Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,366,486 G→A A187T (GCT→ACT)  arnB → uridine 5'‑(beta‑1‑threo‑pentapyranosyl‑4‑ulose diphosphate) aminotransferase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,366,4860GA98.6% 262.7 / NA 69A187T (GCT→ACT) arnBuridine 5'‑(beta‑1‑threo‑pentapyranosyl‑4‑ulose diphosphate) aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (36/32);  total (36/33)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGACGCCATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTAT  >  NC_000913/2366338‑2366629
                                                                                                                                                    |                                                                                                                                               
tGACGCCATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACt                                                                                                                                               >  2:651435/1‑151 (MQ=255)
      cATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGt                                                                                                                                         <  1:511818/151‑1 (MQ=255)
           gcgcCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCt                                                                                                                                    >  1:645489/1‑151 (MQ=255)
            cgcCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTg                                                                                                                                   <  2:604/151‑1 (MQ=255)
             gcCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGa                                                                                                                                  <  2:725559/151‑1 (MQ=255)
                aTTGGCGAACGTTACGGCATCGCAGTAAACGAAGATGCTGCCCATACCGAGGGTACGTATTACAAAGCTCGCTATATTGTCGCCAAAGGTACCGCTATTTTTTCGTTTCATGCCATTAAAAAGATTACCTGTACTGAAGGTGGCCTGATTg                                                                                                                               <  2:854236/151‑1 (MQ=255)
                   ggCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTaa                                                                                                                            <  1:1039529/151‑1 (MQ=255)
                   ggCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTaa                                                                                                                            <  1:754059/151‑1 (MQ=255)
                    gCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAAc                                                                                                                           >  1:1034794/1‑151 (MQ=255)
                     cGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACt                                                                                                                          >  2:476191/1‑151 (MQ=255)
                        aCGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGAt                                                                                                                       <  2:383500/151‑1 (MQ=255)
                        aCGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGAt                                                                                                                       <  2:266809/151‑1 (MQ=255)
                               ggCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGaa                                                                                                                 >  2:771346/1‑150 (MQ=255)
                                gCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGaaa                                                                                                                >  1:409224/1‑150 (MQ=255)
                                 cATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAAc                                                                                                              >  2:506134/1‑151 (MQ=255)
                                   tCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCt                                                                                                            >  2:1036366/1‑151 (MQ=255)
                                    cGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCtt                                                                                                           <  2:707318/151‑1 (MQ=255)
                                    cGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCtt                                                                                                           <  2:990124/151‑1 (MQ=255)
                                                    ggtgcCCATACCGTCGGTACGTATTCCAAAGGGCGACATATTGCCTCAAAAGGTCCCGCTATTTTTTCATTTCATGTCATTAAAAAGATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAGCCTTGCCCGCCAACTa                                                                                               <  2:466677/145‑1 (MQ=255)
                                                         ccATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTg                                                                                      >  1:944385/1‑151 (MQ=255)
                                                             gCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAAt                                                                                  >  2:333414/1‑151 (MQ=255)
                                                             gCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAAt                                                                                  <  1:812460/151‑1 (MQ=255)
                                                                  cGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCAc                                                                             >  2:183737/1‑151 (MQ=255)
                                                                    gTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACgg                                                                           >  1:772891/1‑151 (MQ=255)
                                                                     tACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGt                                                                          >  2:234192/1‑151 (MQ=255)
                                                                      aCGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTc                                                                         >  2:914357/1‑151 (MQ=255)
                                                                      aCGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGCAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGt                                                                          <  2:476233/150‑1 (MQ=255)
                                                                       cGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCt                                                                        <  2:719902/151‑1 (MQ=255)
                                                                         tATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTgg                                                                      >  1:925882/1‑151 (MQ=255)
                                                                            tACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGgtg                                                                   >  2:152713/1‑151 (MQ=255)
                                                                             aCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGgtgt                                                                  <  1:957872/151‑1 (MQ=255)
                                                                             aCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGgtgt                                                                  <  2:815309/151‑1 (MQ=255)
                                                                             aCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGgtg                                                                   <  1:554513/150‑1 (MQ=255)
                                                                                 aGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGAt                                                              <  1:183744/151‑1 (MQ=255)
                                                                                 aGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGAt                                                              <  1:914393/151‑1 (MQ=255)
                                                                                       aCATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTa                                                         <  2:957956/150‑1 (MQ=255)
                                                                                              ggCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATgacaga                                                  <  1:743839/150‑1 (MQ=255)
                                                                                               gcgcAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATgacagac                                                 >  1:646910/1‑150 (MQ=255)
                                                                                                cgcAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATgacagaca                                                >  1:586636/1‑150 (MQ=255)
                                                                                                   aaaaGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGGTAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAAcc                                            >  1:986398/1‑151 (MQ=255)
                                                                                                   aaaaGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAAcc                                            >  1:629369/1‑151 (MQ=255)
                                                                                                   aaaaGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAAcc                                            <  2:883466/151‑1 (MQ=255)
                                                                                                   aaaaGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAAc                                             <  1:364366/150‑1 (MQ=255)
                                                                                                     aaGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTg                                          >  1:664013/1‑151 (MQ=255)
                                                                                                     aaGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCGACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTg                                          <  2:1006845/151‑1 (MQ=255)
                                                                                                        gTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTgggg                                       >  2:373564/1‑151 (MQ=255)
                                                                                                          aCCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGcc                                     >  1:594816/1‑151 (MQ=255)
                                                                                                          aCCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGc                                      <  1:964455/150‑1 (MQ=255)
                                                                                                           ccGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCg                                    >  1:1061397/1‑151 (MQ=255)
                                                                                                            cGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGt                                   >  2:729178/1‑151 (MQ=255)
                                                                                                             gCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTg                                  <  1:112583/151‑1 (MQ=255)
                                                                                                                aTTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCAc                               >  2:561964/1‑151 (MQ=255)
                                                                                                                  tttttCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCAcc                              <  2:832789/150‑1 (MQ=255)
                                                                                                                   ttttCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCg                             <  2:548275/150‑1 (MQ=255)
                                                                                                                   ttttCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGc                            >  2:892019/1‑151 (MQ=255)
                                                                                                                    tttcatttcaTGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGc                            >  2:731353/1‑150 (MQ=255)
                                                                                                                     ttcatttcatGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGc                            >  2:997024/1‑149 (MQ=255)
                                                                                                                       catttcatGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGc                        >  2:180449/1‑151 (MQ=255)
                                                                                                                       catttcatGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGc                        >  1:585121/1‑151 (MQ=255)
                                                                                                                        atttcatGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCt                       >  1:255605/1‑151 (MQ=255)
                                                                                                                          ttcatGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTg                      >  2:192757/1‑150 (MQ=255)
                                                                                                                            catGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAg                   <  2:640391/151‑1 (MQ=255)
                                                                                                                            catGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAg                   <  2:266716/151‑1 (MQ=255)
                                                                                                                                   ttAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTaa             >  1:469920/1‑150 (MQ=255)
                                                                                                                                       aaaTATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACAcc         >  2:733529/1‑150 (MQ=255)
                                                                                                                                        aaTATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCgg       <  2:1022566/151‑1 (MQ=255)
                                                                                                                                         atatTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCggg      <  1:622331/151‑1 (MQ=255)
                                                                                                                                              aCCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCtat  >  2:461213/1‑150 (MQ=255)
                                                                                                                                               ccTGTACTGAAGGTGGCCTGATTGTACCTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAGCCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCtat  <  1:212948/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
TGACGCCATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTAT  >  NC_000913/2366338‑2366629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑