Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,938,582	A→G	T312A (ACT→GCT)	pykA →	pyruvate kinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,938,582	0	A	G	100.0%	296.6 / NA	77	T312A (ACT→GCT)	pykA	pyruvate kinase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/44);  total (33/44)

TCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTACTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATG  >  NC_000913/1938436‑1938729                                                                                                                                                   |                                                                                                                                                    tCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTg                                                                                                                                                  >  1:1057601/1‑150 (MQ=255)   ggCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACg                                                                                                                                               <  2:754802/151‑1 (MQ=255)    gCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGc                                                                                                                                              >  1:301731/1‑151 (MQ=255)     cATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCt                                                                                                                                             >  1:578479/1‑151 (MQ=255)             aaGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCGGTGATGCt                                                                                                                                     >  2:64204/1‑151 (MQ=255)                cGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTc                                                                                                                                  >  2:217363/1‑151 (MQ=255)                     aTCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGc                                                                                                                               >  1:331972/1‑149 (MQ=255)                        cgtcgtGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGaaa                                                                                                                          <  1:205944/151‑1 (MQ=255)                         gtcgtGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAAc                                                                                                                         <  2:159873/151‑1 (MQ=255)                                 cgTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTg                                                                                                                 <  2:1060929/151‑1 (MQ=255)                                 cgTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTg                                                                                                                 >  2:607498/1‑151 (MQ=255)                                      gCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCa                                                                                                             <  1:33515/150‑1 (MQ=255)                                          aaCCGAGCGGTACTCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATc                                                                                                        >  1:20157/1‑151 (MQ=255)                                          aaCCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATc                                                                                                        <  2:167334/151‑1 (MQ=255)                                            ccGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCg                                                                                                      >  1:382161/1‑151 (MQ=255)                                                gCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTc                                                                                                    >  1:584901/1‑149 (MQ=255)                                                    tAATCACGGCGACCCAGATGATGGAGTCAATGCTTACTAACCCGACCCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGCATGGTGCTGACGCTGCGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAAc                                                                                              >  2:813389/1‑151 (MQ=255)                                                      aTCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCg                                                                                            <  1:228504/151‑1 (MQ=255)                                                        cACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGtt                                                                                          <  2:518273/151‑1 (MQ=255)                                                            gCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGc                                                                                        >  1:749346/1‑149 (MQ=255)                                                              gACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGcc                                                                                    >  2:1045931/1‑151 (MQ=255)                                                               aCCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGc                                                                                     >  2:246361/1‑149 (MQ=255)                                                                cccAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCa                                                                                   <  2:852902/150‑1 (MQ=255)                                                                cccAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCAt                                                                                  <  1:694774/151‑1 (MQ=255)                                                                 ccAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATg                                                                                 <  1:217371/151‑1 (MQ=255)                                                                  cAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGaa                                                                                                <  2:765876/135‑1 (MQ=255)                                                                  cAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGaa                                                                                                >  1:765901/1‑135 (MQ=255)                                                                  cAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATgg                                                                                <  2:158794/151‑1 (MQ=255)                                                                  cAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATgg                                                                                >  1:323630/1‑151 (MQ=255)                                                                    gatgatGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGgc                                                                               >  1:346932/1‑150 (MQ=255)                                                                          ggAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGtt                                                                        <  2:214259/151‑1 (MQ=255)                                                                          ggAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGtt                                                                        <  1:362906/151‑1 (MQ=255)                                                                          ggAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGt                                                                         <  1:146883/150‑1 (MQ=255)                                                                              tCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGcc                                                                    >  1:592643/1‑151 (MQ=255)                                                                               cAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCt                                                                   <  1:593328/151‑1 (MQ=255)                                                                               cAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCt                                                                   <  1:178335/151‑1 (MQ=255)                                                                                aaTGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTg                                                                  <  2:84717/151‑1 (MQ=255)                                                                                          aaCCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGaa                                                         <  2:155303/150‑1 (MQ=255)                                                                                          aaCCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGaa                                                         <  1:629200/150‑1 (MQ=255)                                                                                          aaCCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGa                                                          <  2:1057558/149‑1 (MQ=255)                                                                                                  gCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATccc                                                 <  1:683039/150‑1 (MQ=255)                                                                                                  gCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATccc                                                 <  1:986639/150‑1 (MQ=255)                                                                                                    cGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAg                                              <  2:426365/151‑1 (MQ=255)                                                                                                     gACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGc                                             >  1:90447/1‑151 (MQ=255)                                                                                                       cgcgTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCAt                                           >  2:695360/1‑151 (MQ=255)                                                                                                         cgTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCa                                         <  2:84846/151‑1 (MQ=255)                                                                                                         cgTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGCGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATc                                          >  1:119310/1‑150 (MQ=255)                                                                                                           tGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAAc                                       >  2:83620/1‑151 (MQ=255)                                                                                                              aGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGtt                                    <  1:241432/151‑1 (MQ=255)                                                                                                               gAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGttt                                   >  2:996553/1‑151 (MQ=255)                                                                                                               gAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGttt                                   <  1:1034762/151‑1 (MQ=255)                                                                                                                 aGTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCt                                 <  1:949045/151‑1 (MQ=255)                                                                                                                  gTCATGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTa                                <  2:568495/151‑1 (MQ=255)                                                                                                                     aTGGACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAac                             <  1:783263/151‑1 (MQ=255)                                                                                                                        gACGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACAcc                          <  1:819106/151‑1 (MQ=255)                                                                                                                         aCGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCg                         >  2:408135/1‑151 (MQ=255)                                                                                                                          cGTAGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGt                        <  2:358882/151‑1 (MQ=255)                                                                                                                             aGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCt                      <  1:1045578/150‑1 (MQ=255)                                                                                                                             aGCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTg                     >  1:253421/1‑151 (MQ=255)                                                                                                                              gCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTgg                    >  1:31808/1‑151 (MQ=255)                                                                                                                              gCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTgg                    <  1:516564/151‑1 (MQ=255)                                                                                                                              gCAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTgg                    >  1:516791/1‑151 (MQ=255)                                                                                                                               cAAACGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGa                   <  1:436341/151‑1 (MQ=255)                                                                                                                                aaaCGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGAc                  <  2:242396/151‑1 (MQ=255)                                                                                                                                  aCGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGt                >  1:758893/1‑151 (MQ=255)                                                                                                                                   cGCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGtt               >  1:69406/1‑151 (MQ=255)                                                                                                                                    gCCGTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGtt               >  2:389355/1‑150 (MQ=255)                                                                                                                                       gTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTc                       <  2:159176/139‑1 (MQ=255)                                                                                                                                       gTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTc                       >  1:159181/1‑139 (MQ=255)                                                                                                                                       gTTCTGGATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGt           >  1:922840/1‑151 (MQ=255)                                                                                                                                            ggATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTc         <  1:418906/148‑1 (MQ=255)                                                                                                                                            ggATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCg        <  2:51081/149‑1 (MQ=255)                                                                                                                                            ggATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCg        <  1:500052/149‑1 (MQ=255)                                                                                                                                            ggATGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCg        <  2:129998/149‑1 (MQ=255)                                                                                                                                              aTGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACaa    <  2:330566/151‑1 (MQ=255)                                                                                                                                              aTGGTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACa     >  1:634780/1‑150 (MQ=255)                                                                                                                                                ggTGCTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAAtg  <  1:232500/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    TCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTACTGACGCTGTGATGCTGTCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATG  >  NC_000913/1938436‑1938729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑