Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913436,5160CA42.7% 190.7 / 55.6 110Q295K (CAA→AAA) thiLthiamine monophosphate kinase
Reads supporting (aligned to +/- strand):  ref base C (50/13);  new base A (0/47);  total (50/60)
Fisher's exact test for biased strand distribution p-value = 1.84e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 112 total aligned reads displayed.

CTTTCTCGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTC  >  NC_000913/436366‑436666
                                                                                                                                                       |                                                                                                                                                       
cTTTCTCGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑c                                                                                                                                                         >  2:2052840/1‑151 (MQ=255)
  ttCTCGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑Ca                                                                                                                                                        >  2:1676835/1‑150 (MQ=255)
  ttCTCGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑Ca                                                                                                                                                        >  1:239947/1‑150 (MQ=255)
   tctcGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑Caa                                                                                                                                                       >  2:858268/1‑150 (MQ=255)
   tctcGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑Caa                                                                                                                                                       >  2:313285/1‑150 (MQ=255)
   tctcGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑Caa                                                                                                                                                       >  2:432878/1‑150 (MQ=255)
   tctcGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑Caa                                                                                                                                                       >  2:2118827/1‑150 (MQ=255)
     tcGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑Tg                                                                                                                                                   >  1:525115/1‑151 (MQ=255)
     tcGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑Tg                                                                                                                                                   >  1:96291/1‑151 (MQ=255)
      cGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑Caaa                                                                                                                                                      <  2:403888/148‑1 (MQ=255)
      cGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGa                                                                                                                                                  <  1:477278/151‑1 (MQ=255)
       gCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGAc                                                                                                                                                 <  1:1821766/151‑1 (MQ=255)
        ccATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGAcc                                                                                                                                                <  2:1572733/151‑1 (MQ=255)
          aTGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGAccg                                                                                                                                               <  2:1534932/150‑1 (MQ=255)
           tGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGAccgcc                                                                                                                                             >  1:721702/1‑151 (MQ=255)
               gAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGACCGCCGat                                                                                                                                          >  2:1906387/1‑150 (MQ=255)
                aacgggaacgggCGCGGCGGTGGGCGCTCTGGGGGGGTGAAGATTACGAGTTGTTTTTCACTGTGCCGGAACTGAACCGTGGCGCGCGGGATGTGGCTCCCGGACACCGGGGCGTACCGTTTCCTTGTATCGGG‑CAAA‑TGACCGCCGata                                                                                                                                         <  2:278083/150‑1 (MQ=255)
                 aCCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGACCGCCGATATc                                                                                                                                       >  1:30235/1‑151 (MQ=255)
                      aaCAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGACCGCCGATATCGAAgg                                                                                                                                  >  2:1054138/1‑151 (MQ=255)
                           gcgctgcgctGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCttt                                                                                                                             >  1:855504/1‑151 (MQ=255)
                               tgcgctgGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGttt                                                                                                                         >  1:400396/1‑151 (MQ=255)
                               tgcgctgGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGttt                                                                                                                         >  1:1089626/1‑151 (MQ=255)
                               tgcgctgGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGttt                                                                                                                         >  1:233562/1‑151 (MQ=255)
                                gcggtgGGCGCTCTCTGGGGGTAAAGATTAGGAGTTGTTTTTCATTGGGCCGGAACTGAACCTTGGCGCGCTGGTTGTGGCTCTCGGCCACCGGGGCGTACCGTTTCCCTGTATCGGG‑AAAA‑GGACCGCCGATATCGAAGGGCTTTGt                                                                                                                           <  1:1426869/148‑1 (MQ=255)
                                gcgctgGGCGCTCTCGGGCGGTGAAGATTACGAGTTGTTTTTCACTGTGCCGGAACTGACCCGTGGCGCGTTGGTTGTGGCTCTGGGACACCGGGGCGTCCCGTTTCCCTGTATGGGG‑AAAA‑TGACCGCCGATATCGAAGGGCTTTGtt                                                                                                                          <  1:1229220/149‑1 (MQ=255)
                                  gctgGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTAt                                                                                                                      >  1:1324220/1‑151 (MQ=255)
                                     gggCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCg                                                                                                                   >  2:2059652/1‑151 (MQ=255)
                                     gggCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCg                                                                                                                   >  2:790940/1‑151 (MQ=255)
                                     gggCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCg                                                                                                                   >  2:81730/1‑151 (MQ=255)
                                     gggCGCTCTCGGGGGGTGAAGATTACGGTTTTTTTTTCCCTGGGCCGGAACTGACCCGTGGCGCGTTGGTTGGGGCTCTCGGACACGGGGGCGTCCCTTTTCCTTGTATGGGG‑AAAA‑TGACCGCCGATATCGAAGGGCttt                                                                                                                             <  1:1485303/141‑1 (MQ=255)
                                     gggCGCTCTCGGGCGGTGAAGTTTACGTTTTGTGTTTCACTGTCCGGGAACTGACCCGGGGCGCGCTGGATGTGGCTCTCGGACCCCGGGGCGTCCCTTTTCCCTGTAGCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGtttt                                                                                                                        <  2:123016/146‑1 (MQ=255)
                                     gggCGCTCTCGGGCGGTAAAGATTACGTGTTGTTTTTCACTGTGCCGGAACTAACCCGGGGCGCGCTGGTTGTGGCTCTCGCACACCGGGGCGTACTTTTTCCCTGTATGGGA‑AAAA‑GGACCGCCGATATCGAAGGGCTTTGTTTTAt                                                                                                                      <  2:1955528/148‑1 (MQ=255)
                                     gggCGCTCTCGGGCGGGGAAGTTTACGGGTTTTTTTTCCCTGTGCCGGAACTGACCCGGGGCGCGTTGGTGGGGGCTCCCGGACACCTGGGCGTCCCTTTTCCTTTTATGGGG‑AAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTc                                                                                                                    <  2:2029834/150‑1 (MQ=255)
                                        cgcTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTg                                                                                                                 >  2:1964481/1‑150 (MQ=255)
                                           tctctGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACgg                                                                                                             >  1:1612368/1‑151 (MQ=255)
                                           tctcgGGCGGTGAATTTTACGTGTTTTTTTTCACTGCGCCGGAATTGCCCCGGGGCGCGTTGAATGTGCCTCCCGGACCCCTGGGCGTCCTTTTTCCTTGAAGGGGG‑AAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTAt                                                                                                                      <  2:1311182/142‑1 (MQ=255)
                                               tGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGa                                                                                                          >  1:1612830/1‑150 (MQ=255)
                                                gggggggAAGATTACGATTGTTTTTTCCCTGTGCGGAAACTGACCCGGGGCGGGCGGGTTGTGGCTCTGGAACACCGGGGTGTCCCTTTTCCCTTTACGGGG‑AAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACg                                                                                                              <  2:480270/139‑1 (MQ=255)
                                                gggggTGAAGATTAGGGGTTGTTTTTCCGTGTGCGGAAACTGACCGGGGGCGGGTGGGATGGGGCTCCCGGCCACCTGGGGTTCCTTTTTCCCTGTATGGGG‑AAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGaa                                                                                                         <  2:42779/147‑1 (MQ=255)
                                                gggggTGAAGATTACGGTTTGTGTTTCCCTGTGCCGAACCTGACCGGTGGCGCGGTGGATGTGGCTCTCGGCCACCGGGGCGTCCGTTTTCCCTGTATCGGG‑AAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGc                                                                                                            <  2:1279230/144‑1 (MQ=255)
                                                ggCGGTGAAGATTACGAGTTGTTTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCTTTTACTTGTATCGGG‑AAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAAc                                                                                                        <  1:1918311/151‑1 (MQ=255)
                                                ggCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAAc                                                                                                        >  1:1274400/1‑151 (MQ=255)
                                                ggCGGTAAAGTTTACGTTTTTTGTTTCACTGTCCGGGAACTAACCCGGGGCGCGCTGGATGTGGCTCTCGGACCCGTGGGCTTCCCTTTTCCCTGTATCGGG‑AAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGc                                                                                                            <  2:1702846/147‑1 (MQ=255)
                                                ggCGGGAAAGATTAGGGGTTGTTTTTCCCTGTGCCGGAACGGAACCGGGGCGCGTTGGATGTGGTTCTGGGCCACCTGGGCGTACCTTTTCCTTTTATGGGG‑AAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGAc                                                                                                               <  2:272163/144‑1 (MQ=255)
                                                   ggTGAAGATTACGAGTTGTGTTTCCCTGTGCCGGAACTGAACCGGGGCGCGCTGGATGTGGCTCTCGGACCCCTGGGCGTCCCGTTTCCCTGTAGCGGG‑AAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAAcc                                                                                                       <  1:1021541/149‑1 (MQ=255)
                                                   gcggagaatatggggTTGTGTTTCCCGGCGACGGCACTGAGCGGTGGGGCGCTGGGGGTGGTTCTCGAACCCTTTGGCGTCCCGTGTCCATTTATGGGG‑AAAA‑TTTACGCCGATATCGACGGGCTTTGTTTTATTCGTGACGGCGAACCt                                                                                                      <  2:556355/136‑1 (MQ=255)
                                                     tGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGTCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGtt                                                                                                   >  2:1410799/1‑151 (MQ=255)
                                                       aaGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTAc                                                                                                 >  2:2096305/1‑151 (MQ=255)
                                                         gATTACGAGTTGTTTTTCACTGTGCCGGAACTGACCCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGTGTCCCGTTTACCTGTAGCGGA‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACa                                                                                                <  2:1919063/150‑1 (MQ=255)
                                                           ttACGGTTTGTTTTTCCCTGTGCGGAAACTGACCGGTGGCGCGGTGGATGTGGCTCTCGGACCCCTGGGCGTCCCTTTTCCCTGTATGGGG‑AAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACAtt                                                                                              <  1:1994578/150‑1 (MQ=255)
                                                           ttACGGGTTGTTTTTCACTGTGCCGGAACTGACCCGTGGCGCGTTGGATGTGGCTCTCGGACCCCGGGGCGTCCCTTTTCCTTTTACGGGG‑AAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACAtt                                                                                              <  1:2052884/150‑1 (MQ=255)
                                                            tacgttttGTTTTTCACTGTGCGGGACCTAACCGGTGGCGCGCTGGATGTGGCTCTCGAACCCCTGGGCTTCCTTTTTCCTTGTTTGGGG‑AAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTa                                                                                             <  2:1394723/144‑1 (MQ=255)
                                                               gAGTTGTTTTTCACTGTGCCGGAACTGACCGGGGGCGCGCTGGATGTGGCTCTCGGACACCGGGGCGTCCCGTTTACCTTTATCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGa                                                                                           <  1:115451/149‑1 (MQ=255)
                                                                 gTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTgg                                                                                       >  2:1391449/1‑151 (MQ=255)
                                                                  ttttttttCCCTGTGCCGAACCTGACCCGTGGCGCGCTGGATGTGGCTCTCGGACCCCTGGGCTTCCCTTTTCCCTGTACCGGG‑AAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGAc                                                                                          <  2:198258/142‑1 (MQ=255)
                                                                      gtTTCACTGTGCCGAAACTGACCCGGGGCGCGCTGGATGTGGCTCTCGGCCCCCGGGGCGTCCCGTTTCCTTGTATGGGG‑AAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAg                                                                                   <  1:1032247/150‑1 (MQ=255)
                                                                        ttCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGat                                                                                >  1:1221310/1‑151 (MQ=255)
                                                                         tcccTGTGCCGGACCTGACCCGGGGGGCGGTGGATGTGGCTCTCGGCCCCCTGGGCGTCCTTTTTCCCTGTTTCGGG‑AAAA‑AGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGat                                                                                <  2:1386486/147‑1 (MQ=255)
                                                                         tCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGat                                                                                >  1:880267/1‑150 (MQ=255)
                                                                          cccTGGGCGGGAACTGAACCGTGGCGCGCTGGATGGGGCTCTCGGACCCGTGGGCGTCCCGTTTCCCTGTATGGGG‑AAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGatat                                                                              <  1:1605327/149‑1 (MQ=255)
                                                                                  cggatctaaccggTGGCGCTTTGGTTTTGGCTCCCGAACCCCGGGGCTTCCCTTTTCCCTTTATGGGG‑CAAA‑TAACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCAtt                                                                       <  1:510892/138‑1 (MQ=255)
                                                                                     accTGACCCGGGGCGCGTTGGATGTGGCTCTCGGCCACTGGGGCTTACCTTTTCCTTGTATCGGG‑AAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTg                                                                    <  2:760213/148‑1 (MQ=255)
                                                                                     aaCTGACCGGGGGGGCGGTGGTTGTGCCTCTCGGACACCGGGGCGTCCTGTTTCCCTGTATGGGG‑AAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCAtttt                                                                     <  2:541741/149‑1 (MQ=255)
                                                                                      aCTAAACCGGGGCGCGTGGGTGGTGTCTCTCGAACCCGTGGGCGTCCCTTTTCCCTGTAGGGGG‑AAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCAttt                                                                      <  2:2128796/147‑1 (MQ=255)
                                                                                        tGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCAc                                                                >  1:1559021/1‑151 (MQ=255)
                                                                                         gacccGTGGCGCGCTGGTTGGGGCTCTGGGCCACCGGGGCGTCCTTTTTTCTTGTTTCGGG‑AAAA‑TTACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACg                                                               <  1:1277498/148‑1 (MQ=255)
                                                                                         gAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACg                                                               >  2:1505176/1‑151 (MQ=255)
                                                                                          accgggggCGCGTTGGATGTGGCTCTCGGACCCCGGGGCTTCCCTTTTCCCTGTTTGGGG‑AAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCAc                                                                <  2:801126/143‑1 (MQ=255)
                                                                                          acccGTGGGGCGCTGGATGTGGCTCTCGGACACCGGGGCGTCCCTTTTCCTTGTATGGGG‑AAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACg                                                               <  1:26778/148‑1 (MQ=255)
                                                                                                ggCGGGTTGGGTGGGGCTCGCGGACCCTGGGGCGTCCTGTTTTTCTCTATCGGG‑CAAA‑TGTCCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGcc                                                                  <  1:1178849/141‑1 (MQ=255)
                                                                                                ggCGCGCTGGATGTGGCTCTCGGCCCCCTGGGCTTCCCTTTTCCCTTTGCCGGA‑AAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATaa                                                         <  2:694481/150‑1 (MQ=255)
                                                                                                  cgcgcTGGATGTGGCTCTCGGCCACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATaa                                                         >  1:2109776/1‑148 (MQ=255)
                                                                                                    cgcTGGTTGTGGCTCTGGGACCCCTGGGCGTCCCTTTTCCTTTTAGCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGa                                                      <  1:1329097/149‑1 (MQ=255)
                                                                                                     gtgggAGGTGCCTCTCGGACCCCGGGGCGTCCCTTTTACCTGTACGGGG‑AAAA‑GGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATg                                                    <  2:1327179/147‑1 (MQ=255)
                                                                                                     gcTGGATGTGCCTCGGGGCCCCGTGGGGGTACCTTTTCCCTTTATCGGG‑AAAA‑TGTCCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGAt                                                     <  1:1702815/149‑1 (MQ=255)
                                                                                                         gATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTcgcg                                               >  2:2109942/1‑151 (MQ=255)
                                                                                                           tgtgGCCCCCGGACACCCGGGCGCACCGTTTACCTGTATCGGG‑CAAA‑TGACCGCCGATCTCGACGGGCTTTGTTTTATTCGTGACGGCGAACCTGTCACATTCGACTGGAAAGGATATGACCATTTCGCCACGCCATACACATgcccc                                                >  2:1540419/1‑144 (MQ=255)
                                                                                                              ggCTCTCGGCCCCGGGGGCG‑ACCGTTTTCCTTTTACGGGAAAAA‑TAACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGaa                                             <  2:1234252/148‑1 (MQ=255)
                                                                                                                     ggcccctggggCGTCCCTTTTCCCTGTATGGGG‑AAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGaaaa                                           <  1:1363748/135‑1 (MQ=255)
                                                                                                                      ggccccgggggcttccGTTTCCCTTTTCCGGG‑AAAATTGCCCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGaa                                             <  2:886092/127‑1 (MQ=255)
                                                                                                                       acacCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGa                                  >  1:882525/1‑150 (MQ=255)
                                                                                                                        ccccggggCGTACCTTTTCCCTGTAGCGGG‑AAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAg                                <  1:407994/146‑1 (MQ=255)
                                                                                                                           cTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATg                             >  1:2014516/1‑151 (MQ=255)
                                                                                                                             gggtgtcctttttACTTGTATGGGA‑AAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGaa                                 <  1:777667/135‑1 (MQ=255)
                                                                                                                             gggCGTACCGTTTACCTGTATCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATg                             >  1:495308/1‑149 (MQ=255)
                                                                                                                                 gTACCGTTTCCCGGTTTCGGA‑AAAT‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTaa                        <  1:786508/150‑1 (MQ=255)
                                                                                                                                    ccGTTTACCTGTATCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCg                    >  2:820340/1‑151 (MQ=255)
                                                                                                                                      ttttcctggttggggg‑AAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATcc                     <  1:1053219/135‑1 (MQ=255)
                                                                                                                                       tttCCCTGTATGGGG‑AAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTg                  <  1:708444/150‑1 (MQ=255)
                                                                                                                                        ttccttGTATGGGA‑AAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCAGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTgg                 <  2:593079/145‑1 (MQ=255)
                                                                                                                                          aCCTGTATCGGG‑CAAA‑TGACCGCCGTTATCGAATGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTAGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCAt              >  2:67746/1‑151 (MQ=255)
                                                                                                                                           ccTGTATCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATc             >  1:1192374/1‑151 (MQ=255)
                                                                                                                                             tGTATCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCt            >  1:281944/1‑150 (MQ=255)
                                                                                                                                                aTCGGG‑AAAA‑TGCCCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTa           <  2:326830/148‑1 (MQ=255)
                                                                                                                                                 tCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTg       >  1:2094810/1‑151 (MQ=255)
                                                                                                                                                  cGGG‑AAAA‑TGGCCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTg       <  1:1748731/150‑1 (MQ=255)
                                                                                                                                                   ggg‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCt     >  2:1619921/1‑151 (MQ=255)
                                                                                                                                                   ggg‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCt     >  2:871022/1‑151 (MQ=255)
                                                                                                                                                   ggg‑AAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACtt        <  2:1065829/148‑1 (MQ=255)
                                                                                                                                                       cAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTc  >  2:1635315/1‑151 (MQ=255)
                                                                                                                                                       |                                                                                                                                                       
CTTTCTCGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGG‑CAAA‑TGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTC  >  NC_000913/436366‑436666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑