Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	124,759	A→G	A581A (GCA→GCG)	aceE →	pyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	124,759	0	A	G	100.0%	236.3 / NA	65	A581A (GCA→GCG)	aceE	pyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/31);  total (34/31)

GTTCCGTCAGATTGGTATTTACAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAGCGCGTGGCTTCCTGATCGGCGGTACTTCCGG  >  NC_000913/124612‑124905                                                                                                                                                    |                                                                                                                                                   gTTCCGTCAGATTGGTATTTACAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTggcggc                                                                                                                                                  <  1:1032699/150‑1 (MQ=255) gTTCCGTCAGATTGGTATTTACAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGgcggcg                                                                                                                                                 <  2:341493/151‑1 (MQ=255)  ttCCGTCAGATTGGTATTTACAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGcggcgg                                                                                                                                                >  1:1022236/1‑151 (MQ=255)   tCCGTCAGATTGGTATTTACAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTggcggcggc                                                                                                                                               >  2:784583/1‑151 (MQ=255)       tCAGATTGGTATTTACAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGAcc                                                                                                                                           >  2:400347/1‑151 (MQ=255)        cAGATTGGTATTTACAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACct                                                                                                                                          <  1:855033/151‑1 (MQ=255)          gATTGGTATTTACAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACctct                                                                                                                                        >  2:375080/1‑151 (MQ=255)          gATTGGTATTTACAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACctct                                                                                                                                        >  2:682727/1‑151 (MQ=255)           aTTGGTATTTACAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCtt                                                                                                                                       <  1:916895/151‑1 (MQ=255)             tGGTATTTACAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTa                                                                                                                                      <  1:762893/150‑1 (MQ=255)               gTATTTACAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAg                                                                                                                                   <  1:153880/151‑1 (MQ=255)                 aTTTACAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGc                                                                                                                                  <  1:251547/150‑1 (MQ=255)                         cccGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAAt                                                                                                                         <  2:141757/151‑1 (MQ=255)                         cccGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAAt                                                                                                                         <  1:296889/151‑1 (MQ=255)                           cGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCt                                                                                                                       <  1:377886/151‑1 (MQ=255)                           cGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCt                                                                                                                       >  1:1055422/1‑151 (MQ=255)                             aaCGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGc                                                                                                                     <  2:14077/151‑1 (MQ=255)                             aaCGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGc                                                                                                                     >  1:89236/1‑151 (MQ=255)                                   cagcagTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCgatga                                                                                                               <  2:234078/151‑1 (MQ=255)                                   cagcagTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCgatg                                                                                                                <  2:468302/150‑1 (MQ=255)                                    agcagTACACCCCGCAGGACCGCGAGCCGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCgatgat                                                                                                              >  1:160497/1‑151 (MQ=255)                                       agTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATccc                                                                                                           <  1:287450/151‑1 (MQ=255)                                       agTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATccc                                                                                                           >  1:1058069/1‑151 (MQ=255)                                          acacCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGt                                                                                                         <  1:809495/150‑1 (MQ=255)                                          acacCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGt                                                                                                         <  2:740018/150‑1 (MQ=255)                                           cacCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTc                                                                                                       >  1:325248/1‑151 (MQ=255)                                            acCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCt                                                                                                      <  2:759179/151‑1 (MQ=255)                                               ccGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACa                                                                                                   <  1:806195/151‑1 (MQ=255)                                                        cgcgAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGAGCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCGCCAACAATCTGCCGATGATCCCGTTCTACATCTATTACt                                                                                          <  2:826563/151‑1 (MQ=255)                                                         gcgAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTc                                                                                         <  1:693402/151‑1 (MQ=255)                                                          cgAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCg                                                                                        >  2:1007299/1‑151 (MQ=255)                                                           gAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCg                                                                                        >  1:651136/1‑150 (MQ=255)                                                             gCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATg                                                                                     >  1:325320/1‑151 (MQ=255)                                                             gCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATg                                                                                     <  2:741593/151‑1 (MQ=255)                                                              catgTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGt                                                                                    >  2:238203/4‑151 (MQ=255)                                                                 gtttgtttcTATAAAGGAGGCGAGAAAGGTCAGATTCTGCAGGAAGGGATCAAAGAGCTTGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCg                                                                                 <  1:987270/143‑1 (MQ=255)                                                                      ttACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTc                                                                            >  1:158159/1‑151 (MQ=255)                                                                       tACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTcc                                                                           >  2:783309/1‑151 (MQ=255)                                                                           ataAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCg                                                                       >  2:1008120/1‑151 (MQ=255)                                                                              aagaagACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTAt                                                                    >  2:52793/1‑151 (MQ=255)                                                                              aagaagACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTAt                                                                    >  2:718225/1‑151 (MQ=255)                                                                                 aagaCGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTg                                                                  >  1:58995/1‑150 (MQ=255)                                                                                    aCGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCg                                                               >  2:14332/1‑150 (MQ=255)                                                                                       agaAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCt                                                           <  1:311719/151‑1 (MQ=255)                                                                                       agaAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCt                                                           >  1:1064473/1‑151 (MQ=255)                                                                                        gaAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCtg                                                          >  2:371216/1‑151 (MQ=255)                                                                                            ggTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCt                                                      <  2:593751/151‑1 (MQ=255)                                                                                            ggTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCt                                                      <  1:803942/151‑1 (MQ=255)                                                                                               cAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTgg                                                    <  1:763284/150‑1 (MQ=255)                                                                                                     cTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCt                                              >  1:1044843/1‑150 (MQ=255)                                                                                                     cTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTg                                             >  2:193763/1‑151 (MQ=255)                                                                                                     cTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTg                                             >  2:224317/1‑151 (MQ=255)                                                                                                       gCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTg                                             <  2:301891/149‑1 (MQ=255)                                                                                                        cAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCg                                          >  2:879345/1‑151 (MQ=255)                                                                                                           gAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGAcc                                       >  1:249101/1‑151 (MQ=255)                                                                                                                  tCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAgc                                >  1:987022/1‑151 (MQ=255)                                                                                                                        aGCTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAGCGCGTgg                          <  1:717907/151‑1 (MQ=255)                                                                                                                          cTGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAGCGCGTg                           >  2:676591/1‑148 (MQ=255)                                                                                                                           tGGGCGCAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAGCGCGTGGCtt                       >  1:149882/1‑151 (MQ=255)                                                                                                                                gcAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCCAGCGCGTGGCTTCCTg                   >  2:557892/1‑150 (MQ=255)                                                                                                                                gcAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAGCGCGTGGCTTCCTGa                  <  2:551616/151‑1 (MQ=255)                                                                                                                                 cAGGTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAGCGCGTGGCTTCCTGAt                 <  2:27754/151‑1 (MQ=255)                                                                                                                                   ggTTGTTCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAGCGCGTGGCTTCCTGATcg               >  1:1056777/1‑151 (MQ=255)                                                                                                                                    gttgttCCTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAGCGCGTGGCTTCCTGATcgg              <  1:769685/151‑1 (MQ=255)                                                                                                                                          ccTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAGCGCGTGGCTTCCTGATCGGCGGTa         <  1:20738/150‑1 (MQ=255)                                                                                                                                          ccTGGCTGGCGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAGCGCGTGGCTTCCTGATCGGCGGTAc        <  1:879378/151‑1 (MQ=255)                                                                                                                                              gctggcGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAGCGCGTGGCTTCCTGATCGGCGGTACTTcc    >  1:379383/1‑151 (MQ=255)                                                                                                                                                tggcGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAGCGCGTGGCTTCCTGATCGGCGGTACTTCCgg  >  2:895311/1‑151 (MQ=255)                                                                                                                                                tggcGGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAGCGCGTGGCTTCCTGATCGGCGGTACTTCCgg  >  1:911380/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GTTCCGTCAGATTGGTATTTACAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAGCGCGTGGCTTCCTGATCGGCGGTACTTCCGG  >  NC_000913/124612‑124905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑