Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,356,140 T→C N324S (AAC→AGC)  sapA ← antimicrobial peptide transport ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,356,1400TC100.0% 215.2 / NA 59N324S (AAC→AGC) sapAantimicrobial peptide transport ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/24);  total (35/24)

CCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATA  >  NC_000913/1355996‑1356278
                                                                                                                                                |                                                                                                                                          
cccAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTaa                                                                                                                                      <  1:607502/151‑1 (MQ=255)
    aCGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGc                                                                                                                                  <  1:733/151‑1 (MQ=255)
     cGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGcc                                                                                                                                 >  2:1312/1‑151 (MQ=255)
      gACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGcc                                                                                                                                 >  1:534286/1‑150 (MQ=255)
      gACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGcc                                                                                                                                 >  1:385042/1‑150 (MQ=255)
           cAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGcc                                                                                                                           >  2:584520/1‑151 (MQ=255)
           cAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGcc                                                                                                                           >  2:39965/1‑151 (MQ=255)
            aaCTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGcc                                                                                                                           >  2:884366/1‑150 (MQ=255)
              cTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAgc                                                                                                                        >  2:184117/1‑151 (MQ=255)
                gTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAgcgc                                                                                                                      >  1:697163/1‑151 (MQ=255)
                    gcgcgATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATgg                                                                                                                  >  1:292307/1‑151 (MQ=255)
                         aTTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGctatt                                                                                                                                        >  2:90412/1‑124 (MQ=255)
                         aTTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGctatt                                                                                                                                        <  1:90414/124‑1 (MQ=255)
                         aTTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGAc                                                                                                             >  2:293014/1‑151 (MQ=255)
                          tttCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGAc                                                                                                             >  2:1052915/1‑150 (MQ=255)
                            tCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTTATCGCCAGTGCCAGCGCATGGCGGACAGc                                                                                                          >  1:180749/1‑151 (MQ=255)
                            tCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGc                                                                                                          >  2:74812/1‑151 (MQ=255)
                             cGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGc                                                                                                          <  2:242913/150‑1 (MQ=255)
                             cGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCg                                                                                                         >  2:1042876/1‑151 (MQ=255)
                               ccGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCggg                                                                                                       <  1:379415/151‑1 (MQ=255)
                               ccGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCggg                                                                                                       >  1:279977/1‑151 (MQ=255)
                               ccGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCggg                                                                                                       >  2:347011/1‑151 (MQ=255)
                               ccGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCggg                                                                                                       >  2:117992/1‑151 (MQ=255)
                                cGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCggg                                                                                                       >  2:498189/1‑150 (MQ=255)
                                 ggATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGat                                                                                                     >  2:210570/1‑151 (MQ=255)
                                  gATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGatt                                                                                                    >  1:1011968/1‑151 (MQ=255)
                                  gATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGatt                                                                                                    >  2:287751/1‑151 (MQ=255)
                                      gTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGattatt                                                                                                 <  1:122513/150‑1 (MQ=255)
                                      gTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTAttt                                                                                                <  2:581090/151‑1 (MQ=255)
                                        aTTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTa                                                                                               <  1:1053017/150‑1 (MQ=255)
                                           cAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAgcg                                                                                            <  2:424557/150‑1 (MQ=255)
                                             gTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAgcggcg                                                                                         >  1:133370/1‑151 (MQ=255)
                                             gTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAgcggcg                                                                                         >  2:400744/1‑151 (MQ=255)
                                               aaTTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGt                                                                                       >  1:114471/1‑151 (MQ=255)
                                                   ttAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGtttt                                                                                    >  2:879276/1‑149 (MQ=255)
                                                    tAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGttt                                                                                     >  2:491385/1‑148 (MQ=255)
                                                      gCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCgg                                                                                <  1:668783/151‑1 (MQ=255)
                                                       ccTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGgt                                                                               <  1:914844/151‑1 (MQ=255)
                                                       ccTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTCTTGCggg                                                                               >  2:358777/1‑150 (MQ=255)
                                                          cGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGttttggggggt                                                                             >  1:956443/1‑142 (MQ=255)
                                                          cGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGtt                                                                            <  1:292894/151‑1 (MQ=255)
                                                           gTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTa                                                                           <  1:925671/151‑1 (MQ=255)
                                                             tGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGGGgtggtta                                                                          >  1:681895/1‑146 (MQ=255)
                                                                       ccACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAg                                                               <  1:448513/151‑1 (MQ=255)
                                                                          cGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGGTTGgggggggtta                                                                          >  2:1030686/1‑131 (MQ=255)
                                                                             ggCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACg                                                         <  2:462976/151‑1 (MQ=255)
                                                                                gcgcgGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTc                                                      <  2:1039682/151‑1 (MQ=255)
                                                                                     gTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATccc                                                 <  2:516607/151‑1 (MQ=255)
                                                                                     gTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATccc                                                 <  1:928244/151‑1 (MQ=255)
                                                                                       aaaaTAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCa                                                <  2:626622/150‑1 (MQ=255)
                                                                                        aaaTAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAg                                               <  1:685724/150‑1 (MQ=255)
                                                                                                 ccgccgTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGGGTTAAATGCCCGATAGGCGGCGTTCATCCCAGGACGCa                                         >  1:9226/1‑147 (MQ=255)
                                                                                                          cAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCa                             <  1:210397/150‑1 (MQ=255)
                                                                                                            gCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAgc                          <  1:643216/151‑1 (MQ=255)
                                                                                                                   cATAATAGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGgtc                   <  2:135417/151‑1 (MQ=255)
                                                                                                                    ataataGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGGGTTTAAAGCCAGAAAGGGGACGTTCCTCCCCAGACGCCGCGTTTAACGCAAACGCggg                     >  1:991381/1‑148 (MQ=255)
                                                                                                                     taataGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTTGGGGGGTTAAATGCCACAAAGGCGACGTTCATCCCCGGACGCAGCGTTTAACGCAAGCGCGGggcg                  >  2:799883/1‑150 (MQ=255)
                                                                                                                     taataGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGGGGGGGTAAAAGCCAGAAAGGGGACGGTCATTCCAGGGCGCAGGGTTAAAAGCAAGCGGGGggcgg                 >  2:514327/1‑150 (MQ=255)
                                                                                                                      aataGATGGATTGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGgtcgt                 <  2:269350/150‑1 (MQ=255)
                                                                                                                                ttGCATCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGGGGGGGTTAAAACCAGATAGGGGGCGGTCATCCCCAGAAGCAACGGTAAAAGCAAACGCGGGGCGGCACGCaaaa        >  2:442296/1‑149 (MQ=255)
                                                                                                                                    aTCAGGCGCTGGCTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATa  <  1:1032633/151‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                          
CCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATA  >  NC_000913/1355996‑1356278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: