Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,130,545	G→A	R112C (CGC→TGC)	wcaD ←	putative colanic acid polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,130,545	0	G	A	100.0%	289.3 / NA	72	R112C (CGC→TGC)	wcaD	putative colanic acid polymerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/41);  total (31/41)

TTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCG  >  NC_000913/2130397‑2130691                                                                                                                                                     |                                                                                                                                                   ttCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAAt                                                                                                                                                  >  1:720944/1‑151 (MQ=255)   cAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATgg                                                                                                                                                >  2:630055/1‑151 (MQ=255)   cAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATg                                                                                                                                                 <  2:1039599/150‑1 (MQ=255)    aGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGt                                                                                                                                               >  2:767744/1‑151 (MQ=255)         aTAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTtgtgt                                                                                                                                          >  1:805000/1‑151 (MQ=255)          tAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTtgtgtg                                                                                                                                         >  1:940768/1‑151 (MQ=255)           aGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTgg                                                                                                                                        <  1:899360/151‑1 (MQ=255)            gCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGc                                                                                                                                       <  2:19188/151‑1 (MQ=255)               gTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGaa                                                                                                                                    <  2:966414/151‑1 (MQ=255)               gTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGaa                                                                                                                                    <  1:486515/151‑1 (MQ=255)                  gCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAAtt                                                                                                                                 <  2:926444/151‑1 (MQ=255)                     gTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGAGTGGCGAAATTg                                                                                                                                <  1:809792/149‑1 (MQ=255)                       aaaTCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGtttt                                                                                                                            >  2:22461/1‑151 (MQ=255)                       aaaTCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGtttt                                                                                                                            <  1:325015/151‑1 (MQ=255)                       aaaTCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGtttt                                                                                                                            <  2:489112/151‑1 (MQ=255)                       aaaTCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGtttt                                                                                                                            >  2:694881/1‑151 (MQ=255)                           cAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTaaa                                                                                                                         >  2:317038/1‑150 (MQ=255)                           cAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTaaa                                                                                                                         >  2:54133/1‑150 (MQ=255)                            aGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAAc                                                                                                                       >  1:144039/1‑151 (MQ=255)                              atatTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACg                                                                                                                      >  2:621195/1‑150 (MQ=255)                              atatTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGa                                                                                                                     <  1:245621/151‑1 (MQ=255)                                   tCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACt                                                                                                                >  1:601914/1‑151 (MQ=255)                                    cGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACt                                                                                                                <  2:458265/150‑1 (MQ=255)                                    cGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACt                                                                                                                <  1:82069/150‑1 (MQ=255)                                      aaaTCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTcc                                                                                                              >  1:365027/1‑150 (MQ=255)                                        aTCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCcac                                                                                                            >  1:264680/1‑150 (MQ=255)                                         tCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCacac                                                                                                          >  1:346101/1‑151 (MQ=255)                                            aCTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCAt                                                                                                       <  2:244820/151‑1 (MQ=255)                                            aCTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCAt                                                                                                       >  1:802512/1‑151 (MQ=255)                                            aCTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCAt                                                                                                       <  2:169569/151‑1 (MQ=255)                                               ccATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGcc                                                                                                    <  1:970367/151‑1 (MQ=255)                                                cATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGcc                                                                                                    >  2:1011765/1‑150 (MQ=255)                                                 ataataCTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCaa                                                                                                  <  2:740384/151‑1 (MQ=255)                                                 ataataCTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCa                                                                                                   >  2:841450/1‑150 (MQ=255)                                                   aataCTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCaata                                                                                                <  2:752511/151‑1 (MQ=255)                                                      aCTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATg                                                                                             <  1:376688/151‑1 (MQ=255)                                                      aCTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATg                                                                                             <  2:37779/151‑1 (MQ=255)                                                      aCTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATg                                                                                             <  2:741168/151‑1 (MQ=255)                                                         gctgcCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGa                                                                                            <  1:846153/149‑1 (MQ=255)                                                              cACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAat                                                                                     >  2:452337/1‑151 (MQ=255)                                                                  ggTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAg                                                                                 <  1:546718/151‑1 (MQ=255)                                                                    tGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGAc                                                                               >  2:402554/1‑151 (MQ=255)                                                                       ggATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACaaa                                                                            <  1:907486/151‑1 (MQ=255)                                                                         aTAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACa                                                                          <  2:139083/151‑1 (MQ=255)                                                                         aTAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACa                                                                          <  2:195942/151‑1 (MQ=255)                                                                                gtgCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGt                                                                   >  2:513865/1‑151 (MQ=255)                                                                                  gCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGtt                                                                  <  2:1015164/150‑1 (MQ=255)                                                                                  gCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTg                                                                 <  1:898276/151‑1 (MQ=255)                                                                                    cATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGaa                                                               <  1:198209/151‑1 (MQ=255)                                                                                         ccACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTg                                                            <  2:166863/149‑1 (MQ=255)                                                                                         ccACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGa                                                           <  2:361984/150‑1 (MQ=255)                                                                                         ccACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGAc                                                          <  2:952647/151‑1 (MQ=255)                                                                                             cgccgcCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTAt                                                      >  1:333694/1‑151 (MQ=255)                                                                                                cgccAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTa                                                   <  1:76518/151‑1 (MQ=255)                                                                                                         caccaccaGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATcc                                          <  1:1026239/151‑1 (MQ=255)                                                                                                                aGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACt                                   <  2:522180/151‑1 (MQ=255)                                                                                                                 gCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTg                                  <  1:699546/151‑1 (MQ=255)                                                                                                                      accaccAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCaa                             <  1:521995/151‑1 (MQ=255)                                                                                                                      accaccAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCaa                             <  2:630438/151‑1 (MQ=255)                                                                                                                           caGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAAc                           >  2:334492/1‑148 (MQ=255)                                                                                                                           caGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGa                        <  1:276303/151‑1 (MQ=255)                                                                                                                                aaaaGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGtt                   <  2:85230/151‑1 (MQ=255)                                                                                                                                 aaaGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGtt                   >  2:922360/1‑150 (MQ=255)                                                                                                                                  aaGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTa                  >  2:134057/1‑150 (MQ=255)                                                                                                                                  aaGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTa                  >  2:22276/1‑150 (MQ=255)                                                                                                                                  aaGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTa                  >  1:880236/1‑150 (MQ=255)                                                                                                                                   aGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTaaa                <  1:633200/151‑1 (MQ=255)                                                                                                                                           aaTATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTg         >  2:681537/1‑150 (MQ=255)                                                                                                                                            atatCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTg         >  1:202355/1‑149 (MQ=255)                                                                                                                                                 ttGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCcg  >  2:650258/1‑151 (MQ=255)                                                                                                                                                 ttGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCcg  >  1:234878/1‑151 (MQ=255)                                                                                                                                                  tGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCcg  <  1:767769/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   TTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCG  >  NC_000913/2130397‑2130691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑