Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,640,9810CT43.6% 147.3 / 87.5 140F40F (TTC→TTT) dtpBdipeptide and tripeptide permease B
Reads supporting (aligned to +/- strand):  ref base C (13/66);  new base T (61/0);  total (74/66)
Fisher's exact test for biased strand distribution p-value = 3.60e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 147 total aligned reads displayed.

TTCAGGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTCTATGG‑CCTC‑ATTTCCATT‑GGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTTG  >  NC_000913/3640833‑3641129
                                                                                                                                                     |                                                                                                                                                           
  cAGGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCg                                                                                                                                                         <  2:1132814/151‑1 (MQ=255)
   aGGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTGt                                                                                                                                                        >  2:871031/1‑151 (MQ=255)
   aGGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGt                                                                                                                                                        <  1:1074308/151‑1 (MQ=255)
    ggATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGtt                                                                                                                                                       <  1:387863/151‑1 (MQ=255)
      aTGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTaa                                                                                                                                                     <  1:1462242/151‑1 (MQ=255)
      aTGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCCttt                                                                                                                                                      >  2:688067/1‑149 (MQ=255)
        ggTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGATTTTTTTGTTtaa                                                                                                                                                    >  2:507621/1‑150 (MQ=255)
            ttGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTGTTaaa                                                                                                                                                    >  2:356973/1‑146 (MQ=255)
             tGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTtttttttttttaaaaactt                                                                                                                                              >  2:1782609/1‑138 (MQ=255)
             tGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTtttttttctttaac                                                                                                                                                   >  1:65040/1‑138 (MQ=255)
              ggAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑g                                                                                                                                            <  1:855311/151‑1 (MQ=255)
               gAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTGTTAAAAAGCTT‑gg                                                                                                                                           >  1:1851391/1‑151 (MQ=255)
               gAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑gg                                                                                                                                           <  1:1996916/151‑1 (MQ=255)
                  ggTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTGTTAAAAAACTT‑TGagt                                                                                                                                        >  2:97270/1‑149 (MQ=255)
                      aataaataTGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTGTTTAACAGccttggtttt                                                                                                                                       >  2:725185/1‑140 (MQ=255)
                         aaataTGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTGTTTAACAACTT‑GGagt                                                                                                                                        >  2:1053897/1‑142 (MQ=255)
                          aataTGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTGTTAAACAGCTT‑GGGTTCTCGc                                                                                                                                   >  1:1987242/1‑148 (MQ=255)
                          aataTGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAag                                                                                                                                <  1:1698586/151‑1 (MQ=255)
                          aataTGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGGTTTTTTTGTTTAACAGCtttggtt                                                                                                                                         >  2:1786394/1‑138 (MQ=255)
                           atatGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTCGTTTAAAAGCCT‑GGAtttt                                                                                                                                      >  1:998333/1‑142 (MQ=255)
                           atatGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAaga                                                                                                                               <  1:1566041/151‑1 (MQ=255)
                              tGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTGTTAAAAAGCTT‑GGATTCTCGCaaaa                                                                                                                               >  2:7478/1‑146 (MQ=255)
                                aaTACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑gg                                                                                                                        <  1:1720075/151‑1 (MQ=255)
                                  tACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTGTTTAACAGCTT‑GGGTTTTCGCAAAAG‑CA‑gg                                                                                                                        >  2:261833/1‑149 (MQ=255)
                                  tACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTGTTAAAAAACTT‑TGATTTTCGCAAGAG‑CA‑gg                                                                                                                        >  1:1534710/1‑149 (MQ=255)
                                   acaacaacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCt                                                                                                                      <  2:2002837/150‑1 (MQ=255)
                                    caacaacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATTGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCt                                                                                                                      <  2:332557/149‑1 (MQ=255)
                                     aacaacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTCGTTTAACAGCTT‑GGATTTTCGCAAGAG‑CA‑GGCtttt                                                                                                                   >  1:321458/1‑151 (MQ=255)
                                     aacaacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCtttt                                                                                                                   <  2:2158181/151‑1 (MQ=255)
                                     aacaacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCtttt                                                                                                                   <  2:232476/151‑1 (MQ=255)
                                     aacaacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTCTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCtttt                                                                                                                   <  1:1522243/151‑1 (MQ=255)
                                            cccATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑Ctt                                                                                                           <  2:725447/151‑1 (MQ=255)
                                             ccATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑Cttt                                                                                                          <  2:1032505/151‑1 (MQ=255)
                                             ccATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑Cttt                                                                                                          <  2:927792/151‑1 (MQ=255)
                                              cATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑Ctttt                                                                                                         <  2:1324456/151‑1 (MQ=255)
                                              cATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑Ctttt                                                                                                         <  1:1395787/151‑1 (MQ=255)
                                               aTGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTTTTTGTTTAAAAACCTT‑GG‑TTTTCGGCAGAGCCA‑GGCTTTT‑TTA‑Ctttt                                                                                                         >  1:311454/1‑150 (MQ=255)
                                                 ggggATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGt                                                                                                      <  1:242858/151‑1 (MQ=255)
                                                 ggggATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGGTTTTTTCCTTTAAAAGCTT‑GGATTTTCGCCAGAG‑GA‑GGCTTTTTTCT‑Ctttt                                                                                                         >  2:1081719/1‑148 (MQ=255)
                                                   ggATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTGTTTAACAGCTT‑GGGTTTTCGCAAGAG‑CA‑GGCTTTTGTTA‑CTTTTtgtg                                                                                                     >  1:437054/1‑150 (MQ=255)
                                                   ggATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTCGTTTAACAGCTT‑TGGTTCTCGCCAGAG‑CA‑GGCTTTTGTCA‑CCTTTtgtg                                                                                                     >  2:449808/1‑150 (MQ=255)
                                                       gctgcAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGAACTGGC‑GGGTTTTTTTTTTAAAAAGCTT‑GGGTTTTCCCACAAG‑AA‑GGCTTTTTTTACCTTTTGGGGCttt                                                                                                 >  2:1696570/1‑151 (MQ=255)
                                                            agcaACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTGTTTAAAAGCTT‑TGGTTTTCGCAAGAG‑GA‑GGGTTTTTTCA‑CTTTTGGTGGtttt                                                                                                >  1:909522/1‑146 (MQ=255)
                                                             gcaACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTCGTTAAACAGCTT‑GGGTTCTCGCAAGAG‑CA‑GGCTTTTTTTA‑CTTTTTGTGCtttt                                                                                                >  2:165034/1‑145 (MQ=255)
                                                                 ccTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTgg                                                                                      <  2:1962426/151‑1 (MQ=255)
                                                                    cGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTc                                                                                    <  2:1943730/150‑1 (MQ=255)
                                                                      cccATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTTGTTTAAAAGCCT‑GGGTTTTCGCCAAAG‑GA‑GGCCTTTTTCC‑CTTTTTGTGCTTTTTGTGCGCTgg                                                                                      >  1:2022403/1‑146 (MQ=255)
                                                                       ccATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTGTTTAACAGCTT‑GGATTTTCGCAAGAG‑CA‑GGCTTTTTTTA‑CTTTTTGTGCTTTTTTTGCGCTGGTCTAt                                                                                 >  1:882038/1‑150 (MQ=255)
                                                                       ccATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTGTTTAAAAACTT‑GGATTTTCGCCAGAG‑CA‑GGCTTTTTTTC‑CTTTTTGTGCTTTTTCTTCGCTGGTCTAt                                                                                 >  2:756079/1‑150 (MQ=255)
                                                                          tttttCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTGTTAAAAAAATT‑GGGTTTTCGCTAGAG‑CA‑CGGTTTTTTCC‑CTTTTTGTGCTTTTTTTGCGCTgggttttt                                                                                >  2:588419/1‑142 (MQ=255)
                                                                           ttttCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGGTTTTTTTGTTTAACAACTT‑GGGTTCTTGCCAGAG‑CA‑GGCTTTTTTCA‑CTTTTTGTGTTTTTTTTGCGCTGGTTTATTG‑cc                                                                            >  2:622051/1‑150 (MQ=255)
                                                                             ttCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTCTATGG‑CCTc                                                                          <  1:1904005/150‑1 (MQ=255)
                                                                             ttCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGGTTTTTTCTTTTAAAAGCTT‑TGGTTCTTGCCAGAG‑GA‑GGGTTTTGTTA‑CTTTTTGGGGTTTTTCTGCGCTGGGCTAttggc                                                                             >  1:779970/1‑144 (MQ=255)
                                                                              tCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTCTATGG‑CCTC‑a                                                                        <  2:800165/150‑1 (MQ=255)
                                                                                aTGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTTTTAAACAGCTT‑TGGTTTTTGCCAGAG‑CA‑GGCTTTTTTTC‑CTTTTTGGGGTTTTTTTTTGCTGGGCTATGG‑CCTC‑Cttt                                                                     >  2:265885/1‑151 (MQ=255)
                                                                                 tGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTTTTAAAAAACTT‑GGGTTTTCCCCAGAG‑AAGGGCTTTTTTTA‑TTTTTGGGGTTTTTG‑TGCGCTgggttcttgccccctt                                                                        >  1:1089326/1‑135 (MQ=255)
                                                                                      ttttttGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGGTTTTTTTGTTTAAAAACTT‑TGGTTTTCGCCAGAG‑CA‑GGGTTTTTTTT‑CTTTTTGTGGTTTTTTTGCGCTGGTCTATTG‑CCTC‑Cttttcctt                                                                >  2:1589651/1‑145 (MQ=255)
                                                                                       tttttGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTCTATGG‑CCTC‑ATTTcc                                                                   <  1:1606573/146‑1 (MQ=255)
                                                                                       tttttGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTCTATGG‑CCTC‑ATTTCCAtt                                                                <  1:166854/149‑1 (MQ=255)
                                                                                           tGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTCTATGG‑CCTC‑ATTTCCATT‑ggcgg                                                          <  1:1417737/150‑1 (MQ=255)
                                                                                              cGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTGTTTAAAAGCTT‑GGATTTTCGCCAAAG‑CA‑GGCTTTTTTCC‑CTTTTTGTGCTTTTTCTGCGCTTGGCTATGG‑CCTC‑CTTTTCCTT‑tgcgggtct                                                      >  2:895097/1‑147 (MQ=255)
                                                                                              cGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTCTATGG‑CCTC‑ATTTCCATT‑GGCGGCTAt                                                      <  2:1882864/151‑1 (MQ=255)
                                                                                              cGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGGTTTTTTTGTTTAAAAACTT‑GGGTTTTCGCAAGAG‑CA‑GGCTTTTTTCA‑CTTTTTGTGCTTTTGCTGCGCTGGTCTATTG‑CCTC‑ATTTTCCTT‑GGCGGGTAt                                                      >  1:2071862/1‑151 (MQ=255)
                                                                                                aGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTCTATGG‑CCTC‑ATTTCCATT‑GGCGGCTATGt                                                    <  1:1864853/151‑1 (MQ=255)
                                                                                                 gTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTTAACAGCTT‑GGGATCTTGCAACAG‑CA‑GGCCTTTTTCA‑CTTTTTGTGGCTTTTTTTCGCTGGGCTATGGCCCTC‑TTTTccctggcgggcttg                                                       >  1:1002491/1‑137 (MQ=255)
                                                                                                  ttATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTGTTAAAAAGCTT‑TGGTTCTCGCAAAAG‑CA‑GGGTTTTTTCA‑CTTTTTGTGCTTTTTCTGCGCTGGTCTATGG‑GCTC‑ATTTTCCTT‑TGCGGCttt                                                      >  1:82278/1‑145 (MQ=255)
                                                                                                   tATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTCTTTAAAAGCTT‑GGATTTTCCCAAGAG‑CA‑AGCTTTTTTTT‑CATTTTGTGCTTTTGCTTCGCTGGTTTTTCG‑CCCC‑TTTTCCATTGGGCGGCtttttt                                                   >  2:80702/1‑145 (MQ=255)
                                                                                                   tATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTCGTTAAAAAGCTT‑TGGTTCTCGCAAGAG‑CA‑GGCTTTTGTTT‑CTTTTTGTGCTTTTTCTTCGCTGGTCTATGG‑TCTC‑ATTTTCATT‑TGCGGCtcttt                                                    >  2:1220127/1‑144 (MQ=255)
                                                                                                   tATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTCTATGG‑CCTC‑ATTTCCATT‑GGCGGCTATGTCg                                                  <  2:131668/150‑1 (MQ=255)
                                                                                                   tATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTCTATGG‑CCTC‑ATTTCCATT‑GGCGGCTATGTCg                                                  <  2:847845/150‑1 (MQ=255)
                                                                                                    aTGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTCTTTTAACAACCT‑TGGTTTTCGCCAGAG‑CA‑AGGTTTTTTTA‑CTTTTTGTGCTTTTTCTGCGCCGGGCTTTGG‑CCTC‑CTTTTCCTT‑TGGGGCtct                                                      >  2:1481415/1‑143 (MQ=255)
                                                                                                    aTGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTCGTTTAACAGCTT‑GGGTTTTCGCAAAAG‑CA‑GGCTTTTTTTA‑CTTTTTGTGCTTTTTCTTCGCTGGTCTATTG‑GCTC‑ATTTTCCTT‑TGCGGCTATTTCgg                                                 >  1:1887420/1‑150 (MQ=255)
                                                                                                      gggAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTCTATGG‑CCTC‑ATTTCCATT‑GGCGGCTATGTCGGCg                                               <  2:633180/150‑1 (MQ=255)
                                                                                                        gAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTCTATGG‑CCTC‑ATTTCCATT‑GGCGGCTATGTCGGCGacc                                            <  1:1782641/151‑1 (MQ=255)
                                                                                                         aGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTGTTAAACAGCCT‑TGGTTTTCGCAAGAG‑GA‑GGCTTTTTTCA‑CTTTTTGTGCTTTTTCTTCGCTTGTCTTTTG‑CCTC‑ATTTTCATT‑tgcgggttttttggcggcc                                            >  1:1628031/1‑136 (MQ=255)
                                                                                                            gATTCGGCTACTACGGCATGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTCTATGG‑CCTC‑ATTTCCATT‑GGCGGCTATGTCGGCGACCACct                                        <  2:1849952/151‑1 (MQ=255)
                                                                                                             aTTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTTGTTAAAAAGCTT‑GGGTTTTCGCAAAAG‑CA‑GGGTTTTGTCA‑CTTTTTGGGGTTTTTCTGCGCTGGTCTATGG‑CCTC‑ATTTTCATT‑TGCGGGTATGTTGGCGaccacc                                         >  1:593094/1‑149 (MQ=255)
                                                                                                                cGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTTTTTTAAAAGCTT‑GGGTTTTCGCAAGAG‑CA‑GGCCTTTGTTA‑ATTTTTGGGGTTTTTGTGCGGTGGGCTATGG‑GCCC‑ATTTTCATT‑TGCGGGTTTGTTGGGGagcccc                                         >  2:666034/1‑141 (MQ=255)
                                                                                                                cGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTCTATGG‑CCTC‑ATTTCCATT‑GGCGGCTATGTCGGCGACCACctgct                                     <  2:941779/150‑1 (MQ=255)
                                                                                                                  gCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTTAAAAACCT‑GGGTTTTCGCCAGAG‑CA‑GGCCTTTTTCA‑CTTTTTGTGCTTTTTCTTCGCTGGTCTTTGG‑CCTC‑CTTTCCCTT‑TGCGGCTTTTTTGGGGGCCACCCGCTggg                                  >  1:1458140/1‑151 (MQ=255)
                                                                                                                  gCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTCTATGG‑CCTC‑ATTTCCATT‑GGCGGCTATGTCGGCGACCACCTGCTggg                                  <  1:1776695/151‑1 (MQ=255)
                                                                                                                    tactacGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTCGTTAAACAGCTT‑GGATTTTCGCAAGAG‑CA‑GGCTTTTGTTA‑CTTTTTGGGGTTTTTGTGCGCTGGTCTATTG‑CCCC‑ATTTTCATT‑GGGGGCTATGTTGGGGACCCCCTGCTgggg                                 >  1:522370/1‑150 (MQ=255)
                                                                                                                     actacGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTCGTTTAAAAGCTTGGGGTTCTGGC‑AGAG‑CA‑GGGTTTTTTTA‑CTTTTTGGGCTTTTTCTTCGCTGGCCTATGG‑CCCCAATTTCCTTT‑GG‑GGCTATTTTGGCCGCCACCCGGTggggg                                >  1:610982/1‑149 (MQ=255)
                                                                                                                     actacGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCCGCGCTGGTCTATGG‑CCTC‑ATTTCCATT‑GGCGGCTATGTCGGCGACCACCTGCTGGGGa                                <  2:1858287/150‑1 (MQ=255)
                                                                                                                        acGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTCTATGG‑CCTC‑ATTTCCATT‑GGCGGCTATGTCGGCGACCACCTGCTGGGGACCa                             <  1:2086133/150‑1 (MQ=255)
                                                                                                                              tGCAGGGCGTACTGGC‑GGGTTTTTTCGTTAAACAACTT‑GGATTCTCGCAAGAG‑CA‑GGGTTTTGTTA‑CTTTTTGGGCTTTTTCTGCGCTGGTCTATGG‑CCCC‑CTTTTCATT‑GGCGGCTTTGTTGGCGgccaccctccggggggccaa                           >  2:1382760/1‑132 (MQ=255)
                                                                                                                                cAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTCTATGG‑CCTC‑ATTTCCATT‑GGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCa                    <  1:1324567/151‑1 (MQ=255)
                                                                                                                                  gggCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTCTATGG‑CCTC‑ATTTCCATT‑GGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCAtt                  <  1:986371/151‑1 (MQ=255)
                                                                                                                                     cGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTCTATGG‑CCTC‑ATTTCCATT‑GGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGtt               <  2:1664960/151‑1 (MQ=255)
                                                                                                                                     cGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTCTATGG‑CCTC‑ATTTCCATT‑GGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGtt               <  2:1822276/151‑1 (MQ=255)
                                                                                                                                        aCTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTCTATGG‑CCTC‑ATTTCCATT‑GGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCtt            <  1:430272/151‑1 (MQ=255)
                                                                                                                                         cTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTCTATGG‑CCTC‑ATTTCCATT‑GGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCtt            >  2:1020345/1‑150 (MQ=255)
                                                                                                                                          tGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTCTATGG‑CCTC‑ATTTCCATT‑GGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTgg          >  2:2127070/1‑151 (MQ=255)
                                                                                                                                           ggC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTCTATGG‑CCTC‑ATTTCCATT‑GGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTgg          <  1:586449/150‑1 (MQ=255)
                                                                                                                                           ggC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTCTATGG‑CCTC‑ATTTCCATT‑GGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTg           <  1:1331318/149‑1 (MQ=255)
                                                                                                                                           ggC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTCTATGG‑CCTC‑ATTTCCATT‑GGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTg           <  1:669940/149‑1 (MQ=255)
                                                                                                                                            gC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTCTATGG‑CCTC‑ATTTCCATT‑GGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTgg          >  1:1560354/1‑149 (MQ=255)
                                                                                                                                            gC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTCTATGG‑CCTC‑ATTTCCATT‑GGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAg        <  2:1159606/151‑1 (MQ=255)
                                                                                                                                             c‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTCTATGG‑CCTC‑ATTTCCATT‑GGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGc       <  1:871043/151‑1 (MQ=255)
                                                                                                                                                 ttttCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTCTATGG‑CCTC‑ATTTCCATT‑GGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACt    >  2:1412388/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                           
TTCAGGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTT‑GGATTCTCGCAAGAG‑CA‑GGCTTTTGTCA‑CTTTTGGTGCTTTTGCTGCGCTGGTCTATGG‑CCTC‑ATTTCCATT‑GGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTTG  >  NC_000913/3640833‑3641129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑