Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,474,212	A→G	T61A (ACT→GCT)	yfdQ →	CPS‑53 (KpLE1) prophage; uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,474,212	0	A	G	100.0%	143.4 / NA	40	T61A (ACT→GCT)	yfdQ	CPS‑53 (KpLE1) prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/12);  total (28/12)

CATGCCCTTATTTCTGCTCAGGGTGTTAATGAAATTATCAGTAAGATTGGTGCCGATGCTGTGGCATTGCCTGAGAATTTCCGCATTCATGATCTGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCACTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACAC  >  NC_000913/2474071‑2474354                                                                                                                                              |                                                                                                                                               cATGCCCTTATTTCTGCTCAGGGTGTTAATGAAATTATCAGTAAGATTGGTGCCGATGCTGTGGCATTGCCTGAGAATTTCCGCATTCATGATCTGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCa                                                                                                                                       <  2:573707/151‑1 (MQ=255)  aTGCCCTTATTTCTGCTCAGGGTGTTAATGAAATTATCAGTAAGATTGGTGCCGATGCTGTGGCATTGCCTGAGAATTTCCGCATTCATGATCTGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCAt                                                                                                                                      >  2:888164/1‑151 (MQ=255)            ttCTGCTCAGGGTGTTAATGAAATTATCAGTAAGATTGGTGCCGATGCTGTGGCATTGCCTGAGAATTTCCGCATTCATGATCTGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACttt                                                                                                                            >  1:411972/1‑151 (MQ=255)                             aTGAAATTATCAGTAAGATTGGTGCCGATGCTGTGGCATTGCCTGAGAATTTCCGCATTCATGATCTGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTaa                                                                                                              >  1:483755/1‑148 (MQ=255)                                  aTTATCAGTAAGATTGGTGCCGATGCTGTGGCATTGCCTGAGAATTTCCGCATTCATGATCTGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTg                                                                                                      <  2:690071/151‑1 (MQ=255)                                     aTCAGTAAGATTGGTGCCGATGCTGTGGCATTGCCTGAGAATTTCCGCATTCATGATCTGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAg                                                                                                   <  1:560334/151‑1 (MQ=255)                                         gTAAGATTGGTGCCGATGCTGTGGCATTGCCTGAGAATTTCCGCATTCATGATCTGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATg                                                                                                >  1:798590/1‑150 (MQ=255)                                               ttGGTGCCGATGCTGTGGCATTGCCTGAGAATTTCCGCATTCATGATCTGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCAc                                                                                         >  1:946740/1‑151 (MQ=255)                                                      cGATGCTGTGGCATTGCCTGAGAATTTCCGCATTCATGATCTGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCgctgc                                                                                  <  2:203959/151‑1 (MQ=255)                                                       gATGCTGTGGCATTGCCTGAGAATTTCCGCATTCATGATCTGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCgctgct                                                                                 >  1:440975/1‑151 (MQ=255)                                                        aTGCTGTGGCATTGCCTGAGAATTTCCGCATTCATGATCTGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCtt                                                                                >  2:196085/1‑151 (MQ=255)                                                          gCTGTGGCATTGCCTGAGAATTTCCGCATTCATGATCTGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCttt                                                                               <  2:300898/150‑1 (MQ=255)                                                                 cATTGCCTGAGAATTTCCGCATTCATGATCTGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATg                                                                        >  1:938919/1‑150 (MQ=255)                                                                 cATTGCCTGAGAATTTCCGCATTCATGATCTGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGc                                                                       >  2:175002/1‑151 (MQ=255)                                                                    tGCCTGAGAATTTCCGCATTCATGATCTGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTg                                                                     <  1:490991/150‑1 (MQ=255)                                                                                  cGCATTCATGATCTGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCg                                                      >  2:451608/1‑151 (MQ=255)                                                                                  cGCATTCATGATCTGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCg                                                      <  1:773952/151‑1 (MQ=255)                                                                                     aTTCATGATCTGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTc                                                    >  2:979391/1‑150 (MQ=255)                                                                                       tCATGATCTGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAgt                                                 >  1:753632/1‑151 (MQ=255)                                                                                        cATGATCTGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAgtg                                                >  2:170896/1‑151 (MQ=255)                                                                                        cATGATCTGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAgt                                                 <  2:726485/150‑1 (MQ=255)                                                                                           gATCTGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGc                                             >  2:247074/1‑151 (MQ=255)                                                                                               tGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTa                                          >  1:456814/1‑150 (MQ=255)                                                                                                 gAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAAcc                                       >  1:130305/1‑151 (MQ=255)                                                                                                  aaaaaTTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCt                                      >  1:138276/1‑151 (MQ=255)                                                                                                      atttaatttaAATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGt                                  >  2:878733/1‑151 (MQ=255)                                                                                                          aatttaaATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACt                               >  1:794916/1‑150 (MQ=255)                                                                                                          aatttaaATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACt                               >  1:76839/1‑150 (MQ=255)                                                                                                             ttaaATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTAtt                            <  2:253181/150‑1 (MQ=255)                                                                                                             ttaaATCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATtg                           >  1:131332/1‑151 (MQ=255)                                                                                                                 aTCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGACGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATtgatg                        >  2:1031827/1‑150 (MQ=255)                                                                                                                 aTCGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATtgatga                       <  1:681894/151‑1 (MQ=255)                                                                                                                   cGCTTCCGTTTCCGTGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAAc                     >  2:671539/1‑151 (MQ=255)                                                                                                                       tccgtttccgtGGTGCGCTTCCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAg                  <  2:290398/150‑1 (MQ=255)                                                                                                                           tttccgtGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCAc             >  2:825210/1‑151 (MQ=255)                                                                                                                             tccgtGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGc           >  1:412840/1‑151 (MQ=255)                                                                                                                               cgtGGTGCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGc           >  1:803847/1‑149 (MQ=255)                                                                                                                                    tgCGCTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAac    >  1:940246/1‑151 (MQ=255)                                                                                                                                     gcgcTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAac    >  1:721478/1‑150 (MQ=255)                                                                                                                                      cgcTTTCCGCTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAacac  <  2:621400/151‑1 (MQ=255)                                                                                                                                              |                                                                                                                                               CATGCCCTTATTTCTGCTCAGGGTGTTAATGAAATTATCAGTAAGATTGGTGCCGATGCTGTGGCATTGCCTGAGAATTTCCGCATTCATGATCTGGAAAAATTTAATTTAAATCGCTTCCGTTTCCGTGGTGCGCTTTCCACTGCCAGCATCGATGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACAC  >  NC_000913/2474071‑2474354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑