Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,628,190	A→G	H225R (CAC→CGC)	ydfG →	NADP‑dependent 3‑hydroxy acid dehydrogenase; malonic semialdehyde reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,628,190	0	A	G	100.0%	261.9 / NA	73	H225R (CAC→CGC)	ydfG	NADP‑dependent 3‑hydroxy acid dehydrogenase; malonic semialdehyde reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/40);  total (33/40)

GAACCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCA  >  NC_000913/1628042‑1628334                                                                                                                                                     |                                                                                                                                                 gAACCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTcgcg                                                                                                                                                >  2:378877/1‑151 (MQ=255)  aaCCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGt                                                                                                                                               >  2:665628/1‑151 (MQ=255)  aaCCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGt                                                                                                                                               >  1:410553/1‑151 (MQ=255)      gggTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGtcaat                                                                                                                                           <  1:256450/151‑1 (MQ=255)       ggTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATTCCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGtcaat                                                                                                                                           <  2:935698/150‑1 (MQ=255)             gtgggtggTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGtcaatatc                                                                                                                                        <  2:838415/147‑1 (MQ=255)                 gtggtACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCt                                                                                                                                <  2:633814/151‑1 (MQ=255)                 gtggtACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCt                                                                                                                                <  2:485608/151‑1 (MQ=255)                           gTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAatgatg                                                                                                                      >  1:596353/1‑151 (MQ=255)                            ttttCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGc                                                                                                                     >  1:51060/1‑151 (MQ=255)                              ttCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCg                                                                                                                   >  1:827588/1‑151 (MQ=255)                              ttCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCg                                                                                                                   >  1:49656/1‑151 (MQ=255)                                ccAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCgg                                                                                                                  <  1:588267/150‑1 (MQ=255)                                 cAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGtt                                                                                                                <  1:788144/151‑1 (MQ=255)                                 cAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGt                                                                                                                 <  1:601141/150‑1 (MQ=255)                                     gTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTAcc                                                                                                             <  2:599019/150‑1 (MQ=255)                                        cGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCaaa                                                                                                         <  1:487007/151‑1 (MQ=255)                                        cGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCaaa                                                                                                         <  2:505237/151‑1 (MQ=255)                                          cTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGc                                                                                                       >  2:778277/1‑151 (MQ=255)                                            ttAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCt                                                                                                      >  1:38517/1‑150 (MQ=255)                                               aaGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGc                                                                                                  >  2:136788/1‑151 (MQ=255)                                                  gCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCgg                                                                                               <  1:645945/151‑1 (MQ=255)                                                    gATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGAc                                                                                             >  2:938432/1‑151 (MQ=255)                                                    gATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGAc                                                                                             >  2:787601/1‑151 (MQ=255)                                                        aCGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGa                                                                                          >  1:911218/1‑150 (MQ=255)                                                         cGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAAt                                                                                        <  2:391942/151‑1 (MQ=255)                                                              aaGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCa                                                                                   <  2:629028/151‑1 (MQ=255)                                                                 cAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCg                                                                                >  2:93093/1‑151 (MQ=255)                                                                  aGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGt                                                                               >  1:388920/1‑151 (MQ=255)                                                                    aaaaaaCCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCa                                                                             <  1:285295/151‑1 (MQ=255)                                                                     aaaaaCCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTc                                                                              >  2:731456/1‑149 (MQ=255)                                                                       aaaCCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGt                                                                           >  1:397416/1‑150 (MQ=255)                                                                       aaaCCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTa                                                                          <  2:24287/151‑1 (MQ=255)                                                                         aCCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAAt                                                                        >  1:68190/1‑151 (MQ=255)                                                                         aCCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAAt                                                                        >  1:653451/1‑151 (MQ=255)                                                                           cTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAAttt                                                                      >  1:120821/1‑151 (MQ=255)                                                                           cTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAAttt                                                                      >  1:998647/1‑151 (MQ=255)                                                                            tATCAAAATACTGCTGCATTGGCGCGAGAAGATGTCAGCGATGCCGTCTGGTGGCTGTCACCGCCGCCTGCTCGTGTCAATAACAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCTGGACTGAATGTCCACCGCCGGTAAttt                                                                      >  2:311087/1‑150 (MQ=255)                                                                            tATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGGCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACTGTCAGTAAtttt                                                                     >  1:364191/1‑151 (MQ=255)                                                                             aTCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAAttttt                                                                    <  2:1047965/151‑1 (MQ=255)                                                                              tCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAAttttt                                                                    >  1:77108/1‑150 (MQ=255)                                                                                aaaaTACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTtata                                                                 <  2:748917/151‑1 (MQ=255)                                                                                 aaaTACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATAc                                                                >  1:820331/1‑151 (MQ=255)                                                                                          tGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTa                                                        >  1:775110/1‑150 (MQ=255)                                                                                            cATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACt                                                     <  2:45655/151‑1 (MQ=255)                                                                                            cATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACt                                                     <  2:271829/151‑1 (MQ=255)                                                                                             aTTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTg                                                    <  2:118172/151‑1 (MQ=255)                                                                                               tGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGcc                                                  >  2:372340/1‑151 (MQ=255)                                                                                               tGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGc                                                   <  2:660069/150‑1 (MQ=255)                                                                                                gACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCg                                                 >  1:353886/1‑151 (MQ=255)                                                                                                gACACCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGcc                                                  <  1:1033448/150‑1 (MQ=255)                                                                                                   gCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGt                                              <  1:745478/151‑1 (MQ=255)                                                                                                           aTGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCtt                                      >  1:962088/1‑151 (MQ=255)                                                                                                             gTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGt                                    <  2:457875/151‑1 (MQ=255)                                                                                                                aGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTcac                                 >  1:311605/1‑151 (MQ=255)                                                                                                                   gAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACaaa                              <  2:604634/151‑1 (MQ=255)                                                                                                                   gAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACaaa                              <  1:1052066/151‑1 (MQ=255)                                                                                                                   gAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACaaa                              <  2:331174/151‑1 (MQ=255)                                                                                                                    aaGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACaaaa                             <  2:984034/151‑1 (MQ=255)                                                                                                                     aGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACaaaa                             >  2:439854/1‑150 (MQ=255)                                                                                                                      gCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACaaaaaa                           <  2:318751/151‑1 (MQ=255)                                                                                                                      gCCGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACaaaaaa                           <  1:914535/151‑1 (MQ=255)                                                                                                                       ccGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACaaaaa                            >  1:206763/1‑149 (MQ=255)                                                                                                                        cGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGt                         >  1:757232/1‑151 (MQ=255)                                                                                                                        cGTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGt                         <  2:1052512/151‑1 (MQ=255)                                                                                                                         gTCTGGTGGGTGTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTg                        <  1:757006/151‑1 (MQ=255)                                                                                                                             ggtgggtgTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAg                    <  2:164305/151‑1 (MQ=255)                                                                                                                                gggtgTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCTGACTGAATGTCCATCGTCAGTAATTTTTATACCCGGCGTAACTGACGGGTTATTGCTTGTCACAAAAAAGTGGTAGACt                 <  2:285243/151‑1 (MQ=255)                                                                                                                                 ggtgTCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTc                <  1:545401/151‑1 (MQ=255)                                                                                                                                    gtCAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATg             >  1:27941/1‑151 (MQ=255)                                                                                                                                      cAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGc            <  1:507855/150‑1 (MQ=255)                                                                                                                                      cAACGCTGCCTGCTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGc            <  2:426007/150‑1 (MQ=255)                                                                                                                                             gcctgcTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAact    <  1:248651/151‑1 (MQ=255)                                                                                                                                                 gcTCGCGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAactca  <  1:530797/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                 GAACCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCA  >  NC_000913/1628042‑1628334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑