Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	368,846	A→G	H79R (CAC→CGC)	mhpA →	3‑(3‑hydroxyphenyl)propionate hydroxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	368,846	0	A	G	100.0%	168.7 / NA	45	H79R (CAC→CGC)	mhpA	3‑(3‑hydroxyphenyl)propionate hydroxylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/30);  total (15/30)

GAACTATCTCGGCCAGATGGGCATTGACGTGCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGT  >  NC_000913/368703‑368992                                                                                                                                                |                                                                                                                                                   gAACTATCTCGGCCAGATGGGCATTGACGTGCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACg                                                                                                                                             <  2:667849/151‑1 (MQ=255)          cGGCCAGATGGGCATTGACGTGCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTgg                                                                                                                                       <  2:804818/148‑1 (MQ=255)          cGGCCAGATGGGCATTGACGTGCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGc                                                                                                                                      <  2:698191/149‑1 (MQ=255)          cGGCCAGATGGGCATTGACGTGCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCa                                                                                                                                     <  2:855248/150‑1 (MQ=255)           ggCCAGATGGGCATTGACGTGCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGCATTGATGACGAGGCGCTGCGCACCATGCAGTAGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCAcg                                                                                                                                   >  2:792849/1‑151 (MQ=255)              cAGATGGGCATTGACGTGCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCAcgcg                                                                                                                                 >  1:699288/1‑150 (MQ=255)               aGATGGGCATTGACGTGCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGAt                                                                                                                               >  1:759118/1‑151 (MQ=255)                   gggCATTGACGTGCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGt                                                                                                                           <  2:1001378/151‑1 (MQ=255)                                 tggtggtggAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGa                                                                                                              >  2:173527/1‑150 (MQ=255)                                 tggtggtggAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGCGGCACGCGATGCGTATTCTCACCCCGa                                                                                                              >  1:522042/1‑150 (MQ=255)                                     ggtggAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAgg                                                                                                          <  1:174182/150‑1 (MQ=255)                                        ggAGAAACTCGATAAGTTGATCGACTCCCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCg                                                                                                       <  1:253343/150‑1 (MQ=255)                                        ggAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCg                                                                                                       <  2:878884/150‑1 (MQ=255)                                           gaAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGt                                                                                                   <  2:999956/151‑1 (MQ=255)                                                 cGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCt                                                                                             <  2:886487/151‑1 (MQ=255)                                                  gATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTg                                                                                            <  1:829221/151‑1 (MQ=255)                                                   aTAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTg                                                                                            >  2:297902/1‑150 (MQ=255)                                                    tAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGat                                                                                          >  1:344507/1‑151 (MQ=255)                                                    tAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGat                                                                                          >  2:1011218/1‑151 (MQ=255)                                                         tGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTc                                                                                      <  1:144431/150‑1 (MQ=255)                                                             cGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCa                                                                                 <  2:136707/151‑1 (MQ=255)                                                              gACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCa                                                                                 >  2:202143/1‑150 (MQ=255)                                                               aCTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAAt                                                                               <  1:435024/151‑1 (MQ=255)                                                                   cccGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGAcc                                                                           >  1:915127/1‑151 (MQ=255)                                                                     cgcgTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGa                                                                         <  1:423031/151‑1 (MQ=255)                                                                      gcgTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGAt                                                                        <  1:668746/151‑1 (MQ=255)                                                                          gCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAAt                                                                    >  2:478711/1‑151 (MQ=255)                                                                             aTTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTtg                                                                 <  2:651765/151‑1 (MQ=255)                                                                                  tATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTtggctgg                                                            >  2:22589/1‑151 (MQ=255)                                                                                  tATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTtggctgg                                                            >  2:691750/1‑151 (MQ=255)                                                                                      gatgaCGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTTGCTGGcct                                                         <  2:330332/150‑2 (MQ=255)                                                                                            gAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTa                                                  <  2:124401/151‑1 (MQ=255)                                                                                                cgcTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGc                                              >  1:268264/1‑151 (MQ=255)                                                                                                     cgcACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTa                                         <  2:306410/151‑1 (MQ=255)                                                                                                       cACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTAtt                                       >  2:859574/1‑151 (MQ=255)                                                                                                                      cggCCTGGTCGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGAt                        <  2:915091/151‑1 (MQ=255)                                                                                                                            ggTCGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCgg                    <  1:381816/149‑1 (MQ=255)                                                                                                                             gTCGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGa                 <  2:16377/151‑1 (MQ=255)                                                                                                                               cGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGAt                <  2:4421/150‑1 (MQ=255)                                                                                                                               cGATGATGTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATg               <  2:68312/151‑1 (MQ=255)                                                                                                                                   gatgTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTgg           <  1:886240/151‑1 (MQ=255)                                                                                                                                      gTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGaa         <  1:471760/150‑1 (MQ=255)                                                                                                                                      gTTCTGCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAg        <  2:613734/151‑1 (MQ=255)                                                                                                                                           gCCGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGgt    <  2:16799/150‑1 (MQ=255)                                                                                                                                            ccGCGCACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGgtgt  <  1:769937/151‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                   GAACTATCTCGGCCAGATGGGCATTGACGTGCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGT  >  NC_000913/368703‑368992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑