Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,087,671	T→C	H59R (CAC→CGC)	pgaC ←	biofilm PGA synthase PgaCD, catalytic subunit; poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,087,671	0	T	C	100.0%	264.0 / NA	74	H59R (CAC→CGC)	pgaC	biofilm PGA synthase PgaCD, catalytic subunit; poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/37);  total (37/37)

TCAATGTTCTCATAACGCTGTGCTAAAGCGGCGTGTATGGTTTCCTCAACGTTTTTCTCCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGTGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACATAATA  >  NC_000913/1087530‑1087818                                                                                                                                              |                                                                                                                                                    tCAATGTTCTCATAACGCTGTGCTAAAGCGGCGTGTATGGTTTCCTCAACGTTTTTCTCCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCa                                                                                                                                             <  1:508220/150‑1 (MQ=255)  cAATGTTCTCATAACGCTGTGCTAAAGCGGCGTGTATGGTTTCCTCAACGTTTTTCTCCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCAc                                                                                                                                            <  2:408969/150‑1 (MQ=255)      gTTCTCATAACGCTGTGCTAAAGCGGCGTGTATGGTTTCCTCAACGTTTTTCTCCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAg                                                                                                                                       <  1:954013/151‑1 (MQ=255)        tctcATAACGCTGTGCTAAAGCGGCGTGTATGGTTTCCTCAACGTTTTTCTCCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGa                                                                                                                                      <  1:646055/150‑1 (MQ=255)        tctcATAACGCTGTGCTAAAGCGGCGTGTATGGTTTCCTCAACGTTTTTCTCCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGa                                                                                                                                      <  2:744550/150‑1 (MQ=255)         ctcATAACGCTGTGCTAAAGCGGCGTGTATGGTTTCCTCAACGTTTTTCTCCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGAcc                                                                                                                                    >  2:77604/1‑151 (MQ=255)          tcATAACGCTGTGCTAAAGCGGCGTGTATGGTTTCCTCAACGTTTTTCTCCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGAccc                                                                                                                                   >  2:392346/1‑151 (MQ=255)          tcATAACGCTGTGCTAAAGCGGCGTGTATGGTTTCCTCAACGCTTTTCTCCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCACGCGGCGTTCACGATAGAccc                                                                                                                                   >  1:440551/1‑151 (MQ=255)             tAACGCTGTGCTAAAGCGGCGTGTATGGATTTCTCAACGTTTTTCTCCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTTGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGAcc                                                                                                                                    >  1:255572/1‑147 (MQ=255)              aaCGCTGTGCTAAAGCGGCGTGTATGGTTTCCTCAACGTTTTTCTCCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGa                                                                                                                                >  2:102748/1‑150 (MQ=255)                  cTGTGCTAAAGCGGCGTGTATGGTTTCCTCAACGTTTTTCTCCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAAt                                                                                                                             >  1:508150/1‑149 (MQ=255)                    gtgCTAAAGCGGCGTGTATGGTTTCCTCAACGGTTTTCTCCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGAc                                                                                                                         >  2:321799/1‑151 (MQ=255)                     tgCTAAAGCGGCGTGTATGGTTTCCTCAACGTTTTTCTCCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGAc                                                                                                                         >  2:815108/1‑150 (MQ=255)                             cggcgTGTATGGTTTCCTCAACGTTTTTCTCCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCcaac                                                                                                                <  2:713410/151‑1 (MQ=255)                              ggcgTGTATGGTTTCCTCAACGTTTTTCTCCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCcaac                                                                                                                >  1:637880/1‑150 (MQ=255)                               gcgTGTATGGTTTCCTCAACGTTTTTCTCCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCcaacaa                                                                                                              <  2:385842/151‑1 (MQ=255)                                     aTGGTTTCCTCAACGTTTTTCTCCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCcaca                                                                                                        <  2:835646/151‑1 (MQ=255)                                       ggTTTCCTCAACGTTTTTCTCCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATa                                                                                                      <  1:947185/151‑1 (MQ=255)                                            ccTCAACGTTTTTCTCCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATgg                                                                                                  >  1:788320/1‑150 (MQ=255)                                             cTCAACGTTTTTCTCCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGAc                                                                                                >  2:953436/1‑151 (MQ=255)                                               cAACGTTTTTCTCCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGAc                                                                                                <  1:178065/149‑1 (MQ=255)                                               cAACGTTTTTCTCCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACAt                                                                                              >  1:74959/1‑151 (MQ=255)                                                 aCGTTTTTCTCCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCTTTTTCTCCCCACCGCCAGCGGCGTTCACGATAGACCCGGAAATAGACGCCGCCAACAATCCACATAATGGACATaa                                                                                            <  2:549437/151‑1 (MQ=255)                                                 aCGTTTTTCTCCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATaa                                                                                            <  1:245117/151‑1 (MQ=255)                                                    tttttCTCCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATaaaa                                                                                          <  2:641207/150‑1 (MQ=255)                                                     ttttCTCCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATaaaaa                                                                                         >  1:398868/1‑150 (MQ=255)                                                     ttttCTCCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATaaaaa                                                                                         <  2:351274/150‑1 (MQ=255)                                                     ttttCTCCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATaaaaa                                                                                         <  2:128946/150‑1 (MQ=255)                                                     ttttCTCCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATaaaaa                                                                                         >  1:959345/1‑150 (MQ=255)                                                        tctcCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACgg                                                                                     >  2:992850/1‑151 (MQ=255)                                                         ctcctcATTAAAACAGGGGATGATAATGGAGATAGACGGATTATTTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAACTAGACGCCGACAACAATCCACATAATGGACATAAAAAACg                                                                                      >  2:1055645/1‑149 (MQ=255)                                                          tcctcATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGcc                                                                                   >  1:287216/1‑151 (MQ=255)                                                              cATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCagaa                                                                               <  1:122794/151‑1 (MQ=255)                                                               aataagtcAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACGGGGGAGCAGGTGAGGTTTCTCCCCACGGCCACCGACGTTCGCGATAGACCAAGAAATAGACGCCGCAAACAATCCGCATAATGGGCATAAAAAACGGCCagaa                                                                               <  2:684977/143‑1 (MQ=255)                                                               aTTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCagaag                                                                              <  1:215037/151‑1 (MQ=255)                                                               aTTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCaga                                                                                >  2:873327/1‑149 (MQ=255)                                                                 tAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCagaaga                                                                             >  1:854210/1‑150 (MQ=255)                                                                  aaaaCAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAgaagaa                                                                            <  2:970020/150‑1 (MQ=255)                                                                    aaCAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAAc                                                                         >  1:726727/1‑151 (MQ=255)                                                                       aGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGaa                                                                      <  2:290746/151‑1 (MQ=255)                                                                         ggAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAAcc                                                                    >  1:424959/1‑151 (MQ=255)                                                                         ggAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAAcc                                                                    >  2:951616/1‑151 (MQ=255)                                                                             tGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCtcat                                                                >  1:219808/1‑151 (MQ=255)                                                                                tAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCtcatcat                                                             >  1:66721/1‑151 (MQ=255)                                                                                 aaTGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCtcatcatc                                                            <  1:92372/151‑1 (MQ=255)                                                                                 aaTGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCtcatcat                                                             <  1:93262/150‑1 (MQ=255)                                                                                 aaTGGAGATAGACGGATTATCTTTCAACGGGGGAGCTGGTGCGTTTTCTCCCCTCGGCCAGCGGAGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAGCGGCCAGAAGAAAACGAACCtcatctt                                                             <  2:776135/150‑3 (MQ=255)                                                                                  aTGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCtcatcatc                                                            >  2:428108/1‑150 (MQ=255)                                                                                      agaTAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTc                                                       >  1:976852/1‑151 (MQ=255)                                                                                        aTAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCAc                                                     >  1:492553/1‑151 (MQ=255)                                                                                           gACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCag                                                  <  1:874061/151‑1 (MQ=255)                                                                                             cGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCagag                                                <  1:602906/151‑1 (MQ=255)                                                                                                aTTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTg                                              >  1:19072/1‑150 (MQ=255)                                                                                                 ttATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGa                                             >  1:734944/1‑150 (MQ=255)                                                                                                 ttATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGa                                             >  1:819239/1‑150 (MQ=255)                                                                                                   aTCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGaaa                                           <  2:565253/150‑1 (MQ=255)                                                                                                       ttCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGAAAGtacg                                      >  1:1011606/1‑151 (MQ=255)                                                                                                       ttCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGAAAGta                                        <  2:792929/149‑1 (MQ=255)                                                                                                        tCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGAAAGtacgc                                     >  1:187364/1‑151 (MQ=255)                                                                                                         cAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGAAAGtacgc                                     <  1:708205/150‑1 (MQ=255)                                                                                                          aaCTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGAAAGtacgcta                                   <  2:106573/151‑1 (MQ=255)                                                                                                          aaCTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGAAAGtacgct                                    >  2:364478/1‑150 (MQ=255)                                                                                                               gggAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGAAAGTACGCTACGCAt                              >  2:716706/1‑151 (MQ=255)                                                                                                               gggAGCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGAAAGTACGCTACGCAt                              >  2:1018894/1‑151 (MQ=255)                                                                                                                   gCTGGTGCGTTTTCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGAAAGTACGCTACGCATAgg                           <  1:248141/150‑1 (MQ=255)                                                                                                                              ttCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATaa                <  1:1033352/150‑1 (MQ=255)                                                                                                                              ttCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAac               >  2:396391/1‑151 (MQ=255)                                                                                                                              ttCTCCCCACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAac               >  1:159711/1‑151 (MQ=255)                                                                                                                                    ccACGGCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGa         <  2:508867/151‑1 (MQ=255)                                                                                                                                        ggCCAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACata     <  2:398858/151‑1 (MQ=255)                                                                                                                                          ccAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACataat   <  1:859107/151‑1 (MQ=255)                                                                                                                                          ccAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACataat   <  2:739653/151‑1 (MQ=255)                                                                                                                                           cAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACataata  <  2:173035/151‑1 (MQ=255)                                                                                                                                           cAGCGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACataata  <  1:958301/151‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                    TCAATGTTCTCATAACGCTGTGCTAAAGCGGCGTGTATGGTTTCCTCAACGTTTTTCTCCTCATTAAAACAGGGAATGATAATGGAGATAGACGGATTATCTTTCAACTGGGGAGCTGGTGCGTTTTCTCCCCACGGCCAGTGGCGTTCACGATAGACCCAGAAATAGACGCCGCCAACAATCCACATAATGGACATAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACATAATA  >  NC_000913/1087530‑1087818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑