                                                                                       >  1:915593/1‑151 (MQ=255)                                                       gAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTtcat                                                                                        <  2:167121/150‑1 (MQ=255)                                                           gtgGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGtt                                                                                   <  1:256396/151‑1 (MQ=255)                                                            tgGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGtt                                                                                   <  2:137567/150‑1 (MQ=255)                                                             ggCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTc                                                                                  <  2:328903/150‑1 (MQ=255)                                                               ccagccagccaAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTaa                                                                                                                          >  2:993434/1‑108 (MQ=255)                                                               ccagccagccaAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTaa                                                                                                                          <  1:993475/108‑1 (MQ=255)                                                                cagccagccaAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTcgcg                                                                               >  2:87879/1‑150 (MQ=255)                                                                 agccagccaAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGAt                                                                             <  2:656270/151‑1 (MQ=255)                                                                     agccaAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGc                                                                         >  1:659351/1‑151 (MQ=255)                                                                           aGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCttt                                                                   <  2:1057506/151‑1 (MQ=255)                                                                            gCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTa                                                                  <  2:967756/151‑1 (MQ=255)                                                                               ttCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGc                                                                <  2:1045689/150‑1 (MQ=255)                                                                                 ccAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCAtt                                                             <  1:75102/151‑1 (MQ=255)                                                                                 ccAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCAtt                                                             <  2:12032/151‑1 (MQ=255)                                                                                     atgatTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGc                                                         <  1:1866/151‑1 (MQ=255)                                                                                       gatTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCgg                                                       <  2:979445/151‑1 (MQ=255)                                                                                       gatTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCgg                                                       <  2:238511/151‑1 (MQ=255)                                                                                       gatTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCgg                                                       <  2:415778/151‑1 (MQ=255)                                                                                          tcattcCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGAt                                                     >  2:407652/1‑150 (MQ=255)                                                                                           cattcCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACTGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATaa                                                   <  2:90199/151‑1 (MQ=255)                                                                                           cattcCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATaa                                                   <  1:595784/151‑1 (MQ=255)                                                                                              tcCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATaaaa                                                 <  2:21251/150‑1 (MQ=255)                                                                                               ccGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATaaa                                                  >  2:655511/1‑148 (MQ=255)                                                                                                cGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGAc                                              <  1:88000/151‑1 (MQ=255)                                                                                                 ggAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACt                                             >  2:15094/1‑151 (MQ=255)                                                                                                  gAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACtt                                            <  1:300540/151‑1 (MQ=255)                                                                                                  gAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACtt                                            >  2:670125/1‑151 (MQ=255)                                                                                                   aaaCCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACttt                                           <  2:327438/151‑1 (MQ=255)                                                                                                    aaCCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTa                                          <  1:175661/151‑1 (MQ=255)                                                                                                     aCCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTAc                                         <  2:354726/151‑1 (MQ=255)                                                                                                     aCCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTAc                                         <  2:969246/151‑1 (MQ=255)                                                                                                     aCCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTAc                                         <  1:1055942/151‑1 (MQ=255)                                                                                                       cAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGt                                       <  2:395769/151‑1 (MQ=255)                                                                                                       cAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGt                                       <  1:1044526/151‑1 (MQ=255)                                                                                                          cgcCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCtt                                    <  2:921992/151‑1 (MQ=255)                                                                                                          cgcCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCtt                                    <  1:924265/151‑1 (MQ=255)                                                                                                           gcCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCttt                                   <  1:278173/151‑1 (MQ=255)                                                                                                                  aCCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAgg                            <  1:656383/151‑1 (MQ=255)                                                                                                                  aCCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAg                             <  2:734748/150‑1 (MQ=255)                                                                                                                   cccGCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGc                           <  1:457587/151‑1 (MQ=255)                                                                                                                      gCCTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACg                        >  2:183821/1‑151 (MQ=255)                                                                                                                       ccTTGTGAAAGCCCTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGa                       <  1:155040/151‑1 (MQ=255)                                                                                                                                  cccTGCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCggg             >  2:934752/1‑150 (MQ=255)                                                                                                                                      gCCAGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGaata         <  2:372867/150‑1 (MQ=255)                                                                                                                                         aGCGCGCGGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGc     <  1:1043441/151‑1 (MQ=255)                                                                                                                                            gcgcgGCTATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCt  <  1:292765/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                GTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGCCATCGGCATCTTTACGGACATTAAAACGCTTGCTAATCAGCGGGATAATATCGTTCATCAGTTCGCGATCTGCCGCTTTAGCATTCAGCGGATAAAAGACTTTACGTCTTTCCTGAGGCACGAAATCTTCGGGAATAATGCCCT  >  NC_000913/3899618‑3899907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑