Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	624,671	A→G	intergenic (‑161/‑214)	fepB ← / → entC	ferrienterobactin ABC transporter periplasmic binding protein/isochorismate synthase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	624,671	0	A	G	100.0%	123.9 / NA	35	intergenic (‑161/‑214)	fepB/entC	ferrienterobactin ABC transporter periplasmic binding protein/isochorismate synthase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/7);  total (28/7)

TTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAA‑CAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTT  >  NC_000913/624528‑624817                                                                                                                                                 |                                                                                                                                                   ttaATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAA‑CAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAagggggaggggg                                                                                                                                             >  1:704709/1‑151 (MQ=255)   aataaGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAA‑CAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGc                                                                                                                                            >  2:140382/1‑150 (MQ=255)          ttAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAA‑CAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGgc                                                                                                                                    >  1:731360/1‑151 (MQ=255)                   tAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAA‑CAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACAt                                                                                                                           >  1:2420/1‑151 (MQ=255)                      tGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAA‑CAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATcac                                                                                                                        >  1:680963/1‑151 (MQ=255)                         cAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAA‑CAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACAtt                                                                                                                     >  2:44005/1‑151 (MQ=255)                          aaaTTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAA‑CAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTg                                                                                                                    >  1:1018491/1‑151 (MQ=255)                          aaaTTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAA‑CAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTg                                                                                                                    >  1:814185/1‑151 (MQ=255)                          aaaTTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAA‑CAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTg                                                                                                                    >  1:844985/1‑151 (MQ=255)                             ttCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGCGAGGGGAA‑CAGCCAGCACTGGAGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTgcg                                                                                                                  >  1:694923/1‑150 (MQ=255)                                          acaATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAA‑CAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATcc                                                                                                     >  2:1023340/1‑150 (MQ=255)                                              tCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGCGAGGGGAA‑CAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCa                                                                                                 >  2:714968/1‑150 (MQ=255)                                                cGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAA‑CAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCaat                                                                                               >  2:835586/1‑150 (MQ=255)                                                           cgtgggggggggggggGTGGGGGGGGGGGAACCAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTtct                                                                                      <  1:573229/143‑1 (MQ=255)                                                                gggggAGGGGGGTGGGGGGGGGGCCC‑CAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCa                                                                              <  1:955399/150‑1 (MQ=255)                                                                 ggggAGAGGGTTAGGGTGAGGGGAA‑CAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCAt                                                                             >  2:497626/1‑151 (MQ=255)                                                                    gagaGGGTTAGGGTGAGGGGAA‑CAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGt                                                                          >  1:72104/1‑151 (MQ=255)                                                                                gTGAGGGGAA‑CAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCAt                                                                             <  1:649851/136‑1 (MQ=255)                                                                                gTGAGGGGAA‑CAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCAt                                                                             >  2:649833/1‑136 (MQ=255)                                                                                gTGAGGGGAA‑CAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAAcc                                                                >  1:989629/1‑149 (MQ=255)                                                                                  ggggccccccagccagcACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCa                                                               <  2:238359/140‑1 (MQ=255)                                                                                  gAGGGGAA‑CAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAAcc                                                                >  1:165353/1‑147 (MQ=255)                                                                                        aa‑CAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAAcc                                                      >  2:285376/1‑151 (MQ=255)                                                                                          ccagccagcACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATg                                                           <  1:84265/144‑1 (MQ=255)                                                                                              ccagcACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCg                                                   >  1:242863/1‑149 (MQ=255)                                                                                                   aCTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATTAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGc                                             <  1:375183/150‑1 (MQ=255)                                                                                                    cTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGc                                             >  2:197322/1‑149 (MQ=255)                                                                                                                     ccTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCa                             >  2:674111/1‑148 (MQ=255)                                                                                                                        cACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGtt                       >  1:470416/1‑151 (MQ=255)                                                                                                                            ccAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGAc                    >  2:941469/1‑150 (MQ=255)                                                                                                                             cAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACAt                  >  2:1024074/1‑151 (MQ=255)                                                                                                                                      cccTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGt         >  1:656422/1‑151 (MQ=255)                                                                                                                                         tGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTg      >  2:1050835/1‑151 (MQ=255)                                                                                                                                          ggAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTg      <  2:756523/150‑1 (MQ=255)                                                                                                                                            aaGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCtt   >  2:143743/1‑151 (MQ=255)                                                                                                                                             aGGGGGGGGGGGCAGAAGGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCttt  >  2:273618/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                   TTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAA‑CAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTT  >  NC_000913/624528‑624817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑