Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	649,679	A→G	V271A (GTG→GCG)	citE ←	citrate lyase, citryl‑ACP lyase (beta) subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	649,679	0	A	G	100.0%	206.7 / NA	59	V271A (GTG→GCG)	citE	citrate lyase, citryl‑ACP lyase (beta) subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/27);  total (32/27)

ACCCGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTT  >  NC_000913/649531‑649826                                                                                                                                                     |                                                                                                                                                    aCCCGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACcgcg                                                                                                                                                   >  2:636665/1‑151 (MQ=255)    cGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCg                                                                                                                                                >  2:516597/1‑151 (MQ=255)    cGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCg                                                                                                                                                >  2:106462/1‑151 (MQ=255)    cGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCg                                                                                                                                                <  1:676594/151‑1 (MQ=255)     gTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCg                                                                                                                                                >  2:839/1‑150 (MQ=255)      ttCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCg                                                                                                                                                >  2:959602/1‑149 (MQ=255)        cTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGc                                                                                                                                            >  2:1022061/1‑151 (MQ=255)          tGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAgg                                                                                                                                             <  1:516612/148‑1 (MQ=255)          tGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGcc                                                                                                                                           <  1:328370/150‑1 (MQ=255)           gTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCtt                                                                                                                                         >  1:976281/1‑151 (MQ=255)           gTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCt                                                                                                                                          <  2:71694/150‑1 (MQ=255)                   gaTTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAgcg                                                                                                                                 >  2:255252/1‑151 (MQ=255)                          cAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAgcggccg                                                                                                                             >  1:221813/1‑146 (MQ=255)                            aTTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCa                                                                                                                        <  1:100643/151‑1 (MQ=255)                              tttCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAg                                                                                                                       <  2:944930/150‑1 (MQ=255)                                 cTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGc                                                                                                                   >  1:725993/1‑151 (MQ=255)                                   gCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCt                                                                                                                  <  2:664314/150‑1 (MQ=255)                                    cGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCCTCAGGGGCTTc                                                                                                                >  2:285360/1‑151 (MQ=255)                                     gTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCt                                                                                                               >  1:859753/1‑151 (MQ=255)                                            tGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTacgacg                                                                                                         >  2:22866/1‑150 (MQ=255)                                                 gacTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGcggcgg                                                                                                   >  2:539438/3‑151 (MQ=255)                                                  ccTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCggcggg                                                                                                  <  2:554570/151‑1 (MQ=255)                                                    ttATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCggcggg                                                                                                  >  2:53370/1‑149 (MQ=255)                                                    ttATTCTTCGCGGATGCCGGAAAGATCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCggcgggcg                                                                                                >  1:665047/1‑151 (MQ=255)                                                        tcttcGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTggt                                                                                            >  2:8412/1‑149 (MQ=255)                                                          ttcGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATcc                                                                                          >  1:502949/1‑151 (MQ=255)                                                           tcGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATcc                                                                                          >  2:121021/1‑150 (MQ=255)                                                             gcgGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCAct                                                                                       <  2:742269/151‑1 (MQ=255)                                                             gcgGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCAc                                                                                        >  2:804860/1‑150 (MQ=255)                                                               ggATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGGTCCActt                                                                                      >  1:267907/1‑150 (MQ=255)                                                                  tGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACttcttt                                                                                  >  2:320837/1‑151 (MQ=255)                                                                      ggAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTgg                                                                              >  1:793797/1‑151 (MQ=255)                                                                       gAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTggg                                                                             <  1:303119/151‑1 (MQ=255)                                                                                   cGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTaga                                                                 <  1:853173/151‑1 (MQ=255)                                                                                       agggCACCAGACGGTCGCGAGCGTTAATCGGAGCGTCCACCATGTGGCCGTAGAGGGAAACCGCGCTGAGGCCTGCGGGGGCTGCGGTTTCAGCGGCCTCTACGCCGCGGCGGGCGGGATCCACGTCTATCTGGGTCGGTGCGTAGAGGtt                                                             <  2:829308/148‑1 (MQ=255)                                                                                            aCCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGGGATCCCCTTCTTTCTGGGTCGGGGCGGAGAGGGTGTgc                                                         >  1:175840/1‑150 (MQ=255)                                                                                            aCCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTgca                                                        <  1:472953/151‑1 (MQ=255)                                                                                               aGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTgcagca                                                     <  1:460102/151‑1 (MQ=255)                                                                                                  cGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGa                                                   <  2:20147/150‑1 (MQ=255)                                                                                                  cGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGGGATCCACTTTTTTCTGGGGCGGTGGGTAGAGGGtggggaggaggt                                                  >  1:254029/1‑141 (MQ=255)                                                                                                   gggCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATc                                                 <  2:380345/151‑1 (MQ=255)                                                                                                       gcgATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATc                                             <  2:994581/151‑1 (MQ=255)                                                                                                          atcgatAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGa                                          <  1:390895/151‑1 (MQ=255)                                                                                                           tcgatAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATTCCCTTCTTTCTGGGTCGGGGCGTAGAGGGTGGGCGGCAGATCAATCt                                            >  2:656051/1‑148 (MQ=255)                                                                                                                aaCCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCggg                                    >  1:449750/1‑151 (MQ=255)                                                                                                                 aCCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTTATCCACTTCTTTCTGGGTCGGTGCGTAGAGGGTGTGGAGCAGATCAATCTGACGCggg                                    >  2:954063/1‑150 (MQ=255)                                                                                                                  ccGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGtt                                  <  2:140231/151‑1 (MQ=255)                                                                                                                  ccGGACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGt                                   <  2:314086/150‑1 (MQ=255)                                                                                                                    ggACCGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGa                                <  2:423054/151‑1 (MQ=255)                                                                                                                       ccGTCCACCATCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTTGGTCGGTGCGTAGAGGTTTTGCAGCAGATCAATCTGACGCGGGGTGAt                               >  2:612413/1‑149 (MQ=255)                                                                                                                           ccaccaTCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCg                          >  1:535935/1‑150 (MQ=255)                                                                                                                            caccaTCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGAt                        <  2:245627/151‑1 (MQ=255)                                                                                                                            caccaTCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGAt                        <  2:108312/151‑1 (MQ=255)                                                                                                                            caccaTCTTGCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGGGCGGAGAGGGTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCg                          >  2:367502/1‑149 (MQ=255)                                                                                                                                     gCCGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTc                <  2:469519/150‑1 (MQ=255)                                                                                                                                       cGTTCAGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGAGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCaa              <  2:670565/150‑1 (MQ=255)                                                                                                                                            aGGGAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCa        <  2:510587/151‑1 (MQ=255)                                                                                                                                               gAAACCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGGTCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGc      >  2:795780/1‑150 (MQ=255)                                                                                                                                                 aaCCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTTTGGGGCGGGGCGTAGAGGGTGTGCACCAGAACAATCTGACGCGGGTTTATCAGCGATTTGCCGGCAAAGCCCAACTg    >  2:177661/1‑150 (MQ=255)                                                                                                                                                  aCCGCGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGtt  <  1:656069/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    ACCCGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTT  >  NC_000913/649531‑649826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑