Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,380,055	T→C	T197A (ACT→GCT)	menF ←	isochorismate synthase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,380,055	0	T	C	100.0%	275.5 / NA	74	T197A (ACT→GCT)	menF	isochorismate synthase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/35);  total (39/35)

CGCCGCCATAACCGTTCCGGTGAAGAGCCAAGAAAAGCATTTTCGCCATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGTTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAATTGCGTCCAGCCCGTTTTGTCCGGCCAGTGTTGTTCTCGCGTGGTGGTTAAATGTAACCCAGGCAAGGGCTTGATACTCAC  >  NC_000913/2379910‑2380202                                                                                                                                                  |                                                                                                                                                    cgccgccATAACCGTTCCGGTGAAGAGCCAAGAAAAGCATTTTCGCCATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGccc                                                                                                                                                <  2:544392/151‑1 (MQ=255) cgccgccATAACCGTTCCGGTGAAGAGCCAAGAAAAGCATTTTCGCCATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGccc                                                                                                                                                >  2:350701/1‑151 (MQ=255) cgccgccATAACCGTTCCGGTGAAGAGCCAAGAAAAGCATTTTCGCCATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGccc                                                                                                                                                >  1:488266/1‑151 (MQ=255)   ccgccATAACCGTTCCGGTGAAGAGCCAAGAAAAGCATTTTCGCCATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCcg                                                                                                                                               >  2:150812/1‑150 (MQ=255)     gccATAACCGTTCCGGTGAAGAGCCAAGAAAAGCATTTTCGCCATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCcgagc                                                                                                                                            <  2:407675/151‑1 (MQ=255)       cATAACCGTTCCGGTGAAGAGCCAAGAAAAGCATTTTCGCCATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCcgagcg                                                                                                                                           >  1:346467/1‑150 (MQ=255)           aCCGTTCCGGTGAAGAGCCAAGAAAAGCATTTTCGCCATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGcac                                                                                                                                      >  2:978029/1‑151 (MQ=255)           aCCGTTCCGGTGAAGAGCCAAGAAAAGCATTTTCGCCATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGcac                                                                                                                                      <  2:682726/151‑1 (MQ=255)                  cGGTGAAGAGCCAAGAAAAGCATTTTCGCCATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTg                                                                                                                               >  1:842664/1‑151 (MQ=255)                          agCCAAGAAAAGCATTTTCGCCATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTc                                                                                                                       <  2:409627/151‑1 (MQ=255)                           gCCAAGAAAAGCATTTTCGCCATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTc                                                                                                                       <  1:645709/150‑1 (MQ=255)                           gCCAAGAAAAGCATTTTCGCCATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCa                                                                                                                      <  1:776407/151‑1 (MQ=255)                                 aaaaGCATTTTCGCCATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCATCACTTTGTCGAGCTCACCTTCg                                                                                                                <  1:362401/151‑1 (MQ=255)                                  aaaGCATTTTCGCCATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCgg                                                                                                               >  1:354339/1‑151 (MQ=255)                                   aaGCATTTTCGCCATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGc                                                                                                              >  1:974136/1‑151 (MQ=255)                                     gCATTTTCGCCATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCg                                                                                                             >  2:1055486/1‑150 (MQ=255)                                     gCATTTTCGCCATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCg                                                                                                             >  2:899182/1‑150 (MQ=255)                                      cATTTTCGCCATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGAt                                                                                                           <  1:751573/151‑1 (MQ=255)                                       aTTTTCGCCATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATg                                                                                                          >  2:867294/1‑151 (MQ=255)                                        ttttCGCCATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATgg                                                                                                         >  2:801241/1‑151 (MQ=255)                                          ttCGCCATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGtt                                                                                                       >  2:865500/1‑151 (MQ=255)                                          ttCGCCATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGt                                                                                                        <  2:416972/150‑1 (MQ=255)                                          ttCGCCATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGt                                                                                                        <  2:522592/150‑1 (MQ=255)                                           tggacATCAAAGGCCACGTAAAAAAAATAGCAATTCAGATTCAGGCGACGACTGGCAGCCATCAGCCACGCCGCGATGACCGGACTTGCGAACTGCAGGGAAGCTGCCCGAGCGAGCACCACTTTATCGAGCTCACCTTCGGCGATGGatt                                                                                                      >  2:519978/5‑151 (MQ=255)                                           tCGCCATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGttt                                                                                                      >  2:240435/1‑151 (MQ=255)                                            cGCCATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGtttt                                                                                                     <  1:822080/151‑1 (MQ=255)                                             gCCATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTc                                                                                                    <  1:63925/151‑1 (MQ=255)                                             gCCATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTc                                                                                                    <  2:518560/151‑1 (MQ=255)                                              ccATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCg                                                                                                   >  2:1042975/1‑151 (MQ=255)                                               cATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGt                                                                                                  <  1:1057867/151‑1 (MQ=255)                                               cATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGt                                                                                                  >  2:268054/1‑151 (MQ=255)                                                 tCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTg                                                                                                >  2:441481/1‑151 (MQ=255)                                                     aGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAg                                                                                            <  2:164914/151‑1 (MQ=255)                                                      ggCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGt                                                                                           <  1:514493/151‑1 (MQ=255)                                                       gCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGtt                                                                                          <  2:903730/151‑1 (MQ=255)                                                        ccATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTc                                                                                         >  1:82590/1‑151 (MQ=255)                                                         cATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCg                                                                                        <  2:716045/151‑1 (MQ=255)                                                         cATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCg                                                                                        >  2:136891/1‑151 (MQ=255)                                                          aTGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCg                                                                                        >  1:585534/1‑150 (MQ=255)                                                            gTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGAtt                                                                                     >  1:292448/1‑151 (MQ=255)                                                             tAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGAtt                                                                                     >  1:123188/1‑150 (MQ=255)                                                              aaaaaTGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTa                                                                                    >  1:999991/1‑150 (MQ=255)                                                               aaaaTGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAAt                                                                                  >  2:366844/1‑151 (MQ=255)                                                               aaaaTGGCAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAAt                                                                                  >  1:366105/1‑151 (MQ=255)                                                                 aaTGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAATTg                                                                                <  1:243440/151‑1 (MQ=255)                                                                     gTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAATTGCGTc                                                                            <  1:487712/151‑1 (MQ=255)                                                                       aGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAATTGCGTCCa                                                                          <  2:27313/151‑1 (MQ=255)                                                                         cAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAATTGCGTCCAGc                                                                        >  1:598453/1‑151 (MQ=255)                                                                          aaTTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAATTGCGTCCAGcc                                                                       >  1:706894/1‑151 (MQ=255)                                                                          aaTTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAATTGCGTCCAGcc                                                                       >  1:138222/1‑151 (MQ=255)                                                                              cagattcagTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAATTGCGTCCAGCCCGtt                                                                   <  1:861886/151‑1 (MQ=255)                                                                              cagattcagTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAATTGCGTCCAGCCCGt                                                                    <  2:902989/150‑1 (MQ=255)                                                                               agattcagTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAATTGCGTCCAGCCCGttt                                                                  >  2:759441/1‑151 (MQ=255)                                                                                      gTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAATTGCGTCCAGCCCGTTTTGTCCgg                                                           >  1:1056571/1‑151 (MQ=255)                                                                                           cgacTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAATTGCGTCCAGCCCGTTTTGTCCCGCCAgt                                                      >  1:642820/1‑151 (MQ=255)                                                                                                ggCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAATTGCGTCCAGCCCGTTTTGTCCGGCCAGtgttgt                                                 <  1:984413/151‑1 (MQ=255)                                                                                                  cAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAATTGCGTCCAGCCCGTTTTGTCCGGCCAGTGTTGTtc                                               >  2:57428/1‑151 (MQ=255)                                                                                                   aGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAATTGCGTCCAGCCCGTTTTGTCCGGCCAGTGTTGTtct                                              <  1:640302/151‑1 (MQ=255)                                                                                                              ccgccgcGTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAATTGCGTCCAGCCCGTTTTGTCCGGCCAGTGTTGTTCTCGCGTgggggt                                   >  2:1027873/1‑151 (MQ=255)                                                                                                                  cgcgTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAATTGCGTCCAGCCCGTTTTGTCCGGCCAGTGTTGTTCTCGCGTGGTGGTTaaa                               <  1:828691/151‑1 (MQ=255)                                                                                                                   gcgTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAATTGCGTCCAGCCCGTTTTGTCCGGCCAGTGTTGTTCTCGCGTGGTGGTTAAAt                              <  1:523871/151‑1 (MQ=255)                                                                                                                   gcgTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAATTGCGTCCAGCCCGTTTTGTCCGGCCAGTGTTGTTCTCGCGTGGTGGTTAAAt                              <  1:724763/151‑1 (MQ=255)                                                                                                                     gTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAATTGCGTCCAGCCCGTTTTGTCCGGCCAGTGTTGTTCTCGCGTGGTGGTTAAATGt                            >  2:509075/1‑151 (MQ=255)                                                                                                                     gTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAATTGCGTCCAGCCCGTTTTGTCCGGCCAGTGTTGTTCTCGCGTGGTGGTTAAATGt                            <  1:80541/151‑1 (MQ=255)                                                                                                                     gTTGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACATTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAATTGCGTCCAGCCCGTTTTGTCCGGCCAGTGTTGTTCTCGCGTGGTGGTTAAAt                              <  2:763911/149‑1 (MQ=255)                                                                                                                      ttGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAATTGCGTCCAGCCCGTTTTGTCCGGCCAGTGTTGTTCTCGCGTGGTGGTTAAATGt                            >  1:923786/1‑150 (MQ=255)                                                                                                                       tGACCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAATTGCGTCCAGCCCGTTTTGTCCGGCCAGTGTTGTTCTCGCGTGGTGGTTAAATGTa                           <  1:663557/150‑1 (MQ=255)                                                                                                                         aCCGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAATTGCGTCCAGCCCGTTTTGTCCGGCCAGTGTTGTTCTCGCGTGGTGGTTAAATGTAAcc                        >  1:530592/1‑151 (MQ=255)                                                                                                                          ccGGACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAATTGCGTCCAGCCCGTTTTGTCCGGCCAGTGTTGTTCTCGCGTGGTGGTTAAATGTAAcc                        <  2:45601/150‑1 (MQ=255)                                                                                                                            ggACTTGCGAAATGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAATTGCGTCCAGCCCGTTTTGTCCGGCCAGTGTTGTTCTCGCGTGGTGGTTAAATGTAACCCAg                     <  1:616862/151‑1 (MQ=255)                                                                                                                             gACTTGCGAAATGCCGGTCAGCTGCCCGAGCGCGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCTCGTTCGATTAATTGCGTCCAGCCCGTTTTGTCCGGCCAGTGTTGTTCTCGCGTGGTGGTTAAATGTAACCCAgg                    <  1:513384/151‑1 (MQ=255)                                                                                                                                      aaTGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAATTGCGTCCAGCCCGTTTTGTCCGGCCAGTGTTGTTCTCGCGTGGTGGTTAAATGTAACCCAGGCAAGGGCtt           <  1:441492/151‑1 (MQ=255)                                                                                                                                       aTGCAGGTCAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAATTGCGTCCAGCCCGTTTTGTCCGGCCAGTGTTGTTCTCGCGTGGTGGTTAAATGTAACCCAGGCAAGGGCTTg          >  1:140973/1‑151 (MQ=255)                                                                                                                                               cAGCTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAATTGCGTCCAGCCCGTTTTGTCCGGCCAGTGTTGTTCTCGCGTGGTGGTTAAATGTAACCCAGGCAAGGGCTTGATACTCAc  >  2:386489/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                                    CGCCGCCATAACCGTTCCGGTGAAGAGCCAAGAAAAGCATTTTCGCCATCAAAGGCCATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGTTGCCCGAGCGAGCACCACTTTGTCGAGCTCACCTTCGGCGATGGTTTTCGTTGCCAGTTCGATTAATTGCGTCCAGCCCGTTTTGTCCGGCCAGTGTTGTTCTCGCGTGGTGGTTAAATGTAACCCAGGCAAGGGCTTGATACTCAC  >  NC_000913/2379910‑2380202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑