Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,421,841	T→C	V45A (GTG→GCG)	yfcH →	putative NAD‑dependent nucleotide‑sugar epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,421,841	0	T	C	100.0%	273.9 / NA	75	V45A (GTG→GCG)	yfcH	putative NAD‑dependent nucleotide‑sugar epimerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/39);  total (36/39)

CGGGAGGCTGCATGAACATAGTGATCACCGGAGGGACAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAA  >  NC_000913/2421697‑2421986                                                                                                                                                 |                                                                                                                                                  cGGGAGGCTGCATGAACATAGTGATCACCGGAGGGACAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGacac                                                                                                                                              >  2:375827/1‑150 (MQ=255)  gggAGGCTGCATGAACATAGTGATCACCGGAGGGACAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTa                                                                                                                                            <  2:889989/151‑1 (MQ=255)                aCATAGTGATCACCGGAGGGACAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGc                                                                                                                              <  2:734163/151‑1 (MQ=255)                aCATAGTGATCACCGGAGGGACAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGc                                                                                                                              >  2:1021324/1‑151 (MQ=255)                  aTAGTGATCACCGGAGGGACAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCg                                                                                                                            <  2:896426/151‑1 (MQ=255)                  aTAGTGATCACCGGAGGGACAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCg                                                                                                                            <  1:458583/151‑1 (MQ=255)                   tAGTGATCACCGGAGGGACAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCg                                                                                                                            >  2:961409/1‑150 (MQ=255)                       gATCACCGGAGGGACAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCa                                                                                                                        >  1:578065/1‑150 (MQ=255)                       gATCACCGGAGGGACAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCa                                                                                                                        >  1:5671/1‑150 (MQ=255)                        aTCACCGGAGGGACAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCaaa                                                                                                                      <  1:855832/151‑1 (MQ=255)                        aTCACCGGAGGGACAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCaaa                                                                                                                      <  1:896056/151‑1 (MQ=255)                        aTCACCGGAGGGACAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCaaa                                                                                                                      <  2:1033695/151‑1 (MQ=255)                               gAGGGACAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCt                                                                                                               <  2:595319/151‑1 (MQ=255)                                aGGGACAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTc                                                                                                              <  1:461929/151‑1 (MQ=255)                                 gggACAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTc                                                                                                              <  1:621523/150‑1 (MQ=255)                                    aCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACg                                                                                                          >  1:697681/1‑151 (MQ=255)                                         aTTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGtt                                                                                                     <  1:558803/151‑1 (MQ=255)                                         aTTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGtt                                                                                                     <  1:938454/151‑1 (MQ=255)                                             aTTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATg                                                                                                 >  1:321984/1‑151 (MQ=255)                                              ttGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGc                                                                                                >  2:1055925/1‑151 (MQ=255)                                                ggTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGTCACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCgg                                                                                              >  2:1025527/1‑151 (MQ=255)                                                ggTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCgg                                                                                              >  2:361248/1‑151 (MQ=255)                                                ggTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCgg                                                                                              >  1:624005/1‑151 (MQ=255)                                                 gTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGt                                                                                             <  2:683534/151‑1 (MQ=255)                                                     ccATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGCCGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTa                                                                                            <  2:363453/148‑1 (MQ=255)                                                     ccATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAAt                                                                                          <  1:485021/150‑1 (MQ=255)                                                        tttGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAAc                                                                                      >  2:253858/1‑151 (MQ=255)                                                            aTTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTgg                                                                                  >  1:354927/1‑151 (MQ=255)                                                             ttCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGc                                                                                 >  1:672729/1‑151 (MQ=255)                                                               ccGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCg                                                                               >  1:504999/1‑151 (MQ=255)                                                                 gcgTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGga                                                                             <  1:23844/151‑1 (MQ=255)                                                                     ttGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAAc                                                                         >  1:239974/1‑151 (MQ=255)                                                                       gCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAAcct                                                                       <  2:65074/151‑2 (MQ=255)                                                                       gCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCg                                                                       >  2:274810/1‑151 (MQ=255)                                                                          ggAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGAt                                                                     <  2:521727/150‑1 (MQ=255)                                                                          ggAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGAt                                                                     <  1:883756/150‑1 (MQ=255)                                                                            aGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGc                                                                  >  2:494561/1‑151 (MQ=255)                                                                                    cATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAAc                                                          <  2:473801/151‑1 (MQ=255)                                                                                       cAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGc                                                        <  2:725015/150‑1 (MQ=255)                                                                                       cAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCt                                                       <  2:369431/151‑1 (MQ=255)                                                                                       cAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCt                                                       >  1:77133/1‑151 (MQ=255)                                                                                        aaaTTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTg                                                      >  2:177067/1‑151 (MQ=255)                                                                                           ttACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGAc                                                   >  2:511724/1‑151 (MQ=255)                                                                                            tACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGAc                                                   <  1:791170/150‑1 (MQ=255)                                                                                            tACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGAc                                                   <  1:790245/150‑1 (MQ=255)                                                                                             aCGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTc                                                 >  1:377645/1‑151 (MQ=255)                                                                                               ggTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCAc                                               >  2:1006400/1‑151 (MQ=255)                                                                                                gTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACg                                              <  2:390599/151‑1 (MQ=255)                                                                                                  aGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACg                                              >  2:362482/1‑149 (MQ=255)                                                                                                     gACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCa                                          >  1:557926/1‑150 (MQ=255)                                                                                                        gcgTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTAGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCaaaaa                                      <  2:194353/151‑1 (MQ=255)                                                                                                          gTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCaaaa                                       >  1:741331/1‑148 (MQ=255)                                                                                                          gTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAaga                                    <  2:144985/151‑1 (MQ=255)                                                                                                            aaCCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGc                                  >  1:323958/1‑151 (MQ=255)                                                                                                             aCCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCg                                 <  2:930304/151‑1 (MQ=255)                                                                                                                cGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGtct                              <  1:732036/151‑1 (MQ=255)                                                                                                                 gCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGtctc                             >  1:858753/1‑151 (MQ=255)                                                                                                                  cAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTctct                            >  2:452925/1‑151 (MQ=255)                                                                                                                  cAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTctct                            >  2:141906/1‑151 (MQ=255)                                                                                                                        gCCAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCaaa                      <  2:27887/151‑1 (MQ=255)                                                                                                                         ccAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCaa                       >  2:208269/1‑149 (MQ=255)                                                                                                                          cAGTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGc                    <  2:1017556/151‑1 (MQ=255)                                                                                                                            gTTCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCg                  >  2:56129/1‑151 (MQ=255)                                                                                                                             ttCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGc                 >  1:971038/1‑151 (MQ=255)                                                                                                                              tCCGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGc                 <  2:796821/150‑1 (MQ=255)                                                                                                                               ccGTTCTCGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTg               >  1:95426/1‑151 (MQ=255)                                                                                                                                      cGGCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAatat         <  2:301702/150‑1 (MQ=255)                                                                                                                                        gCCCTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCAc      <  2:46981/151‑1 (MQ=255)                                                                                                                                          ccTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGc    <  1:92480/151‑1 (MQ=255)                                                                                                                                          ccTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGc    <  1:540132/151‑1 (MQ=255)                                                                                                                                          ccTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGc    <  1:1066024/151‑1 (MQ=255)                                                                                                                                           cTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCAc      <  2:328412/148‑1 (MQ=255)                                                                                                                                           cTCGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCAc      >  1:328423/1‑148 (MQ=255)                                                                                                                                             cGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCaa  <  2:652306/150‑1 (MQ=255)                                                                                                                                             cGGGCGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCaa  >  2:673263/1‑150 (MQ=255)                                                                                                                                                 |                                                                                                                                                  CGGGAGGCTGCATGAACATAGTGATCACCGGAGGGACAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAA  >  NC_000913/2421697‑2421986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑