Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913993,9130GC43.1% 256.0 / 57.7 144G44G (GGC→GGG) ssuBaliphatic sulfonate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base G (75/7);  new base C (0/62);  total (75/69)
Fisher's exact test for biased strand distribution p-value = 2.91e-33
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 147 total aligned reads displayed.

CCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTC  >  NC_000913/993763‑994059
                                                                                                                                                           |                                                                                                                                                  
ccACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCgg                                                                                                                                                    <  2:1120206/151‑1 (MQ=255)
ccACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCgg                                                                                                                                                    <  1:1168464/151‑1 (MQ=255)
     gTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCccac                                                                                                                                               <  1:1168470/151‑1 (MQ=255)
        gCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCcaccac                                                                                                                                            <  1:1466930/151‑1 (MQ=255)
          aGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCg                                                                                                                                          <  1:1283442/151‑1 (MQ=255)
                   tCCTGAAACATCATTCGCGTGTCTTCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACt                                                                                                                                 >  1:1358692/1‑151 (MQ=255)
                    ccTGAAACATCATTCGCGTGTCTTCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTg                                                                                                                                >  2:341891/1‑151 (MQ=255)
                    ccTAAAACATCTTCCGCGTGTCTCCCTGAATTC‑CACCCGGGGGTGTGGCCCCCGCAAACACACCGCCGGCGGTT‑GGCTTTCCCAGCCCTGCCAGCAGGCGCAGCAGGGTAT‑TTTG‑C‑CCCCCCCCGCTGCGGCCCCCCACCGCCACAAACTg                                                                                                                                <  2:124620/151‑1 (MQ=255)
                       gAAACATCATTCGCGTGTCTTCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGAcc                                                                                                                             >  1:1106900/1‑151 (MQ=255)
                          aCATCATTCGCGTGTCTTCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGc                                                                                                                          >  2:830601/1‑151 (MQ=255)
                           catcatTCGCGTGTCTTCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGcc                                                                                                                         >  2:540306/1‑151 (MQ=255)
                              ctttCGCTTTTCTCCCTAATTTC‑CACCCAGCGGTGGGGTGCCCCCTAACACCCCGCCTGCGTTT‑GGCGTTCCCACCCCTGCCAGCAGGCGCGGCGGGGT‑C‑TTTT‑GCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGc                                                                                                                          <  1:379462/145‑1 (MQ=255)
                               atTCGCGTGTCTTCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGa                                                                                                                      >  1:1947834/1‑150 (MQ=255)
                                  cgctTGTTTTCCTAATTTC‑CAGCCAGGGGTGGGGGCCCCGCAACCACA‑CCCCTGGGGTTGGGCTTTTCCAGCCCTGCCAGCGGGCGCAGCAGGTTAT‑TTTT‑G‑CCACCCCCGCGGGGCCCCACCTCCGCCACAAACTGACCTGCCg                                                                                                                        <  1:1019463/145‑1 (MQ=255)
                                  cgcgTGTCTTCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAatat                                                                                                                  >  2:1128488/1‑151 (MQ=255)
                                   gcgTTTCTCCCTGATTTC‑CAGCCAGCGGTGGGGCCCCCACAACCCCCCCCCCGGCGGTT‑GGCTTTCCCACCCCTCCCAGCGGGCGCAGCGGGGTTC‑TTTC‑C‑CCCCCACCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGa                                                                                                                      <  2:841296/146‑1 (MQ=255)
                                   gcgTGTCTTCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATg                                                                                                                 >  1:2050040/1‑151 (MQ=255)
                                    cgTGTCTTCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGt                                                                                                                >  1:28539/1‑151 (MQ=255)
                                      ttttttCCTAATTTC‑CAGCCGGGGGTGTGGTGCCCGCTAACACCCCGCCGGGGGTG‑GGCTTTCCCAGCCCTGCCAGCGGGCGCAGCGGGTTTC‑TTTC‑C‑CCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATg                                                                                                                 <  2:1332309/144‑1 (MQ=255)
                                      tgtCTTCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTa                                                                                                               >  1:510598/1‑150 (MQ=255)
                                      tgtATTCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTa                                                                                                               >  2:423855/1‑150 (MQ=255)
                                       gtCTTCTTAATTTC‑CGGCCGGCGGTGTGGGCCCCCCAACCCCCCCGCCGGGGTTT‑GGCTTTCCCGGCCCTGCCGGCGGCCGAAGCGGGTTCC‑TTTC‑C‑CCACCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAat                                                                                                                    <  1:991945/144‑1 (MQ=255)
                                        tCTTCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAAt                                                                                                            >  1:241713/1‑151 (MQ=255)
                                        tCTTCCTAAATTC‑CACCGGGGGGGGTGGCCCCCGAAAACACACCCCGTGGGGTG‑GGCTTTCCCAGCCCGGCCAGCGGGCGAAGAGGGGTTT‑TTGG‑C‑CCCCCCCCGCGGCGCCCCACCACCGCCACAAACTGACCTGCCGGAATATGTa                                                                                                               <  2:402216/148‑1 (MQ=255)
                                          ttCTTGATTTC‑CAGCCGGGGGTGGGGTGCCCGCAAACCCACCGCCGGGGGTG‑GGCGTTCCCAGCCCTGCCAGCGGGCGCAGGAGGTTCC‑TTTC‑C‑CCACCACCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATg                                                                                                                 <  2:1350491/144‑1 (MQ=255)
                                          ttCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATcc                                                                                                          >  1:2116291/1‑151 (MQ=255)
                                           tccctaaTTTCCAGCCGGGGGTGGGGGCCCCGTAAACACACCGCCGGGGTTT‑GGCTTTCCCAGCCCTCCCAGCGGGCGCAGCGGGGTTT‑TTTT‑G‑CC‑CCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTaaa                                                                                                             <  1:1064991/142‑1 (MQ=255)
                                            ccTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATcc                                                                                                          >  2:1111565/1‑149 (MQ=255)
                                            ccTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATcc                                                                                                          >  1:1304636/1‑149 (MQ=255)
                                            ccTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATcc                                                                                                          >  2:1595526/1‑149 (MQ=255)
                                                aaTTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTgg                                                                                                    >  1:2116129/1‑151 (MQ=255)
                                                  ttt‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGtt                                                                                                  >  2:1253896/1‑151 (MQ=255)
                                                   ttccagccaggGGTGTGGTGCCCGCTAACACATCGCCTGGGGTT‑GGCTTTCCCAGCCCTCCCAGCAGGCGCAGGGGGTTAT‑TTTT‑C‑CCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGt                                                                                                   <  2:666155/147‑1 (MQ=255)
                                                     ccagccgggGGTGGGGGCCCCGCAACCACACCCCCGGGGGTG‑GGCTTTCCCGGCCCGCCCAGAGGGCGCAG‑AGGGTTCTTTTT‑C‑CCCCCCCCGCGGCGCCCCCCCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCa                                                                                                <  2:1704281/150‑1 (MQ=255)
                                                     ccagccgggGGGGGGGTCCCCGCTAACACACCGCCTGCGTTT‑GGCTTTCCCAGCCCTGCCAGCAGGCGCAGAAGGTTTT‑TTTG‑C‑CCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAACCCAGTTGGTTCa                                                                                                <  2:407797/150‑1 (MQ=255)
                                                       agccagcGGTGTGGTGCCCGCTAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAgg                                                                                              >  1:259519/1‑151 (MQ=255)
                                                       agccagcGGTGTGGTGCCCGCTAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAgg                                                                                              >  2:1212918/1‑151 (MQ=255)
                                                         ccaggGGTGGGGTCCCCGCAAACACACCCCCGGCGGTG‑GGCTTTCCCAGCCCTCCCAGCGGGCGCAGCGGGGTTT‑TTTG‑C‑CCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGa                                                                                             <  1:441427/150‑1 (MQ=255)
                                                           agcGGTGTGGTGCCCGCTAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACg                                                                                           >  2:1393696/1‑150 (MQ=255)
                                                            ggggtgggggccccGCAACCACACCGCGGGGGGTG‑GGCTTTCCCAGCCCCGCCGGCGGGCGCAGGGGGTTTT‑TTTT‑C‑CCCCCACCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAgg                                                                                              <  2:1830878/135‑1 (MQ=255)
                                                              ggTGTGGTCCCCGCAAACACATCGCCGGGGGTG‑GGCTTTCCCACCCCTGCCAGCGGGCGAAGCGGGTTAT‑TTTT‑C‑CCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGata                                                                                        <  2:1470114/150‑1 (MQ=255)
                                                                 gtggtgCCCGCTAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATAtttt                                                                                    >  1:765162/1‑151 (MQ=255)
                                                                 gtggtcCCCGCTAACCCACCGCCTGGGTTG‑GGCGTTCCCAGCCCTGCCAGCGGGCGCAGCGGGGTAT‑TTTT‑C‑CCCCCCCCGCTGCGCCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATAtttt                                                                                    <  1:182532/151‑1 (MQ=255)
                                                                    gtgCCCGCTAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCcg                                                                                 >  2:2075624/1‑151 (MQ=255)
                                                                       cccGCTAACACACCGCCTGCGGTT‑GGCTTTTCCAGCCCTCCCAGCAGGCGCAGCGGGTTCT‑TTTT‑C‑CCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCcg                                                                                 <  1:235971/148‑1 (MQ=255)
                                                                       cccGCAAACCCACCGCCTGGGGTG‑GGCTTTCCCAGCCCTGCCGGCAGGCGCAGCGGGGTAT‑TTTT‑C‑CCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCcgc                                                                                <  1:810824/149‑1 (MQ=255)
                                                                        ccGCTAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGt                                                                              >  1:717518/1‑150 (MQ=255)
                                                                          gCTAACACATCGCCTGCGGAT‑GGCGTTTCCAGACCTGCCAGCAGGAGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTaa                                                                            >  1:943414/1‑150 (MQ=255)
                                                                            tAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGt                                                                         >  2:1699597/1‑151 (MQ=255)
                                                                             aaCCCATCGCCGGGGGTT‑GGCTTTTCCAGACCTGCCAGCGGGCGCAGCGGGGTTT‑TTTT‑G‑CCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATg                                                                          <  1:85638/149‑1 (MQ=255)
                                                                             aaCACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGtt                                                                        >  1:951272/1‑151 (MQ=255)
                                                                             aaCACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGtt                                                                        >  2:449371/1‑151 (MQ=255)
                                                                              acacATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGttt                                                                       >  2:673392/1‑151 (MQ=255)
                                                                               cacaTCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTg                                                                      >  2:2068006/1‑151 (MQ=255)
                                                                               cacaTCCCCTGCGGTG‑GGCGTTCCCACCCCGGCCAGCGGGCGCAGCGGGTTTC‑TTTC‑C‑CCACCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGttt                                                                       <  1:886580/150‑1 (MQ=255)
                                                                                acaTCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGc                                                                     >  1:496385/1‑151 (MQ=255)
                                                                                  acccccgggggTG‑GGCTTTCCCAGCCCTCCCAGCAGGCGCGGCAGGTTCT‑TTTT‑C‑CCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTg                                                                      <  1:415770/139‑1 (MQ=255)
                                                                                    ccccTGCGGTG‑GGCGTTCCCACCCCCGCCAGCAGGCGCAGCAGGTTAC‑TTTC‑C‑CCCCCCCCTTTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTAc                                                                 <  1:289172/149‑1 (MQ=255)
                                                                                      cgggCGGTG‑GGCTTTCCCAGCCCTCCCAGCGGGCGCAG‑AGGGTTT‑TTTTCC‑CCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACt                                                                <  2:1655139/147‑1 (MQ=255)
                                                                                           ggTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTg                                                          >  2:2358/1‑151 (MQ=255)
                                                                                           ggTG‑GGCTTTCCCAGCCCCCCCAGCAGGCCCAGCGGGGTTT‑TTTC‑C‑CCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTg                                                               <  2:350091/146‑1 (MQ=255)
                                                                                              t‑GGCGTTCCCAGCCCTGCCAGCAGGCGCAGCGGGTTAC‑TTTT‑C‑CCCCCCCCGCTGCGCCCCCCCGCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCa                                                             <  1:1906328/145‑1 (MQ=255)
                                                                                                 gtgTTCCCAGCCCGGCCAGCGGGCGCAGCGGGGTTC‑TTTT‑C‑CCACCCCCGCTGCGGCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGc                                                       <  1:700305/147‑1 (MQ=255)
                                                                                                   gTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGcaac                                                    >  2:1985025/1‑150 (MQ=255)
                                                                                                      tCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATg                                                >  1:1955153/1‑151 (MQ=255)
                                                                                                      tCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATg                                                >  2:572232/1‑151 (MQ=255)
                                                                                                      cccacccccgCCGGCGGGCGCAGGAGGTTTT‑TTTT‑C‑CCCCCCCCCCGGCGCCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATg                                                <  2:25048/142‑1 (MQ=255)
                                                                                                      cccaccccTGCCAGCGGGCGCGGGGGGTTTT‑TTTT‑C‑CCCCCCCCGCGGGGCCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGcaacaa                                                  <  2:1978570/143‑1 (MQ=255)
                                                                                                       ccAGCCCTGCCAGCAGGCGCAGCAGGGTTC‑TTTT‑C‑CCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATgg                                               <  1:1324198/151‑1 (MQ=255)
                                                                                                         aGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCg                                             >  1:1124775/1‑151 (MQ=255)
                                                                                                            ccggCCAGCGGGCGCAGGGGGGTAC‑TTTC‑C‑CCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGcaacaa                                                  <  2:1892426/140‑1 (MQ=255)
                                                                                                            ccggCCAGCAGGCGCAG‑AGGGTTC‑TTTTGG‑CCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGc                                          <  1:1037365/148‑1 (MQ=255)
                                                                                                            ccTGCCAGCGGGCGCAGCAGGGTCC‑TTTT‑C‑CCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAAt                                                 <  1:1962853/144‑1 (MQ=255)
                                                                                                              tGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGccc                                        >  1:662557/1‑151 (MQ=255)
                                                                                                              ggCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGccc                                        >  2:2082754/2‑151 (MQ=255)
                                                                                                                   gcagGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGtt                                   >  2:2098805/1‑151 (MQ=255)
                                                                                                                    cgggCGCAGCGGTGTAC‑TTTC‑C‑CCCCCCCCGCTGCGCCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGtt                                   <  2:1535222/148‑1 (MQ=255)
                                                                                                                    cagGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTc                                  >  1:1469437/1‑151 (MQ=255)
                                                                                                                      ggCGCAGCGGGGTTC‑TTTG‑C‑CCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCa                                 <  1:414022/150‑1 (MQ=255)
                                                                                                                      ggCGCAGCAGGGTCT‑TTTC‑C‑CCCCCACCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTgg                                     <  1:478407/146‑1 (MQ=255)
                                                                                                                           agcagGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACg                             >  2:924855/1‑149 (MQ=255)
                                                                                                                           agcagGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACg                             >  1:1192057/1‑149 (MQ=255)
                                                                                                                            gcagGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGc                          >  2:1495102/1‑151 (MQ=255)
                                                                                                                             gagGGTTC‑TTTTCC‑CCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGAc                              <  1:1575089/146‑1 (MQ=255)
                                                                                                                             cagGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGc                          >  1:1545094/1‑150 (MQ=255)
                                                                                                                              agGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGTCGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGc                          >  1:1766246/1‑149 (MQ=255)
                                                                                                                               ggttcc‑TTTT‑C‑CCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGc                          <  1:1144801/143‑1 (MQ=255)
                                                                                                                               ggttcc‑TTTT‑C‑CCACCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAg                        <  1:992605/145‑1 (MQ=255)
                                                                                                                               gggTAT‑TTTC‑G‑CCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCa                         <  1:746077/149‑1 (MQ=255)
                                                                                                                                ggttattttt‑C‑CCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGa                            <  1:480482/140‑1 (MQ=255)
                                                                                                                                gggttttttt‑C‑CCCCCCCCGCGGCGCCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCa                         <  2:1555369/143‑1 (MQ=255)
                                                                                                                                      tttt‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCAt                 >  1:84560/1‑151 (MQ=255)
                                                                                                                                       ttt‑C‑CCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCAt                 <  1:168756/150‑1 (MQ=255)
                                                                                                                                        tt‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATAc               >  1:1789412/1‑151 (MQ=255)
                                                                                                                                             ccaccaCCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGt            >  1:1337145/1‑151 (MQ=255)
                                                                                                                                                 caccGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGcc        >  2:938000/1‑151 (MQ=255)
                                                                                                                                                     gCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGcctcct    >  2:1234276/1‑151 (MQ=255)
                                                                                                                                                      cTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCtt   >  1:1161803/1‑151 (MQ=255)
                                                                                                                                                       tGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTc  >  2:1683653/1‑151 (MQ=255)
                                                                                                                                                        gCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTc  >  2:2167541/1‑150 (MQ=255)
                                                                                                                                                           |                                                                                                                                                  
CCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTT‑GGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTAC‑TTTT‑G‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTC  >  NC_000913/993763‑994059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑