Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	304,787	G→A	P140L (CCG→CTG)	ecpE ←	ECP production pilus chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	304,787	0	G	A	100.0%	228.8 / NA	58	P140L (CCG→CTG)	ecpE	ECP production pilus chaperone
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/20);  total (38/20)

CTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTG  >  NC_000913/304639‑304932                                                                                                                                                     |                                                                                                                                                  ctctTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGc                                                                                                                                                 >  2:622402/1‑151 (MQ=255)    ttCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTcc                                                                                                                                              >  2:378867/1‑151 (MQ=255)     tCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTc                                                                                                                                               >  1:178888/1‑149 (MQ=255)     tCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTc                                                                                                                                               <  1:311785/149‑1 (MQ=255)      cGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGt                                                                                                                                            >  1:190898/1‑151 (MQ=255)       ggTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTg                                                                                                                                           <  2:700250/151‑1 (MQ=255)          cGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCt                                                                                                                                         <  1:622419/150‑1 (MQ=255)            aaTCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGAc                                                                                                                                      >  1:392388/1‑151 (MQ=255)                  aTCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCAcgcc                                                                                                                                <  2:921305/151‑1 (MQ=255)                   tCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCAcgccg                                                                                                                               >  2:470388/1‑151 (MQ=255)                   tCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCAcgccg                                                                                                                               >  2:455854/1‑151 (MQ=255)                         cTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGtt                                                                                                                         >  1:478922/1‑151 (MQ=255)                              tCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCt                                                                                                                    >  1:125661/1‑151 (MQ=255)                                  tAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGCCACCTTGTCGAAGGACCATTTAACCTGAACCTGACGTGGTCGCACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTCCTGACCACGCCGCCGGTTGGGCTGCGt                                                                                                                >  2:980798/1‑151 (MQ=255)                                    gCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCt                                                                                                              <  1:199049/151‑1 (MQ=255)                                    gCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCt                                                                                                              >  2:566305/1‑151 (MQ=255)                                     cTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCtt                                                                                                             >  2:463463/1‑151 (MQ=255)                                      ttAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCtt                                                                                                             >  2:424624/1‑150 (MQ=255)                                         aaCCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTAt                                                                                                         <  2:52820/151‑1 (MQ=255)                                          aCCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATg                                                                                                        <  1:1060989/151‑1 (MQ=255)                                           ccATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATgg                                                                                                       >  1:849377/1‑151 (MQ=255)                                           ccATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATgg                                                                                                       >  1:20012/1‑151 (MQ=255)                                                   tGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGt                                                                                               >  2:778746/1‑151 (MQ=255)                                                    gCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTg                                                                                              >  1:219478/1‑151 (MQ=255)                                                          gtTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAcc                                                                                        <  1:96205/151‑1 (MQ=255)                                                            tacttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTc                                                                                      >  2:164048/1‑151 (MQ=255)                                                             acttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTcg                                                                                     >  2:674169/1‑151 (MQ=255)                                                             acttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTcg                                                                                     >  2:257227/1‑151 (MQ=255)                                                             acttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTc                                                                                      <  1:340571/150‑1 (MQ=255)                                                             acttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTc                                                                                      <  1:18507/150‑1 (MQ=255)                                                               ttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTcgcg                                                                                   >  2:396506/1‑151 (MQ=255)                                                               ttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTcgcg                                                                                   >  2:927598/1‑151 (MQ=255)                                                               ttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTcgcg                                                                                   >  2:344226/1‑151 (MQ=255)                                                                 acCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGaa                                                                                 <  1:126250/151‑1 (MQ=255)                                                                 acCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGa                                                                                  <  2:443357/150‑1 (MQ=255)                                                                    gTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATg                                                                              >  1:313946/1‑151 (MQ=255)                                                                      tCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATga                                                                             <  1:390569/150‑1 (MQ=255)                                                                        cTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGAc                                                                          >  2:289824/1‑151 (MQ=255)                                                                             aCCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGgt                                                                     >  2:213894/1‑151 (MQ=255)                                                                                   tCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAgc                                                               >  2:216640/1‑151 (MQ=255)                                                                                   tCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAgc                                                               >  2:626460/1‑151 (MQ=255)                                                                                     gAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAgcgc                                                             <  2:125031/151‑1 (MQ=255)                                                                                        ggACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTcg                                                          >  2:647886/1‑151 (MQ=255)                                                                                         gACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTcgc                                                         >  1:380236/1‑151 (MQ=255)                                                                                                       aaCCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTgg                                            >  2:894789/1‑150 (MQ=255)                                                                                                         ccTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGAcc                                         <  1:358171/151‑1 (MQ=255)                                                                                                          cTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGAcc                                         >  1:915091/1‑150 (MQ=255)                                                                                                           tGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCAt                                       >  1:532760/1‑151 (MQ=255)                                                                                                            gACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCAt                                       <  1:576443/150‑1 (MQ=255)                                                                                                                  gTCGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTa                                <  2:408476/151‑1 (MQ=255)                                                                                                                    cGTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTaa                               >  2:665750/1‑150 (MQ=255)                                                                                                                     gTACTACCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTaaa                              >  1:462447/1‑150 (MQ=255)                                                                                                                      tactacCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGCAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAAt                            >  2:243100/1‑151 (MQ=255)                                                                                                                          acCAGAATGGTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAAttt                          <  2:1038334/149‑1 (MQ=255)                                                                                                                                  ggTATCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCg                >  1:560506/1‑151 (MQ=255)                                                                                                                                     aTCCATCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGct              <  2:602242/150‑1 (MQ=255)                                                                                                                                          tCACCACCACCAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCAcc        >  1:419650/1‑151 (MQ=255)                                                                                                                                            accaccaccAGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAg      <  2:786053/151‑1 (MQ=255)                                                                                                                                                ccaccaGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTg  <  2:885404/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  CTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTG  >  NC_000913/304639‑304932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑