Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 4,270,398 | A→G | N54S (AAC→AGC) | yjbQ → | thiamine phosphate synthase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,270,398 | 0 | A | G | 98.4% | 226.4 / NA | 62 | N54S (AAC→AGC) | yjbQ | thiamine phosphate synthase |
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (32/29); minor base T (0/1); total (32/30) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.84e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AGACGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAACTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCC > NC_000913/4270251‑4270545 | aGACGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTg < 1:185800/151‑1 (MQ=255) gACGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGc < 2:142081/151‑1 (MQ=255) cGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGa < 1:97664/151‑1 (MQ=255) cGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGa < 2:161629/151‑1 (MQ=255) tCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTAAATGAGAGCTGCGATcc > 1:333395/1‑151 (MQ=255) cACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATc < 2:826128/149‑1 (MQ=255) cGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCAc > 1:119500/1‑151 (MQ=255) tAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGt < 2:447928/150‑1 (MQ=255) tAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATAGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCg > 2:121174/1‑149 (MQ=255) cgcCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGc > 1:464843/1‑151 (MQ=255) gcCAAATCACGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGcc < 1:150172/151‑1 (MQ=255) aaaTCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACg < 1:1047523/151‑1 (MQ=255) cGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATg < 1:330240/150‑1 (MQ=255) tCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGtttt > 2:867055/1‑151 (MQ=255) tCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGtttt > 1:102671/1‑151 (MQ=255) aTCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGtttttt > 2:769325/1‑151 (MQ=255) tCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTtc > 1:513074/1‑151 (MQ=255) ggTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCCCCGTACGCCACGACATGGAGCGTTTTTtc < 2:282055/148‑1 (MQ=255) ggATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCAcc < 2:1044087/151‑1 (MQ=255) ggATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCAcc > 2:589609/1‑151 (MQ=255) ggATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCAcc > 1:876088/1‑151 (MQ=255) aTGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGt > 1:850264/1‑151 (MQ=255) gAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGtt < 1:345720/150‑1 (MQ=255) tCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGAc > 1:1040856/1‑151 (MQ=255) tGAATCAGCTGGCTGATATGCCGCGCGTTAACATCTGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGAc > 1:873848/1‑149 (MQ=255) tGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACa > 2:792836/1‑150 (MQ=255) gAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACaa < 1:580085/150‑1 (MQ=255) tCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACgg > 2:360799/1‑150 (MQ=255) cAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGa < 1:412480/150‑1 (MQ=255) ctggctgATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAAt < 2:935581/150‑1 (MQ=255) atGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCAt < 1:187606/150‑1 (MQ=255) atGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCAt < 2:457936/150‑1 (MQ=255) gCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGa < 2:651133/150‑1 (MQ=255) cgcgcgTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTa < 1:495130/151‑1 (MQ=255) gcgTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATg > 1:662914/1‑150 (MQ=255) cgTTAACATCGGCTTACTGCATCTATCGTTGCAACAGGCCCCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCTCCGTACGCCACGACAAGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGa < 2:15437/150‑1 (MQ=255) gTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAgg > 2:102596/1‑151 (MQ=255) aaCATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGa < 2:119496/150‑1 (MQ=255) aTCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGc > 2:792983/1‑149 (MQ=255) tACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGatat > 2:487086/1‑151 (MQ=255) gCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGcc < 1:272920/150‑1 (MQ=255) gCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCt < 1:647006/151‑1 (MQ=255) tGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTGCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCtt < 1:551830/147‑1 (MQ=255) ttGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCat > 2:1026467/1‑150 (MQ=255) ctgcAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCat < 2:242591/147‑1 (MQ=255) ctgcAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATc > 1:846037/1‑150 (MQ=255) gcAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCaa > 1:112182/1‑150 (MQ=255) gcAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCaa > 2:489304/1‑150 (MQ=255) cAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAAt > 2:153597/1‑151 (MQ=255) cAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAAt > 1:907556/1‑151 (MQ=255) aCATACCTCCGCCTCTCTGACGCTTAATGAGATCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCAACGTTCCCGACAACGTAAATTATGAGCATGACTATGAGGGAGCACACGATATGCCTTCTCATATCAAAt < 2:439422/149‑1 (MQ=255) aCCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCa < 1:289478/150‑1 (MQ=255) cTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATg > 2:52933/1‑151 (MQ=255) tCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGc > 2:745141/1‑151 (MQ=255) tctctGACACTTAATGAGAGCTGCGATCCCACTGGACGCCACACCATGGAGCGTTATTTCCTCCGCACCGTTCACGAAAACGGAAATTATGAGAATGACTATGAGAGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGa > 1:435106/1‑150 (MQ=255) tctGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAAc > 1:652840/1‑150 (MQ=255) tctGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAAc > 2:790990/1‑150 (MQ=255) ctGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGaa < 2:41322/148‑1 (MQ=255) gACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACa < 2:137197/148‑1 (MQ=255) cacTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAg < 2:582385/86‑1 (MQ=255) cacTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAg > 1:582401/1‑86 (MQ=255) gagaGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGc > 2:85850/1‑151 (MQ=255) agagCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGcc > 2:345635/1‑151 (MQ=255) | AGACGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAACTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCC > NC_000913/4270251‑4270545 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |