Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	158,903	C→T	G75D (GGC→GAC)	pcnB ←	poly(A) polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	158,903	0	C	T	100.0%	209.5 / NA	54	G75D (GGC→GAC)	pcnB	poly(A) polymerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/23);  total (31/23)

GGAAGGTCGCAACTTCGATAATCTCCGGGCCAAACATTACATGAGCCAGACGGAAACGGCGACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGCCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGAC  >  NC_000913/158757‑159050                                                                                                                                                   |                                                                                                                                                    ggAAGGTCGCAACTTCGATAATCTCCGGGCCAAACATTACATGAGCCAGACGGAAACGGCGACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTTGGCTTTTTGTCa                                                                                                                                                   >  1:11800/1‑149 (MQ=255)  gAAGGTCGCAACTTCGATAATCTCCGGGCCAAACATTACATGAGCCAGACGGAAACGGCGACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGt                                                                                                                                                >  2:567912/1‑151 (MQ=255)       tCGCAACTTCGATAATCTCCGGGCCAAACATTACATGAGCCAGACGGAAACGGCGACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAAc                                                                                                                                             >  2:21698/1‑149 (MQ=255)         gCAACTTCGATAATCTCCGGGCCAAACATTACATGAGCCAGACGGAAACGGCGACCCACCAGGCGGCAGTTACGTAACAGTTTGCGCACCTGCCCAGCCCCGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGCAACAg                                                                                                                                           >  2:453023/1‑149 (MQ=255)           aaCTTCGATAATCTCCGGGCCAAACATTACATGAGCCAGACGGAAACGGCGACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTcg                                                                                                                                       >  2:987944/1‑151 (MQ=255)           aaCTTCGATAATCTCCGGGCCAAACATTACATGAGCCAGACGGAAACGGCGACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTcg                                                                                                                                       >  2:378077/1‑151 (MQ=255)                cGATAATCTCCGGGCCAAACATTACATGAGCCAGACGGAAACGGCGACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCAc                                                                                                                                  <  1:157826/151‑1 (MQ=255)                   tAATCTCCGGGCCAAACATTACATGAGCCAGACGGAAACGGCGACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCAcgcc                                                                                                                               >  2:755556/1‑151 (MQ=255)                    aaTCTCCGGGCCAAACATTACATGAGCCAGACGGAAACGGCGACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCAcgccg                                                                                                                              >  2:649987/1‑151 (MQ=255)                            ggCCAAACATTACATGAGCCAGACGGAAACGGCGACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAAc                                                                                                                      >  2:1010502/1‑151 (MQ=255)                              ccAAACATTACATGAGCCAGACGGAAACGGCGACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAAcca                                                                                                                    <  1:412526/151‑1 (MQ=255)                                  aCATTACATGAGCCAGACGGAAACGGCGACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAAccagcca                                                                                                                <  2:354302/151‑1 (MQ=255)                                  aCATTACATGAGCCAGACGGAAACGGCGACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAAccagcc                                                                                                                 >  2:344838/1‑150 (MQ=255)                                   cATTACATGAGCCAGACGGAAACGGCGACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAAccagcc                                                                                                                 >  1:715714/1‑149 (MQ=255)                                        cATGAGCCAGACGGAAACGGCGACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTc                                                                                                          >  1:379967/1‑151 (MQ=255)                                               ccgACGGAAACGGCGACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATccc                                                                                                   >  1:77308/3‑151 (MQ=255)                                                   cGGAAACGGCGACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCTTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCtt                                                                                               <  2:943247/151‑1 (MQ=255)                                                   cGGAAACGGCGACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCtt                                                                                               <  1:1015133/151‑1 (MQ=255)                                                    ggAAACGGCGACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCttt                                                                                              <  1:451825/151‑1 (MQ=255)                                                      aaaCGGCGACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTAt                                                                                            >  2:748515/1‑151 (MQ=255)                                                       aaCGGCGACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTAtt                                                                                           <  2:853134/151‑1 (MQ=255)                                                        aCGGCGACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTg                                                                                          <  1:257113/151‑1 (MQ=255)                                                         cGGCGACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGa                                                                                         <  1:596399/151‑1 (MQ=255)                                                             gACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCt                                                                                     >  1:45442/1‑151 (MQ=255)                                                               cccACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGt                                                                                   >  1:417904/1‑151 (MQ=255)                                                                ccaccaGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGt                                                                                   >  2:898283/1‑150 (MQ=255)                                                                   ccaGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACAt                                                                               <  1:1023312/151‑1 (MQ=255)                                                                     aGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACAtt                                                                              >  1:236381/1‑150 (MQ=255)                                                                         ggcAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCtt                                                                         >  2:447338/1‑151 (MQ=255)                                                                            aGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAg                                                                      <  1:965729/151‑1 (MQ=255)                                                                                 cGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCAt                                                                 <  2:396525/151‑1 (MQ=255)                                                                                          ttGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTg                                                         <  2:314474/150‑1 (MQ=255)                                                                                            gcgcACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGata                                                      <  2:789957/151‑1 (MQ=255)                                                                                              gcACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATc                                                    <  1:1025326/151‑1 (MQ=255)                                                                                              gcACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATc                                                    >  1:197795/1‑151 (MQ=255)                                                                                                 ccTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCttt                                                 >  1:959543/1‑151 (MQ=255)                                                                                                     cTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCgg                                             >  2:896415/1‑151 (MQ=255)                                                                                                       cAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCggg                                            >  1:653876/1‑150 (MQ=255)                                                                                                        aGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGa                                           >  1:470494/1‑150 (MQ=255)                                                                                                             tggcgtTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAATAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTACGGGAAATAGc                                     >  2:373838/1‑151 (MQ=255)                                                                                                               gcgtTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCAt                                   <  2:724023/151‑1 (MQ=255)                                                                                                                cgtTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCAtg                                  <  1:584140/151‑1 (MQ=255)                                                                                                                      tGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTc                            >  1:611428/1‑151 (MQ=255)                                                                                                                        gTTACGGCAAAACATTGCCGCTTTTTGTCAACGACCCGGGCGGGCAAGCCGCCGCCAAGCAGGCAGGTTTTTTATCCCGCTTTTTTTAGCCTTTACATTTTCATCAGGGCCTTTTCACTGATATCTTTGCGGGAAATAGCATgctgct                             <  2:691690/148‑1 (MQ=255)                                                                                                                         ttACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCAcg                         >  2:27922/1‑151 (MQ=255)                                                                                                                          tACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCAcgc                        >  1:183027/1‑151 (MQ=255)                                                                                                                            cGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCgg                      >  1:492937/1‑151 (MQ=255)                                                                                                                             gTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCggg                     <  2:602237/151‑1 (MQ=255)                                                                                                                                aaaaTCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATc                  <  1:357261/151‑1 (MQ=255)                                                                                                                                  aaTCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTCCCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCAc                <  2:438524/151‑1 (MQ=255)                                                                                                                                  aaTCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCAc                >  1:931508/1‑151 (MQ=255)                                                                                                                                       ttCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCAcc               <  1:896606/147‑1 (MQ=255)                                                                                                                                       ttCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGt             <  1:907884/149‑1 (MQ=255)                                                                                                                                                ttGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGAc  >  2:798107/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                    GGAAGGTCGCAACTTCGATAATCTCCGGGCCAAACATTACATGAGCCAGACGGAAACGGCGACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGCCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGAC  >  NC_000913/158757‑159050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑