Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,178,383	C→T	R61H (CGT→CAT)	fbaB ←	fructose‑bisphosphate aldolase class I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,178,383	0	C	T	100.0%	203.2 / NA	50	R61H (CGT→CAT)	fbaB	fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/24);  total (26/24)

CGCCACACAGTTACAGCCCGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGG  >  NC_000913/2178235‑2178530                                                                                                                                                     |                                                                                                                                                    cGCCACACAGTTACAGCCCGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGc                                                                                                                                                   <  2:335054/151‑1 (MQ=255)        caGTTACAGCCCGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGgtgt                                                                                                                                            <  1:43050/151‑1 (MQ=255)          gTTACAGCCCGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGtgttg                                                                                                                                          >  1:1012932/1‑151 (MQ=255)          gTTACAGCCCGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGtgttg                                                                                                                                          <  2:1012891/151‑1 (MQ=255)          gTTACAGCCCGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGtgttg                                                                                                                                          >  1:429889/1‑151 (MQ=255)             aCAGCCCGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTAc                                                                                                                                       >  1:671172/1‑151 (MQ=255)             aCAGCCCGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTAc                                                                                                                                       >  1:963909/1‑151 (MQ=255)               aGCCCGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACa                                                                                                                                      >  2:1027560/1‑150 (MQ=255)               aGCCCGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACa                                                                                                                                      >  1:629315/1‑150 (MQ=255)                 cccGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACg                                                                                                                                   >  1:681632/1‑151 (MQ=255)                 cccGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACg                                                                                                                                   <  1:586829/151‑1 (MQ=255)                      ttCGATCGCCAGTTCAACAATGTTTTTCGGGTCAGAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGc                                                                                                                              >  1:53201/1‑151 (MQ=255)                         gATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCata                                                                                                                           <  2:391516/151‑1 (MQ=255)                             gCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTAc                                                                                                                       <  1:391482/151‑1 (MQ=255)                               cAGTTCAACAATGTTTTTCGGGTAAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGt                                                                                                                     >  2:819678/1‑151 (MQ=255)                                 gTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTa                                                                                                                    >  1:169999/1‑150 (MQ=255)                                  ttCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCACATCAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCAACAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAac                                                                                                                  >  1:193255/1‑151 (MQ=255)                                   tCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAac                                                                                                                  >  2:749465/1‑150 (MQ=255)                                   tCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAac                                                                                                                  >  2:897450/1‑150 (MQ=255)                                    caacaaTGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAaca                                                                                                                 <  2:80516/150‑1 (MQ=255)                                         aTGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTg                                                                                                           <  2:791881/151‑1 (MQ=255)                                         aTGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTg                                                                                                           <  1:786867/151‑1 (MQ=255)                                               ttCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTggcgggc                                                                                                     >  1:660971/1‑151 (MQ=255)                                                tCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTggcgggcg                                                                                                    >  2:764079/1‑151 (MQ=255)                                                 cGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTggcgggcg                                                                                                    >  1:33864/1‑150 (MQ=255)                                                   ggTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAGCGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGatt                                                                                                 <  1:434345/151‑1 (MQ=255)                                                       aaaGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTg                                                                                             <  1:973717/151‑1 (MQ=255)                                                             gagCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATc                                                                                       >  1:499207/1‑151 (MQ=255)                                                             gagCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATc                                                                                       <  2:345106/151‑1 (MQ=255)                                                              agCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCa                                                                                      <  1:203676/151‑1 (MQ=255)                                                                         agcaAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCt                                                                           <  2:161983/151‑1 (MQ=255)                                                                             aaTGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGt                                                                       >  1:666342/1‑151 (MQ=255)                                                                              aTGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTACCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTa                                                                      <  1:39034/151‑1 (MQ=255)                                                                                gAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGt                                                                    <  1:969067/151‑1 (MQ=255)                                                                                gAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGt                                                                    <  1:316954/151‑1 (MQ=255)                                                                                      ccGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATg                                                                <  2:595631/149‑1 (MQ=255)                                                                                       cGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTcc                                                             >  1:49893/1‑151 (MQ=255)                                                                                          cAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCggg                                                          >  1:136111/1‑151 (MQ=255)                                                                                          cAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCggg                                                          >  1:990285/1‑151 (MQ=255)                                                                                               tGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCgggg                                                         >  2:426551/1‑147 (MQ=255)                                                                                                gCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGAt                                                     <  1:638753/150‑1 (MQ=255)                                                                                                    aaCGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGc                                                <  1:747610/151‑1 (MQ=255)                                                                                                       gCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTgg                                             >  2:357781/1‑151 (MQ=255)                                                                                                        cccTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGt                                            <  2:295708/151‑1 (MQ=255)                                                                                                                aaCCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAgg                                     >  2:656524/1‑150 (MQ=255)                                                                                                                  ccGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAAt                                  >  2:270597/1‑151 (MQ=255)                                                                                                                            aGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACaa                        <  2:448002/151‑1 (MQ=255)                                                                                                                              aaaGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACg                      <  2:1044645/151‑1 (MQ=255)                                                                                                                                 gATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTg                   <  1:863906/151‑1 (MQ=255)                                                                                                                                                  agATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCgg  >  1:475583/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                    CGCCACACAGTTACAGCCCGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGG  >  NC_000913/2178235‑2178530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑