Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,257,682	A→G	V44A (GTA→GCA)	ychF ←	catalase inhibitor protein; ATPase, K+‑dependent, ribosome‑associated

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,257,682	0	A	G	100.0%	192.9 / NA	53	V44A (GTA→GCA)	ychF	catalase inhibitor protein; ATPase, K+‑dependent, ribosome‑associated
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/26);  total (27/26)

TGGTCAGGAACTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTACGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGATACCGCATTTGAATCCCATGATTTAA  >  NC_000913/1257536‑1257819                                                                                                                                                   |                                                                                                                                          tGGTCAGGAACTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTgcgac                                                                                                                                       >  2:146892/1‑151 (MQ=255)   gTCAGGAACTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGc                                                                                                                                     <  1:1016894/151‑1 (MQ=255)     cAGGAACTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCt                                                                                                                                   <  1:107398/151‑1 (MQ=255)     cAGGAACTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCt                                                                                                                                   >  1:423877/1‑151 (MQ=255)         aaCTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTgtgt                                                                                                                               <  1:559120/151‑1 (MQ=255)         aaCTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTgtgt                                                                                                                               <  1:221989/151‑1 (MQ=255)          aCTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGtt                                                                                                                              >  1:598313/1‑151 (MQ=255)              gTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGc                                                                                                                          >  2:989146/1‑151 (MQ=255)                tACCCAGACCTTCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCt                                                                                                                         <  2:566397/150‑1 (MQ=255)                   ccAGACCTTCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAAt                                                                                                                     <  1:192743/151‑1 (MQ=255)                    cAGACCTTCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATg                                                                                                                    >  2:469327/1‑151 (MQ=255)                                tttCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATgg                                                                                                         >  2:4169/1‑150 (MQ=255)                                 ttCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATgg                                                                                                         <  1:496630/149‑1 (MQ=255)                                   cGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGaa                                                                                                       >  2:741871/1‑149 (MQ=255)                                    gATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGt                                                                                                    >  2:346970/1‑151 (MQ=255)                                         gcCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGcc                                                                                               <  2:480774/151‑1 (MQ=255)                                           cTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGc                                                                                             >  1:886457/1‑151 (MQ=255)                                             tttACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTACGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCt                                                                                            <  2:1002287/150‑1 (MQ=255)                                              ttACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTc                                                                                          >  1:384617/1‑151 (MQ=255)                                                 ccAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAAt                                                                                       <  1:560852/151‑1 (MQ=255)                                                   aGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATAc                                                                                     <  2:408051/151‑1 (MQ=255)                                                      ccGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCgg                                                                                  >  2:676295/1‑151 (MQ=255)                                                       cGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGc                                                                                 >  2:494161/1‑151 (MQ=255)                                                        ggCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCt                                                                                >  2:705291/1‑151 (MQ=255)                                                           gATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTg                                                                             >  1:667275/1‑151 (MQ=255)                                                              atCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTc                                                                          >  1:277864/1‑151 (MQ=255)                                                                  aCAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAgcg                                                                      <  1:123363/151‑1 (MQ=255)                                                                    aaaTTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGcgcg                                                                    >  2:483164/1‑151 (MQ=255)                                                                    aaaTTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGcgcg                                                                    >  1:474621/1‑151 (MQ=255)                                                                    aaaTTACATGGCCGTGGGAAGCGTACCCTGCGGTTTTACGATTACAGCCAGTTGATCCAGTCGAGTCTCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCATAATGGATAGTTGGCCTCTTCAATAACGGCTTTGGTCAGcgcg                                                                    >  2:1034536/1‑151 (MQ=255)                                                                         ccATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGa                                                                >  1:229626/1‑150 (MQ=255)                                                                          cATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAAc                                                              >  1:237282/1‑151 (MQ=255)                                                                           aTGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACa                                                             <  1:41039/151‑1 (MQ=255)                                                                           aTGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACa                                                             <  2:525078/151‑1 (MQ=255)                                                                             ggTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAg                                                            <  1:493707/150‑1 (MQ=255)                                                                                cGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGt                                                         <  1:318389/150‑1 (MQ=255)                                                                                   gggAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGAt                                                     <  2:955975/151‑1 (MQ=255)                                                                                        gCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTccc                                                <  1:209864/151‑1 (MQ=255)                                                                                                 gCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTggg                                       >  1:454511/1‑151 (MQ=255)                                                                                                   ggTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGc                                      >  2:387528/1‑150 (MQ=255)                                                                                                         aCGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACcga                               <  2:583752/151‑1 (MQ=255)                                                                                                            aTTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACcgacg                             >  2:163316/1‑150 (MQ=255)                                                                                                              ttCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGAt                           >  2:914619/1‑150 (MQ=255)                                                                                                                 aGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGATAcc                        <  2:103142/150‑1 (MQ=255)                                                                                                                  gCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGATACCGc                      <  2:39819/151‑1 (MQ=255)                                                                                                                   ccAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGATACCGc                      >  2:814405/1‑150 (MQ=255)                                                                                                                        tGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGATACCGCATTTg                 >  1:494770/1‑150 (MQ=255)                                                                                                                        tGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGATACCGCATTTg                 <  1:205389/150‑1 (MQ=255)                                                                                                                         gATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGATACCGCATTTGa                <  1:494175/150‑1 (MQ=255)                                                                                                                          aTCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGATACCGCATTTGAAt              <  1:693698/151‑1 (MQ=255)                                                                                                                              aGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGATACCGCATTTGAATccc           >  2:316650/1‑150 (MQ=255)                                                                                                                               ggCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGATACCGCATTTGAATCCCAt         <  1:550297/151‑1 (MQ=255)                                                                                                                                   aGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGATACCGCATTTGAATCCCATGAtt     <  1:237679/151‑1 (MQ=255)                                                                                                                                       tCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGATACCGCATTTGAATCCCATGATTTaa  >  1:195395/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                          TGGTCAGGAACTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTACGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGATACCGCATTTGAATCCCATGATTTAA  >  NC_000913/1257536‑1257819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑