Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913599,5760TG56.7% 107.4 / 201.0 127V288G (GTG→GGG) cusAcopper/silver efflux system, membrane component
Reads supporting (aligned to +/- strand):  ref base T (6/49);  new base G (72/0);  total (78/49)
Fisher's exact test for biased strand distribution p-value = 6.63e-29
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.97e-19

Only 100 of 138 total aligned reads displayed.

AACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACCT  >  NC_000913/599427‑599726
                                                                                                                                                      |                                                                                                                                                       
aaCGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGggtg                                                                                                                                                        <  2:1510166/151‑1 (MQ=255)
     tCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGgggggggg                                                                                                                                                     >  2:248402/1‑147 (MQ=255)
      cgacgaCTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGgggggggggt                                                                                                                                                   >  2:1406346/1‑146 (MQ=255)
      cgacgaCTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGggggggggg                                                                                                                                                    >  1:825711/1‑146 (MQ=255)
       gacgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGgggggggggtc                                                                                                                                                  >  2:1129571/1‑145 (MQ=255)
       gacgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGgggggggggt                                                                                                                                                   >  2:174272/1‑145 (MQ=255)
        acgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑Ggggggggggggggttc                                                                                                                                                 >  2:199264/1‑144 (MQ=255)
        acgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGggggggggggcc                                                                                                                                                 >  2:1224337/1‑144 (MQ=255)
         cgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGGG‑Tcc                                                                                                                                                >  1:1903775/1‑149 (MQ=255)
          gacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGgggggggg                                                                                                                                                     >  2:1782236/1‑142 (MQ=255)
          gacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGc                                                                                                                                             <  2:1796214/151‑1 (MQ=255)
           acTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGGA‑Gcc                                                                                                                                                >  2:1292537/1‑147 (MQ=255)
            cTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGGA‑TCCTGc                                                                                                                                             >  1:1243844/1‑149 (MQ=255)
              ttAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGTGG‑Tccccc                                                                                                                                             >  2:790169/1‑144 (MQ=255)
               tAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATc                                                                                                                                         <  1:94774/150‑1 (MQ=255)
                   cacaTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGGG‑TCCTGCGGTACgg                                                                                                                                      >  2:142037/1‑149 (MQ=255)
                   cacaTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGG‑TCCTGCGATCCGGc                                                                                                                                     >  2:2171458/1‑150 (MQ=255)
                    acaTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCaa                                                                                                                                   <  1:1597484/151‑1 (MQ=255)
                      aTCGTTTTAACAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGTGA‑GCCTGGGATCCGGGaaa                                                                                                                                  >  1:313221/1‑150 (MQ=255)
                      aTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGG‑GCCTTCGGTCCCGCaaa                                                                                                                                  >  2:1918514/1‑150 (MQ=255)
                      aTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGA‑TCCTGCGATCCGGCaa                                                                                                                                   >  1:1617387/1‑149 (MQ=255)
                       tCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGG‑TCCTGCGATCCGGCaaaa                                                                                                                                 >  1:833640/1‑150 (MQ=255)
                        cGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCaaaaa                                                                                                                                <  2:1824796/150‑1 (MQ=255)
                         gTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGGG‑TCCTGCGAAACGGCaaa                                                                                                                                  >  2:1381085/1‑147 (MQ=255)
                         gTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGG‑TCCTGCGGTCCGGCcaaaa                                                                                                                                >  2:1428782/1‑149 (MQ=255)
                           tttAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGGG‑TCCTGCGGTCCGGCCAAAAAGcc                                                                                                                            >  2:286595/1‑151 (MQ=255)
                              aaaaGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCga                                                                                                                         <  2:276085/151‑1 (MQ=255)
                              aaaaGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGG‑GCCTGCGCTCCCGCCAAAAAGccc                                                                                                                           >  2:352864/1‑149 (MQ=255)
                                 aGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGG‑TCCTGCGATCCGGGAAAAAAGccc                                                                                                                           >  1:378407/1‑146 (MQ=255)
                                   ccAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGGACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGG‑TGCTTCGGGCCCGCAAAAACGcccccgcag                                                                                                                     >  1:240649/1‑144 (MQ=255)
                                   ccAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGGG‑TCCTGCGATCCGGCCAAAAAGCCCGCGaa                                                                                                                      >  2:1929270/1‑149 (MQ=255)
                                     aGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGa                                                                                                                  <  2:61220/151‑1 (MQ=255)
                                     aGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGG‑TCCTGCGATCCGGCAAAAACGCCCcagga                                                                                                                      >  2:1574049/1‑145 (MQ=255)
                                        gAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGGA‑TACTGCGATTCGGCAAAAAAGCCCGAGGAGGGATc                                                                                                                >  2:942798/1‑150 (MQ=255)
                                         aaaaTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATcgc                                                                                                              <  2:555092/151‑1 (MQ=255)
                                          aaaTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGG‑TCCTGCGGTCCGGCAAAAAAGCCCGGGGAGGGATcg                                                                                                               >  2:1426289/1‑149 (MQ=255)
                                          aaaTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGG‑TCCTGCGATCCGGCAAAAAAGCCCGAGGAGTGATcgcc                                                                                                             >  2:1120650/1‑151 (MQ=255)
                                           aaTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGA‑TCCGCCGTCCCGGCAAAAAACCCCggaaagggttccc                                                                                                              >  1:643151/1‑138 (MQ=255)
                                           aaTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGTGG‑GCCTTGGATCCGGCAAAAAAGCCCGAGAAGGGATc                                                                                                                >  1:1838598/1‑147 (MQ=255)
                                             tGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGA‑TCCTGCGATCCCGCAAAAACGCCCGGGAAGGGATAgccgcc                                                                                                          >  2:283056/1‑151 (MQ=255)
                                              ggCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGccgccg                                                                                                         <  1:2152065/151‑1 (MQ=255)
                                                 gTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGGTCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGa                                                                                                      <  2:843799/151‑1 (MQ=255)
                                                 gTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTg                                                                                                       >  1:813253/1‑150 (MQ=255)
                                                 gTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGG‑TCCCGCGGTCCCGCAAAAAAGCCCGAGAAGGGATCgccgcc                                                                                                          >  2:795487/1‑147 (MQ=255)
                                                   gCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAg                                                                                                    <  1:1147676/151‑1 (MQ=255)
                                                    cccGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAgg                                                                                                   <  1:1820715/151‑1 (MQ=255)
                                                    cccGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAgg                                                                                                   <  1:1656265/151‑1 (MQ=255)
                                                    cccGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAgg                                                                                                   >  1:1423183/1‑151 (MQ=255)
                                                       gTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGa                                                                                                  >  1:2088918/1‑149 (MQ=255)
                                                        tttATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGGGA‑GCCTGCGATCCGGCAAAAACGCCCCAGAAGTGGTCGCCGGCGGGAAgg                                                                                                   >  2:1004507/1‑147 (MQ=255)
                                                          tATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACaaa                                                                                              <  1:1238477/150‑1 (MQ=255)
                                                           aTCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACaaa                                                                                              >  1:692784/1‑149 (MQ=255)
                                                           aTCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGG‑TCCTGCGATCCGGCAAAAAAGCCCGAGAAGGGATCGCCGCCGTGAAGGACAAAc                                                                                             >  1:2039329/1‑150 (MQ=255)
                                                             cTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGGATTCCCCGGGTCCGG‑AAAAAAGCCCGGGAAGTGGTAgacgcccggcgtggcacac                                                                                             >  2:2109269/1‑135 (MQ=255)
                                                              tGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGG‑TCCTGCGGACCCGCAAAAACGCCCGAGAAGGGATCGCCGCCGGGAAGGACAAAATgg                                                                                          >  2:152106/1‑150 (MQ=255)
                                                               gcgcGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTg                                                                                           <  1:794334/148‑1 (MQ=255)
                                                               gcgcGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGa                                                                                         <  2:2025587/150‑1 (MQ=255)
                                                                cgcgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGaaa                                                                                       <  1:890817/151‑1 (MQ=255)
                                                                cgcgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGaaa                                                                                       <  2:1870148/151‑1 (MQ=255)
                                                                cgcgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGaaa                                                                                       <  1:681662/151‑1 (MQ=255)
                                                                cgcgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGG‑TCCTGCGATCCGGGAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGaa                                                                                        >  2:766732/1‑150 (MQ=255)
                                                                 gcgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAAc                                                                                      <  1:2034/151‑1 (MQ=255)
                                                                 gcgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGaa                                                                                                     >  1:1835703/1‑136 (MQ=255)
                                                                   gATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGc                                                                                    <  2:1731432/151‑1 (MQ=255)
                                                                         gCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGaaa                                                                               >  1:2025275/1‑150 (MQ=255)
                                                                           gAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAagt                                                                            <  1:1695650/151‑1 (MQ=255)
                                                                              ggTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAagtagt                                                                         <  2:329736/151‑1 (MQ=255)
                                                                               gTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTc                                                                        <  1:1093293/151‑1 (MQ=255)
                                                                               gTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGA‑ACCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAagtag                                                                          >  2:1640589/1‑149 (MQ=255)
                                                                                 ccAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTg                                                                      <  2:635384/151‑1 (MQ=255)
                                                                                 ccAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGA‑TCCTGCGATCCGGCAAAAACCCCCGAGAAGTGATCGCCGCCGTGGAGGACAAACTGGGAACGCTGGAAAGTAGTCt                                                                       >  2:372983/1‑150 (MQ=255)
                                                                                  cAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGc                                                                     <  1:1031441/151‑1 (MQ=255)
                                                                                  cAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGc                                                                     <  1:644298/151‑1 (MQ=255)
                                                                                  cAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGA‑TCCCGCGATCCGGCAAAAACGCCCGAGAAGGGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGGAAAGTAGTCTg                                                                      >  2:904526/1‑150 (MQ=255)
                                                                                       tGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGTGA‑GCCTGCGGTCCGGCCAAAACGCCCGAGAAGTGAACCCCCCCGTGAAGGAAAAACAGGGAACGCCCAAAaggagt                                                                         >  1:1711770/1‑142 (MQ=255)
                                                                                        ggCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGaa                                                                <  1:890408/150‑1 (MQ=255)
                                                                                         gCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAgg                                                              <  2:1641160/151‑1 (MQ=255)
                                                                                                gaTGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGgag                                                       <  1:2165090/151‑1 (MQ=255)
                                                                                                gaTGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGA‑TCCTGCGATCCGGCAAAAAAGCCCGAGAAGGGATCGCCGCCGTGAAGGACAAAATGGAAAAGCTGAAAAGGAGTCTGCCGGAAggggggggg                                                       >  2:552079/1‑144 (MQ=255)
                                                                                                  tGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGTGGTGG‑TACTGCGATTCCGGAAAAAAGCCCCGGAAATGAACGCCGCCCTGAAAGACAAACTTGGAACGCCGAAAAGTAGTCTGCCCGGAGGCGTgggaa                                                      >  1:1503316/1‑147 (MQ=255)
                                                                                                   gcgcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGAt                                                     <  2:474910/150‑1 (MQ=255)
                                                                                                            cATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGTGG‑TCCCGCGATCCGGCCAAAAAGCCCGGGAAGGGATCGCCGCCGGGGAGGACAAACTGGAAAAGCTGAAAAGTAGTCTGCCGGAAGGGGGGGGGATAGGTacaa                                             >  2:52479/1‑149 (MQ=255)
                                                                                                              ttGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGGGATCGCCGCCGTGAAGGACAAACTGGAAAAGCTGAAAAAGAGTCTGCCGGAAGGCGTGGGGATAGTTacaaca                                           >  1:1700547/1‑149 (MQ=255)
                                                                                                                gCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATAc                                        <  1:1665067/150‑1 (MQ=255)
                                                                                                                  cGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACg                                       >  1:566487/1‑149 (MQ=255)
                                                                                                                  cGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGGGGAGATAGTTACAACATACGa                                      >  1:1030748/1‑150 (MQ=255)
                                                                                                                   gAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGG‑TACCACCATCCCGCAAAAACGCCCGGGAAGGGATAGCCGCCGCGAAAGGCAAACTGGGAACGCTGAAAAGTAGTCTGCCGGGAGGGGTGGAGAAAGTTTCAACCTACGa                                      >  2:727814/1‑149 (MQ=255)
                                                                                                                    aaCTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGTGA‑TCCTGCGATTCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGGAACGCTGAAAAAAAGTCTGCCGGGAGGCGGGGAGATAGTTACAACATACGATc                                    >  1:333882/1‑150 (MQ=255)
                                                                                                                    aaCTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGA‑TCCTGCGATCCGGCAAAAAAGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGGAAAGGAGTCTGCCGGGAGGCGTGGGGATAGTTACAACATAc                                        >  2:2158379/1‑146 (MQ=255)
                                                                                                                     aCTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGc                                  <  2:701609/151‑1 (MQ=255)
                                                                                                                      cTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGTGA‑TCCTGCGATCCGGCAAAAAAGCCCGAGAAGTGATCGCCGCCGGGGAGGACAAAATGGGAACGCTGGAAAGTAGTCTGCCGGGAGGCGTGGGGATAGTTACAACATAAGAACg                                   >  2:993692/1‑149 (MQ=255)
                                                                                                                          aCGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGcagcca                             <  1:1988092/151‑1 (MQ=255)
                                                                                                                            ggcgaaggcgaaGTGGC‑GGGCGGGGGGGTGA‑TCCTGCGATCCGGCAAAAAAGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGcagcca                             >  1:366581/1‑149 (MQ=255)
                                                                                                                             gcgaaggcgaagTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCt                          <  1:1464410/151‑1 (MQ=255)
                                                                                                                             gcgaaggcgaagTGGC‑GGGCGGGGGGGGGA‑TCCTGCGATCCGGCAAAAACGCCCGAGGAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGGAGTCTGCCGGGAGGCGGGGAGATAGTTACAACATACGATCGCAGCCAGCt                          >  1:956004/1‑151 (MQ=255)
                                                                                                                               gaaggcgacgTGGC‑GGGCGGGGGGGTGA‑ACATGCGCCCCGGCAAAAAAGCCCGAGGAGGGGTCGGCGGCGTGAAGGACAAACTGGGAACGCTGGAAAGTAGCCTGCCCGCAGGCGTGGGGGGAGGTACAACATACGATCGcagccagccc                         >  2:2087286/1‑148 (MQ=255)
                                                                                                                               gaaggcgaagTGGC‑GGGGGGGGGGGGGA‑TCCTGCGATCCGGCAAAAACGCCCGGGAAGGGATCGCCGCCGTGAAGGAAAAAATGGGAACGCTGAAAAGGAGTCTGCCGGAAGGCGGGGAGATAGTTAAAACATACGATCGCAGCCAGCTc                         >  2:1592795/1‑150 (MQ=255)
                                                                                                                                              gggcgggGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATc          <  1:249338/151‑1 (MQ=255)
                                                                                                                                               ggcgggGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCg         <  2:1304089/151‑1 (MQ=255)
                                                                                                                                                  ggggTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGAc       >  1:300493/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                       
AACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGA‑TCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACCT  >  NC_000913/599427‑599726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑