Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	649,250	C→T	V108I (GTA→ATA)	citF ←	citrate lyase, citrate‑ACP transferase (alpha) subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	649,250	0	C	T	100.0%	212.5 / NA	55	V108I (GTA→ATA)	citF	citrate lyase, citrate‑ACP transferase (alpha) subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/32);  total (23/32)

CCGCTCTGTACCAGATGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCT  >  NC_000913/649105‑649393                                                                                                                                                  |                                                                                                                                                ccgcTCTGTCCCAGTTGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAgcg                                                                                                                                            <  1:202672/151‑1 (MQ=255)   gcTCTGTACCAAAAGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAgcggc                                                                                                                                          <  1:680880/151‑1 (MQ=255)    ctctGTACCAGATGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAgcggcg                                                                                                                                         >  1:56642/1‑151 (MQ=255)     tctGTACCAGATGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGgcgc                                                                                                                                        >  1:590091/1‑151 (MQ=255)          aCCAGATGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGc                                                                                                                                   >  1:245636/1‑151 (MQ=255)           ccAGATGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATgg                                                                                                                                    <  1:766682/149‑1 (MQ=255)              gATGCACACGCCCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCa                                                                                                                                  <  2:241152/148‑1 (MQ=255)                  cacaCGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCa                                                                                                                              <  1:889204/148‑1 (MQ=255)                  cacaCGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCAc                                                                                                                             <  2:78261/149‑1 (MQ=255)                     acGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAgg                                                                                                                        >  2:187800/1‑151 (MQ=255)                      gggcggccgAGAGAGTGGATCAGCACCGTTTCGGCCTGCAGACCACGGGAGATTTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAAGCTCGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAg                                                                                                                         <  2:554568/144‑1 (MQ=255)                         ccgccgTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGa                                                                                                                      <  2:318965/149‑1 (MQ=255)                            ccgTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTgg                                                                                                                 <  2:398571/151‑1 (MQ=255)                              gTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGAc                                                                                                               <  1:234690/151‑1 (MQ=255)                               tGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACg                                                                                                              >  1:458906/1‑151 (MQ=255)                                gagaGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACg                                                                                                              <  1:671234/150‑1 (MQ=255)                                      ggATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAggg                                                                                                        >  2:1044769/1‑150 (MQ=255)                                      ggATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGt                                                                                                       >  2:18583/1‑151 (MQ=255)                                      ggATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGt                                                                                                       >  2:457926/1‑151 (MQ=255)                                             cACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGttt                                                                                                >  2:123640/1‑151 (MQ=255)                                               ccGGTTCTGCCAGCCGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGCATACATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGttttt                                                                                              >  2:133921/1‑151 (MQ=255)                                               ccGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGttttt                                                                                              >  1:108315/1‑151 (MQ=255)                                                 ggTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTaa                                                                                            <  2:69717/151‑1 (MQ=255)                                                  gTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTaaa                                                                                           <  2:382898/151‑1 (MQ=255)                                                  gTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTaa                                                                                            >  1:670583/1‑150 (MQ=255)                                                    tCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAg                                                                                          <  1:39344/150‑1 (MQ=255)                                                          agcagACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCt                                                                                   <  2:566792/151‑1 (MQ=255)                                                            cagACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTc                                                                                 <  2:694183/151‑1 (MQ=255)                                                              gACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTcgc                                                                               <  1:437510/151‑1 (MQ=255)                                                               aCCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTcgcg                                                                              >  2:374315/1‑151 (MQ=255)                                                                ccACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGa                                                                             <  2:173916/151‑1 (MQ=255)                                                                  aCGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTGTCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATg                                                                           <  1:184272/151‑1 (MQ=255)                                                                  aCGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATg                                                                           <  1:285657/151‑1 (MQ=255)                                                                             ctTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGG‑TTTTAAAGCCCATCTTCGCGATGACGTCCATc                                                                  <  2:413428/148‑1 (MQ=255)                                                                                ccGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCat                                                             >  1:20147/1‑151 (MQ=255)                                                                                   ccAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATAtt                                                          <  2:376379/151‑1 (MQ=255)                                                                                    cAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTg                                                         >  1:602561/1‑151 (MQ=255)                                                                                      gTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGAc                                                       >  2:472941/1‑151 (MQ=255)                                                                                        ggACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACg                                                      <  2:411680/150‑1 (MQ=255)                                                                                              cGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACggtcaggt                                               <  2:481241/151‑1 (MQ=255)                                                                                              cGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACggtcaggt                                               >  1:1059544/1‑151 (MQ=255)                                                                                                   gCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCg                                          >  1:982145/1‑151 (MQ=255)                                                                                                     cGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGcc                                        >  1:779899/1‑151 (MQ=255)                                                                                                         ggTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCAGGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCCCCATATTGACGGTCAGGTCACCGCCACg                                     <  2:62614/150‑1 (MQ=255)                                                                                                          gTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGa                                   <  1:901422/151‑1 (MQ=255)                                                                                                            ataAATGCGGGTACCCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGaa                                  <  2:771555/150‑1 (MQ=255)                                                                                                              aaaTGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGATCACCGCCACGGAAAg                                <  2:1019504/150‑1 (MQ=255)                                                                                                                  gCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTg                            <  1:261214/150‑1 (MQ=255)                                                                                                                        aaacACGCCCGGGCGAATGTGTTCTATCAGCGGCGAATGGCAACCACTCAGGGAGCTGGAGGCCAGGGTCAGGTTTTTAAAGCCCATCTCCGCGATGACGTCAATCAACAAATTGGCGGTCAGGTCGCCGCCACGGAAAGCGTGATggaag                     <  2:419019/148‑1 (MQ=255)                                                                                                                           cACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATggaaggaa                  <  2:72607/151‑1 (MQ=255)                                                                                                                                ccTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGt             <  2:393144/151‑1 (MQ=255)                                                                                                                                    gCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATg         >  2:303109/1‑151 (MQ=255)                                                                                                                                    gCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATg         >  1:422994/1‑151 (MQ=255)                                                                                                                                     cGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGc        >  2:460091/1‑151 (MQ=255)                                                                                                                                           tgtTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCt  >  1:546866/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                                CCGCTCTGTACCAGATGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCT  >  NC_000913/649105‑649393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑