Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,811,829	T→C	V299A (GTG→GCG)	ydjN →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,811,829	0	T	C	98.6%	247.3 / ‑6.6	73	V299A (GTG→GCG)	ydjN	putative transporter
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (32/39);  total (32/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

GCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGG  >  NC_000913/1811681‑1811976                                                                                                                                                     |                                                                                                                                                    gCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTgcgc                                                                                                                                                   <  2:508837/151‑1 (MQ=255)  cAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCt                                                                                                                                                  <  2:593237/151‑1 (MQ=255)     gTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGAc                                                                                                                                               <  1:81051/151‑1 (MQ=255)      ttCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACg                                                                                                                                              >  1:150956/1‑151 (MQ=255)        cTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGtt                                                                                                                                            >  2:797044/1‑151 (MQ=255)           aCTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGc                                                                                                                                         <  2:545023/151‑1 (MQ=255)             ttGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCt                                                                                                                                       >  1:400024/1‑151 (MQ=255)               gCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCtt                                                                                                                                      <  1:94686/150‑1 (MQ=255)                 aaGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTAc                                                                                                                                   >  1:169411/1‑151 (MQ=255)                 aaGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTAc                                                                                                                                   <  2:438643/151‑1 (MQ=255)                   gACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCa                                                                                                                                 <  2:102905/151‑1 (MQ=255)                   gACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCa                                                                                                                                 <  1:377947/151‑1 (MQ=255)                      atcatcAAACTGGGAAGTTTCTTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGcc                                                                                                                              <  2:580232/151‑1 (MQ=255)                             aaCTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTcc                                                                                                                         >  2:147365/1‑149 (MQ=255)                             aaCTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTcc                                                                                                                         >  1:37013/1‑149 (MQ=255)                                 gggAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGtgct                                                                                                                   <  2:400014/151‑1 (MQ=255)                                   gAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGtgctgc                                                                                                                 <  1:159303/151‑1 (MQ=255)                                   gAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTAGGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGtgctgc                                                                                                                 <  2:864945/151‑1 (MQ=255)                                      gTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTc                                                                                                              <  1:735475/151‑1 (MQ=255)                                         tCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGTATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTAt                                                                                                           >  2:696800/1‑151 (MQ=255)                                           gTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATcc                                                                                                         >  2:839577/1‑151 (MQ=255)                                                cgcgTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTg                                                                                                    >  1:291188/1‑151 (MQ=255)                                                 gcgTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTg                                                                                                    >  1:590375/1‑150 (MQ=255)                                                         ccTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCCCTGAAtgt                                                                                               >  2:16097/1‑147 (MQ=255)                                                         ccTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGa                                                                                            <  2:167181/150‑1 (MQ=255)                                                            cGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGc                                                                                         <  1:309482/150‑1 (MQ=255)                                                               tCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGcaca                                                                                      >  2:622148/1‑150 (MQ=255)                                                                ctgctgATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACaa                                                                                     >  1:519062/1‑150 (MQ=255)                                                                  gctgATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAAcg                                                                                  >  2:466513/1‑151 (MQ=255)                                                                   ctgATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAAcgc                                                                                 >  2:909230/1‑151 (MQ=255)                                                                     gATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGcgt                                                                               <  1:12015/151‑1 (MQ=255)                                                                         aTGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCgtcgtc                                                                           >  2:605036/1‑151 (MQ=255)                                                                          tGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGGAGTACTTCCGTAAGGTATGGCCTGCGTTGAGGTTTGCCTTTGCCAGCCGTTCCAGTGCTGCGGCTATCCCACTGAATGTGGAAGCACAAACGCgtcgtc                                                                           <  2:459704/150‑1 (MQ=255)                                                                            tttGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTgg                                                                        >  2:539417/1‑151 (MQ=255)                                                                            tttGCAGTGCATGGCATTCTGCAGGGCATTAATGGCGTGAGTACGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTg                                                                         <  1:338618/150‑1 (MQ=255)                                                                               gCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGc                                                                      <  1:747006/150‑1 (MQ=255)                                                                                 aGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGtt                                                                   <  2:615332/151‑1 (MQ=255)                                                                                  gTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTCCTTCCGTAAGGTATGGCCTGCGCTGCCGTTTGCTTTTCCCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGt                                                                    <  2:279355/149‑1 (MQ=255)                                                                                    gCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCt                                                                >  2:419746/1‑151 (MQ=255)                                                                                     cATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTg                                                               <  2:452690/151‑1 (MQ=255)                                                                                     cATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTg                                                               >  2:497814/1‑151 (MQ=255)                                                                                      aTGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTg                                                               >  2:575594/1‑150 (MQ=255)                                                                                      aTGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGa                                                              <  2:852674/151‑1 (MQ=255)                                                                                        ggCATTCTGCTGGGCATTAATGGCTTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCTCTGACGTTTTCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAAt                                                            <  2:544085/151‑1 (MQ=255)                                                                                        ggCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGaa                                                             <  2:680618/150‑1 (MQ=255)                                                                                         gCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATc                                                           <  1:1031596/151‑1 (MQ=255)                                                                                            ttCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCAt                                                        >  1:542590/1‑151 (MQ=255)                                                                                                gctgGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGc                                                     <  2:954509/150‑1 (MQ=255)                                                                                                gctgGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGc                                                     <  2:453366/150‑1 (MQ=255)                                                                                                    ggCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGtt                                                <  1:997370/151‑1 (MQ=255)                                                                                                       aTTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTcg                                             >  1:829759/1‑151 (MQ=255)                                                                                                            tGGCGTGGCTCCTCTTAAATCCTTCCGTAGGGTATGGCCTGACCCTACGTTTTCCTTCACCAACCGTTCCCGTGCTGCGTCTATCCCACTTAATGTGGAAGCACAAACGCGTCGTCTTGGCTTTCCTGAATCCATCGCCATTTTcgc                                            <  2:646847/147‑1 (MQ=255)                                                                                                             ggCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCct                                       <  1:426808/151‑1 (MQ=255)                                                                                                               cGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCctct                                     >  2:705543/1‑151 (MQ=255)                                                                                                                gTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTCCCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCtt                                    <  2:577888/151‑1 (MQ=255)                                                                                                                gTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCctct                                     <  1:58581/150‑1 (MQ=255)                                                                                                                  gAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTc                                  >  2:560931/1‑151 (MQ=255)                                                                                                                      ccGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTg                              <  2:788815/151‑1 (MQ=255)                                                                                                                       cGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTg                              >  2:88075/1‑150 (MQ=255)                                                                                                                        gCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCa                            <  1:1029375/151‑1 (MQ=255)                                                                                                                               tACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTg                     >  1:231214/1‑151 (MQ=255)                                                                                                                                 cTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTg                     <  1:627577/149‑1 (MQ=255)                                                                                                                                    ccGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGt                   <  1:473563/148‑1 (MQ=255)                                                                                                                                       tAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGaa              <  2:1045523/150‑1 (MQ=255)                                                                                                                                       tAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAAc             >  1:1047098/1‑151 (MQ=255)                                                                                                                                        aaGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGCGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCCTTGGTCAGAAc             >  2:941594/1‑150 (MQ=255)                                                                                                                                         aGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACgg           >  2:625928/1‑151 (MQ=255)                                                                                                                                          ggTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACgg           <  2:521210/150‑1 (MQ=255)                                                                                                                                          ggTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGc          >  1:753215/1‑151 (MQ=255)                                                                                                                                          ggTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGc          <  2:578711/151‑1 (MQ=255)                                                                                                                                           gTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCt         >  2:522974/1‑151 (MQ=255)                                                                                                                                              tGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTgc       <  1:215271/150‑1 (MQ=255)                                                                                                                                              tGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTgc       <  1:116026/150‑1 (MQ=255)                                                                                                                                                  cTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCgg  >  1:217544/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGG  >  NC_000913/1811681‑1811976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑