Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,431,878	T→C	T36A (ACG→GCG)	ytfE ←	iron‑sulfur cluster repair protein RIC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,431,878	0	T	C	100.0%	228.3 / NA	65	T36A (ACG→GCG)	ytfE	iron‑sulfur cluster repair protein RIC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/38);  total (27/38)

CGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAA  >  NC_000913/4431731‑4432023                                                                                                                                                    |                                                                                                                                                  cGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCt                                                                                                                                                 <  1:1023372/150‑1 (MQ=255)        gcgcACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTAcct                                                                                                                                         <  2:36359/151‑2 (MQ=255)           cACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGcc                                                                                                                                       >  2:1041730/1‑150 (MQ=255)                tgatATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCAcagc                                                                                                                                  >  2:493866/1‑150 (MQ=255)                  atatGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGt                                                                                                                               >  1:441668/1‑151 (MQ=255)                  atatGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCCCAACAGCCATTTCTTTACTTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGt                                                                                                                               <  1:97870/151‑1 (MQ=255)                    atGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCTTGCTTACCGCCACAGCAGTa                                                                                                                              <  1:541386/150‑1 (MQ=255)                     tGGTCGATGATTTCTGCCAGCGGGGCGCTCCGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGTTTGGCCAGTTCAGCTTCAATGCCCTCACCATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCAcagcag                                                                                                                                <  2:447647/147‑1 (MQ=255)                          gatgatTCCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTCCAGCGAGTTTTGCCAGTTCAGTTTCAAGGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCTTCCTTACCGCCACAGCAGTAATCca                                                                                                                         <  2:453778/149‑1 (MQ=255)                           atgatTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCcatat                                                                                                                      <  1:198419/151‑1 (MQ=255)                            tgatTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCcatat                                                                                                                      <  1:359585/150‑1 (MQ=255)                                 tCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCcatatcatat                                                                                                                 <  1:181174/150‑1 (MQ=255)                                   tGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATAttt                                                                                                               >  1:20846/1‑150 (MQ=255)                                    gCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTa                                                                                                              <  1:1023786/150‑1 (MQ=255)                                    gCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTAc                                                                                                             <  1:173881/151‑1 (MQ=255)                                     ccAGCGGGGCGCTCCGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCCCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTAc                                                                                                             <  2:537847/150‑1 (MQ=255)                                      cAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACg                                                                                                            >  2:312842/1‑150 (MQ=255)                                       aGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGa                                                                                                           >  1:554903/1‑150 (MQ=255)                                          ggggCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAAc                                                                                                        <  2:249508/150‑1 (MQ=255)                                            ggCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGCCCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACaga                                                                                                     <  2:350828/151‑1 (MQ=255)                                             gcgcTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCATTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACagag                                                                                                    <  2:733383/151‑1 (MQ=255)                                                 tACGCCAGTCTTTCTCAATCGGTTGGTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTg                                                                                                 >  2:457206/1‑150 (MQ=255)                                                  aCGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTg                                                                                                 <  2:950023/149‑1 (MQ=255)                                                  aCGCCAGTCTTTCCCAATCGGTTGTTCAGCGAGCTTTGCCAGTTGAGCTTCAATGCCCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCGGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGa                                                                                                <  2:239026/150‑1 (MQ=255)                                                      cAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAgcgc                                                                                           >  1:622456/1‑151 (MQ=255)                                                        gTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAgcgc                                                                                           <  1:12321/149‑1 (MQ=255)                                                           tttCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAAc                                                                                                        >  2:346469/1‑133 (MQ=255)                                                           tttCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAAc                                                                                                        <  1:346480/133‑1 (MQ=255)                                                            ttCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAgg                                                                                       <  1:703308/149‑1 (MQ=255)                                                              ctcAACCGTTGGTTCCGCGCGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCTCGCGGTCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGa                                                                                         <  2:489526/145‑1 (MQ=255)                                                               tcAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAAt                                                                                    >  1:763884/1‑149 (MQ=255)                                                                cAATCGGTTTTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAAt                                                                                    <  2:203279/148‑1 (MQ=255)                                                                cAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATa                                                                                   <  1:692586/149‑1 (MQ=255)                                                                    cGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATagagag                                                                              <  2:816278/150‑1 (MQ=255)                                                                     ggTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATagagag                                                                              <  2:477345/149‑1 (MQ=255)                                                                         gttCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGcc                                                                          >  1:1012676/1‑149 (MQ=255)                                                                          ttCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGt                                                                       >  2:20137/1‑151 (MQ=255)                                                                                    tttGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTaa                                                               >  2:442615/1‑149 (MQ=255)                                                                                    tttGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCCCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCAc                                                                   <  2:166162/145‑1 (MQ=255)                                                                                           gTTCAGCTTCAATGCCCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGt                                                       <  2:618760/150‑1 (MQ=255)                                                                                                   tCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATa                                                >  2:79997/1‑149 (MQ=255)                                                                                                    cAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAg                                              <  1:956945/150‑1 (MQ=255)                                                                                                     aaTGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGcc                                            >  2:746324/1‑151 (MQ=255)                                                                                                      aTGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGcc                                            >  2:517142/1‑150 (MQ=255)                                                                                                       tGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCa                                           >  2:43672/1‑150 (MQ=255)                                                                                                          ccTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGGCGCGATAAGCCATAGc                                       >  2:412120/1‑151 (MQ=255)                                                                                                           cTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCt                                      >  1:177401/1‑151 (MQ=255)                                                                                                            tCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTg                                     >  2:714674/1‑151 (MQ=255)                                                                                                             cAACATCCATTTCTTTACGTGCCGCCGCGCGCGCCGGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGc                                       <  2:983442/148‑1 (MQ=255)                                                                                                             cAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTg                                     >  2:946824/1‑150 (MQ=255)                                                                                                                cATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATAc                                 <  2:729102/151‑1 (MQ=255)                                                                                                                 aTCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATAcc                                >  1:601934/1‑151 (MQ=255)                                                                                                                   ccAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTc                              <  1:793213/151‑1 (MQ=255)                                                                                                                   ccAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTc                              <  1:936940/151‑1 (MQ=255)                                                                                                                       ttCTTTGCGTGCCGCCGCGCGCACCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCAtt                           <  2:244267/150‑1 (MQ=255)                                                                                                                        tCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTc                          <  1:320463/150‑1 (MQ=255)                                                                                                                         cTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCtt                        >  2:920194/1‑151 (MQ=255)                                                                                                                          tttACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCtt                        >  2:379937/1‑150 (MQ=255)                                                                                                                             aCGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCGAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTtaa                      <  1:334905/149‑1 (MQ=255)                                                                                                                               gTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTtaata                    >  1:852419/1‑149 (MQ=255)                                                                                                                                    gccgcGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAataagata               >  1:345242/1‑149 (MQ=255)                                                                                                                                    gccgcGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAataagata               >  1:522963/1‑149 (MQ=255)                                                                                                                                     ccgcGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAataagataag             <  2:87087/150‑1 (MQ=255)                                                                                                                                            cgcCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTAtt      <  2:295687/150‑1 (MQ=255)                                                                                                                                               cAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTaa  <  1:424970/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  CGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAA  >  NC_000913/4431731‑4432023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑