Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,434,8820TG45.9% 216.5 / 31.8 122Y252S (TAC→TCC) truAtRNA pseudouridine(38‑40) synthase
Reads supporting (aligned to +/- strand):  ref base T (9/57);  new base G (56/0);  total (65/57)
Fisher's exact test for biased strand distribution p-value = 1.93e-25
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

CAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATAT  >  NC_000913/2434733‑2435032
                                                                                                                                                     |                                                                                                                                                      
cAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTa                                                                                                                                                       <  1:721835/151‑1 (MQ=255)
  ggTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGTCAGGGGaa                                                                                                                                                      >  2:853271/1‑150 (MQ=255)
   gTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGaa                                                                                                                                                      >  2:921837/1‑149 (MQ=255)
       atatTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCAcc                                                                                                                                                <  2:1565/151‑1 (MQ=255)
       atatTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCAcc                                                                                                                                                <  1:1284906/151‑1 (MQ=255)
          tttGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCCGGCAGGGGAATCCAcccc                                                                                                                                              >  1:1994929/1‑148 (MQ=255)
           ttGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGGCAGGGGAATCCAcc                                                                                                                                                >  2:1306812/1‑147 (MQ=255)
            tGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCCGGCAGGGGAATCCAcccc                                                                                                                                              >  2:1793809/1‑146 (MQ=255)
             gCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATAACCGGCCGGGGAATCcccccc                                                                                                                                              >  2:941837/1‑145 (MQ=255)
             gCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGTGAGGGGAATCCAcc                                                                                                                                                >  2:1753906/1‑145 (MQ=255)
             gCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGAc                                                                                                                                           <  2:869838/150‑1 (MQ=255)
              ccTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCCCGAcc                                                                                                                                          >  2:512906/1‑150 (MQ=255)
                 aTGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGGAATCCACCCCGAcc                                                                                                                                          >  2:1605958/1‑147 (MQ=255)
                 aTGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGa                                                                                                                                       <  1:1673483/150‑1 (MQ=255)
                 aTGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCCCGACCAGa                                                                                                                                       >  2:372949/1‑150 (MQ=255)
                    ccTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACa                                                                                                                                   <  2:2071025/151‑1 (MQ=255)
                    ccTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACa                                                                                                                                   <  2:16799/151‑1 (MQ=255)
                     cTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGTCCGGGGAATCCCCCCCGACCCGATACa                                                                                                                                   >  1:1334966/1‑150 (MQ=255)
                        gTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGccc                                                                                                                               <  2:1250041/151‑1 (MQ=255)
                        gTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGccc                                                                                                                               <  1:2137297/151‑1 (MQ=255)
                         tACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATAACGGTCAGGGGAATCCACAGCGACCCGATACAgac                                                                                                                                >  1:501406/1‑147 (MQ=255)
                          aCTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCtt                                                                                                                             <  2:240565/151‑1 (MQ=255)
                             cGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTc                                                                                                                            <  2:1840340/149‑1 (MQ=255)
                             cGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTc                                                                                                                            <  2:1904265/149‑1 (MQ=255)
                             cGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCg                                                                                                                          <  2:248123/151‑1 (MQ=255)
                              gTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCGc                                                                                                                         >  1:826258/1‑151 (MQ=255)
                               tCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCCGTCAGGGGAATCCACCCCGACCAGATACAGCCCTTCCGc                                                                                                                         >  1:1269756/1‑150 (MQ=255)
                                cATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCt                                                                                                                        <  2:1223873/150‑1 (MQ=255)
                                  tCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCGCtttt                                                                                                                     >  2:633921/1‑151 (MQ=255)
                                     ttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCttt                                                                                                                      <  1:549688/147‑1 (MQ=255)
                                     ttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTg                                                                                                                    <  1:694617/149‑1 (MQ=255)
                                     ttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGcc                                                                                                                  >  1:926151/1‑151 (MQ=255)
                                       cAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTTcc                                                                                                                  >  1:421187/1‑149 (MQ=255)
                                        aGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCCGGGGAATCCCCCCCGACCAGATACAGCCCTTCCGCTTTTGCCGtt                                                                                                               >  1:2039361/1‑151 (MQ=255)
                                        aGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGtt                                                                                                               <  2:1320194/151‑1 (MQ=255)
                                        aGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAACCCTTCCGCTTTTTCCGtt                                                                                                               >  1:1488196/1‑151 (MQ=255)
                                          gCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTtgc                                                                                                             <  1:1631698/151‑1 (MQ=255)
                                           cTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCCCGACCAGATACAGCCCTTCCGCTTTTTcc                                                                                                                  >  1:1516560/1‑145 (MQ=255)
                                            tGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGGCAGGGGAATCCACCGCGACCAGAAACAACCCCTCCGCTTTTTCCGtt                                                                                                               >  1:2017964/1‑147 (MQ=255)
                                             gTAACTACGTTGGCTACAGACTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTtgct                                                                                                            <  1:1769647/149‑1 (MQ=255)
                                              tAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCCGGCAGGGGAATCCACCCCGACCAGATAAAGCCCCTCCGCTTTTTCCGTTGCTgcc                                                                                                         >  1:1060340/1‑151 (MQ=255)
                                                aCTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTgccgc                                                                                                       <  2:120512/151‑1 (MQ=255)
                                                 cTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATAACCGGCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCCCTTTTTCCCTTGCTgccgcc                                                                                                      >  2:1042578/1‑151 (MQ=255)
                                                  tACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTgccgcc                                                                                                      >  1:326952/1‑150 (MQ=255)
                                                   aCGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCa                                                                                                     <  1:196619/150‑1 (MQ=255)
                                                         gCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTTCCGTTGCTGCCGCCAGCGGTc                                                                                               >  1:317320/1‑150 (MQ=255)
                                                           tACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGAACAAAACGGTCAGGGGAATCCACCCCGGCCCGAAACAGCCCTTCCGCTTTTTCCCTTGCTTCCGCCCGCGTTCt                                                                                              >  2:1136525/1‑149 (MQ=255)
                                                            aCAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAAAACAAAACCGGCAGGGGAATCCACCCCCACCAGAGAAAGACCTTCCCCCTTTTCCGTTTCTGTCGCCAACGTTCTGt                                                                                            >  2:1118162/1‑150 (MQ=255)
                                                              aGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATTATACCGGGCAGGGGAATCCACCCCGACCAGATACAGCCCTTCCGCTTTTTCCGTTGCTGCCGCCAGCGTTCTGTcc                                                                                          >  1:1280694/1‑150 (MQ=255)
                                                               gCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAACCCTTCCGCTTTTTCCGGTGCTGCCGCCAGCGTTCTGTCCtt                                                                                        >  1:1285715/1‑151 (MQ=255)
                                                                ccTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCttt                                                                                       <  1:1338128/151‑1 (MQ=255)
                                                                  tGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGGTGCTGCCGCCCGCGTTCTGTCCttt                                                                                       >  1:1409093/1‑149 (MQ=255)
                                                                   gAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTg                                                                                      <  2:2066594/149‑1 (MQ=255)
                                                                     aaTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGGCAGGGGAAACCACCCCGACCAGATACAACCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTtccgc                                                                                  >  1:1392656/1‑151 (MQ=255)
                                                                      aTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATAACGGGCAGGGGAAAACACCCCGACCAGATACAACCCTTCCGCTTTTTCCGGTGCTGCCCCCCGCGGTCTGTCCTTTgccccc                                                                                 >  2:600145/1‑151 (MQ=255)
                                                                      aTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGGTTTTGTAGATTATATCGGGCAAGGGAATCCACCCCGAACTGATAAAGCCCTTTCGCATTTTTGGCTGTTGCCCCCCGAGGTCTGTTCTTTgccccc                                                                                 >  2:753890/1‑151 (MQ=255)
                                                                           ccGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGTCAGGGGAATCCACCCCGACCAGATACAACCCTTCCGCTTTTTCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGccgcccgc                                                                              >  1:491144/1‑149 (MQ=255)
                                                                           ccGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCa                                                                             <  2:1071544/150‑1 (MQ=255)
                                                                            cGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCCGGGGAATCCACCCCGACCAGATACAGCCCCTCCCCTTTTTCCGTTGCTGCCGCCCGCGGTCTGTCCTTTTCCGCCCGCaa                                                                            >  1:813061/1‑150 (MQ=255)
                                                                            cGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGTAGAGCATACCGGTTAGGGGAATACACCGCCGCCAAAAACAGCCCTTCCACCTTTTCCCGTGTTGCCGCCAGCGTTCTGTTCTTTtccgcc                                                                                 >  1:1130522/1‑145 (MQ=255)
                                                                             gAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAAct                                                                          <  1:2057348/151‑1 (MQ=255)
                                                                                cATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAActct                                                                        <  2:1067695/150‑1 (MQ=255)
                                                                                cATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTg                                                                       <  1:1748792/151‑1 (MQ=255)
                                                                                    attCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTAt                                                                   <  2:476850/151‑1 (MQ=255)
                                                                                      tctcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCCGTCAGGGGAAACCACCCCGACCAGATACAGCCCCTCCGCTTTTTCCGTTGCTGCCGCCAGCGTTCTGTCCTTTTCCGCCAGCAAATCTGCTATcc                                                                 >  1:1198932/1‑151 (MQ=255)
                                                                                        tcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGTCCGGGGAATACACCGCGACCAAATACAGCCCCTCCGCTTTTTCCGGTGCTGCCGCCCGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATcc                                                                 >  1:1613503/1‑149 (MQ=255)
                                                                                             aGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGctct                                                           <  2:327312/150‑1 (MQ=255)
                                                                                               tCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCCGTCAGGGGAATCCACCGCGACCAGATACAGCCCCTCCGCTTTTTCCGTTGCTGCCGCCAGCGTTCTGTCCTTTTCCGCCCGCAACTCTGCTATCCAGCTCTcc                                                         >  1:9471/1‑150 (MQ=255)
                                                                                               tCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCCGGCAGGGGAATACACCCCGGCCAGATACAGCCCCTCCGCTTTTGCCGTTGCTGCCGCCAGCGGTCTGTCCTTTGCCGGCAGCAACTCTGCTATCCAGCTCTcc                                                         >  1:476237/1‑150 (MQ=255)
                                                                                                ccGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCg                                                        <  1:662090/150‑1 (MQ=255)
                                                                                                 cGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCCGTCAGGGGAATCCACCGCGACCAGATAAAACCCTTCCGCTTTTTCCGGTGCTGCCGCCAGCGTTCTGTTCTTTGCCGCCCGCCACTCTGCTATCCAGCTCTCCgg                                                       >  1:617513/1‑150 (MQ=255)
                                                                                                  gCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCt                                                     <  2:938655/151‑1 (MQ=255)
                                                                                                   ccAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTg                                                    <  1:1364911/151‑1 (MQ=255)
                                                                                                    cAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGTCAGGGGAATCCACCGCGACCCAAAAAAACCCTTCCCCTTTTTCCGTTGCTGCCGCCCGCGTTCTGTCCTTTTCCCCCAGCAACTCTGCTATCCAGCTCTCCCGCTgg                                                   >  1:1375897/1‑151 (MQ=255)
                                                                                                     aGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAAACCACCCCGACCAGATACAACCCTTCCCCTTTTTCCGTTGCTGCCGCCAGCGTTCTGTCCTTTTCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTgg                                                   >  1:2109508/1‑150 (MQ=255)
                                                                                                      gAAATAGCGGGCCCATTGGCGGTTTTGGGAGATTATACCGGTCAGGGGAATACACCGCGACCAGATACAGCCCTTCCGCTTTTTTCGTTGCTGCCGCCCGCGGTCTGTCCTTTGCCGCCCGCAACTCTGCTATCCAGCTCTCCGGCTGGt                                                  >  2:656199/1‑150 (MQ=255)
                                                                                                        aaTAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCCGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTTCGCTTTTTTCGTTGCTGCCCCCCGCGTTCTGTCCTTTTCCGCCCGCAACCCTGCTATCCCGCTCTCCCGCTGGTtgt                                               >  1:506437/1‑151 (MQ=255)
                                                                                                         aTAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAATCCACCCCGGCCAGATACAGCCCCTCCCCTTTTGCCGTTGGTGCCCCCCGCGTTCTGTCCTTTGCCCCCCGCAACTCTGCTATCCAGCTCTCCCGCTgggt                                                 >  1:906505/1‑146 (MQ=255)
                                                                                                         aTAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTtgtg                                              <  2:1105671/151‑1 (MQ=255)
                                                                                                            gCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGc                                           <  2:1027032/151‑1 (MQ=255)
                                                                                                              gggCCCATTGGCGGTTTTTGGAGAATATAACGGTCAGGGGAATCCACCGGGACCAGATAAAGCCCTTTCCCTTTTTCCGTTGCTGCCGCCCGCGTTCTGTTCTTTTCCGCCAGCCACTCTTCTATCCAGCTCTCCGGCTGGTTGTggg                                            >  2:1300067/1‑148 (MQ=255)
                                                                                                                  ccATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAc                                     <  2:1599728/151‑1 (MQ=255)
                                                                                                                     ttGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTc                                  >  1:1631815/1‑151 (MQ=255)
                                                                                                                       ggCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCa                                <  1:344361/151‑1 (MQ=255)
                                                                                                                         cGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATc                              >  1:2007908/1‑151 (MQ=255)
                                                                                                                         cGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATc                              <  2:496639/151‑1 (MQ=255)
                                                                                                                             tttGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGc                          >  1:797716/1‑151 (MQ=255)
                                                                                                                              ttGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGTACCGACTTCCATCAGGc                          <  2:734784/150‑1 (MQ=255)
                                                                                                                                 gAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGcc                      <  1:1793843/151‑1 (MQ=255)
                                                                                                                                 gAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGcc                      >  2:270467/1‑151 (MQ=255)
                                                                                                                                  aaGATCATACCGGTCAGGTTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCg                     <  1:1309357/151‑1 (MQ=255)
                                                                                                                                  aaGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCg                     >  2:98332/1‑151 (MQ=255)
                                                                                                                                  aaGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCg                     <  1:150436/151‑1 (MQ=255)
                                                                                                                                           ccGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTc            <  1:721589/151‑1 (MQ=255)
                                                                                                                                             ggTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCt          >  1:556009/1‑151 (MQ=255)
                                                                                                                                                cAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGa        <  1:1803937/150‑1 (MQ=255)
                                                                                                                                                cAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAc       <  2:1950503/151‑1 (MQ=255)
                                                                                                                                                   ggTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCat    <  1:1135858/151‑1 (MQ=255)
                                                                                                                                                    gTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCata   <  1:262505/151‑1 (MQ=255)
                                                                                                                                                     tAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCatat  >  1:1373321/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
CAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATAT  >  NC_000913/2434733‑2435032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑