Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,475,549	C→T	R13R (CGC→CGT)	yfdS →	CPS‑53 (KpLE1) prophage; uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,475,549	0	C	T	100.0%	322.6 / NA	81	R13R (CGC→CGT)	yfdS	CPS‑53 (KpLE1) prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/43);  total (38/43)

GGAAGGCATCCCGGCAACAGAGATGTTCAACGTGATTCCACTGGCACCGGGTCATGCCTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGCGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACAAAATAATACCAGTGTCAGCTGAACTGCCACCAGCAAATGAAAG  >  NC_000913/2475406‑2475695                                                                                                                                                |                                                                                                                                                   ggAAGGCATCCCGGCAACAGAGATGTTCAACGTGATTCCACTGGCACCGGGTCATGCCTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAAt                                                                                                                                             >  1:525819/1‑151 (MQ=255) ggAAGGCATCCCGGCAACAGAGATGTTCAACGTGATTCCACTGGCACCGGGTCATGCCTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAAt                                                                                                                                             <  2:760424/151‑1 (MQ=255)   aaGGCATCCCGGCAACAGAGATGTTCAACGTGATTCCACTGGCACCGGGTCATGCCTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAAtgt                                                                                                                                           >  1:222356/1‑151 (MQ=255)        aTCCCGGCAACAGAGATGTTCAACGTGATTCCACTGGCACCGGGTCATGCCTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTAcc                                                                                                                                      >  2:972594/1‑151 (MQ=255)        aTCCCGGCAACAGAGATGTTCAACGTGATTCCACTGGCACCGGGTCATGCCTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTAcc                                                                                                                                      >  2:898456/1‑151 (MQ=255)           ccGGCAACAGAGATGTTCAACGTGATTCCACTGGCACCGGGTCATGCCTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACcgcg                                                                                                                                   >  1:635158/1‑151 (MQ=255)            cGGCAACAGAGATGTTCAACGTGATTCCACTGGCACCGGGTCATGCCTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACcgcg                                                                                                                                   >  2:269351/1‑150 (MQ=255)                aaCAGAGATGTTCAACGTGATTCCACTGGCACCGGGTCATGCCTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACcgcg                                                                                                                                   >  2:723495/1‑146 (MQ=255)                      gaTGTTCAACGTGATTCCACTGGCACCGGGTCATGCCTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGaa                                                                                                                          >  1:22236/1‑149 (MQ=255)                      gaTGTTCAACGTGATTCCACTGGCACCGGGTCATGCCTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGaa                                                                                                                          >  2:289788/1‑149 (MQ=255)                         gTTCAACGTGATTCCACTGGCACCGGGTCATGCCTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTg                                                                                                                      <  1:1008231/150‑1 (MQ=255)                         gTTCAACGTGATTCCACTGGCACCGGGTCATGCCTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGa                                                                                                                     <  1:881019/151‑1 (MQ=255)                             aaCGTGATTCCACTGGCACCGGGTCATGCCTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGa                                                                                                                 <  1:349584/151‑1 (MQ=255)                                 tGATTCCACTGGCACCGGGTCATGCCTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAAc                                                                                                              >  1:320088/1‑150 (MQ=255)                                    ttCCACTGGCACCGGGTCATGCCTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGGTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACaaa                                                                                                           >  2:592486/1‑150 (MQ=255)                                      ccACTGGCACCGGGTCATGCCTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATg                                                                                                         <  2:942056/150‑1 (MQ=255)                                      ccACTGGCACCGGGTCATGCCTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGc                                                                                                        >  1:246812/1‑151 (MQ=255)                                      ccACTGGCACCGGGTCATGCCTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGc                                                                                                        <  2:335567/151‑1 (MQ=255)                                        aCTGGCACCGGGTCATGCCTACGGGATGTTTATGGAACGTTTTACCGATTTATCGGAGTTACGCAAATGCGCATTAATTTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGGAAGTGAACGAAACa                                                                                                             <  2:840520/144‑1 (MQ=255)                                        aCTGGCACCGGGTCATGCCTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTg                                                                                                      <  2:1058329/151‑1 (MQ=255)                                           ggCACCGGGTCATGCCTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGa                                                                                                     <  1:853444/149‑1 (MQ=255)                                                  ggTCATGCCTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGt                                                                                            <  1:823668/151‑1 (MQ=255)                                                     cATGCCTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTAc                                                                                          <  2:1009862/150‑1 (MQ=255)                                                     cATGCCTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACg                                                                                         >  2:1042614/1‑151 (MQ=255)                                                        gCCTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTaa                                                                                      <  1:835626/151‑1 (MQ=255)                                                        gCCTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTa                                                                                       >  2:369558/1‑150 (MQ=255)                                                         ccTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTaa                                                                                      >  1:80190/1‑150 (MQ=255)                                                          cTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAAt                                                                                    >  1:23327/1‑151 (MQ=255)                                                           tACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAAtt                                                                                   >  2:447903/1‑151 (MQ=255)                                                              gggATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCg                                                                                 >  2:325838/1‑150 (MQ=255)                                                              gggATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGa                                                                                <  1:618361/151‑1 (MQ=255)                                                               ggATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGAc                                                                               <  2:1051377/151‑1 (MQ=255)                                                                   gTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGAcgcg                                                                            <  1:384101/150‑1 (MQ=255)                                                                     ttATGGAACGTTTTAACGATTTATCGGAGTTACTCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCt                                                                          <  2:787845/150‑1 (MQ=255)                                                                     ttATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCtt                                                                         >  2:394345/1‑151 (MQ=255)                                                                       aTGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGa                                                                       <  1:687249/151‑1 (MQ=255)                                                                        tGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGa                                                                       >  2:985800/1‑150 (MQ=255)                                                                         ggAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGaa                                                                      <  1:675462/150‑1 (MQ=255)                                                                           aaCGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCt                                                                   >  1:42497/1‑151 (MQ=255)                                                                           aaCGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCt                                                                   <  2:4216/151‑1 (MQ=255)                                                                              gTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTaaa                                                                <  2:173462/151‑1 (MQ=255)                                                                                tttAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAAtg                                                              >  2:808784/1‑151 (MQ=255)                                                                                tttAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAAtg                                                              >  1:86024/1‑151 (MQ=255)                                                                                      gATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGc                                                        >  1:225290/1‑151 (MQ=255)                                                                                         ttATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCAc                                                      <  1:418629/150‑1 (MQ=255)                                                                                          tATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACt                                                     <  2:486115/150‑1 (MQ=255)                                                                                           aTCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTgg                                                   <  1:975093/151‑1 (MQ=255)                                                                                             cGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGaa                                                 <  1:752461/151‑1 (MQ=255)                                                                                             cGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGa                                                  <  2:901785/150‑1 (MQ=255)                                                                                               gAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGaaa                                                >  1:119278/1‑150 (MQ=255)                                                                                               gAGTTACGCAAACGCGCATGAATGTTTTCGAAAGGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTGCCTGGTACGTAAACTCGCCGCGCTTGCAGCTATATGGGGGGCGCttggaa                                                <  2:676883/150‑6 (MQ=255)                                                                                                 gTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACa                                             <  1:223031/151‑1 (MQ=255)                                                                                                     cGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACAAaat                                         <  2:105127/151‑1 (MQ=255)                                                                                                      gCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACAAaat                                         >  1:1005951/1‑150 (MQ=255)                                                                                                         aaTGCGCATGAATGTTTTCGGAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACAAaataat                                      >  2:259339/1‑150 (MQ=255)                                                                                                           tGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACAAAATAATAcc                                   >  2:854136/1‑151 (MQ=255)                                                                                                              gcATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACAAAATAATACCAgt                                >  1:623666/1‑151 (MQ=255)                                                                                                               cATGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACAAAATAATACCAgtg                               <  1:512681/151‑1 (MQ=255)                                                                                                                aTGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACAAAATAATACCAgtgt                              >  1:661046/1‑151 (MQ=255)                                                                                                                aTGAATGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACAAAATAATACCAgtgt                              >  1:130579/1‑151 (MQ=255)                                                                                                                    aTGTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACAAAATAATACCAGTGTCAGc                          <  2:326330/151‑1 (MQ=255)                                                                                                                      gTTTTCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACAAAATAATACCAGTGTCAGCTg                        <  1:544044/151‑1 (MQ=255)                                                                                                                         ttCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACAAAATAATACCAGTGTCAGCTGaa                      <  2:78511/150‑1 (MQ=255)                                                                                                                         ttCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACAAAATAATACCAGTGTCAGCTGaa                      <  2:652864/150‑1 (MQ=255)                                                                                                                         ttCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACAAAATAATACCAGTGTCAGCTGaa                      <  2:309048/150‑1 (MQ=255)                                                                                                                         ttCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACAAAATAATACCAGTGTCAGCTGaa                      <  1:738325/150‑1 (MQ=255)                                                                                                                          tCGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACAAAATAATACCAGTGTCAGCTGAACt                    >  1:828971/1‑151 (MQ=255)                                                                                                                           cGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACAAAATAATACCAGTGTCAGCTGAACt                    <  2:916555/150‑1 (MQ=255)                                                                                                                           cGAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACAAAATAATACCAGTGTCAGCTGAACTg                   <  2:187382/151‑1 (MQ=255)                                                                                                                            gAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACAAAATAATACCAGTGTCAGCTGAACTGc                  >  2:618796/1‑151 (MQ=255)                                                                                                                            gAAATGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACAAAATAATACCAGTGTCAGCTGAACTGc                  >  1:994220/1‑151 (MQ=255)                                                                                                                             aaaTGGAAGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACAAAATAATACCAGTGTCAGCTGAACTGcc                 >  2:831560/1‑151 (MQ=255)                                                                                                                                   aaGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACAAAATAATACCAGTGTCAGCTGAACTGCCACCAGc           >  2:446299/1‑151 (MQ=255)                                                                                                                                   aaGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACAAAATAATACCAGTGTCAGCTGAACTGCCACCAGc           >  2:750637/1‑151 (MQ=255)                                                                                                                                    aGGGTTTCTTCGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACAAAATAATACCAGTGTCAGCTGAACTGCCACCAGCa          <  1:873900/151‑1 (MQ=255)                                                                                                                                         ttcttcGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACAAAATAATACCAGTGTCAGCTGAACTGCCACCAGCAAATg      <  2:495707/150‑1 (MQ=255)                                                                                                                                         ttcttcGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACAAAATAATACCAGTGTCAGCTGAACTGCCACCAGCAAATg      <  2:650505/150‑1 (MQ=255)                                                                                                                                          tcttcGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACAAAATAATACCAGTGTCAGCTGAACTGCCACCAGCAAATGa     >  1:872341/1‑150 (MQ=255)                                                                                                                                             tcGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACAAAATAATACCAGTGTCAGCTGAACTGCCACCAGCAAATGaaa   <  1:552042/149‑1 (MQ=255)                                                                                                                                             tcGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACAAAATAATACCAGTGTCAGCTGAACTGCCACCAGCAAATGaa    <  2:229205/148‑1 (MQ=255)                                                                                                                                             tcGTGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACAAAATAATACCAGTGTCAGCTGAACTGCCACCAGCAAATGAAAg  <  2:228537/150‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                   GGAAGGCATCCCGGCAACAGAGATGTTCAACGTGATTCCACTGGCACCGGGTCATGCCTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGCGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGCTGAGTACCTGGTACGTAAATTCGACGCGCTTGAAGCTAAATGTGCGGCACTGGAAAACAAAATAATACCAGTGTCAGCTGAACTGCCACCAGCAAATGAAAG  >  NC_000913/2475406‑2475695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑